• Title/Summary/Keyword: KL-6

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Production of Fermented Feed from Food Wastes by Using Inoculation (종균 첨가에 의한 음식물 찌꺼기의 발효 사료화)

  • Suh, Eun-Hee;Song, Eun-Seung;Han, Uok;Lee, Sung-Taek;Yang, Jae-Kyung;Lee, Ki-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.5 no.1
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    • pp.1-13
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    • 1997
  • The fermentative conversion of food wastes into feed was investigated by seeding of mixed inoculum YM (Youngjin Environmental co.), and thermotolerant yeast Kl. marxianus. For 6 days' fermentaion, the fermentation method of 2 days' aerobic followed by 4 days' anaerobic was better for the production of organic acids and increasing total microbial population than 6 days' continuous aerobic or anaerobic fermentation. By seeding YM, the total microbial count increased about 100 times of the control group. In addition, Kl. marxianus seeding together with YM increased total viable cell count, but did not increase yeast count significantly.

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Investigating the potential exposure risk to indium compounds of target manufacturing workers through an analysis of biological specimens (생물학적 노출평가를 통한 타겟 제조업 근로자의 공정별 인듐 노출위험성 조사)

  • Won, Yong Lim;Choi, Yoon Jung;Choi, Sungyeul;Kim, Eun-A
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.24 no.3
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    • pp.263-271
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    • 2014
  • Objectives: Along with the several cases of pulmonary disorders caused by exposure to indium that have been reported in Japan, China, and the United States, cases of Korean workers involved in processes that require handling of indium compounds with potential risk of exposure to indium compounds have also been reported. We performed biological monitoring for workers in various target manufacturing processes of indium, indium oxide, and indium tin oxide(ITO)/indium zinc oxide(IZO) in domestic factories. Materials: As biological exposure indices, we measured serum concentrations of indium using inductively coupled plasma mass spectrometry, and Krebs von den Lungen 6(KL-6) and surfactant protein D(SP-D) using enzyme-linked immunosorbent assays. We classified the ITO/IZO target manufacturing process into powdering, mixing, molding, sintering, polishing, bonding, and finishing. Results: The powdering process workers showed the highest serum indium level. The mixing and polishing process workers also showed high serum indium levels. In the powdering process, the mean indium serum concentration in the workers exceeded $3{\mu}g/L$, the reference value in Japan. Of the powdering, mixing, and polishing process workers, 83.3%, 50.0%, and 24.5%, respectively, had values exceeding the reference value in Japan. We suppose that the reason of the higher prevalence of high indium concentrations in powder processing workers was that most of the particles in the powdering process were respirable dust smaller than $10{\mu}m$. The mean KL-6 and SP-D concentrations were high in the powdering, mixing, and polishing process workers. Therefore, the workers in these processes who were at greater risk of exposure to indium powder were those who had higher serum levels of indium, as well as KL-6 and SP-D. We observed significant differences in serum indium, KL-6, and SP-D levels between the process groups. Conclusions: Five among the seven reported cases of "indium lung" in Japan involved polishing process workers. Polishing process workers in Korea also had high serum levels of indium, KL-6, and SP-D. The outcomes of this study can be used as essential bases for establishing biological monitoring measures for workers handling indium compounds, and for developing health-care guidelines and special medical surveillance in Korea.

Structure Activity Relationship of Methylchroman-2-carboxylic Acid N-(Disubstituted)phenylamide Derivatives as Potential NF-${\kappa}B$ Inhibitors (메틸크로만-2-카르복실산 N-(이치환)페닐아마이드 유도체의 NF-${\kappa}B$ 저해 구조-활성 상관 관계)

  • Kim, Tae-Jeong;Kwak, Jae-Hwan;Kim, Young-Soo;Jung, Jae-Kyung;Lee, Hee-Soon
    • YAKHAK HOEJI
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    • v.55 no.2
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    • pp.154-159
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    • 2011
  • During the search for a novel compound that can inhibit NF-${\kappa}B$ activation, 6-hydroxy-7-methoxychroman-2-carboxylic acid phenyl amide (KL-1156) was identified as a good inhibitor of NF-${\kappa}B$ activation. In the present study, we describe the synthesis of methylchroman-2-carboxylic acid N-(disubstituted)phenylamide derivatives (1 and 2 serieses). In addition, their inhibitory effects of NF-${\kappa}B$ are compared with activity of KL-1156 and SAR (structure activity relationship) are explored.

Origin of the Cold Water below $10^{\circ}C$ Occurring in the Southern Coastal Region of the Korean East Sea in Summer by Ra Isotope Distribution (Ra동위체로 본 하계 동해 남부 연안해역에 출현하는 $10^{\circ}C$ 이하 냉수괴의 기원)

  • YANG Han-Soeb;KIM Pyoung-Joong;LEE Jae-Chul;MOON Chang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.4
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    • pp.404-412
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    • 1994
  • Radium isotopes were measured together with oceanographical parameters in the southern coastal region of the Korean East Sea during the period of September $2{\sim}8$, 1991. In September, there were various water masses vertically distributed in this region due to formation of strongly seasonal thermoclines. These water masses were characterized by activity of radium isotopes as well as water temperature and dissolved oxygen. Among the water masses, Japan Sea Proper Water(JSPW) below $1^{\circ}C$ had the highest Ra-226 activities but the lowest Ra-228 concentrations. However, Tsushima Surface Water (TSW) above $20^{\circ}C$ in water temperature had the highest Ra-228 which decreased sharply with depth. In TSW, Ra-228 activities were in the range of $194{\sim}270$ dpm/kl, which were approximately 10 times higher than JSPW. Activity ratios(A.R's) of Ra-228/Ra-226 were $1.9{\sim}2.6$ for TSW, $0.7{\sim}1.1$ for Tsushima Middle Water(TMW) of $12{\sim}17^{\circ}C\;to\;0.4{\sim}0.7$ for North Korea Cold Water(NKCW) with $1{\sim}7^{\circ}C$ and below 0.2 for JSPW. The Ra-228/Ra-226 ranged from 0.6 to 0.9 in the cold waters of $2{\sim}6^{\circ}C$, which were observed at depth of $65{\sim}120\;m$ in this study area. Radium isotopes provided a useful means of identifying origins of the cold water which occurred annually at intermediate or bottom layers in the southern coastal zone of the Korean East Sea. By plotting radium isotopes against water temperature, it could be observed clearly that the cold waters between $2{\sim}6^{\circ}C$ did not originate from the mixed water of JSPW and TMW but from NKCW.

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Physiological Characteristics of Starter Isolated from Kimchi and Fermentation of Tofu with Isolated Starter (발효두부 제조용 Starter의 선발과 이를 이용한 두부의 발효특성)

  • Kang, Kyoung Myoung;Lee, Shin Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.11
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    • pp.1626-1631
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    • 2012
  • Sixty strains of lactic acid bacteria were isolated from kimchi and used as a starter for fermented tofu. Among the isolated strains, strain KL-6 showed antimicrobial activity against various pathogens, antioxidative activity, and viability in artificial gastric juice and artificial bile acid. The selected strain KL-6 was identified as Pediococcus acidilactici KL-6 by morphological and physiological tests, including Gram staining, catalase test, and 16S rRNA sequencing. The fermentation characteristics of tofu with a kimchi ingredient mixture (Control) consisting of red pepper, garlic, ginger, sugar, salt, jeotgal, and juice of chinese cabbage were compared with those of tofus inoculated with strain KL-6 and the kimchi ingredient mixture (TL) or a pre-fermented kimchi ingredient mixture (TPL) for 24 hr at $37^{\circ}C$. The pH levels of all tested tofu samples decreased after 1 week of fermentation, reaching 3.96 (control), 3.97 (TL), and 4.03 log cfu/g (TPL) after fermentation for 14 weeks at $20^{\circ}C$. Total aerobe content of fermented tofu increased until 2 weeks of fermentation, but decreased steadily thereafter. The number of lactic acid bacteria reached $10^6$ cfu/g after 1 week of fermentation in TL and TPL, whereas it took 2 weeks for the control. The number of lactic acid bacteria in all tested tofu samples reached $10^3$ cfu/g after 14 weeks of fermentation at $20^{\circ}C$. Coliform bacteria were not detected in TL or TPL after 1 week of fermentation. The sensory scores of TL and TPL were higher than that of control in terms of taste, flavor, texture, and overall acceptability. The sensory quality of TPL was the best among all tested fermented tofu samples.

Studies of Plant Tumor Induction (Pat 1) Experiments on the Inoculation of Agrobacterium tumefaciens in out Field (식물의 암종유발에 관한 연구 1 (제 1 ) Agrobacterium tumefaciens 의 에 관하여)

  • 이민재;홍순우;최영길
    • Korean Journal of Microbiology
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    • v.4 no.2
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    • pp.1-4
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    • 1966
  • As a part of studies of plant tumor induction, this experiment was prepared for the purpose of studying the ability of tumor induction and the tendency of tumor initiation in some Korean plants using the various Agrobacterium tumefaciens strains. Results obtained from this experiment are as follows. The virulences of five strains used in this experiment were gradually decreased in order of strain A6Kl, B6, 11BV7, T37 and 11 BNV6. Especially strain T37 which is known to the host limited strain showed virulent effect to the most of plants given for the materials as well as strain A6Kl, B 6 and 11BV7. Concerning the grade of tumor development, in plants which has tough stem, for example, Glycine max Meer, tumor induction was not well developed after the inoculation of all strains. Particullary in Ricinus communes Linne all strains showed virulent effect but tumor tissues were declined in relation to the development of lignification. It was also confirmed that the induction of tumor tissues on plants is to delay according to the increase of the age of host plants.

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A SPECTRAL LINE SURVEY FROM 159.7 TO 164.7 GHZ TOWARD ORION-KL: THE DATA

  • LEE CHANG WON;CHO SE-HYUNG
    • Journal of The Korean Astronomical Society
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    • v.35 no.4
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    • pp.187-196
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    • 2002
  • A spectral line survey is performed from 159.7 to 164.7 GHz toward Orion-KL, as an extension of our previous line survey from 138.3 to 150.7 GHz with the same 14 m radio telescope of Taeduk Radio Astronomy Observatory. Typical system temperatures were 260 - 1000 K to achieve a sensitivity of about 0.02 - 0.04 K in TA unit. A total of 63 line spectra are detected in this survey. Among them, 54 lines lines are found to be the first detections towards an astronomical source and only 9 spectral lines have been previously identified from other observations. Forty-eight of 54 lines are believed to be from the known transitions of the known molecules, while 6 lines are 'unidentified'. All detected lines are found to be from a total of 10 molecular species and their isotopic variants. The molecular species with most numerous detected transitions are $HCOOCH_3$ (22), followed by $CH_3OCH_3$ (7), $C_2H_5CN$ (7), and $SO_2$ (6). The LTE rotation diagram analysis using all homogeneous data with those from previous survey gives more reliable determination of physical quantities. The derived values of the rotation temperatures and column densities for $HCOOCH_3$, $CH_3OCH_3$, and $SO_2$ are are 75 $\~$ 197 K and $1.5 {\~}18 {\times} 10^{15}\;cm^{-2}$, respectively.

Removal of Volatile Organic Compounds Using a Plasma Assisted Biotrickling System (플라즈마를 결합한 바이오 트리클링 시스템에 의한 휘발성 유기물질의 제거)

  • Kim, Hak-Joon;Han, Bang-Woo;Kim, Yong-Jin
    • Journal of Korean Society for Atmospheric Environment
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    • v.23 no.6
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    • pp.727-733
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    • 2007
  • In this study, a newly developed biotrickling system, combined with a non-thermal plasma reactor, was investigated to effectively treat gaseous contaminants such as VOCs (Volatile Organic Compounds). Three kinds of non-thermal plasmas (NTPs) such as a rod type dielectric barrier discharge (DBD) plasma, a packed bead type DBD plasma and a gliding arc (GA) plasma, were tested and compared in terms of power consumption. The rod type DBD plasma was selected as one for integration with biotrickling system due to its relatively high VOC removal efficiency, low power consumption and low pressure drop. Toluene and xylene as representatives of VOCs were used as test gases. The experiment results showed that the efficiency of biotrickling system was especially very low at the high gas concentration and high flow rate and the removal efficiencies of VOCs were considerably enhanced in the biotrickling system, when the DBD plasma was worked in front of that even at the high gas concentration and high flow rate.

Screening of Effective Components from Kale to Inhibit N - Nitrosodimethylamine Formation (케일로부터 N-Nitrosamine 생성을 억제시키는 유효성분의 검색)

  • 정미자;이수정;최선영;성낙주
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.2
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    • pp.223-229
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    • 2003
  • An amine rich diet with nitrate was incubated in simulated saliva, gastric juice, kale juice, and its ascorbate and methanol soluble portions (5, 10, 15 and 30 mL) for 1 hr at 37$^{\circ}C$ and N-nitrosodimethylamine (NDMA) was detected in the digestion sample, Kale juice and its ascorbate and methanol soluble portions at 30mL inhibited NDMA formation by 60.1$\pm$4.4%, 49.3$\pm$1.2% and 50.1$\pm$2.0%, respectively. The methanol soluble portion was further fractionated by preparative-LC (prep-LC). Nitrite-scavenging effects of 7 methanol soluble portion (Kl, K2, K3, K4, K5, K6 and K7) in kale juice were 2.0 ~56.2%. Among seven fractions, K3, K4, K5 and K7 exhibited weakly on nitrite scavenging effect. Fraction Kl and K2 inhibited NDMA formation by 71.0 and 65.5%, respectively. Fraction Kl and K2 was further separated by prep-LC into 6 subfractions (Kla, Klb, Klc, K2a, K2b and 2nc). Those subfractions inhibited NDMA formation by 40.9 ~80.4%. The K2a subfraction was screened by MS, $^1$H-NMR, $^{13}$ C-NMR and DEPT spectrum.