• The Korea Journal of Herbology
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• v.28 no.4
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• pp.63-69
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• 2013

Objectives : The purpose of present study was to investigate the vasorelaxant activities and mechanisms of action of the ethanol extract of P. yedoensis leaf (PYL) on isolated rat aortic rings. Methods : Dried P. yedoensis leaves were extracted 3 times with 100% ethanol for 3 h in a reflux apparatus. Isolated rat aortic rings were suspended in organ chambers containing 10 ml Krebs-Henseleit (K-H) solution. The rings were maintained at $37^{\circ}C$ and aerated with a mixture of 95% $O_2$ and 5% $CO_2$. Changes in their tension were recorded via isometric transducers connected to a data acquisition system. Results : PYL relaxed the contraction of aortic rings induced by phenylephrine (PE, 1 ${\mu}M$) or KCl (60 mM) in a concentration dependent manner. However, the vasorelaxant effects of PYL on endothelium-denuded aortic rings were lower than endothelium-intact aortic rings. And the vasorelaxant effects of PYL on endothelium-intact aortic rings were reduced by pre-treatment with $N{\omega}$-Nitro-L-arginine methyl ester (10 ${\mu}M$), methylene blue (10 ${\mu}M$), 1-H-[1,2,4]-oxadiazolo-[4,3-${\alpha}$]-quinoxalin-1-one (10 ${\mu}M$), tetraethylammonium (5 mM). In addition, PYL inhibited the contraction induced by extracellular $Ca^{2+}$ in endothelium-denuded aortic rings pre-contracted by PE or KCl in $Ca^{2+}$-free K-H solution. Conclusions : These results suggest that PYL exerts its vasorelaxant effects via the activation of Nitric Oxide (NO) formation by means of L-arginine and NO-cGMP pathways and via the blockage of receptor operated calcium channels, voltage dependent calcium channels and calcium-activated potassium channels.

• Journal of Ginseng Research
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• v.42 no.2
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• pp.165-174
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• 2018

Background: Extended endoplasmic reticulum (ER) stress may initiate apoptotic pathways in cancer cells, and ER stress has been reported to possibly increase tumor death in cancer therapy. We previously reported that caspase-8 played an important role in compound K-induced apoptosis via activation of caspase-3 directly or indirectly through Bid cleavage, cytochrome c release, and caspase-9 activation in HL-60 human leukemia cells. The mechanisms leading to apoptosis in A549 and SK-MES-1 human lung cancer cells and the role of ER stress have not yet been understood. Methods: The apoptotic effects of compound K were analyzed using flow cytometry, and the changes in protein levels were determined using Western blot analysis. The intracellular calcium levels were monitored by staining with Fura-2/AM and Fluo-3/AM. Results: Compound K-induced ER stress was confirmed through increased phosphorylation of $eIF2{\alpha}$ and protein levels of GRP78/BiP, XBP-1S, and $IRE1{\alpha}$ in human lung cancer cells. Moreover, compound-K led to the accumulation of intracellular calcium and an increase in m-calpain activities that were both significantly inhibited by pretreatment either with BAPTA-AM (an intracellular $Ca^{2+}$ chelator) or dantrolene (an RyR channel antagonist). These results were correlated with the outcome that compound K induced ER stress-related apoptosis through caspase-12, as z-ATAD-fmk (a specific inhibitor of caspase-12) partially ameliorated this effect. Interestingly, 4-PBA (ER stress inhibitor) dramatically improved the compound K-induced apoptosis. Conclusion: Cell survival and intracellular $Ca^{2+}$ homeostasis during ER stress in human lung cancer cells are important factors in the induction of the compound K-induced apoptotic pathway.

• The Journal of Korean Academy of Prosthodontics
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• v.43 no.3
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• pp.352-362
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• 2005

Statement of problem. Accurate impression is essential to success of implant prostheses. But there have been few studies about the accuracy of fixture-level impression techniques in internal connection implant systems. Purpose. The purpose of this study was to compare the accuracy of two fixture-level impression techniques in two conditions (parallel and divergent) and to assess the effect of tightening sequences and forces on stresses generated on superstructures in internal connection implant system (Astra Tech). Material and methods. Two metal master frameworks made from two abutments (Cast-to Abutment ST) each and a corresponding, passively fitting, dental stone master cast with four fixture replicas (Fixture Replica ST) were fabricated. Ten dental stone casts for each impression techniques (direct unsplinted & splinted technique) were made with vinyl polysiloxane impressions from the master cast. Strain gauges for each framework were fixed midway between abutments to measure the degree of framework deformation on each stone cast. Pairs of strain gauges placed opposite each other constituted one channel (half Wheatstone bridge) to read deformation in four directions (superior, inferior, anterior, and posterior). Deformation data were analyzed using one-way ANOVA and the Tukey test at the .01 level of significance. And the effect of tightening sequences (right-to-left and left-to-right) and forces (10 Ncm and 20 Ncm) were assessed with ten stone casts made from parallel condition by the splinted technique. Deformation data were analyzed using paired t-test at the .01 level of significance. Conclusions. Within the limitations of this study, the following conclusions could be drawn. 1. Frameworks bent toward the inferior side on all casts made by both direct unsplinted and splinted impression techniques in both parallel and divergent conditions. 2. There was no statistically significant difference of accuracy between the direct unsplinted and splinted impression techniques in both parallel and divergent conditions (P>.01). 3. There was no statistically significant difference of stress according to screw tightening sequences in casts made by the splinted impression technique in parallel condition (P>.01). 4. Greater tightening force resulted in greater stress in casts made by the splinted impression technique in parallel condition (P<.01).

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.172.2-172.2
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• 2010

This study was carried out in order to reduce the installation expense of heating system for greenhouse comparing to geothermal heat pump and develope the coefficient of performance (COP) for a heat pump. For getting plenty of heat flux from geothermal energy. Surface water in river channel was used for getting a lots of geothermal heat by penetrating water through underground soil layer of the river bank that make heat transmission to passing water. The range of water temperature after the process of Ground filtration is 13~18 degrees celsius which is very similar to low heat source of geothermal heat pump system and the plenty amount of heat source from that make the number of geothermal heat exchanging hole and the expense for geothermal heat exchanger construction reduced. Drainage well is also used for returning filtration water to the aquifer that keep the water good recirculation from losing geothermal heat and water resource. For the COP improvement of Heat pump, thermal storage tank with separating insulation plate according to the temperature difference make the COP of Heat pump that is similar to thermal storage tank with diffuser. Developed thermal storage tank make construction expense cheaper than customarily used one's. and that sand filter and oxidation sand (FELOX) are going to be used for improving ground filtration water quality that make heat exchanger efficiency better. All above developed component skill are going to be set on the Ground filtration water source heat pump system and applied for medium, large scale for protected greenhouse in riverside area and on-site experiment is going to do for optimizing the heating system function and overcome the problem happening in the process of on-site application afterward.

• Atmosphere
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• v.15 no.1
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• pp.35-46
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• 2005

Today, the outreach of National Meteorological Service such as PC cluster based Numerical Weather Prediction (NWP) technique is vigorous in the world wide. In this regard, WMO (World Meteorological Organization) asked KMA (Korea Meteorological Administration) to formulate a regional project, which cover most of RA II members, using similar technical system with KMA's. In that sense, Meteorological Research Institute (METRI) in KMA developed Mongolian NWP System (MNWPS) based on PC cluster and transferred the technology to Weather Service Center in Mongolia. The hybrid parallel algorithm and channel bonding technique were adopted to cut cost and showed 41% faster performance than single MPI (Message Passing Interface) approach. The cluster technique of Beowulf type was also adopted for convenient management and saving resources. The Linux based free operating system provide very cost effective solution for operating multi-nodes. Additionally, the GNU software provide many tools, utilities and applications for construction and management of a cluster. A flash flood event happened in Mongolia (2 September 2003) was selected for test run, and MNWPS successfully simulated the event with initial and boundary condition from Global Data Assimilation and Prediction System (GDAPS) of KMA. Now, the cluster based NWP System in Mongolia has been operated for local prediction around the region and provided various auxiliary charts.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.20 no.9
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• pp.868-882
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• 2009

In this paper, channel model and wireless link performance analysis for the short-range wireless communication system applications in the terahertz frequency which is currently interested in many countries will be described. In order to realize high data rates above 10 Gbps, the more wide bandwidths will be required than the currently available bandwidths of millimeter-wave frequencies, therefore, the carrier frequencies will be pushed to THz range to obtain larger bandwidths. From the THz atmospheric propagation characteristics based on ITU-R P.676-7, the available bandwidths were calculated to be 68, 48 and 45 GHz at the center frequencies of 220, 300 and 350 GHz, respectively. With these larger bandwidths, it was shown from the simulation that higher data rate above 10 Gbps can be achieved using lower order modulation schemes which have spectral efficiency of below 1. The indoor propagation delay spread characteristics were analyzed using a simplified PDP model with respect to building materials. The RMS delay spread was calculated to be 9.23 ns in a room size of $6\;m(L){\times}5\;m(W){\times}2.5\;m(H)$ for the concrete plaster with TE polarization, which is a similar result of below 10 ns from the Ray-Tracing simulation in the reference paper. The indoor wireless link performance analysis results showed that receiver sensitivity was $-56{\sim}-46\;dBm$ over bandwidth of $5{\sim}50\;GHz$ and antenna gain was calculated to be $26.6{\sim}31.6\;dBi$ at link distance of 10m under the BPSK modulation scheme. The maximum achievable data rates were estimated to be 30, 16 and 12 Gbps at the carrier frequencies of 220, 300 and 350 GHz, respectively, under the A WGN and LOS conditions, where it was assumed that the output power of the transmitter is -15 dBm and link distance of 1 m with BER of $10^{-12}$. If the output power of transmitter is increased, the more higher data rate can be achieved than the above results.

• Korean Journal of Digital Imaging in Medicine
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• v.11 no.2
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• pp.51-57
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• 2009

The purpose of this study would evaluate if having clinical effects on diffusion image with quantitative analysis through ADC values of brain's normal tissue and lesions before and after contrast injections using a 3.0T. From November in 2007 until December in 2008, a total of 32 patient was performed on 3.0T(Signa Excite, GE Medical System, USA) with the normal or lesions in the patient who requests diffusion weighted image with 8channel head coil. The pulse sequence was used with spin echo EPI(TR: 10000msec, TE: 72.2 msec, Matrix: 128*128, FOV: 240 mm, NEX: 1, diffusion direction: 3, b-value: 1000). Measurement results of ADC values on lesions, CSF, white matter, gray matter, lesions after contrast injection were measured less 75% than before contrast injection, infarction: 100%, CSF: 78%(high), white matter: 71.4%(low), gray matter: 50%(high, low). The results of paired t-test on the deference of ADC values which statically is significant in three(lesions, CSF, white matter)regions except for white matter(p<0.05). Quantitative analysis of lesions, CSF, white matter, gray matter have difference on all regions. ADC values were low in lesions and white matter, normal CSF after contrast injection commonly is high than before contrast injection, ADC values which white matter were high and low (50:50) after contrast injection. 3.0T diffusion weighted image clinically supposed that performing DWI examination after contrast injection was not desirable because of having effects on brain tissue.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.34 no.3
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• pp.274-282
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• 1998

This study deals with sinking resistance for purse seine, in the case of different d/l, ratio of twine diameter and leg length. Experiments carried out on the six types simplified reduced model seines which were made of knotless netting. The nettings were woven in different leg length 4.3, 5.0, 5.5, 6.0, 6.6 and 7.7mm of polyester 28 tex two threads two -ply twine, and each of the seines were named I, II, III, IV, V and Ⅵ seine. Dimension of seine models were 450cm for corkline and 85cm for seine depth, each seines rigged up 160g of float for a floatline and 50g (underwater weight) of lead for a leadline. Experiments were measured in the observation channel of a flume tank at the static conditions Sinking motion was recorded by the two sets TV-camera for VTR which were placed in top and side of the model seine, and reading coordinate carried out by the video digitization system. An analysis were calculated out by simultaneous differential equations for numerical method by Runge - Kutta - Gill sub - routine. The results obtained were as follows: 1. Average sinking speed of seine of seine margin was fastest for Ⅵ seine followed by V, IV, III, II and I seines. 2. The coefficient of resistance for a seine wall was depended upon the ratio of d/l : KD =0.081 (d/l )-0.5 3. The coefficient of resistance for netting bundle was not depended upon the ratio of d/l :CR = 0.91 (), d : Twine diameter, l : Leg length, : Density of netting materals, $\omega$ : Density of water

• Proceedings of KOSOMES biannual meeting
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• 2007.05a
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• pp.161-168
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• 2007

In this paper, the floating body with double barriers is introduced with a study on the flow patterns and characteristics in around the floating body by using 2 frame p article tracking method. This paper introduce an analisys method to predict the characteristics of flow around the neighbording fields of Floating Body with double barriers in order to investigate a high performance model. Flow visualization has conducted in a circulating water channel by a high speed camera and etc. Flow phenomena according to velocity distribution and flow separation around the floating body with double barriers were obtained by two-dimensional PIV system.

• Journal of Microbiology and Biotechnology
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• v.23 no.1
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• pp.125-130
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• 2013

Influenza viruses cause significant morbidity and mortality in humans through epidemics or pandemics. Currently, two classes of anti-influenza virus drugs, M2 ion-channel inhibitors (amantadin and rimantadine) and neuraminidase inhibitors (oseltamivir and zanamivir), have been used for the treatment of the influenza virus infection. Since the resistance to these drugs has been reported, the development of a new antiviral agent is necessary. In this study, we examined the antiviral efficacy of the plant extracts against the influenza A/PR/8/34 infection. In vitro, the antiviral activities of the plant extracts were investigated using the cell-based screening. Three plant extracts, Thuja orientalis, Aster spathulifolius, and Pinus thunbergii, were shown to induce a high cell viability rate after the infection with the influenza A/PR/8/34 virus. The antiviral activity of the plant extracts also increased as a function of the concentration of the extracts and these extracts significantly reduced the visible cytopathic effect caused by virus infections. Furthermore, the treatment with T. orientalis was shown to have a stronger inhibitory effect than that with A. spathulifolius or P. thunbergii. These results may suggest that T. orientalis has anti-influenza A/PR/8/34 activity.