• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.297-312
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• 2014

Food safety is increasingly becoming an important public health issue, as foodborne diseases present a widespread and growing public health problem in both developed and developing countries. The rapid and precise monitoring and detection of foodborne pathogens are some of the most effective ways to control and prevent human foodborne infections. Traditional microbiological detection and identification methods for foodborne pathogens are well known to be time consuming and laborious as they are increasingly being perceived as insufficient to meet the demands of rapid food testing. Recently, various kinds of rapid detection, identification, and monitoring methods have been developed for foodborne pathogens, including nucleic-acid-based methods, immunological methods, and biosensor-based methods, etc. This article reviews the principles, characteristics, and applications of recent rapid detection methods for foodborne pathogens.

• Computers and Concrete
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• v.25 no.2
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• pp.145-154
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• 2020

For the influence of the propagation law of stress wave at the coal-rock interface during the pre-blasting of the top coal in top coal mining, the ANSYS-LS/DYNA fluid-solid coupling algorithm was used to numerical calculation and the life-death element method was used to simulate the propagation of explosion cracks. The equation of the crushing zone and the fracturing zone were derived. The results were calculated and showed that the crushing radius is 14.6 cm and the fracturing radius is 35.8 cm. With the increase of the angles between the borehole and the coal-rock interface, the vibration velocity of the coal particles and the rock particles at the interface decreases gradually, and the transmission coefficient of the stress wave from the coal mass into the rock mass decreases gradually. When the angle between the borehole and the coal-rock interface is 0°, the overall crushing degree is about 11% and up to the largest. With the increase of the distance from the charge to the coal-rock interface, the stress wave transmission coefficient and the crushing degree of the coal-rock are gradually decreased. At the distance of 50 cm, the crushing degree of the coal-rock reached the maximum of approximately 12.3%.

• Earthquakes and Structures
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• v.16 no.1
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• pp.109-118
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• 2019

This study, analyses the seismic response for a rigid massless square foundation resting on a viscoelastic soil layer limited by rigid bedrock. The foundation is subjected either to externally applied forces or to obliquely incident seismic body or surface harmonic seismic waves P, SV and SH. A 3-D frequency domain BEM formulation in conjunction with the thin layer method (TLM) is adapted here for the solution of elastodynamic problems and used for obtained the seismic response. The mathematical approach is based on the method of integral equations in the frequency domain using the formalism of Green's functions (Kausel and Peck 1982) for layered soil, the impedance functions are calculated by the compatibility condition. In this study, The key step is the characterization of the soil-foundation interaction with the input motion matrix. For each frequency the impedance matrix connects the applied forces to the resulting displacement, and the input motion matrix connects the displacement vector of the foundation to amplitudes of the free field motion. This approach has been applied to analyze the effect of soil-structure interaction on the seismic response of the foundation resting on a viscoelastic soil layer limited by rigid bedrock.

• Steel and Composite Structures
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• v.38 no.1
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• pp.79-86
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• 2021

Due to the complex geological conditions, a large number of high quality coal seams was buried in the western of China which cannot be mining in open-pit methods. The dynamic properties of that coal cannot be studied easily in real site for the complex working condition. The compound coal blocks made on the basis of the real situation were studied in the laboratory. The physical and mechanical properties of the compound coal blocks and the raw coal were contrasted by using the UCS tests. The results show that the compound coal blocks made by mixing coal powder, cement and water in proportion of 2.5:2:1 are the closest to that of standard raw coal. Then the propagation of strain waves and crushing effects on the coal were studied in the compound coal blocks by using the super dynamic strain test system and the numerical calculated method of ANSYS/LS-DYNA. The results show that the diameter of the crushing zone in the compound coal blocks was similar to that in the numerical results. The fractures distribution in laboratory tests also has a similar trend to the calculation results. The measured strain waves at the distance of 50 cm, 100 cm, and 150 cm from the center of the charge are mainly concerned at -1.0×104 με and have a similar trend as that in the numerical simulation.

• Journal of Information Technology Applications and Management
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• v.30 no.6
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• pp.81-90
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• 2023

In aviation, with the rapid transformation of the mobility industry, UAMs are emerging to operate green low-altitude airspace in urban environments. In order for UAM aircraft to fly safely transporting passengers and cargo in low-altitude urban airspace, a traffic control system that supports the safe operation of the aircraft is essential. In particular, traffic control systems that reflect the characteristics of the flight environment, such as operating at low altitude in urban environments for a short period of time, are required. In this study, we define the definition of UATM and its main services that perform traffic control for the safe operation of UAMs. In addition, we analyzed the development trends of UATM systems based on domestic and overseas cases. Based on these analyses, we present the results of the concept design of the UATM system. After analyzing UATM development cases, we found that there is no commercialized UATM system, but overseas development is focused on systems that can integrate ATM and UTM. And we identified key stakeholders and interface data, and performed UATM system architecture and functional design based on the identified data. Finally, as a necessary element for the future development of UATM systems, we propose the establishment and advancement of UAM traffic flow management systems, the establishment of integrated control systems, and the development of interface with aircraft operation systems in preparation for the unmanned UAM aircraft.

• Membrane Journal
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• v.26 no.6
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• pp.407-420
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• 2016

The fuel cell technology as a green energy source has been actively studied to solve energy shortages and pollution problems. The generating efficiency of fuel cell is high because the electricity is directly produced by using hydrogen and oxygen and the additional power generator is not needed. The key technology is the manufacturing process of polymer electrolyte membranes for polymer electrolyte membrane fuel cell (PEMFC) system. The Nafion, perfluoro-based polymeric membrane is mainly used as a polymer electrolyte membrane. However, the Nafion is expensive and rapidly decreases the performance of Nafion at high temperature. So, many researchers are lively studying new alternative electrolyte membranes. In this review, through the technology competitiveness evaluation of patents and papers, the frequencies of presentation are filed by country, institution and company. In addition, polymer electrolyte membrane fuel cell, direct methanol fuel cell and alkaline fuel cell are also filed.

• Membrane Journal
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• v.23 no.3
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• pp.189-203
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• 2013

F-gases, gases containing fluorine such as perfluorocarbons (PFCs), sulfurhexafluoride ($SF_6$), nitrogen trifluoride ($NF_3$) are known to have green house effects. Although the net emission rates of gases containing fluorine are much lower than those of $CO_2$, their contribution to global warming cannot be ignored because of their extremely high global warming potential (GWP). F-gases mainly have been used for a variaty of applications in the semiconductor/LCD processes and in the electric power distribution industry of the national key industry. One of practical solutions of controlling the emission rates of F-gases is to reuse by separation and recovery of F-gases of low concentration from the gases mixtures with nitrogen or air. This work investigates some methods for F-gases recovery and separation around the membrane-based process.

• Research in Plant Disease
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• v.14 no.2
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• pp.138-141
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• 2008

Cucumber is high valued cash crop, for it is grown during the winter season in plastic house. Recently, virus disease spread widely in cucumber growing area and cause severe income loss. Therefore, occurrence of virus disease on cucumber were surveyed from 2004 to 2006 in Sangju and Gunwi area, Gyeongbuk province. The rate of plastic house which has infected plants was $55.0{\sim}88.6%$. Infection rate was the highest at Sangju in 2006 than others and ranged from 15 to 90.0% per plastic house. The 217 samples showing virus symptom were analyzed by RT-PCR using appropriate detection primer. Zucchini yellow mosaic virus(ZYMV) has the highest infection rate(detected over 85%) and followed by Cucumber green mottle mosaic virus(CGMMV). But Watermelon mosaic virus-2(WMV-2) was not detected in our survey. Therefore, we conclude that ZYMV is major pathogene of virus disease on cucumber. ZYMV induced chlorosis and severe mosaic on the leaves and distortion on the surface of fruits.

• Transactions of the Korean hydrogen and new energy society
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• v.21 no.5
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• pp.435-441
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• 2010

To verify the possibility of a power generation system using biogas from the waste of pig farm for rural electric production, a SI gasoline engine is modified to use biogas fuel and was installed in a 20 KVA power generation system. An electronic speed regulation unit is developed to keep the system speed at 1500 rpm. Experimental investigations have been carried out to examine the performance characteristics of power generation system (such as: system frequency, phase output voltage,$\ldots$). In addition, the operating parameters and output emissions ($NO_x$, HC, and $CO_2$) of biogas-fueled engine are preliminary evaluated and analyzed for the change of system load. Results indicated that the researched power generation system shows a high stability of output voltage and frequency with help of speed regulator. Biogas fuel (mainly $CH_4$ and $CO_2$) has an environmental impact and potential as a green alternative fuel for SI engine and they would not require significant modification of existing engine hardware. Output emissions of biogas-fueled engine are found to be relative low. $NO_x$ emission increases with the increase of output electric power of the power generation system.

• Journal of Veterinary Science
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• v.22 no.5
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• pp.73.1-73.11
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• 2021

Background: Feline calicivirus (FCV) is a common pathogen of felids, and FCV vaccination is regularly practiced. The genetic variability and antigenic diversity of FCV hinder the effective control and prevention of infection by vaccination. Improved knowledge of the epidemiological characteristics of FCV should assist in the development of more effective vaccines. Objectives: This study aims to determine the prevalence of FCV in a population of cats with FCV-suspected clinical signs in Hangzhou and to demonstrate the antigenic and genetic relationships between vaccine status and representative isolated FCV strains. Methods: Cats (n = 516) from Hangzhou were investigated between 2018 and 2020. The association between risk factors and FCV infection was assessed. Phylogenetic analyses based on a capsid coding sequence were performed to identify the genetic relationships between strains. In vitro virus neutralization tests were used to assess antibody levels against isolated FCV strains in client-owned cats. Results: The FCV-positive rate of the examined cats was 43.0%. Risk factors significantly associated with FCV infection were vaccination status and oral symptoms. Phylogenetic analysis revealed a radial phylogeny with no evidence of temporal or countrywide clusters. There was a significant difference in the distribution of serum antibody titers between vaccinated and unvaccinated cats. Conclusions: This study revealed a high prevalence and genetic diversity of FCV in Hangzhou. The results indicate that the efficacy of FCV vaccination is unsatisfactory. More comprehensive and refined vaccination protocols are an urgent and unmet need.