• Korean Journal of Agricultural and Forest Meteorology
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• v.1 no.2
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• pp.90-96
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• 1999

Foliar and physiological characteristics of Schisandraceae plants distributed in Korea were investigated to elucidate differences in growth and the variations of inter and intra species of the plants. For this study, three species of Schisandraceae plants were used; i) Schisandra chinensis, decidious broad-leaved tree, ii) S. nigra, decidious broad-leaved tree, including monoecious, male and female types, ii) Kadsura japonica, evergreen broad-leaved tree. There were no significant differences in leaf length and width among three species, but leaves of Schisandra sp. plants were shorter and wider relative to those of K. japonica. The length of petiole of Schisandra sp. plants was greater than that of K. japonica and the difference was evident between female type of S. chinensis and K. japonica as longer as two times. Schisandra sp. trees showed larger size of stomata, but fewer numbers of stomata, compared with K. japonica. Although substantial changes in leaf morphology were not found in the Schisandraceae plants grown in different environmental conditions, the foliar length of K. japonica was seemed to be increased with increasing an altitude. Chlorophyll contents were also variable in inter and intra species and in, general K. japonica showed higher levels of chlorophyll contents relative to Schisandra sp. plants. K. japonica also showed higher light compensation point(LCP), light saturation point(LSP) and $CO_2$ assimilation rates at LSP, compared with Schisandra sp. trees. However, Schisandra sp. trees showed lower respiration per photosynthesis rates at LSP. The finding suggested that Schisandra sp. trees were acclimated to the growth conditions of lower light intensity with more effective photosynthetic activity. This was also confirmed by the fact that Schisandra sp. plants grown in shading places of a forest demonstrated the elevated rates of net $CO_2$ assimilation under the conditions of low light intensity.

• Korean Journal of Medicinal Crop Science
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• v.15 no.3
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• pp.162-169
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• 2007

Immune activities of two different extracts of Kadsura japonica Dunal. by typical extraction processes using water and ethanol at 60 $^{\circ}$C and 100 $^{\circ}$C were compared to them by ultrasofication system and through traditional fermemtation process. The fermented broth Kadsura japonica Dunal. definitely improved the growth of human B and T cell up to 30% and 22%, respectively, compared to the control. The secretion of TNF-${\alpha}$ and IL-60 was also enhanced by the addition of the fermented broth, up to 35%. NK cell activation was significantly improved up to 1.4 times higher than the case of adding other extracts. It was also found that this broth could yield higher nitric oxide production from macrophage than Lipopolysaccharides (LPS). It can be concluded that Kadsura japonica has immune activities and, in general, the culture broth from a conventional fermentation has higher immune activities, possibly by yielding immuno-modulatory compounds, not existed in typcial extraction systems as the result of HPLC analysis.

• The Korean Journal of Ecology
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• v.4 no.1_2
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• pp.8-24
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• 1981

This paper contains the results obtained by ecological investigation of flora in Sogumoon Island of the southern coast in Korea. Fifty varieties, 455 species, 322 genera and 104 family of Tracheophyta were recorded island showed a plant community predcominated by Pinus thunbergii grwon in miggle with Castanopsis cuspidata var. sieboldii, Machilus thunbergii, Litsea japonica, Albizzia julibrissin, Carpinus coreana, Carpinus laxiflora, Mallotus japonica and Clerodendron trichotomum. Sixtythree species in Sogumoon Island and 64 species in Pogil Island of evergreen broad-leaved trees were recorded by this investitgations. The plant resources of this island were 203 species. The evergreen broad-leaved trees of this island wre valuable for landscaping material plants and the evergreenherbs were valuable for foliage-plants resources. The flora breaking in this island was due to cuttinf for fuel and grazing cattle and goats. Therefore Machilus thunbergii, Ardisia japonica, Ficus miponica, Kadsura japonica, Daphniphyllum macropodum, etc. should be preserved. Autochonous flora of Buxus microphyllac, Fius erecta, Machilus thunbergii, Kadsura japonica, Ardisia japonica, Mitchella undulata, etc. should be preserved.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.87-87
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• 2022

Under the COVID-19 pandemic, interest in immune enhancement and anti-obesity is increasing. Thus, in this study, we investigated whether Kadsura japonica fruits (KJF) exhibits immunostimulatory activity and anti-obesity activity. KJF increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. Inhibition of TLR2 and TLR4 blocked KJF-mediated production of immunostimulatory factors in RAW264.7 cells. In addition, the inhibition of MAPK and PI3K/AKT signaling pathway reduced KJF-mediated production of immunostimulatory factors, and the activation of MAPK and PI3K/AKT signaling pathway by KJF suppressed the inhibition of TLR2/4. KJF attenuated the lipid accumulation and the protein expression such as CEBPα, PPARγ, perilipin-1, adiponectin, and FABP4 related to the lipid accumulation in 3T3-L1 cells. In addition, KJF inhibited excessive proliferation of 3T3-L1 cells and protein expressions such as β-catenin and cyclin D1 related to cell growth. These findings indicate that KJF may have immunostimulatory activity and anti-obesity activity.

• Korean Journal of Plant Resources
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• v.23 no.1
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• pp.31-37
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• 2010

It was investigated that morphological characteristics of leaf and stomata, chlorophyll contents and wood anatomy characteristics in Schisandra chinensis, Schisandra nigra and Kadsura japonica. Leaf morphological characteristics were significant at 5% levels within the species and within the individuals as a male, a female and monoecious. K. japonica was the longest in the leaf length, followed by S. chinensis and S. nigra. For the leaf length among S. nigra individuals, the female was longer than male and monoecious. As for the Petiole length, S. nigra was twice longer than S. chinensis and K. japonica. Stomata length was ranged from $92.5\;{\mu}m$ to $105.9\;{\mu}m$ for S. nigra, $79.9\;{\mu}m$ for S. chinensis, $76.2\;{\mu}m$ for K. japonica. S. nigra was longer than the others in length and width, while its stomata density was lower than the others. The size of vessel elements and xylem fiber showed a similar tendency to stomata, significant at 5% levels within S. nigra individuals (male, female and monoecious). Chlorophyll contents were also variable in inter and intra species and in general K. japonica showed higher levels of chlorophyll contents than Schisandra spp. plants.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.1
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• pp.72-82
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• 1999

The objective of this study was to covering of vine the traffic noise barrier and analyse of their growth characteristics. For the purpose of the experiments, chosen were 28 plants. In January 1996 to December 1998, the nursery seedbeds on the Chonju Arboretum in Korea Highway Corporation were seeding and cutting with those species, and a few species were carried out Honam Highway field experiments. The results are summarized as follows ; The germination ratio of seedbed experiments were Wisteria floribunda(88%), Lonicera japonica(86%), Parthenocissus tricuspidata, Paederia scandens(85%), Celastrus orbiculatus(76%), Clematis terniflora var. denticulata(52%), Clematis mandshurica(44%) respectively. The rooting ratio of cutting experiments were Trachelospermum asiaticum var. intermedium(88%), Parthenocissus quinquefolia(87%), Trachelospermum asiaticum var. asiatica(85%), Kadsura japonica(82%), Hedera rhombea(81%), Euonymus fortunei var. radicans(83%), Trachelospermum jasminoides var. pubescens(80%), Lonicera japonica cv. Aureo-reticulata(80%), Trachelospermum asiaticum var. majus(78%), Euonymus radicans cv. Aureo-marginata(66%), in descending order. As a conclusion, this study shows that the traffic noise barrier was effected by Paederia scandens, Lonicera japonica, Parthenocissus quinquefolia, Wisteria floribunda, Parthenocissus tricuspidata in the field experiments and the others were necessary to maintainable management.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.309-313
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• 1998

RAPD (Random Amplified Polymorphic DNA) analysis was conducted to Schisandra nigra plants in order to select the specific markers for monoecious and dioecious individuals. RAPD results using eighty random 10-mer primers revealed that S. nigra had a different banding pattern from S. chinensis and Kadsura japonica. When DNA isolated from leaves of monoecious and dioecious plants were used as PCR template, only five primers, OPA-17, OPA-19, OPB-03, OPB-09 and OFB-16, showed polymorphic band patterns. No variation in banding profiles within male or female individuals was observed when these five primers were used whereas three monoecious plants (No 1, No 2 and No 3) showed different banding patterns one another, A 750 bp segment was amplified by primer OPB-3 from male individuals. On the other hand, two segments, 950 bp and 1690 bp, with OPA-19 and 700 bp of segment with OPB-3 were amplified in female individuals. These result indicate that the specific buds of male and female S. nigra could be used as genetic markers for the early discrimination of male and female individuals.

• Korean Journal of Environment and Ecology
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• v.8 no.1
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• pp.27-42
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• 1994

Vegetational structure and successional sere were investigated for evergreen broad-leaved forest in Hongdo. Forty-nine plots were classified into six groups, which are Camellea japonica community, Machilus thunbergii community, Castanopsis cuspidata var. sieboldii-Machilus thunbergii community, Castanopsis cuspidata var. sieboldii community, Castanopsis cuspidata var. sieboidii-Camellea japonica community and evergreen broad-leaved mixed community by the DCA ordiantion and TWINSPAN techniques. Successional sere of the forest vegetation in the surveyed area were proceeding from Pinus densiflora through Castanopsis cuspidata var. sieholdii to Neolitsea sericea, Dendropanax morbifera, Machilus japonica, Quercus acuta and Mahilus thunbergii in the canopy layer group and from Ligustrum japonicum, Ardisia japonica. Eurya japonica. Rhaphiolepsis umbellata through Trachelosperum asiaticum, Kadsura japonica. Litsia Japonica, Camellea japonica to Aucuba japonica, Euscaphis japonica, Ficus erecta in the understory and shrub layer group.

• Journal of the Korean Wood Science and Technology
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• v.38 no.6
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• pp.579-586
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• 2010

This study was carried out to evaluate the antifungal and antioxidative activities of ethanol extracts from Magnoliaceae to investigate the possibility for the natural fungicides and food preservatives. The antifungal activities of ethanol extracts were evaluated as a hyphal growth inhibition rate using four plant pathogenic and five wood rot fungi. The high inhibition activity on the growth of fungi was shown in bark of Magnolia obovata that indicated more than 50% hyphal growth inhibition rate except Trametes versicolor, one of the white rot fungi. The antifungal activity was the highest in the ethanol extracts from M. obovata and the following was in order of M. kobus and M. sieboldii. The extract from bark in M. obovata showed higher antifungal activity than that from wood in the same species. Especially, the extracts from flower of M. denudata and M. liliflora indicated the high antifungal activities, while the other portions of same plants showed the low activities. On the other hand, a free radical scavenging method was adopted with 1,1-diphenyl-2-picrylhydrohydrazyl (DPPH) in order to test the antioxidative activities of ethanol extracts. The free radical scavenging activity was very high in the extracts from branch of Schizandra nigra and Kadsura japonica that showed more than 90% at the concentration of 100 ${\mu}g/m{\ell}$. It also turned out that the antioxidative activity of branch of S. nigra and K. japonica was similar to ${\alpha}$-tocopherol and butylated hydroxytoluene, one of the effective synthetic antioxidants. From these results, it can be suggested that the branches of S. nigra and K. japonica have the positive antioxidative activities and can be applied for the food preservatives and cosmetic ingredient.

• Korean Journal of Medicinal Crop Science
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• v.21 no.3
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• pp.165-173
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• 2013

The fruits of Schisandra chinensis have been used as an edible ingredient and traditional medicine in Korea. Due to morphological similarities of dried mature fruits, the correct identification of S. chinensis from other closely related Schisandrae species is very difficult. Therefore, molecular biological tools based on genetic analysis are required to identify authentic Schisandrae Fructus. Random amplifed polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop an easy, reliable and reproducible method for the authentication of these four species. In this paper, we developed several RAPD-derived species specific SCAR markers and established a multiplex-PCR condition suitable to discriminate each species. These genetic markers will be useful to distinguish and authenticate Schisandrae Fructus and four medicinal plants, S. chinensis, S. sphenanthera, S. repanda and K. japonica, in species level.