• 한국안광학회지
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• 제14권1호
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• pp.69-80
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• 2009

목적: 본 연구는 영국에서 개발된 컴퓨터 모니터용 검안프로그램 Test Chart 2000 Pro의 타당도(validity)를 평가하기 위해 수행되었다. 방법: Test Chart 2000 Pro와 한국표준검안법의 공통된 10종류 검안항목에 대하여, 각 항목별로 동일한 피실험자군을 대상으로 2가지 검안법을 함께 실시하고, 측정결과의 일치도를 비교분석하였다. 결과: 10종류의 비교실험 중 Snellen Chart, Cross-cyl Target, Duochrome Test, Fan and Block Test, Random Dot Stereograms에서는 한국표준검안법과 Test Chart 2000 Pro양측의 결과가 통계적으로 유의미한 일치를 보였으나, LogMar Chart, Single Letter Chart, Phoria Test, Fixation Disparity Test, Worth 4 Dot Test에서는 두 검안 방법의 결과에서 차이가 나타났다. 결론: 한천석 시력표와 포롭터로 이루어진 한국표준검안법을 기준으로 했을 때 Test Chart 2000 Pro의 타당도는 높지 않으며, 측정의 정확도 측면에서 개선이 요구된다.

• Toxicological Research
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• 제33권3호
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• pp.191-203
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• 2017

Human eyes and skin are frequently exposed to chemicals accidentally or on purpose due to their external location. Therefore, chemicals are required to undergo the evaluation of the ocular and dermal irritancy for their safe handling and use before release into the market. Draize rabbit eye and skin irritation test developed in 1944, has been a gold standard test which was enlisted as OECD TG 404 and OECD TG 405 but it has been criticized with respect to animal welfare due to invasive and cruel procedure. To replace it, diverse alternatives have been developed: (i) For Draize eye irritation test, organotypic assay, in vitro cytotoxicity-based method, in chemico tests, in silico prediction model, and 3D reconstructed human cornealike epithelium (RhCE); (ii) For Draize skin irritation test, in vitro cytotoxicity-based cell model, and 3D reconstructed human epidermis models (RhE). Of these, RhCE and RhE models are getting spotlight as a promising alternative with a wide applicability domain covering cosmetics and personal care products. In this review, we overviewed the current alternatives to Draize test with a focus on 3D human epithelium models to provide an insight into advancing and widening their utility.

• 한국환경성돌연변이발암원학회지
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• 제22권4호
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• pp.296-302
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• 2002

In recent years, the safety of sunscreens have been challenged based on the reports of its adverse effects on users; dermatitis, allergic contact dermatitis, photoallergic contact dermatitis. In the toxicological aspect, the need to investigate the safety of sunscreens is steadily increasing. However, there were few studies on the correlation between sun protection factor (SPF) and the safety of sunscreens. The objective of this study was to assess whether there was a correlation between SPF and the skin irritation index of sunscreens. Octyl methoxycinnamate (OMC) was selected, which was the most frequently used (about 92% in korea and about 90% in the world) in sunscreens. In vitro SPF test was performed using the standard samples recommended by KFDA guideline. We examined primary and cumulative skin irritation and eye irritation of OMC standard sample as well as homosalate. As in vitro SPF was increased, skin irritation index was increased in primary skin irritation test ($r^2$=0.986) and cumulative skin irritation test ($r^2$=0.939). In addition, OMC was non-irritating and non-sensitizing. In this results, there was a good correlation between SPF index and skin irritation index.

• 분석과학
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• 제34권6호
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• pp.275-283
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• 2021

본 연구에서는 화학 물질의 급성 안자극 시험을 수행하기 위한 세포 기반 미세유체 칩의 개발과 응용에 관한 연구를 수행하였다. 포토리소그래피와 소프트리소그래피 공정을 이용하여 미세유체 칩을 제작하였으며, 칩은 배양 면적이 다른 3개의 세포 배양 구획으로 이루어져 있다. 세포 기반 안자극 시험은 토끼 각막 상피 세포를 사용하여 수행하였다. 미세유체 칩에 배양된 세포에 화학 물질 수용액을 처리한 후 일정한 간격으로 세포를 관찰하고, 생존율 곡선을 기반으로 세포 사멸에 대한 속도 상수를 계산하였다. 세포-세포 사이의 연접, 세포-기판 사이의 부착, 초기 세포 수 변화가 세포 사멸 속도에 미치는 영향을 조사하여 미세유체 칩의 성능을 검증하였다. 안자극 시험의 표준물질인 sodium dodecylsulfate (SDS) 수용액의 다양한 농도 조건에서 안자극 시험을 수행하였다. 화학 물질의 수용액에 300초 동안 노출시킨 세포의 생존율을 이용하여 안자극을 시험하였다. 최종적으로 미세유체 칩의 각 구획에 대한 가중치를 기반으로 독성 점수(toxicity score, TS) 산출식을 얻었다. 본 연구에서 개발한 세포 기반 미세유체 칩은 화장품과 제약에 사용되는 화학 물질의 안자극 시험에 활용될 수 있을 것이다.

• 한방안이비인후피부과학회지
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• 제23권2호
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• pp.186-195
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• 2010

Objective : This study was performed to prove the effect of the oriental medical treatments like acupuncture, moxibustion, herbal medicine on macular edema resulted from branch retinal vein occlusion which could lead to terrible result like permanent visual loss at worst. Methods : The patient was treated 62 times, from July 15th, 2009 through May 12th, 2010. We used fundus photography and Dr. Hann's standard test chart for 5m to examinate the patient's eyesight. We used acupuncture, electropuncture, moxibustion and herbal medicine to treat the patient. We considered visual field, scotoma, metamorphopsia and vitreous floater to measure the effect of treatment. Results : After about 10 months of treatment, the eyesight of Rt eye was improved from 0.8 to 0.9, Lt. eye from zero to 0.3. The visual field turbidity were absolutely cleared. The scotomas in Lt. eye were reduced from 5 to 2. The metamorphopsia were improved. The vitreous floaters in Rt. eye were decreased from 5~6 to 1~2. Conclusion : These results show that the oriental medical treatment can be conservative measure to the macular edema resulted from branch retinal vein occlusion.

• Journal of Veterinary Science
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• 제23권1호
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• pp.10.1-10.12
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• 2022

Background: Dry eye disease (DED) cannot be diagnosed by a single test because it is a multifactorial disorder of the ocular surface. Although studies on various dry eye tests (DETs) in dogs have been reported, standard criteria have not been established except for the Schirmer tear test 1 (STT-1). Objectives: To establish reference values for DETs in dogs with normal STT-1 values (≥ 15 mm/min) and to analyze the correlation between DETs. Methods: The STT-1 was performed in 10 healthy Beagle dogs (20 eyes). After 20 min, interferometry (IF) for estimating the lipid layer thickness (LLT) of the tear film, tear meniscus height (TMH), non-invasive tear breakup time (NIBUT), and meibomian gland loss rate of the upper eyelid (MGLRU) and lower eyelid (MGLRL), were measured using an ocular surface analyzer. Results: Mean values for STT-1, TMH, and NIBUT were as follows: 21.7 ± 3.4 mm/min, 0.41 ± 0.21 mm, and 19.1 ± 9.5 sec, respectively. The most frequent LLT range, measured by IF, was 30-80 nm (11 eyes), followed by 80 nm (5 eyes) and 80-140 nm (4 eyes). MGLRU ≤ 25% was measured in 11 eyes and 26%-50% in 9 eyes; MGLRL ≤ 25% in 8 eyes and 26%-50% in 12 eyes. Besides positive correlation between TMH and NIBUT (P = 0.038), there were no significant associations between DETs. Conclusions: Data obtained in this study provided normative references that could be useful for diagnosing DED and for further research into correlation between DETs in dogs with DED.

• 한방안이비인후피부과학회지
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• 제17권2호
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• pp.112-119
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• 2004

Objective: To carry out the oriental medical treatment on a patient with vitreous hemorrhage in the left eye caused by diabetic retinopathy and record the results of the treatment. Methods: 1. Diagnosis: Fundus photography, Colored paper, Dr. Hahn's standard test chart for 5M, Blood sugar measurement. 2. Treatment: Acupuncture, Electro-Acupuncture, Indirect moxibustion, Western medicines, Oryoungsan(Crude drug preparations) Results: Oriental treatment using Ohaeng-acupuncture, Electro-Acupuncture, Indirect moxibustion resulted in the Unaided visual acuity of 0.1 while it used to be the left eye visual acuity with only light sense I month ago. Looking from Fundus photography result, progress was achieved and diabetic retinopathy was found to be in progress in fluorescein fundus angiography to right eye also by revisiting the patient after treatment. Conclusions: 1. Vitrectomy has many advantages but there are instances where patients do not recover their visual acuity due to complications. Therefore it is necessary to prove the effect oriental medical treatment through more cases in future. 2 For diabetic retinopathy patients, diabetes must be treated together with visual acuity.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2004년도 하계학술대회 논문집 Vol.5 No.2
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• pp.1121-1124
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• 2004

Aging test to estimate life property of polymer insulator is executed through several international standard such as IEC 61109 and CEA tracking wheel test, but is not getting clear conclusion yet. There are two methods in the diagnosis method of polymer insulator such as off-line and on-line. The diagnosis methods in off-line are external condition analysis by the eye, contaminant analysis on surface, surface analysis, pollution withstand voltage test, power frequency flashover voltage test, lightning impulse flashover test, tensile fracture load test and flexural load test. The diagnosis methods in off-line most are the method for virgin and last aged sample. However, the diagnosis method in on-line is method that can be evaluate sample state as progressing continuously aging test in beginning, The diagnosis method in on-line is arranged as following: leakage current measurement, electric field, surface state investigation, thermal image, emitting light measurement and then so. In this paper, the tracking performance of polymer insulator with salt solution which is added surface active agent. The diagnosis of insulator sample has been analyzed by leakage current and visual examination.

• Journal of the Korean Wood Science and Technology
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• 제42권2호
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• pp.193-206
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• 2014

Residual performance of old architectural wood which has been damaged was measured using Nondestructive Evaluation (NDE). The wood Pole Tester was used to assess ultrasonic velocity inside wood and drill resistance was determined using an IML-resistograph. For ultrasonic measurements squared timber and circular timber's measurements were separately conducted with 1,300 m/s as the standard ultrasonic velocity. The standard wood samples divided into two parts; a non-sound area (below the standard), and a sound area (above the standard). Furthermore, schematization of wood was compared with results naked eye observation. The drilling resistance test was performed for both length and thickness direction in wood. The internal of the drilling was set at 30 cm (length direction), 5 cm (width direction) and 30cm (thickness direction). A non-sound area was defined as that 1) amplitude is below 20% and 2) carbonization and deterioration are related.

• 사상체질의학회지
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• 제26권4호
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• pp.350-364
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• 2014

Objectives This study was aimed to find the significant features of face form according to the Taeeumin and Soyangin by analyzing the three-dimensional face information data. Also, making standard face of the Taeeumin and Soyangin was an object of this study. Methods We collected three-dimensional face data of patients aged between 20~45 years old diagnosed by a specialist of Sasang constitutional medicine. The data were collected using a 3D scanner, Morpheus 3D(Morpheus Corporation, KOREA). Extracting a face feature point total of 64, was set to 332 pieces(height, angle, ratio, etc.) of each variable between feature points. ANOVA test were used to compare the characteristics of subjects according to the Taeeumin and Soyangin. Results When not to consider gender, the Taeeumin and Soyangin were different from the 18 items(3 items in the ear, 9 items in the eye, 1 item in the nose, 1 item in the mouth, 4 items in the jaw). When to consider gender, the Taeeumin and Soyangin men were different from the 6 items(1 item in the ear, 2 items in the nose, 3 items in the face). And the Taeeumin and Soyangin women were different from 17 items(1 item in the ear, 10 items in the eye, 2 items in the nose, 1 item in the mouth, 3 items in the face). Conclusions These results show Taeeumin's face(both men and women) width of the right and left is larger than the length of the top and bottom. Compared to men of Soyangin, men of Taeeumin has greater wings of the nose. Compared to women of Soyangin, women of Taeeumin has longer length of the eye. Soyangin's face(both men and women) length of the top and bottom is larger than the width of the right and left. Compared to men of Taeeumin, men of Soyangin has smaller wings of the nose. Compared to women of Taeeumin, women of Soyangin has more stereoscopic facial features at the top and bottom of the lateral face. Also, by accumulating three-dimensional face data, this study modeled the standard facial features by Taeeumin and Soyangin. These results may be helpful in the development of Sasang constitutional diagnostics utilizing the characteristics of the facial form at later.