• Preventive Nutrition and Food Science
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• v.22 no.3
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• pp.231-236
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• 2017

Fermented kale juices using four types of lactobacilli were produced in the present study. After 48 h of fermentation time, viable cell counts of all ferments reached an above $10^9CFU/mL$. The viability of the ferments after cold storage in the refrigerator for 4 weeks showed $10^8CFU/mL$ in all ferments. Among four types of fermented kale juices, the ferment of Lactobacillus acidophilus IFO 3025 indicated a good nutritional composition, including neutral sugar ($1,909.76{\mu}g/mL$), reducing sugar ($564.00{\mu}g/mL$, P<0.05), and protein contents ($160.06{\mu}g/mL$, P<0.05). The results of mineral composition analysis had the highest potassium value in all ferments ($854.16{\sim}895.07{\mu}g/mL$), particularly in the ferment of Lactobacillus brevis FSB-1 (P<0.001), which is necessary to sustain osmotic pressure, prevention of high blood pressure, and protein synthesis. Moreover, calcium, phosphorus, and magnesium contents related to bone health were generally sufficient in all ferments. Consequently, in this study, fermented kale juices may be suggested as a healthy fermented beverage with essential nutrients. However, the acceptability of the fermented kale juice to the Korean taste should be further investigated with a trained taste panel to determine whether inoculated fermentation could be an option for the consumers.

• Food Science and Preservation
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• v.6 no.4
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• pp.417-423
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• 1999

Quality changes of crown daisy and kale were investigated during storage at 10$^{\circ}C$ after washing with cooled electrolyzed acid water at 3 times in 2 min. Total count and coliform count of crown daisy and kale after immersion in electrolyzed acid water were decreased to 1/130 and 1/1,170 of non-immersed crown daisy and to l/870 and l/470 of non-immersed kale. However total count and coliform count were increased to the similar levels of non-immesed and tap water immersed one after 6 days of storage. Weight loss of crown daisy and ka1e were lower than others for 3 days of storage but higher than that of one after that time. Decay rate of crown daisy and kale immersed electrolyzed acid water showed lower than that of non-immersed and tap water immersed one for 6 days. In case of kale, rupture strength was higher than others at just after immersion and showed similar values after initial storage period. Color value of both crown daisy and kale showed high L, b and low a value in the order of electrolyzed acid water, tap water and non-treatment. Chlorophyll content of crown daisy and kale were lower than those of others just after immersion in electrolyzed acid water, but showed rapid reduction in the order of non-treatment. tap water and electrolyzed acid water after 6 days. Overall organoleptic properties of crown daisy and kale in immersion electrolyzed acid water were higher than those of others.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.5
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• pp.668-674
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• 2014

The objective of the present study was to investigate the protective and free radical scavenging effects of conventionally and organically cultivated kale juices against oxidative damage in $LLC-PK_1$ cells. The DPPH, NO, $O_2{^-}$, and ${\cdot}OH$ radical scavenging activities of organically cultivated kale were higher than those of conventionally cultivated kale juice. Oxidative damage induced by AAPH (2,2'-azobis(2-amidinopropane) dihydrochloride), SNP (sodium nitroprusside), pyrogallol, and SIN-1 (3-morpholinosydnonimine) led to loss of cell viability and increased lipid peroxidation in LLC-PK1 cells, whereas treatment with vegetable juices, especially organically cultivated kale juices, significantly increased cell viability and inhibited lipid peroxidation in a dose-dependent manner (P<0.05). These results suggest that organically cultivated kale juices have protective roles against oxidative stress induced by free radicals.

• Korean Journal of Environmental Agriculture
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• v.41 no.2
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• pp.125-134
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• 2022

BACKGROUND: It is a very important task to block distribution of the agricultural products contaminated with pesticides in advance to protect consumers from residual pesticides among the agricultural products. Therefore, this study was performed to determine residual characteristics of pesticides in time-dependent manner and present scientific evidences for pre-harvest residue limit (PHRL) setting. METHODS AND RESULTS: The tested pesticides, chromafenozide and pyridalyl were sprayed onto the kale twice (seven day intervals) and then the plant samples were collected at 0 (after 3 hours), 1, 2, 3, 5, 7, and 10 days after the last spraying. Residual concentration of chromafenozide in kale decreased with 74.9% (of decreased fraction, field 1) and 85.3% (field 2) and pyridalyl decreased with 81.2% (field 1) and 85.8% (field 2), calculated by comparisons of the concentrations at 0 day and 10 days. Also biological half-lives of chromafenozide in kale were 5.6 day (field 1) and 3.4 day (field 2), and those of pyridalyl were 4.3 day (field 1) and 3.5 day (field 2). CONCLUSION(S): If the residues of chromafenozide and pyridalyl in kale from 10 days before harvest are less than 37.6 mg/kg and 58.9 mg/kg, respectively, it is expected that safe kale below MRL can be supplied on the pre-harvest day.

• Korean Journal of Environmental Agriculture
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• v.36 no.2
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• pp.97-105
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• 2017

BACKGROUND : Environmental stress has a major effect on the growth and yields of vegetables, and can significantly affect nutritionally important phytochemicals, causing large economic losses. METHODS AND RESULTS : The present study was aimed at exploring the effects of water stress on the carotenoid and proline contents in kale leaves to understand drought tolerance of kale plants. Kale was randomly divided into two groups at 57 days after sowing (DAS). One of the groups was well-watered (WW) and the other was water stressed (WS). Harvesting of kale leaves was started one day after treatment (58 DAS) and continued for 10 days (~67 DAS). We investigated the status of plant growth (leaf number, length, width, fresh weight) of kale throughout the study. Carotenoid (lutein, ${\alpha}-carotene$, zeaxanthin, ${\beta}-carotene$) and proline contents were analyzed by high-performance liquid chromatography (HPLC). Our results showed that the total carotenoid contents ranged from 926.0 to 1,212.0 mg/kg dry wt. (at 3 and 2 days, respectively) in WW treatment and 887.8 to 1,157.4 mg/kg dry wt. (at 10 and 4 days, respectively) in WS treatment. The ratio of individual carotenoid to the total carotenoid contents of kale leaves was 51.4 for lutein, 4.44 for zeaxanthin, 2.76 for ${\alpha}-carotene$, and 41.4% for ${\beta}-carotene$. Total carotenoid contents showed a significant reduction from 7 days (1,037.2 mg/kg dry wt.) to 10 days (887.8 mg/kg dry wt.) in WS treatment. The lutein content did not show a significant difference in WW between 7 and 10 days after treatment but showed a significant difference in WS treatment. The ${\alpha}-carotene$ content showed no significant difference between the treatments. However, zeaxanthin content was higher during 4-10 days and ${\beta}-carotene$ content was lower during 6-10 days in WS than in WW on each harvest day. In WW, the proline content showed no significant difference, but in WS, the proline content started to increase at 7 days and almost doubled in 10 days. CONCLUSION : The marked increase in zeaxanthin and proline contents in kale leaves indicated that the two phytochemicals are associated with drought tolerance in the plant.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.332-342
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• 2022

Kale (Brassica oleracea var. acephala) is one of the most frequently consumed leafy vegetables globally, as it contains numerous nutrients; essential amino acids, phenolics, vitamins, and minerals, and is particularly rich in glucosinolates. However, the differences in the biosynthesis of glucosinolates and related gene expression among kale cultivars has been poorly reported. In this study, we investigated glucosinolates profile and content in three different kale cultivars, including green ('Man-Choo' and 'Mat-Jjang') and red kale ('Red-Curled') cultivars grown in a vertical farm, using transcriptomic and metabolomic analyses. The growth and development of the green kale cultivars were higher than those of the red kale cultivar at 6 weeks after cultivation. High-performance liquid chromatography (HPLC) analysis revealed five glucosinolates in the 'Man-Choo' cultivar, and four glucosinolates in the 'Mat-Jjang' and 'Red-Curled' cultivars. Glucobrassicin was the most predominant glucosinolate followed by gluconastrutiin in all the cultivars. In contrast, other glucosinolates were highly dependent to the genotypes. The highest total glucosinolates was found in the 'Red-Curled' cultivar, which followed by 'Man-Choo' and 'Mat-Jjang'. Based on transcriptome analysis, eight genes were involved in glucosinolate biosynthesis. The overall results suggest that the glucosinolate content and accumulation patterns differ according to the kale cultivar and differential expression of glucosinolate biosynthetic genes.

• Journal of Biosystems Engineering
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• v.43 no.1
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• pp.30-36
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• 2018

Purpose: The objective of this study is to evaluate the effect of cultivation conditions on the growth and glucosinolate content of Chinese cabbage and kale. Methods: Chinese cabbage and kale were grown in three different cultivation conditions, including a plant factory, greenhouse, and open field. Samples were collected at two harvesting times (10 d and 20 d after transplanting the seedlings). Nine growth parameters (plant height, plant width, number of leaves, petiole diameter, SPAD readout, leaf length, leaf width, stem diameter, and plant weight) were measured immediately after harvesting, and the samples were freeze-dried and stored until the glucosinolate content was analyzed. Mean values of the growth parameters and glucosinolate contents were evaluated using Duncan's multiple range tests. Results: The results indicated that the plant parameters of the Chinese cabbage and kale were greater for plants grown in the plant factory and greenhouse. The plant height, width, and weight showed significant differences in the Duncan's multiple range tests at a 5% level. The plant factory also produced greater contents of most of the glucosinolates. Conclusions: Three different cultivation conditions significantly affected the growth and glucosinolate contents of Chinese cabbage and kale. Further study is necessary to investigate other functional components and different vegetable varieties.

• Journal of Life Science
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• v.5 no.1
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• pp.26-32
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• 1995

The antimutagenic effects of insoluble dietary fibers(IDF) extracted from some green-yellow vegetables(kale, carrot, spinach, broccoli and soybean sprout) and soybean by binding the carcinogens of MeIQ (2-amino-3,4- dimethyl-imidazo(4,5-f) quinoline) and Trp-P-2(3-amino-1-methyl-5H-pyrido[4,3-b] indole) in Salmonella tylhimirium TA100 and TA98 were studied. All of the insoluble dietary fiber samples which binded MeIQ exhibited high antimutagenic effects by removing the mutagen. Among the samples, IDFs from kale and soybean showed strong binding capacity fo the carcinogen and revealed about 90% of the antimutagenic activity. the IDF samples showed somewhat lower binding capacity to the Trp-p-2. The lignin which extracted from kale, soybean and carrot, and the cellulose strongly removed the mutagenicity of MeIQ by the binding. Among the samples, the level of lignin in kale revealed the highest(about 10%), and it seemed that the higher content of lignin in kale is one of the reasons to increase its antimutagenic effect.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.1
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• pp.59-64
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• 1991

Studies comparing the relative effects of water soluble extract of Ganoderma lucidum kale juice and sodium dextrothyroxine on lipid component in liver brain and testis of hypercholeste-rolemic rats for 4 weeks. Therefore the aim of this work was to investigate for prevention in cardiovascular disease, Total cholesterol concentration in liver were significantly lower kale juice and sodium dextrothyroxine(1.25mg/kg diet) fed groups than the other groups and trigly-ceride concentration was significantly lower Ganodrma lucidum fed group than in control group;. Total cholesterol concentration in testis were lower other groups than in control group and triglyceride concentration were significantly lower cholesterol Ganoderma lucidum and kale juice groups in control group. Phospholipid concentration in testis was higher sodium dextroth-yroxine fed group thanin control group.

• Preventive Nutrition and Food Science
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• v.17 no.4
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• pp.269-273
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• 2012

Shinseoncho and kale were divided into stem [shinseoncho stems (SS) and kale stems (KS)] and leaf parts [shinseoncho leaves (SL) and kale leaves (KL)] and made into green vegetable juices for analyses of nutritional compositions and antioxidant activities. Higher values of total acidity were observed in SL (0.736%) and KL (0.841%) than in SS (0.417%) and KS (0.335%) (p<0.05). Neutral sugar content showed higher values in SS (21.740 mg/mL) and SL (18.657 mg/mL) when compared with KS (1.497 mg/mL) and KL (1.452 mg/mL) (p<0.05). Protein content showed the highest value in SL (7.610 mg/mL) (p<0.05), while SS (0.403 mg/mL) and KS (0.403 mg/mL) showed similar lower values. Total polyphenol contents of SL (423.139 ${\mu}g/mL$) was significantly higher value (p<0.05) than those of other samples, which occurred in the following order: SL>KL (218.494 ${\mu}g/mL$) KS (107.269 ${\mu}g/mL$)>SS (75.894 ${\mu}g/mL$). KL exerted the highest DPPH radical scavenging activity (84.834%) (p<0.05), which occurred in the following order: KL>SL (63.473%)>KS (52.894%)>SS (35.443%). ABTS radical scavenging activity showed that SL (66.088%) and KL (38.511%) had higher scavenging activities, whereas SS (7.695%) and KS (9.609%) demonstrated to be lower activities (p<0.05). In general, leaf parts had much higher antioxidant activities as well as total polyphenol contents than those of the stem parts. In conclusion, shinseoncho and kale, particularly their leaf parts, offer antioxidant properties in green vegetable juices and the consumption of them may be beneficial as a nutrition source and in health protection.