• 한국응용과학기술학회지
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• 제40권6호
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• pp.1454-1463
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• 2023

Potentillae Chinensis Herba 추출물의 항산화 효능 평가를 위해 DPPH 자유라디칼 소거 활성과 ABTS 양이온 라디칼 활성 평가를 시행하였다. 세포 실험에서 항염증 평가를 위해 호흡기 점막 상피세포 NCI,H292와 RAW264.7 세포 대상으로 항염증 효능을 평가하였다. 그 결과, DPPH 자유라디칼 소거 활성과 ABTS 양이온 라디칼 활성 모두 농도 의존적으로 항산화 활성이 증가하는 것으로 나타났다. 세포 MTT 분석 결과, 각 농도 100 ㎍/ml로 처리한 경우 NCI-H292 세포의 생존율이 70% 미만으로 감소하였고, 이후 실험은 50 ㎍/ml로 진행하였다. 항염증 효능 평가에서는 NO생성, TNF-𝛼, IL-1𝛽, PGE₂가 감소하였고, COX-2도 50 ㎍/ml에서 유의하게 감소하였다. Potentillae Chinensis Herba 추출물과 생물전환 추출물의 뮤신 단백질 발현은 MUC5AC 발현이 유의하게 감소하는 것으로 관찰되었다. 이상의 결과는 Potentillae Chinensis Herba의 호흡기 점액 단백질 발현을 도와 염증을 억제하는 기능성 소재로 활용하는데 가치가 있음을 알 수 있다.

• Korean Journal of Acupuncture
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• 제40권3호
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• pp.62-78
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• 2023

Objectives : This study aims to assess the impact of acupotomy on migraine through an examination of clinical studies conducted since 2015. Methods : We conducted a comprehensive search for randomized controlled trials (RCTs) and non-randomized controlled trials (nRCTs) related to acupotomy treatment for migraine, utilizing five Korean online databases (OASIS, Science ON, DBPIA, KISS, RISS), as well as four foreign online databases (CNKI, PubMed, EMBASE, Cochrane Library). We identified a total of 10 relevant studies for analysis. Participants characteristics, treatment points, combination treatments, treatment cycles or frequencies, evaluation indices, efficacy, and adverse events were analyzed. The risk of bias in the 10 RCTs was assessed using the Revised Cochrane risk-of-bias tool for randomized trials (RoB 2.0). Results : A total of 931 participants were included in 10 studies. In the intervention group, the average duration of migraine morbidity ranged from 15.5±4.5 months to 15.9±4.2 years. Six studies based their diagnoses on the International Classification of Headache Disorders (ICHD), while five studies relied on Chinese diagnostic criteria. All studies specified the treatment area as the region exhibiting tenderness or induration on the head and neck. Treatment cycles ranged from a minimum of 2 days to a maximum of 1 week, with the number of days per treatment course varied from 5 days to 4 weeks. The diameter of acupuncture needles used varied between 0.3 mm and 1 mm. Of the eight studies specifying needle length, the shortest was 20 mm, and the longest was 40 mm. A total of eight evaluation indices were employed, with total efficacy rate (TER) and visual analogue scale (VAS) being the most frequently used. Statistically, all intervention groups showed more significant results compared to the control groups. Adverse events were reported in only two studies within the intervention group. Overall, the risk of bias assessment for the selected RCTs ranged from 'some concerns' to 'high risk of bias.' Conclusions : This study showed that acupotomy treatments for migraine were effective.

• 생명과학회지
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• 제27권2호
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• pp.225-232
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• 2017

견우자는 한의학에서 사하 작용 및 이뇨 작용 등의 치료 목적으로 사용되어 왔다. 본 연구에서는 견우자를 methanol로 추출하고(PNM), 이를 기본으로 ethyl acetate 분획물(PNE), butanol 분획물(PNB), water 분획물(PNW)로 나누었으며 각 분획물을 이용하여 mushroom tyrosinase 저해활성, pancreatic lipase 저해활성, DPPH free radical 소거활성 및 암세포주의 증식 저해활성 등을 조사하였다. 특히, 전립선암 세포주 PC-3 세포에서 강력한 세포 증식 저해활성을 보였으며, 용매 분획물 중, butanol 분획에서 가장 높은 암세포의 증식 저해활성을 보였다. 따라서 견우자의 methanol 추출물을 Diaion HP-20 column chromatography, reverse phase column chromatography, Sephadex LH-20 column chromatography를 순차적으로 실시하여 최종적으로 저해활성이 가장 높은 compound PN을 얻었으며 HPLC를 통해 그 순도를 확인하였다. Compound PN을 이용하여 cell cycle arrest과 annexin V/PI를 측정한 결과, 세포의 sub-G1기를 축적되면서 apoptosis가 유발되어 전립선암 세포주 PC-3 세포의 세포사멸이 유도되는 것을 확인하였다. 따라서 본 연구를 통하여 견우자에 대한 항산화 활성, 미백 활성 등의 효과 뿐 만 아니라 항암 활성에도 활용할 수 있을 것으로 사료된다.

• 동의생리학회지
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• 제14권2호통권20호
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• pp.67-82
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• 1999

The effects of Bogi, Boyang and Onri herbs on glutamte receptor, and the regulatory mechanism of cAMP-protein kinase on the ion currents activated by Bogi, Boyang and Onri herbs using nystatin-perforated patch clamp were investigated and the following results were obtained. Ginseng radix and Astragali radix, Cervi cornu and Boshniakiae herba, and Aconiti tuber and Zingiberis rhizoma were chosen as Bogi, Boyang and Onri herbs respectively. 1. The ion currents activated by $10^{-5}M$ of glycine were used as controls. The magnitudes of the ion currents by the above named herbs were as follows; Cervi cornu>Astragali radix>Aconiti tuber>Zingiberis rhizoma>Ginseng radix>Boshniakiae herba. 2. The magnitudes of the ion currents by $10^{-5}M$ of glutamate pre-treated with 0.01 mg/ml of Bogi, Boyang and Onri herbs were sharply decreased. 3. The activity of ion channels activated by Bogi herbs pre-treated with $10^{-7}M$ of staurosporin, an inhibitor of protein kinase, for thirty seconds was observed as the experiment proceeded. Staurosporin brought about dephosphorylation of ion channels. Hence, while the activity of ion channels activated by Ginseng radix was decreased, the activity of ion channels activated by Astragali radix was increased, as time went by. 4. The activity of ion channels activated by Boyang herbs pre-treated with $10^{-7}M$ of staurosporin, an inhibitor of protein kinase and an dephosphorylating agent of ion channels, for thirty seconds was investigated. While the activity of ion channels activated by Cervi cornu was increased, the activity of ion channels activated by Boshniakiae herba was initially increased then sharply decreased. 5, The activity of ion channels activated by Onri herbs pre-treated with $10^{-7}M$ of staurosporin, an inhibitor of protein kinase and an dephosphorylating agent of ion channels, for thirty seconds was investigated. While the activity of ion channels activated by Aconiti tuber was increased, that of ion channels activated by Zingiberis rhizomal sharply declined.

• 보건행정학회지
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• 제9권4호
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• pp.120-139
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• 1999

The objectives of this study were to examine the utilization patterns of oriental medical care and to discover problems in its delivery. The data for this study were collected from a questionnaire survey mailed out from March 10 to April 9 1999 to 6.346 oriental medical clinic. The questionnaires were then distributed to two patients in each clinics. Of these questionnaires. 670 were completed and returned. The major statistical methods used for the analysis were the t-test. ANOVA, and x$^2$-test. The major findings are as follows: 1. Respondents reported visiting oriental doctors twice as often as they visited western doctors(All those completing the survey received the questionnaire at oriental medical clinics). 2. The number of reported visits to oriental physicians according to among gender, age, marital status, education, income and residence. Males, married respondents, the elderly and the residents of rural areas visited oriental physicians more frequently than females, singles, younger respondents and urbanites. Those people belonging to the middle income class and middle education level also more frequently visited oriental physicians. 3. There are several factors that restrict the utilization of oriental medical care, such as the limitation of the scientific diagnostic instrument use commonplace reliance upon western medical techniques, and the perception of high price for oriental medical care. It is very important to focus oriental medical care onto the fields of acupuncture, circulatory system disease, musculoskeletal system ailments, etc. to improve the utilization of oriental medical care. Other policies for the improvement of oriental medical care include the standardization of price, quality and quantity of oriental medicine.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 추계학술대회
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• pp.159-160
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• 2021

우리 사회는 인공지능, 사물 인터넷, 자율주행, 공장 자동화 등 ICT 기술 기반의 4차 산업혁명과 함께 인구 고령화라는 큰 변화에 직면해있다. 더욱이 인구소멸위험 지수가 높은 지방자치단체에서는 고도화된 산업 자동화와 인구 고령화라는 변화에 대응하기 위해 지역 특성에 맞는 시장 확대 및 재교육 정책의 고려가 필수적이다. 고령자 재취업을 위해 노인 친화형 일자리를 발굴하고 고령친화 산업에 대한 투자 확대, 고령자를 많이 고용하는 기업의 작업환경 개선 정책에 대해 알아보고, 천안의 노인 노동시장의 실태와 개선방향에 중점을 두고 해결책을 모색하고자 한다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 추계학술대회
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• pp.170-171
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• 2021

전 세계의 많은 전문가들은 코로나19 바이러스가 완전히 종식된 이후에도 코로나 이전과 똑같은 모습으로 돌아가기 어려울 것이라고 예측한다. 코로나19 사태의 장기화로 비대면 추세가 지속되면서 스포츠 활동이 위축되고 국민들의 운동량은 감소하였다. 사회적 거리두기 단계 상향으로 다중이용시설 이용이 점차 제한되자 비대면 서비스에 대한 수요가 증가하고 있다. 본 연구는 생활체육 참여 실태를 파악하여 포스트 코로나 시대에 적합한 생활체육 활성화방안에 대해서 고찰한다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 추계학술대회
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• pp.147-149
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• 2021

세종특별자치시의 교통량 폭증으로 인한 교통 문제 증가 추세는 시설 인프라의 투자로는 해결하기 어려운 수준에 이르러 데이터 기반의 지능형 교통환경 구축이 필수적이다. 교통데이터를 활용해 교통안전정책을 수립한 국내·외 사례를 알아보고 세종특별자치시 교통정보 및 데이터 활용 실태 등을 분석하여 CCTV를 활용한 주차정보 제공, 스마트 교통신호제어시스템 구축, 안전지키미 드롭존 설치방안을 제안한다. 본 연구가 향후 세종특별자치시의 교통 안전망 강화 정책 수립의 기반이 되기를 기대한다.

• 한국식품영양학회지
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• 제34권5호
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• pp.498-505
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• 2021

This study tried to develop Jinmal dasik using pine nuts leaf powder with antibacterial and antioxidant properties. After preparing the Jinmal dasik in proportions of 0%, 2%, 4%, 6%, and 8% by varying the ratio of pine needle powder, the moisture, color, physical properties, antioxidant properties and sensory quality were measured. Except for the 8% Jinmal dasik, the L value of chromaticity significantly decreased as the amount of pine needle powder increased (p<0.001). In addition, the value significantly decreased with increased density (p<0.001). Based on our physical properties evaluation results, the hardness, cohesiveness, and elasticity decreased significantly as the amount of pine needle powder increased (p<0.001), but adhesion and chewiness increased significantly with an increase in the amount of pine needle powder (p<0.001). Total polyphenol content was significantly increased except for the 8% Jinmal dasik (p<0.001). DPPH and ABTS also increased significantly as the amount of pine needle powder increased (p<0.001). Our sensory evaluation, showed the 2% Jinmal dasik to have the highest degree of preference, however, there was no significant difference among the proportion groups (p=0.053).

• 대한예방한의학회지
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• 제3권1호
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• pp.55-65
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• 1999

From January 1994 to April 1997, there was 15 survivals who was admitted to the department of internal medicine, Wonkwang Oriental Medicine Hospital in Cheun-ju, after ingestion of paraquat, and treated with Oriental and western medicine therapy. For the patients, I investigated clinical symptoms, gastroduodenoscopy, intake by oral and parenteral route, and output by urine and stool, serum ALP, AST. ALT, Bilirubin, BUN, Creatinine level and urine analysis. On admission day, the LFT level was as follows. The serum mean ALP, AST, ALT, Total Bilirubin and Direct Bilirubin was $10.05{\pm}2.75\;KAU$, $66.67{\pm}9.88\;IU／L$, $43.80{\pm}7.74\;IU／L$, $1.89{\pm}1.22\;mg／dl$ and $1.10{\pm}1.14\;mg／dl$ respectively. After that day, administered Gamdutang and checked the mean LFT level regullary. Until the 3rd day, the mean ALP, AST, ALT, Total Bilirubin and Direct Bilirubin level was $11.01{\pm}3.16\;KAU$, $56.47{\pm}7.19\;IU／L$, $59.00{\pm}7.57\;IU／L$, $2.54{\pm}1.78\;mg／dl$, $1.64{\pm}1.59\;mg／dl$ respectively. From 4th day to 7th day, the mean ALP; AST, ALT, Total Bilirubin and Direct Bilirubin level was $12.51{\pm}3.49\;KAU$, $77.85{\pm}7.17\;IU／L$, $58.00{\pm}9.09\;IU／L$, $2.54{\pm}1.97\;mg／dl$, and $1.80{\pm}1.81\;mg／dl$ respectively. From 8th day to 10th day, the mean ALP, AST, ALT, Total Bilirubin and Direct Bilirubin level was $12.43{\pm}3.14\;KAU$, $41.13{\pm}6.49\;IU／L$, $50.40{\pm}7.17\;IU／L$, $1.66{\pm}1.90\;mg／dl$ and $1.14{\pm}1.50\;mg／dl$ respectively. From 11th day to 14th day, the mean ALP, AST, ALT, Total Bilirubin and Direct Bilirubin level was $12.30{\pm}3.25\;KAU$, $31.07{\pm}3.85\;IU／L$, $43.33{\pm}5.49\;IU／L$, $1.62{\pm}1.95\;mg／dl$, $1.17{\pm}1.71\;mg／dl$ respectvely. On admission day, the mean RFT level as follows. Serum BUN and Creatinine level was $28.73{\pm}5.19\;mg／dl$ and $1.82{\pm}1.27\;mg／dl$ respectively. After that day, administered Gamdutang and checked the mean RFT level regullary. Until the 3rd day, the mean BUN and Creatinine level was $32.12{\pm}5.65\;mg／dl$ and $2.31{\pm}0.45\;mg／dl$ respectively. From 4th day to 7th day, the mean BUN and Creatinine level was $31.07{\pm}5.47\;mg／dl$ and $1.92{\pm}0.79\;mg／dl$ respectively. From 7th day to 10th day, the mean BUN and Creatinine level was $17.47{\pm}3.57\;mg／dl$ and $1.33{\pm}0.59\;mg／dl$ respectively. From 11th day to 14th day, the mean BUN and Creatinine level was $11.93{\pm}3.16\;mg／dl$, $1.27{\pm}0.38\;mg／dl$ respectively.