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http://dx.doi.org/10.13103/JFHS.2018.33.2.140

Antioxidant and Anti-aging Effects of Extracts from Leaves of the Quercusaliena Blume on Human Dermal Fibroblast  

Choi, Sun-Il (Department of Food Science and Biotechnology, Kangwon National University)
Lee, Jong Seok (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources)
Lee, Sarah (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources)
Yeo, Joohong (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources)
Jung, Tae-Dong (Department of Food Science and Biotechnology, Kangwon National University)
Cho, Bong-Yeon (Department of Food Science and Biotechnology, Kangwon National University)
Choi, Seung-Hyun (Department of Food Science and Biotechnology, Kangwon National University)
Sim, Wan-Sup (Department of Food Science and Biotechnology, Kangwon National University)
Han, Xionggao (Department of Food Science and Biotechnology, Kangwon National University)
Lee, Jin-Ha (Department of Food Science and Biotechnology, Kangwon National University)
Kim, Jong Dai (Department of Food Science and Biotechnology, Kangwon National University)
Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
Publication Information
Journal of Food Hygiene and Safety / v.33, no.2, 2018 , pp. 140-145 More about this Journal
Abstract
The skin of the human body occupies the largest surface area of the body and acts as a protection for the person's internal organs. As such, the skin is a major target of oxidative stressors, and these oxidative stressors are known to contribute to skin aging over the course of time. For the most part, an antioxidant is an effective approach to utilize to prevent symptoms related to the reactive oxygen species (ROS)-induced aging of the skin. Therefore, we investigated the antioxidant and anti-aging activity of the leaves of the Quercusaliena Blume extract (QBE). In our study, we confirmed that the cell viability tested with XTT {2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide innersalt} assay was not affected up to a concentration of $100{\mu}g/mL$. In addition, the cell viability of HDF cells induced by hydrogen peroxide was recovered from 81% to 104% after treatment with QBE, which showed the greater protective effect than that of ascorbic acid. Treatments of QBE dose-dependently inhibited reactive oxygen species (ROS) production in HDF cells induced by hydrogen peroxide, which correlated with their protective effects on cell viability. Since QBE treatment exhibited the suppression effect of skin aging by decreasing the ROS production, QBE could be used as a not only natural anti-aging but also antioxidant resource.
Keywords
Rreactive oxygen species; Human dermal fibroblast; Antioxidant; Anti-aging;
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