• Journal of Life Science
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• v.23 no.2
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• pp.167-174
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• 2013

Peroxiredoxin 6 (Prx 6) is a member of the thiol-specific antioxidant protein family, which may play a role in protection against oxidative stress and in regulating phospholipid turnover. The aim of this study was to determine whether a human Prx 6/Luc vector was stably expressed and responded to antioxidants in a lung cell line (NCI-H460). To achieve this, the luciferase signal, hPrx 6 mRNA expression, and superoxide dismutase (SOD) activity were measured in transfectants with a hPrx 6/Luc plasmid after treatment with four antioxidant extracts, including Korea white ginseng (KWG), Korea red ginseng (KRG), Liriope platyphylla (LP), and red Liriope platyphylla (RLP). First, the hPrx 6/Luc plasmid was successfully constructed with DNA fragments of human Prx 6 promoter, amplified by PCR using genomic DNA isolated from NCI-H460 cells, and cloned into the pTransLucent reporter vector. The orientation and sequencing of the hPrx 6/Luc plasmid were identified with restriction enzyme and automatic sequencing. A luciferase assay revealed significant enhancement of luciferase activity in the four treatment groups compared with a vehicle-treated group, although the ratio of the increase was different within each group. The KRG- and LP-treated groups showed higher activity than the KWG- and RLP-treated groups. Furthermore, the luciferase activity against RLP occurred roughly in a dose-dependent manner. However, the level of endogenous hPrx 6 mRNA did not change in any group treated with the four extracts. The SOD activity was in agreement with the luciferase activity. Therefore, these results indicate that the hPrx 6/Luc vector system may successfully express and respond to antioxidant compounds in NCI-H460 cells. The data also suggest that the Prx 6/Luc vector system may be effectively applied in screening the response of hPrx 6 to antioxidant compounds in transgenic mice.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.22 no.3
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• pp.118-134
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• 2017

The radiation observation data will be used importantly in research field such as climatology, weather, architecture, agro-livestock and marine science. The Ieodo Ocean Research Station (IORS) is regarded as an ideal observatory because its location can minimize the solar radiation reflection from the surrounding background and also the data produced here can serve as a reference data for radiation observation. This station has the potential to emerge as a significant observatory and join a global radiation observation group such as the Baseline Surface Radiation Network (BSRN), if the surrounding of observatory is improved and be equipped with the essential radiation measuring instruments (pyaranometer and pyrheliometer). IORS has observed the solar radiation using a pyranometer since November 2004 and the data from January 1, 2005 to December 31, 2015 were analyzed in this study. During the period of this study, the daily mean solar radiation observed from IORS decreased to $-3.80W/m^2/year$ due to the variation of the sensor response in addition to the natural environment. Since the yellow sand and fine dust from China are of great interest to scientists around the world, it is necessary to establish a basis of global joint response through the radiation data obtained at the Ieodo as well as at Sinan Gageocho and Ongjin Socheongcho Ocean Research Station. So it is an urgent need to improve the observatory surrounding and the accuracy of the observed data.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.11 no.3
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• pp.229-234
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• 2001

The EDXRF(Energy Dispersive X-ray Fluorescence Spectrometer) technique was applied to the determination of heavy metals in welding fume. The EDXRF method designed in this study was a non-destructive analysis method. Samples were analyzed directly by EDXRF without any pre-treatment such as digestion and dilution. The samples used to evaluate this method were laboratory samples exposed in a chamber connected with a welding fume generator. The samples were first analyzed using a non-destructive EDXRF method. The samples subsequently were analyzed using AAS method to verify accuray of the EDXRF method. The purpose of this study was to evaluate the possibility of the non-destructive analysis of heavy metals in welding fume by EDXRF. The results of this study were as follow: 1.When the samples were collected under the open-face sampling condition, a surface distribution of welding fume particles on sample filters was uniform, which made non-destructive analysis possible. 2. The method was statistically evaluated according to the NIOSH(National Institute for Occupational Safety and Health) and HSE(Health and Safety Executive) method. 3. The overall precision of the EDXRF method Was calculated at 3.45 % for Cr, 2.57 % for Fe and 3.78 % for Mn as relative standard deviation(RSD), respectively. The limits of detection were calculated at $0.46{\mu}g$/sample for Cr, $0.20{\mu}g$/sample for Fe and $1.14{\mu}g$/sample for Mn, respectively. 4. A comparison between the results of Cr, Fe, Mn analyzed by EDXRF and AAS was made in order to assess the accuracy of EDXRF method. The correlation coefficient between the results of EDXRF and AAS was 0.9985 for Cr, 0.9995 for Fe and 0.9982 for Mn, respectively. The overall uncertainty was determined to be ${\pm}12.31%$, 8.64 % and 11.91 % for Cr, Fe and Mn, respectively. In conclusion, this study showed that Cr, Fe, Mn in welding fume were successfully analyzed by the EDXRF without any sample pre-treatment such as digestion and dilution and a good correlation between the results of EDXRF and AAS was obtained. It was thus possible to use the EDXRF technique as an analysis method of working environment samples. The EDXRF method was an efficient method in a non-destructive analysis of heavy metals in welding fume.

• Journal of Food Hygiene and Safety
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• v.27 no.4
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• pp.366-374
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• 2012

Recently pathogenic E. coli is one of the main foodborne pathogens resulting in many patients in Korea. To understand the characteristics of pathogenic E. coli outbreaks in Korea, the epidemiological investigation reports of pathogenic E. coli outbreak in 2009 (41 reports) and in 2010 (27 reports) were collected in the web site of the Korea Centers for Disease Control and Prevention, reviewed and analysed in this study. The main places of the pathogenic E. coli outbreaks were food catering service area (64.8%) and restaurants (25.0%). The main type of the pathogens were EPEC (44.7%) and ETEC (34.2%). EAEC and EHEC was responsible for 10.5 and 9.2%, respectively. Eight of 68 outbreak cases were caused by more than 2 types of pathogenic E. coli which implicates the complicated contamination pathways of pathogenic E. coli. The incidence rate of pathogenic E. coli was $33.6{\pm}30.5%$ and the main symptoms were diarrhea, stomach ache, nausea, vomiting, and fever etc. The two identified food sources were identified as frozen hamburger pattie and squid-vegetable mixture. To improve the food source identification by epidemiological investigation, food poisoning notification to the agency should not be delayed, whole food items attributed the outbreak should be collected and detection method of the various pathogenic E. coli in food has to be improved. In conclusion, the characteristics between the EHEC outbreaks in the western countries and the EPEC or ETEC outbreaks in Korea needs to be distinguished to prepare food safety management plan. In addition, the development of the trace back system to find the contamination pathway with the improved detection method in food and systemic and cooperative support by the related agencies are necessary.

• Journal of Food Hygiene and Safety
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• v.33 no.2
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• pp.140-145
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• 2018

The skin of the human body occupies the largest surface area of the body and acts as a protection for the person's internal organs. As such, the skin is a major target of oxidative stressors, and these oxidative stressors are known to contribute to skin aging over the course of time. For the most part, an antioxidant is an effective approach to utilize to prevent symptoms related to the reactive oxygen species (ROS)-induced aging of the skin. Therefore, we investigated the antioxidant and anti-aging activity of the leaves of the Quercusaliena Blume extract (QBE). In our study, we confirmed that the cell viability tested with XTT {2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide innersalt} assay was not affected up to a concentration of $100{\mu}g/mL$. In addition, the cell viability of HDF cells induced by hydrogen peroxide was recovered from 81% to 104% after treatment with QBE, which showed the greater protective effect than that of ascorbic acid. Treatments of QBE dose-dependently inhibited reactive oxygen species (ROS) production in HDF cells induced by hydrogen peroxide, which correlated with their protective effects on cell viability. Since QBE treatment exhibited the suppression effect of skin aging by decreasing the ROS production, QBE could be used as a not only natural anti-aging but also antioxidant resource.

• Restorative Dentistry and Endodontics
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• v.35 no.6
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• pp.453-460
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• 2010

Objectives: To evaluate the accuracy and consistency of two different apex locators at both the Apex and 0.5 marks. Materials and Methods: Twenty-six root canals was scheduled for extraction for periodontal or prosthodontic reasons. Thirteen canals were measured using Root ZX and the rest by i-ROOT. The root canal length was measured both the at 0.5 mark and the Apex mark. The file was then fixed to the toot, and the distance from the file tip to the major foramen of each canal was measured after removing the root dentin under the microscope so that the major foramen and the file tip were seen. Results: 1. When the Apex mark was used, 100% of both the Root ZX and i-ROOT groups were within 0.5 mm of the major foramen. 2. When 0.5 mark was used, 100% of the Root ZX group and 77% of the i-ROOT group were within 0.5 mm of the major foramen. 3. In terms of standard deviation and quartile value, the Apex mark was more consistent than 0.5 mark in the Root ZX group, and 0.5 mark was more consistent in the i-ROOT group, but there was no statistically significant difference when compared with t-test. 4. The root canal length difference between the Apex mark and 0.5 mark was 0.22 mm and 0.46 mm in the Root ZX and i-ROOT groups, respectively. Conclusions: In this study, the Apex mark was the more consistent mark. Therefore, it is recommended to subtract 0.5 mm, which is the average length between the apex and apical constriction, from the root canal length at the Apex mark to obtain the working length clinically.

• Ultrasonography
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• v.32 no.2
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• pp.132-142
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• 2013

Purpose: The purpose of this study is to investigate the correlations of various kinetic parameters derived from the time intensity curve in a xenograft mouse model injected with a prostate cancer model (PC-3 and LNCaP) using an ultrasound contrast agent with histopathologic parameters. Materials and Methods: Twenty nude mice were injected with human prostate cancer cells (15 PC-3 and five LNCaP) on their hind limbs. A bolus of $500{\mu}L$ ($1{\times}10^8$ microbubbles) of second-generation US contrast agent (SonoVue) was injected into the retroorbital vein. The region of interest was drawn over the entire tumor. The time intensity curve was acquired and then fitted to a gamma variate function. The maximal intensity (A), time to peak (Tp), maximal wash-in rate (washin), washout rate (washout), area under the curve up to 50 sec ($AUC_{50}$), area under the ascending slope ($AUC_{in}$), and area under the descending slope ($AUC_{out}$) were derived from the parameters of the gamma variate fit. Immunohistochemical staining for VEGF and CD31 was performed. Tumor volume, the area percentage of VEGF stained in a field, and the count of CD31 (microvessel density, MVD) positive vessels showed correlation with the parameters from the time intensity curve. Results: No significant differences were observed between the kinetic and histopathological parameters from each group. MVD showed positive correlation with A (r=0.625, p=0.003), washin (r=0.462, p=0.040), $AUC_{50}$ (r=0.604, p=0.005), and $AUC_{out}$ (r=0.587, p=0.007). Positive correlations were also observed between tumor volume and $AUC_{50}$ (r=0.481, p=0.032), washin (r=0.662, p=0.001), and $AUC_{out}$ (r=0.547, p=0.012). Washout showed negative correlations with MVD (r=-0.454, p=0.044) and tumor volume (r=-0.464, p=0.039). The area percentage of VEGF did not show any correlation with calculated data from the curve. Conclusion: MVD showed correlations with several of the kinetic parameters. CEUS has the potential for prediction of tumor vascularity in a prostate cancer animal model.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.606-610
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• 2011

'Jungmo1001' is an early maturing intermediate breeding line developed from a cross between Cheolweon52 and SR14694-57-4-2-1-3-2-2 by the rice breeding team of National Institute of Crop Science, Rural Development Administration (RDA) in 1994. 'Jungmo1001' has about 107 days duration from seeding to heading in mid-northern inland plain, southern alpine area and north-eastern coastal areas. It has about 73 cm in culm length with semi-erect plant type and good canopy architecture. This variety has 13 tillers per hill and 90 spikelets per panicle. Its 1,000 grain-weight of brown rice is 21.2 g which is less than 26.3 g of 'Odaebyeo'. Milled kernels are translucent with non-glutinous endosperm, low amylose content (18.1%) compared with 'Odaebyeo' palatability of cooked rice is very good. This variety shows strong resistance to cold treatment, lodging, premature heading and wilting. This variety shows moderately resistant to blast disease but susceptible to bacterial blight, stripe virus and insect pests. The milled rice yield performance of this variety is about 5.45 MT/ha by ordinary culture in local adaptability test for three years. This variety may be highly adaptable to the mid-northern inland plain, southern alpine area and north-eastern coastal areas of Korea.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.3
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• pp.119-126
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• 2003

Nitrate-N concentrations and the corresponding ${\delta}^{15}N$ values were determined with water samples collected periodically from artesian wells (3 and 6 m deep), underdrainage and gushout waters in a Welsh onion cultivated area in the Kushibiki Fan, Saitama Prefecture, Japan. Average $NO_3-N$ concentrations in waters from 3 and 6 m wells were 25.7 and $2.8mg\;L^{-1}$, whereas ${\delta}^{15}N$ values were 3.6 and 4.7‰, respectively. The $NO_3-N$ concentration and ${\delta}^{15}N$ value of the underdrainge water were $35.5mg\;L^{-1}$ and 6.6‰, reflecting rapid input of chemical fertilizers and farmyard manure. The mean values of $NO_3-N$ concentration and ${\delta}^{15}N$ in the gushout water flown out of the edge of Kushibiki Fan were $19.4mg\;L^{-1}$ and 7.9‰, respectively. As a results the ${\delta}^{15}N$ values of the gushout water were higher than those of the artesian wells and underdrinage water. The ${\delta}^{15}N$ values of total-N and $NO_3-N$ of the soils were 6.1 and 5.10‰, respectively, while those for nitrification-inhibitor containing fertilizer and slow-release fertilizers were -6.1 and -2.2‰, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.3
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• pp.353-360
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• 2011

In order to develop the application method of pig compost (PC) and liquid manure (LM) for whole crop barley cultivation, experiments were conducted at Munpo series (coarse loamy, mixed, nonacid, mesic family of Typic Fluvaquents) soil in Gyehwa-reclaimed land, six plots, a LM applied rate as N% ; non-application, chemical fertilizer (CF)100, 100, 50+50, 50+CF50 and (PC30+LM40)+LM50 as basal and additional fertilizer. $NO_3^-$-N content in soil was decreased as along with the growth of plant, highest in LM100% as basal fertilization at early growth stage and highest in (PC30%+LM40%)+LM40% and CF100% at last growth stage. Amount of $NO_3^-$-N and $NH_4^+$-N in soil was high in (PC30%+LM40%)+LM40% and CF100% of top soil but in subsoil significant difference was little in all treatment. Amount of OM, $A_V.P_2O_5$, T-N, exchangeable Ca and Na in soil was higher (PC30%+LM40%)+LM40% than non-application after harvest. Amount of nutrient uptake in plant was higher in CF100% and split application of LM than LM 100% application. Nitrogen utilization rate was in the order of CF100% >LM50%+LM50%=LM50%+CF50%>(PC30%+LM40%)+LM40% >LM100%. The yield of whole crop barley in (PC30%+LM40%)+LM40% and CF100% was 3.2 times more than in non-application ($309kg\;10a^{-1}$). Feed values such as crude protein and TDN was increased 1.0% ~ 1.4% in LM as split application than basal 100% treatment. Accordingly, in order to increase yield of a whole crop barley with application PC+LM in reclaimed land treat split application rather than to treat LM 100% into the land.