• Biomolecules & Therapeutics
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• 제30권4호
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• pp.348-359
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• 2022

Gastric adenocarcinoma is among the top causes of cancer-related death and is one of the most commonly diagnosed carcinomas worldwide. Benzyl isothiocyanate (BITC) has been reported to inhibit the gastric cancer metastasis. In our previous study, BITC induced apoptosis in AGS cells. The purpose of the present study was to investigate the effect of BITC on autophagy mechanism in AGS cells. First, the AGS cells were treated with 5, 10, or 15 μM BITC for 24 h, followed by an analysis of the autophagy mechanism. The expression level of autophagy proteins involved in different steps of autophagy, such as LC3B, p62/SQSTM1, Atg5-Atg12, Beclin1, p-mTOR/mTOR ratio, and class III PI3K was measured in the BITC-treated cells. Lysosomal function was investigated using cathepsin activity and Bafilomycin A1, an autophagy degradation stage inhibitor. Methods including qPCR, western blotting, and immunocytochemistry were employed to detect the protein expression levels. Acridine orange staining and omnicathepsin assay were conducted to analyze the lysosomal function. siRNA transfection was performed to knock down the LC3B gene. BITC reduced the level of autophagy protein such as Beclin 1, class III PI3K, and Atg5-Atg12. BITC also induced lysosomal dysfunction which was shown as reducing cathepsin activity, protein level of cathepsin, and enlargement of acidic vesicle. Overall, the results showed that the BITC-induced AGS cell death mechanism also comprises the inhibition of the cytoprotective autophagy at both initiation and degradation steps.

• 한국강구조학회 논문집
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• 제26권4호
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• pp.263-275
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• 2014

본 연구에서는 SRC 기둥-H형단면과 U형단면으로 구성된 합성보 접합부에 관해서 반복가력실험을 실시하였다. 합성보에 관한 핵심요소는 H형단면과 U형단면 간 용접접합부의 구조적 성능이다. 이 두 부재 접합부의 성능을 향상시키기 위해서는 수직스티프너와 사다리꼴 스티프너가 필요하다. 접합부의 반복적인 성능을 평가하기 위해서 5개의 실대형 실험체를 계획하였으며, 실험변수는 H형단면의 크기($H-500{\times}200{\times}10{\times}16$, $H-600{\times}200{\times}11{\times}17$), 스티프너와 상부근의 유무 및 용접접근공(WAH)의 유무 등이다. 실험결과, H-500 시리즈 및 H-600 시리즈 보가 있는 실험체의 회전각은 각각 4%와 3%로 나타났으며, 이는 특수모멘트골조와 중간모멘트골조에 요구되는 값이다. 실험결과는 스티프너와 상부근이 있는 실험체의 변형능력이 그렇지 않은 실험체에 비해서 우수한 결과를 보였다. 끝으로, 실험체의 에너지소산능력과 변형도 분포를 요약하였다.

• 보건의료산업학회지
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• 제4권2호
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• pp.33-46
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• 2010

In this study, we investigated web contents accessibility of 60 hospital web sites in Korea. The eight evaluation criteria were used for estimating the web contents accessibility of the web sites. These criteria were as follows: providing an alternative text, providing caption for moving picture, providing a skip navigation, usage of pop-up windows, usage of a summary or a caption tag for data table, providing a page title, providing a label for online form, and usage of java scripts. K-WAH 3.0 was used for estimating five evaluation criteria. According to Internet web contents accessibility guideline 1.0, we estimated the rest three evaluation criteria manually and described good or bad examples for the evaluation results technically. The results show that the web accessibility of hospital web sites is generally insufficient and the constant interests in improvement for accessibility are urgently needed.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2016년도 춘계 종합학술대회 논문집
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• pp.41-42
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• 2016

본 연구는 국내 주요 연구기관 사이트를 대상으로 웹 접근성 실태 조사를 하였다. 연구기관의 구체적인 웹 접근성 준수 실태 파악을 위해 자동화 평가도구인 K-WAH 4.0을 사용하였다. 평가 결과 국내의 20여개의 연구기관 중 6개의 연구기관만이 웹 접근성 마크를 획득하였으며 획득한 연구기관의 준수율은 96%, 획득하지 못한 연구기관의 준수율은 86%, 전체 평균 준수율 89%로 접근성 마크를 획득한 연구기관이 획득하지 못한 연구기관에 비하여 다소 높은 것으로 나타났지만 웹 접근성마크를 받은 연구기관 중 웹 접근성을 100% 준수하는 사이트를 발견하지 못하였다. 이에 본 연구에서는 연구기관 사이트 중 잘 지켜지지 않은 항목들을 파악한 후 국내 연구기관 웹 사이트의 접근성 수준 향상에 기여하고자 한다.

• Journal of Acupuncture Research
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• 제19권4호
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• pp.89-100
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• 2002

Objective : We want to have a correct understanding and the distance about the Oriental Medical therapy on the CP(cerebral palsy) children. Methods : We made up a question of parents of CP children and analyzed it. Conclusion : 19 patients were taken physical treatment and 12 patients were taken occupational treatment in company with physical treatment(In our OPD CP patient) Parents complained about poor surroundings of our hospital for children and deficiency of physical treatment. The most satisfactory thing is acupuncture treatment, then the next thing is aroma therapy. On the satisfaction degree about the Oriental Medical therapy, very good is 27.3%, good 45.5%, ordinary 27.3% and unsatisfaction is none. The place Oriental medical therapy before our hospital was taken is local Oriental medical clinic, 9 cases out of 22.

• ETRI Journal
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• 제42권4호
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• pp.619-632
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• 2020

Highly dispersive S-boxes are desirable in cryptosystems as nonlinear confusion sublayers for resisting modern attacks. For a near optimal cryptosystem resistant to modern cryptanalysis, a highly nonlinear and low differential probability (DP) value is required. We propose a method based on a piecewise linear chaotic map (PWLCM) with optimization conditions. Thus, the linear propagation of information in a cryptosystem appearing as a high DP during differential cryptanalysis of an S-box is minimized. While mapping from the chaotic trajectory to integer domain, a randomness test is performed that justifies the nonlinear behavior of the highly dispersive and nonlinear chaotic S-box. The proposed scheme is vetted using well-established cryptographic performance criteria. The proposed S-box meets the cryptographic performance criteria and further minimizes the differential propagation justified by the low DP value. The suitability of the proposed S-box is also tested using an image encryption algorithm. Results show that the proposed S-box as a confusion component entails a high level of security and improves resistance against all known attacks.

• Child Studies in Asia-Pacific Contexts
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• 제7권1호
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• pp.15-26
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• 2017

The aim of this study was to retrospectively estimate the prevalence of childhood emotional abuse (CEA), childhood physical abuse (CPA), and childhood contact sexual abuse (CCSA) in relation to adult poor mental health, addictive behavior, and other health-risk behaviors among university students in five ASEAN countries (Indonesia, Malaysia, Myanmar, Thailand, and Vietnam). In a cross-sectional survey, 3,240 undergraduate university students were randomly selected (M age = 20.5 years, SD = 1.6 years) to respond to a questionnaire including the Abuse section of the World Health Organization (WHO) Version 1 "Adverse Childhood Experiences International Questionnaire" (ACE-IQ) and other measures. The students reported 17.9% CEA, 28.2% CPA, and 22.4% CCSA, with the highest prevalence of CEA in Myanmar (30.9%) and CPA and CCSA in Vietnam (55.8% and 41.6%, respectively). In logistic regression models, adjusting for sociodemographic and social variables, the separate and cumulative effects of three types of child abuse (emotional, physical, and sexual) were found to increase the risks for poor adult mental health, addictive, and other health-risk behaviors.

• 셀메드
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• 제4권4호
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• pp.28.1-28.27
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• 2014

Linderae Radix, the dry roots of Lindera aggregata (Sims) Kosterm, has long been used as traditional Chinese medicine for treatment of inflammatory diseases. The total alkaloids are believed to be the active components responsible for anti-inflammation of Linderae Radix. Here, the total alkaloids of Linderae Radix were extracted and isolated, including 12 isoquinoline alkaloids and 1 furan sesquiterpene. Within the alkaloids, norisoboldine, boldine, linderaline, isoboldine, reticuline, N-methyllaurotetanine, norjuziphine were found to be the major ingredients. In lipopolysaccharide-treated macrophage RAW 264.7 cells, application of Linderae Radix extract, or total alkaloids, suppressed the transcription of proinflammatory cytokines, interleukin-$1{\beta}$ and interleukin-6. Out of the 12 alkaloids, norisoboldine, boldine, and isoboldine were tested in lipopolysaccharide-treated macrophages, and norisoboldine was the strongest alkaloid in suppressing the cytokine expressions. The current studies suggested that the identification of alkaloids from Linderae Radix could provide a plausible explanation for herbal therapeutic functions.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.1925-1931
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• 2016

Background: The XRCC1 protein facilitates various DNA repair pathways; single-nucleotide polymorphisms (SNPs) in this gene are associated with a risk of gastrointestinal cancer (GIC) with inconsistent results, but no data have been previously reported for the Sabah, North Borneo, population. We accordingly investigated the XRCC1 Arg194Trp and Arg399Gln SNPs in terms of GIC risk in Sabah. Materials and Methods: We performed genotyping for both SNPs for 250 GIC patients and 572 healthy volunteers using a polymerase chain reaction-restriction fragment length polymorphism approach. We validated heterozygosity and homozygosity for both SNPs using direct sequencing. Results: The presence of a variant 194Trp allele in the Arg194Trp SNP was significantly associated with a higher risk of GIC, especially with gastric and colorectal cancers. We additionally found that the variant 399Gln allele in Arg399Gln SNP was associated with a greater risk of developing gastric cancer. Our combined analysis revealed that inheritance of variant alleles in both SNPs increased the GIC risk in Sabah population. Based on our etiological analysis, we found that subjects ${\geq}50years$ and males who carrying the variant 194Trp allele, and Bajau subjects carrying the 399Gln allele had a significantly increased risk of GIC. Conclusions: Our findings suggest that inheritance of variant alleles in XRCC1 Arg194Trp and Arg399Gln SNPs may act as biomarkers for the early detection of GIC, especially for gastric and colorectal cancers in the Sabah population.

• 감성과학
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• 제12권4호
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• pp.481-488
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• 2009

웹 2.0이 등장하면서 나타난 개념인 SNS(Social Network Service 또는 Social Network Sites)는 오프라인에서 만 존재하던 사회적 관계망을 온라인에서 구축한 친목기반 인맥 형성 서비스이다. 페이스북(Facebook), 마이스페이스(Myspace), 트위터(Twiter)등과 같은 외국 SNS와 싸이월드(Cyworld), 미투데이(Me2Day)등과 같은 국내 SNS는 지속적인 인기를 누리며, 인터넷 환경의 주요한 응용 분야로 자리 잡고 있다. 이러한 SNS는 모든 사용자들이 타인과 협력하고 정보를 공유하는 것을 목적으로 하고 있기 때문에, 웹을 사용하는데 어려움을 가지고 있는 노인과 장애인들이 사용하기에 적절한 인터페이스를 제공하고 있는지 웹 접근성을 평가할 필요가 있다. 웹 접근성은 인지적, 신체적 특징에 상관없이 모든 사람에게 동등한 웹 기회를 제공하고 있는가를 나타내는 지표이다. 만약, SNS가 웹 접근성이 낮아 노인과 장애인이 사용하지 못한다면, 노인, 장애인과 일반사용자의 정보격차는 점점 늘어나서 사회적 문제가 될 것이다. 이에 본 논문에서는 웹 접근성 평가 도구인 KADO-WAH 2.0과 웹 접근성 표준 문서인 WCAG(Web Contents Accessibility Guideline) 2.0을 기반으로 SNS인 페이스북과 싸이월드의 웹 접근성을 평가하여, 웹 접근성 준수 현황을 파악하고, 개선방향을 제시하고자 한다.