Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Glucosinolate Content Varies and Transcriptome Analysis in Different Kale Cultivars (Brassica oleracea var. acephala) Grown in a Vertical Farm

수직농장에서 자란 케일(Brassica oleracea var. acephala) 품종에 따른 글루코시놀레이트 함량의 변화 및 전사체 분석

  • Nguyen, Thi Kim Loan (Division of Horticultural Science, Gyeongsang National University) ;
  • Lee, Ga Oun (Department of GreenBio Science, Gyeongsang National University) ;
  • Jo, Jung Su (Department of Horticultural Science, College of Agricultural & Life Science, Institute of Agricultural Science and Technology, Kyungpook National University) ;
  • Lee, Jun Gu (Department of Horticulture, College of Agriculture & Life Sciences, Jeonbuk National University) ;
  • Lee, Shin-Woo (Department of Plant & Biomaterials Science, Gyeongsang National University) ;
  • Son, Ki-Ho (Division of Horticultural Science, Department of GreenBio Science, Gyeongsang National University)
  • 응웬티김로안 (경상국립대학교 원예과학부) ;
  • 이가운 (경상국립대학교 생명자원과학과) ;
  • 조정수 (경북대학교 농업생명과학대학 원예과학과 농업과학기술연구원) ;
  • 이준구 (전북대학교 농업생명과학대학 원예학과) ;
  • 이신우 (경상국립대학교 항노화신소재과학과) ;
  • 손기호 (경상국립대학교 원예과학부 생명자원과학과)
  • Received : 2022.07.22
  • Accepted : 2022.10.14
  • Published : 2022.10.31
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Abstract

Kale (Brassica oleracea var. acephala) is one of the most frequently consumed leafy vegetables globally, as it contains numerous nutrients; essential amino acids, phenolics, vitamins, and minerals, and is particularly rich in glucosinolates. However, the differences in the biosynthesis of glucosinolates and related gene expression among kale cultivars has been poorly reported. In this study, we investigated glucosinolates profile and content in three different kale cultivars, including green ('Man-Choo' and 'Mat-Jjang') and red kale ('Red-Curled') cultivars grown in a vertical farm, using transcriptomic and metabolomic analyses. The growth and development of the green kale cultivars were higher than those of the red kale cultivar at 6 weeks after cultivation. High-performance liquid chromatography (HPLC) analysis revealed five glucosinolates in the 'Man-Choo' cultivar, and four glucosinolates in the 'Mat-Jjang' and 'Red-Curled' cultivars. Glucobrassicin was the most predominant glucosinolate followed by gluconastrutiin in all the cultivars. In contrast, other glucosinolates were highly dependent to the genotypes. The highest total glucosinolates was found in the 'Red-Curled' cultivar, which followed by 'Man-Choo' and 'Mat-Jjang'. Based on transcriptome analysis, eight genes were involved in glucosinolate biosynthesis. The overall results suggest that the glucosinolate content and accumulation patterns differ according to the kale cultivar and differential expression of glucosinolate biosynthetic genes.

케일(Brassica oleracea var. acephala)은 필수 아미노산, 비타민, 미네랄과 같은 수많은 영양소를 함유하고 특히 글루코시놀레이트가 풍부하기 때문에 전 세계적으로 가장 많이 소비되는 잎 채소 중 하나이다. 그러나 케일 품종 간의 글루코시놀레이트 합성과 관련된 유전자 발현에 대한 연구는 미비한 실정이다. 본 연구에서는 전사체 및 대사체 분석을 사용하여 식물공장에서 재배된 녹색(만추 및 맛짱) 및 적색 케일 품종(적곱슬)을 포함한 3 가지 케일 품종에서 글루코시놀레이트를 조사하였다. 재배 후 6주된 녹색 케일 품종의 생육 및 발달이 적색 케일 품종에 비해 높았다. High-performance liquid chromatography (HPLC) 분석에서 7가지 글루코시놀레이트를 분석하였다; 만추 품종에서는 5종의 글루코시놀레이트가, 맛짱과 적곱슬 품종에서는 4종의 글루코시놀레이트가 분류되었다. Glucobrassicin은 3가지 케일 품종에서 가장 높은 글루코시놀레이트 였다. 총 글루코시놀레이트 함량은 적곱슬 품종에서 가장 높았다. 전사체 분석에서는 8개의 유전자가 글루코시놀레이트 합성에 관여됨을 확인할 수 있었다. 이러한 결과는 케일 품종에 따라 글루코시놀레이트 함량과 축적 패턴이 다르다는 것을 시사한다.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2018R1C1B5086600).

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