• Title/Summary/Keyword: Karlsruhe

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Genomic Barcode-Based Analysis of Exoelectrogens in Wastewater Biofilms Grown on Anode Surfaces

  • Dolch, Kerstin;Wuske, Jessica;Gescher, Johannes
    • Journal of Microbiology and Biotechnology
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    • v.26 no.3
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    • pp.511-520
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    • 2016
  • The most energy-demanding step of wastewater treatment is the aeration-dependent elimination of organic carbon. Microbial fuel cells (MFCs) offer an alternative strategy in which carbon elimination is conducted by anaerobic microorganisms that transport respiratory electrons originating from carbon oxidation to an anode. Hence, chemical energy is directly transformed into electrical energy. In this study, the use and stability of barcode-containing exoelectrogenic model biofilms under non-axenic wastewater treatment conditions are described. Genomic barcodes were integrated in Shewanella oneidensis, Geobacter sulfurreducens, and G. metallireducens. These barcodes are unique for each strain and allow distinction between those cells and naturally occurring wild types as well as quantification of the amount of cells in a biofilm via multiplex qPCR. MFCs were pre-incubated with these three strains, and after 6 days the anodes were transferred into MFCs containing synthetic wastewater with 1% wastewater sludge. Over time, the system stabilized and the coulomb efficiency was constant. Overall, the initial synthetic biofilm community represented half of the anodic population at the end of the experimental timeline. The part of the community that contained a barcode was dominated by G. sulfurreducens cells (61.5%), while S. oneidensis and G. metallireducens cells comprised 10.5% and 17.9%, respectively. To the best of our knowledge, this is the first study to describe the stability of a synthetic exoelectrogenic consortium under non-axenic conditions. The observed stability offers new possibilities for the application of synthetic biofilms and synthetically engineered organisms fed with non-sterile waste streams.

3-Dimensional Analysis of the Steam-Hydrogen Behavior from a Small Break Loss of Coolant Accident in the APR1400 Containment

  • Kim Jongtae;Hong Seong-Wan;Kim Sang-Baik;Kim Hee-Dong;Lee Unjang;Royl P.;Travis J. R.
    • Nuclear Engineering and Technology
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    • v.36 no.1
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    • pp.24-35
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    • 2004
  • In order to analyze the hydrogen distribution during a severe accident in the APR1400 containment, GASFLOW II was used. For the APR1400 NPP, a hydrogen mitigation system is considered from the design stage, but a fully time-dependent, three-dimensional analysis has not been performed yet. In this study GASFLOW code II is used for the three-dimensional analysis. The first step to analysis involving hydrogen behavior in a full containment with the GASLOW code is to generate a realistic geometry model, which includes nodalization and modeling of the internal structures such as walls, ceilings and equipment. Geometry modeling of the APR1400 is conducted using GUI program by overlapping the containment cut drawings in a graphical file format on the mesh view. The total number of mesh cells generated is 49,476. And the calculated free volume of the APR1400 containment by GASFLOW is almost the same as the value from the GOTHIC modeling. A hypothetical SB-LOCA scenario beyond design base accident was selected to analyze the hydrogen behavior with the hydrogen mitigation system. The source of hydrogen and steam for the GASFLOW II analysis is obtained from a MAAP calculation. Combustion pressure and temperature load possibilities within the compartments used in the GOTHIC analysis are studied based on the Sigma-Lambda criteria. Finally the effectiveness of HMS installed in the APR1400 containment is evaluated from the point of severe accident management

Thermal Inactivation of Horseradish Peroxidase for the Range of Temperatures $110^{\circ}{\sim}140^{\circ}C$ ($110^{\circ}C$ 이상에서의 Peroxidase의 열에 의한 불활성화)

  • Park, K.H.;Stahl, R.;Srimani, B.N.;Loncin, M.
    • Korean Journal of Food Science and Technology
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    • v.9 no.2
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    • pp.165-169
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    • 1977
  • An apparatus for continuous sterilization of fluids in which heating-up and cooling time are negligible enabled determination of the kinetics of thermal inactivation of peroxidase for the range of temperatures $110{\sim}140^{\circ}C$. The enthalpy of activation was 146.4 kJ/mol; free energy of activation, 113kJ/mol; and the entropy of activation, 82.9J/mol.K. Comparisons of the experimental results with the thermal destruction time curves of microorganisms showed the possibility that the time required to inactivate peroxidase might be taken into account in evaluating thermal processes for commerciel HTST methods.

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MBR technology for textile wastewater treatment: First experience in Bangladesh

  • Saha, Pradip;Hossain, Md. Zakir;Mozumder, Md. Salatul I.;Uddin, Md. Tamez;Islam, Md. Akhtarul;Hoinkis, Jan;Deowan, Shamim A.;Drioli, Enrico;Figoli, Alberto
    • Membrane and Water Treatment
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    • v.5 no.3
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    • pp.197-205
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    • 2014
  • For the first time in Bangladesh, a bench scale membrane bioreactor (MBR) unit was tested in treating a textile wastewater in the industry premises of EOS Textile Mills LTD, Dhaka for three months. The performance of the unit was compared with that of the conventional activated sludge treatment plant, which is in operation in the same premises. The COD and BOD removal efficiency of the MBR unit reached to around 90% and 80% respectively in 20 days whereas the removal efficiency of the conventional treatment plant was as low as 40-50% and 38-40% respectively. The outlet COD and the BOD level for the MBR unit remained stable in spite of the fluctuation in the feed value, while the conventional effluent treatment plant (ETP) failed to keep any stabilized level. The performance of the MBR unit was much superior to that of the functional ETP and the water treated by the MBR system can meet disposal standard.

Transmission Electron Microscopy on Memristive Devices: An Overview

  • Strobel, Julian;Neelisetty, Krishna Kanth;Chakravadhanula, Venkata Sai Kiran;Kienle, Lorenz
    • Applied Microscopy
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    • v.46 no.4
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    • pp.206-216
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    • 2016
  • This communication is to elucidate the state-of-the-art of techniques necessary to gather information on a new class of nanoelectronic devices known as memristors and related resistive switching devices, respectively. Unlike classical microelectronic devices such as transistors, the chemical and structural variations occurring upon switching of memristive devices require cutting-edge electron microscopy techniques. Depending on the switching mechanism, some memristors call for the acquisition of atomically resolved structural data, while others rely on atomistic chemical phenomena requiring the application of advanced X-ray and electron spectroscopy to correlate the real structure with properties. Additionally, understanding resistive switching phenomena also necessitates the application not only of pre- and post-operation analysis, but also during the process of switching. This highly challenging in situ characterization also requires the aforementioned techniques while simultaneously applying an electrical bias. Through this review we aim to give an overview of the possibilities and challenges as well as an outlook onto future developments in the field of nanoscopic characterization of memristive devices.

DEVELOPMENT OF THE MATRA-LMR-FB FOR FLOW BLOCKAGE ANALYSIS IN A LMR

  • Ha, Kwi-Seok;Jeong, Hae-Yong;Chang, Won-Pyo;Kwon, Young-Min;Cho, Chung-Ho;Lee, Yong-Bum
    • Nuclear Engineering and Technology
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    • v.41 no.6
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    • pp.797-806
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    • 2009
  • The Multichannel Analyzer for Transient and steady-state in Rod Array - Liquid Metal Reactor for Flow Blockage analysis (MATRA-LMR-FB) code for the analysis of a subchannel blockage has been developed and evaluated through several experiments. The current version of the code is improved here by the implementation of a distributed resistance model which accurately considers the effect of flow resistance on wire spacers, by the addition of a turbulent mixing model, and by the application of a hybrid scheme for low flow regions. Validation calculations for the MATRA-LMR-FB code were performed for Oak Ridge National Laboratory (ORNL) 19-pin tests with wire spacers and Karlsruhe 169-pin tests with grid spacers. The analysis of the ORNL 19-pin tests conducted using the code reveals that the code has sufficient predictive accuracy, within a range of 5 $^{\circ}C$, for the experimental data with a blockage. As for the results of the analyses, the standard deviation for the Karlsruhe 169-pin tests, 0.316, was larger than the standard deviation for the ORNL 19-pin tests, 0.047.

A Performance Analysis of Large ABox Reasoning in OWL-DL Reasoners (다양한 OWL-DL 추론 엔진에서 대용량 ABox 추론에 대한 성능평가)

  • Seo, Eun-Seok;Park, Young-Tack
    • Journal of KIISE:Software and Applications
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    • v.34 no.7
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    • pp.655-666
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    • 2007
  • Reasoners using typical Tableaux algorithm such as RacerPro, Pellet have a problem in Tableaux algorithm large ABox reasoning. Researches to solve these Problems are dealt with Instance Store of University of Manchester which uses Tableaux algorithm based reasoner and DBMS and KAON2 of University of Karlsruhe using Disjunctive Datalog approach. An evaluation experiment for present reasoners is the experiment of TBox reasoning in most of Tableaux algorithm based one. The most of benchmarking tests in reasoning systems haven't done with ABox reasoning based Tableaux Algorithm but done with TBox reasoning based Tableaux Algorithm. Especially, rarely reported benchmarking tests in reasoners have been issued nowadays. Therefore, this thesis evaluates systems with theory of each reasoners for large ABox reasoning that becomes issues recently with typical reasoners. The large AoBx reasoning engine will be analyzed using Instance Store and KAON2 of Manchester University for large ABox processing. At the analysing method, LUBM(Lehigh University BenchMark), benchmarking test method, and it's test system will be introduced. In conclusion, I recommend appropriate reasoner in various environment with experiment result and characteristic of algorithm used for each reasoner.