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http://dx.doi.org/10.6116/kjh.2013.28.4.63.

Vasorelaxant Effect of Prunus yedoensis leaf on Rat Aortic Rings  

Lee, Kyungjin (Department of Herbology, College of Korean Medicine, Kyung Hee University)
Kim, Kwang-Woo (Department of Herbology, College of Korean Medicine, Kyung Hee University)
Heo, Heeseung (Department of Herbology, College of Korean Medicine, Kyung Hee University)
Ham, Inhye (Department of Herbology, College of Korean Medicine, Kyung Hee University)
Lee, Mi-Hwa (Department of Herbology, College of Korean Medicine, Kyung Hee University)
Kim, Bumjung (Department of Herbology, College of Korean Medicine, Kyung Hee University)
Bu, Youngmin (Department of Herbology, College of Korean Medicine, Kyung Hee University)
Kim, Hocheol (Department of Herbology, College of Korean Medicine, Kyung Hee University)
Choi, Ho-Young (Department of Herbology, College of Korean Medicine, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.28, no.4, 2013 , pp. 63-69 More about this Journal
Abstract
Objectives : The purpose of present study was to investigate the vasorelaxant activities and mechanisms of action of the ethanol extract of P. yedoensis leaf (PYL) on isolated rat aortic rings. Methods : Dried P. yedoensis leaves were extracted 3 times with 100% ethanol for 3 h in a reflux apparatus. Isolated rat aortic rings were suspended in organ chambers containing 10 ml Krebs-Henseleit (K-H) solution. The rings were maintained at $37^{\circ}C$ and aerated with a mixture of 95% $O_2$ and 5% $CO_2$. Changes in their tension were recorded via isometric transducers connected to a data acquisition system. Results : PYL relaxed the contraction of aortic rings induced by phenylephrine (PE, 1 ${\mu}M$) or KCl (60 mM) in a concentration dependent manner. However, the vasorelaxant effects of PYL on endothelium-denuded aortic rings were lower than endothelium-intact aortic rings. And the vasorelaxant effects of PYL on endothelium-intact aortic rings were reduced by pre-treatment with $N{\omega}$-Nitro-L-arginine methyl ester (10 ${\mu}M$), methylene blue (10 ${\mu}M$), 1-H-[1,2,4]-oxadiazolo-[4,3-${\alpha}$]-quinoxalin-1-one (10 ${\mu}M$), tetraethylammonium (5 mM). In addition, PYL inhibited the contraction induced by extracellular $Ca^{2+}$ in endothelium-denuded aortic rings pre-contracted by PE or KCl in $Ca^{2+}$-free K-H solution. Conclusions : These results suggest that PYL exerts its vasorelaxant effects via the activation of Nitric Oxide (NO) formation by means of L-arginine and NO-cGMP pathways and via the blockage of receptor operated calcium channels, voltage dependent calcium channels and calcium-activated potassium channels.
Keywords
Prunus yedoensis; vasorelaxant; vascular endothelium; hypertension; calcium channel;
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Times Cited By KSCI : 1  (Citation Analysis)
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