• Journal of Microbiology and Biotechnology
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• 제33권2호
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• pp.151-166
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• 2023

Yeast extract is a product prepared mainly from waste brewer's yeast, which is rich in nucleotides, proteins, amino acids, sugars and a variety of trace elements, and has the advantages of low production cost and abundant supply of raw material. Consequently, yeast extracts are widely used in various fields as animal feed additives, food flavoring agents and additives, cosmetic supplements, and microbial fermentation media; however, their full potential has not yet been realized. To improve understanding of current research knowledge, this review summarizes the ingredients, production technology, and applications of yeast extracts, and discusses the relationship between their properties and applications. Developmental trends and future prospects of yeast extract are also previewed, with the aim of providing a theoretical basis for the development and expansion of future applications.

• Korean Journal of Chemical Engineering
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• 제35권12호
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• pp.2487-2495
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• 2018

Inspired by the specific amino acid sequence Asn-Pro-Ala (NPA) of water channel aquaporins (AQPs), we fabricated polyamide (PA) nanofiltration (NF) membranes by introducing reduced glutathione (GSH) in interfacial polymerization (IP) method. Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectrometry (XPS), scanning electron microscope (SEM), atomic force microscopy (AFM), zeta potential and static water contact angle measurement were employed to characterize the chemical composition, morphology, electronegativity and hydrophilicity of the NF membranes. The water flux of GSH/PIP-TMC NF membrane reached $32.00L\;m^{-2}h^{-1}$ at 0.2 MPa, which was approximately twice than that of pristine PIP-TMC NF membrane when the ratio of GHS to piperazidine (PIP) was 40% during IP process. More water channels were built as GSH was embedded into PA layer. The fabricated NF membranes also took on potent rejection for dyes and $Na_2SO_4$. This study presents a simple and facile method to simulate water channels-based biological materials which may find potential application in water treatment.

• 기술혁신학회지
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• 제16권2호
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• pp.476-505
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• 2013

우리나라 정부는 '저탄소 녹색성장'을 새로운 국가발전 패러다임으로 제시한 이래로 '녹색기술 연구개발 종합대책('09.1)'을 통해 27대 중점 녹색기술을 도출하는 등 녹색성장의 중심으로 녹색기술 개발 전략을 수립 추진해 왔다. 본 연구에서는 대규모 녹색기술 전문가 집단이 참여하여 델파이 조사 기법을 활용한 27대 중점 녹색기술 내 총 131개의 전략제품 서비스 기술을 대상으로 기술수준 조사를 실시하였다. 2011년 기준으로 중점 녹색기술 전체에 대한 주요 5개국의 기술수준은 세계최고기술보유국(미국) 대비 EU(99.4%), 일본(95.3%), 한국(77.7%), 중국(67.1%) 순이며, 한국은 5개국 중 4위를 차지하였다. 세계최고기술보유국(미국)과 한국과의 기술격차년수는 4.1년으로 EU(3.9년), 일본(3.1년)에는 뒤져 있는 반면, 중국에는 2.1년 앞선 것으로 조사되었다. 우리나라의 기술수준이 가장 높은 중점 녹색기술은 '개량형 경수로 설계 및 건설기술(90.1%)'이며, 이어서 '실리콘계 태양전지의 고효율 저가화 기술(85.0%)', '고효율 저공해 차량기술(84.5%)' 순으로 나타났다. 중점 녹색기술의 투자유형에 따른 기술수준은 단기(85.0%), 중기(77.3%), 장기(71.1%) 집중형 순이며, 기술수준이 낮을수록 중장기적인 투자를 요하는 것으로 나타나 전반적인 투자유형 설정은 적절한 것으로 조사되었다.

• Animal Bioscience
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• 제34권6호
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• pp.1078-1087
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• 2021

Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs in vitro. Additionally, to explore the function of CTRP3 in goose SMSCs. Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression. Results: The goose SMSCs were successfully isolated and cultured. The expression of Pax7 and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, MyHC and MyoG. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation. Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose.

• Bulletin of the Korean Chemical Society
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• 제33권12호
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• pp.4003-4006
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• 2012

A novel copper nanoparticles were synthesized from cupric sulfate using hydrazine as reducing reagents. A series of aromatic nitro compounds were reacted with sodium borohydride in the presence of the copper nanoparticles catalysts to afford the aromatic amino compounds in high yields. Additionally, the catalysts system can be recycled and maintain a high catalytic effect in the reduction of aromatic nitro compounds.

• Journal of Microbiology and Biotechnology
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• 제30권7호
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• pp.955-961
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• 2020

Lactic acid bacteria (LAB) have caused many microbiological incidents in the brewing industry, resulting in severe economic loss. Meanwhile, traditional culturing method for detecting LAB are time-consuming for brewers. The present review introduces LAB as spoilage microbes in daily life, with focus on LAB in the brewing industry, targeting at the spoilage mechanism of LAB in brewing industry including the special metabolisms, the exist of the viable but nonculturable (VBNC) state and the hop resistance. At the same time, this review compares the traditional and novel rapid detection methods for these microorganisms which may provide innovative control and detection strategies for preventing alcoholic beverage spoilage, such as improvement of microbiological quality control using advanced culture media or different isothermal amplification methods.

• Structural Engineering and Mechanics
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• 제83권1호
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• pp.19-29
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• 2022

The angle steel frame confined concrete columns (ASFCs) are an emerging form of hybrid columns, which comprise an inner angle steel frame and a concrete column. The inner angle steel frame can provide axial bearing capacity and well confining effect for composite columns. This paper presents the experimental and theoretical studies on the seismic behaviour of ASFCs. The experimental study of the 6 test specimens is presented, based on the previous study of the authors. The theoretical study includes two parts. One part establishes the section analysis model, and it uses to analyze section axial force-moment-curvature. Another part establishes the section moment-curvature hysteresis model. The test and analysis results show that the axial compression ratio and the assembling of steel slabs influence the local buckling of the angle steel. The three factors (axial compression ratio, content of angle steel and confining effect) have important effects on the seismic behaviour of ASFCs. And the theoretical model can provide reasonably accurate predictions and apply in section analysis of ASFCs.

• Asian Pacific Journal of Cancer Prevention
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• 제15권23호
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• pp.10387-10391
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• 2015

Background: This study aimed to identify any association between the p73 gene G4C14-to-A4T14 polymorphism and risk of non-small cell lung cancer (NSCLC) in the south of China. Materials and Methods: We genotyped the p73 gene polymorphism of peripheral blood DNA from 168 patients with NSCLC and 195 normal controls using HRM (high resolution melting) and PCR-CTPP (polymerase chain reaction with confronting two-pair primers). Results: The results of genotyping by HRM and PCR-CTPP were consistent with direct sequencing, the p73 genotype distribution in 168 lung cancer patients being as follows: GC/GC 101 cases (60.1%), GC/AT 59 cases (35.1%), AT/AT 8 cases (4.8%). The carriers of AT/AT genotype had a significantly reduced risk of NSCLC (OR=0.370; 95%CI: 0.170-0.806; p=0.010) as compared with non-carriers. However, we found no relations between p73 genotypes and histological type (p=0.798, $x^2=0.452$), tumor stage (p=0.806, $x^2=0.806$), or lymph node metastasis (p=0.578, $x^2=1.098$). Conclusions: Our findings suggest that the p73 G4C14-to-A4T14 polymorphism may be a modifier of NSCLC susceptibility in the Chinese population.

• Journal of Information Technology Applications and Management
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• 제27권5호
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• pp.35-47
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• 2020

Global waste has become a global issue and we can see the new trend of discovery businesses established to focus on solving the waste problem using new renewable energy technology and the circular economy business model. This paper aims to study factors that impact green entrepreneurship in Thailand, such as environmental concern, organizational environment, founder demographics, education background, entrepreneurship awareness, as well as external factors of a business. The study analyzes the data from three qualitative in-depth interviews with green entrepreneur founders who started the businesses in polymer up-cycling, waste management, and renewable energy. The study finds overseas educational background to be one of the key main drivers for the entrepreneurial courage to decide to pursue a new business venture. By having the exposure toward the different culture, three entrepreneurs hands-on experiential learning through three key drivers who are composed of the can-do attitude, the willingness to be self-employed, and the way of seeking for the freedom to express their passions.

• Journal of Animal Science and Technology
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• 제64권3호
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• pp.432-442
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• 2022

The purpose of this study was to analyse the effects of light colour on rabbit reproductive performance and the expression of key follicular development genes. Rabbits (n = 1,068, 5 months old, 3.6-4.4 kg live body weight) were divided randomly into four groups, housed individually in wire mesh cages and exposed to red, green, blue, and white light-emitting diode (LED) light (control). The lighting schedule was 16 L : 8 D-15 d / 150 lx / 6:00 am-22:00 pm (3 d preartificial insemination to 12 d postartificial insemination). Red light and white light affected the conception rate and kindling rate and increased the total litter size at birth (p < 0.05). The effects of red light on litter size at weaning, litter weight at weaning, and individual weight at weaning increased compared with the green and blue groups. The effects of red light on live litter size at birth were increased compared with those in the blue group (p < 0.05). Compared to white light, green and blue light reduced the number of secondary follicles (p < 0.05). Compared to red light, green and blue light reduced the number of tertiary follicles (p < 0.05). Compared with white light, red LED light resulted in greater ovarian follicle stimulating hormone receptor and luteinizing hormone receptor mRNA expression (p < 0.05). Compared with green and blue LED light, red LED light resulted in greater B-cell lymphom-2 mRNA expression (p < 0.05). Compared with green LED light, red LED light inhibited FOXO1 mRNA expression in rabbit ovaries (p < 0.05). Red light can affect the reproductive performance of female rabbits and the expression of key genes for follicular development.