• Biomolecules & Therapeutics
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• v.30 no.4
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• pp.348-359
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• 2022

Gastric adenocarcinoma is among the top causes of cancer-related death and is one of the most commonly diagnosed carcinomas worldwide. Benzyl isothiocyanate (BITC) has been reported to inhibit the gastric cancer metastasis. In our previous study, BITC induced apoptosis in AGS cells. The purpose of the present study was to investigate the effect of BITC on autophagy mechanism in AGS cells. First, the AGS cells were treated with 5, 10, or 15 μM BITC for 24 h, followed by an analysis of the autophagy mechanism. The expression level of autophagy proteins involved in different steps of autophagy, such as LC3B, p62/SQSTM1, Atg5-Atg12, Beclin1, p-mTOR/mTOR ratio, and class III PI3K was measured in the BITC-treated cells. Lysosomal function was investigated using cathepsin activity and Bafilomycin A1, an autophagy degradation stage inhibitor. Methods including qPCR, western blotting, and immunocytochemistry were employed to detect the protein expression levels. Acridine orange staining and omnicathepsin assay were conducted to analyze the lysosomal function. siRNA transfection was performed to knock down the LC3B gene. BITC reduced the level of autophagy protein such as Beclin 1, class III PI3K, and Atg5-Atg12. BITC also induced lysosomal dysfunction which was shown as reducing cathepsin activity, protein level of cathepsin, and enlargement of acidic vesicle. Overall, the results showed that the BITC-induced AGS cell death mechanism also comprises the inhibition of the cytoprotective autophagy at both initiation and degradation steps.

• Journal of Korean Society of Steel Construction
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• v.26 no.4
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• pp.263-275
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• 2014

In this study, connection of steel reinforced concrete(SRC) column and composite beam which consists of H-section and U-section members were tested under cyclic loading. An essential point of the composite beam is the structural performance of welded joint between the H-section and the U-section members. To improve the structural performance of joint of two beam members, vertical stiffeners, trapezoidal stiffeners, and top bars were used. Five full-scaled specimens were designed to study the effect of a number of parameters on cyclic performance of connections such as H-section beam size($H-500{\times}200{\times}10{\times}16$, $H-600{\times}200{\times}11{\times}17$), the presence of stiffeners and top bars, and the presence of no weld access hole(WAH) method. Based on the test results, deformation capacity of the specimens with H-500 series beam and H-600 series beam were 4% and 3% rotation angle, which is the requirement for the Special Moment Frame and Intermediate Moment Frame(IMF), respectively. Test result showed that deformation capacity of connection with stiffeners and top bars is greater than that of connection without stiffeners and top bars. Finally, energy dissipation capacity and strain profile of specimens were summarized.

• The Korean Journal of Health Service Management
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• v.4 no.2
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• pp.33-46
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• 2010

In this study, we investigated web contents accessibility of 60 hospital web sites in Korea. The eight evaluation criteria were used for estimating the web contents accessibility of the web sites. These criteria were as follows: providing an alternative text, providing caption for moving picture, providing a skip navigation, usage of pop-up windows, usage of a summary or a caption tag for data table, providing a page title, providing a label for online form, and usage of java scripts. K-WAH 3.0 was used for estimating five evaluation criteria. According to Internet web contents accessibility guideline 1.0, we estimated the rest three evaluation criteria manually and described good or bad examples for the evaluation results technically. The results show that the web accessibility of hospital web sites is generally insufficient and the constant interests in improvement for accessibility are urgently needed.

• Proceedings of the Korea Contents Association Conference
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• 2016.05a
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• pp.41-42
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• 2016

본 연구는 국내 주요 연구기관 사이트를 대상으로 웹 접근성 실태 조사를 하였다. 연구기관의 구체적인 웹 접근성 준수 실태 파악을 위해 자동화 평가도구인 K-WAH 4.0을 사용하였다. 평가 결과 국내의 20여개의 연구기관 중 6개의 연구기관만이 웹 접근성 마크를 획득하였으며 획득한 연구기관의 준수율은 96%, 획득하지 못한 연구기관의 준수율은 86%, 전체 평균 준수율 89%로 접근성 마크를 획득한 연구기관이 획득하지 못한 연구기관에 비하여 다소 높은 것으로 나타났지만 웹 접근성마크를 받은 연구기관 중 웹 접근성을 100% 준수하는 사이트를 발견하지 못하였다. 이에 본 연구에서는 연구기관 사이트 중 잘 지켜지지 않은 항목들을 파악한 후 국내 연구기관 웹 사이트의 접근성 수준 향상에 기여하고자 한다.

• Journal of Acupuncture Research
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• v.19 no.4
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• pp.89-100
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• 2002

Objective : We want to have a correct understanding and the distance about the Oriental Medical therapy on the CP(cerebral palsy) children. Methods : We made up a question of parents of CP children and analyzed it. Conclusion : 19 patients were taken physical treatment and 12 patients were taken occupational treatment in company with physical treatment(In our OPD CP patient) Parents complained about poor surroundings of our hospital for children and deficiency of physical treatment. The most satisfactory thing is acupuncture treatment, then the next thing is aroma therapy. On the satisfaction degree about the Oriental Medical therapy, very good is 27.3%, good 45.5%, ordinary 27.3% and unsatisfaction is none. The place Oriental medical therapy before our hospital was taken is local Oriental medical clinic, 9 cases out of 22.

• ETRI Journal
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• v.42 no.4
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• pp.619-632
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• 2020

Highly dispersive S-boxes are desirable in cryptosystems as nonlinear confusion sublayers for resisting modern attacks. For a near optimal cryptosystem resistant to modern cryptanalysis, a highly nonlinear and low differential probability (DP) value is required. We propose a method based on a piecewise linear chaotic map (PWLCM) with optimization conditions. Thus, the linear propagation of information in a cryptosystem appearing as a high DP during differential cryptanalysis of an S-box is minimized. While mapping from the chaotic trajectory to integer domain, a randomness test is performed that justifies the nonlinear behavior of the highly dispersive and nonlinear chaotic S-box. The proposed scheme is vetted using well-established cryptographic performance criteria. The proposed S-box meets the cryptographic performance criteria and further minimizes the differential propagation justified by the low DP value. The suitability of the proposed S-box is also tested using an image encryption algorithm. Results show that the proposed S-box as a confusion component entails a high level of security and improves resistance against all known attacks.

• Child Studies in Asia-Pacific Contexts
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• v.7 no.1
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• pp.15-26
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• 2017

The aim of this study was to retrospectively estimate the prevalence of childhood emotional abuse (CEA), childhood physical abuse (CPA), and childhood contact sexual abuse (CCSA) in relation to adult poor mental health, addictive behavior, and other health-risk behaviors among university students in five ASEAN countries (Indonesia, Malaysia, Myanmar, Thailand, and Vietnam). In a cross-sectional survey, 3,240 undergraduate university students were randomly selected (M age = 20.5 years, SD = 1.6 years) to respond to a questionnaire including the Abuse section of the World Health Organization (WHO) Version 1 "Adverse Childhood Experiences International Questionnaire" (ACE-IQ) and other measures. The students reported 17.9% CEA, 28.2% CPA, and 22.4% CCSA, with the highest prevalence of CEA in Myanmar (30.9%) and CPA and CCSA in Vietnam (55.8% and 41.6%, respectively). In logistic regression models, adjusting for sociodemographic and social variables, the separate and cumulative effects of three types of child abuse (emotional, physical, and sexual) were found to increase the risks for poor adult mental health, addictive, and other health-risk behaviors.

• CELLMED
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• v.4 no.4
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• pp.28.1-28.27
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• 2014

Linderae Radix, the dry roots of Lindera aggregata (Sims) Kosterm, has long been used as traditional Chinese medicine for treatment of inflammatory diseases. The total alkaloids are believed to be the active components responsible for anti-inflammation of Linderae Radix. Here, the total alkaloids of Linderae Radix were extracted and isolated, including 12 isoquinoline alkaloids and 1 furan sesquiterpene. Within the alkaloids, norisoboldine, boldine, linderaline, isoboldine, reticuline, N-methyllaurotetanine, norjuziphine were found to be the major ingredients. In lipopolysaccharide-treated macrophage RAW 264.7 cells, application of Linderae Radix extract, or total alkaloids, suppressed the transcription of proinflammatory cytokines, interleukin-$1{\beta}$ and interleukin-6. Out of the 12 alkaloids, norisoboldine, boldine, and isoboldine were tested in lipopolysaccharide-treated macrophages, and norisoboldine was the strongest alkaloid in suppressing the cytokine expressions. The current studies suggested that the identification of alkaloids from Linderae Radix could provide a plausible explanation for herbal therapeutic functions.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1925-1931
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• 2016

Background: The XRCC1 protein facilitates various DNA repair pathways; single-nucleotide polymorphisms (SNPs) in this gene are associated with a risk of gastrointestinal cancer (GIC) with inconsistent results, but no data have been previously reported for the Sabah, North Borneo, population. We accordingly investigated the XRCC1 Arg194Trp and Arg399Gln SNPs in terms of GIC risk in Sabah. Materials and Methods: We performed genotyping for both SNPs for 250 GIC patients and 572 healthy volunteers using a polymerase chain reaction-restriction fragment length polymorphism approach. We validated heterozygosity and homozygosity for both SNPs using direct sequencing. Results: The presence of a variant 194Trp allele in the Arg194Trp SNP was significantly associated with a higher risk of GIC, especially with gastric and colorectal cancers. We additionally found that the variant 399Gln allele in Arg399Gln SNP was associated with a greater risk of developing gastric cancer. Our combined analysis revealed that inheritance of variant alleles in both SNPs increased the GIC risk in Sabah population. Based on our etiological analysis, we found that subjects ${\geq}50years$ and males who carrying the variant 194Trp allele, and Bajau subjects carrying the 399Gln allele had a significantly increased risk of GIC. Conclusions: Our findings suggest that inheritance of variant alleles in XRCC1 Arg194Trp and Arg399Gln SNPs may act as biomarkers for the early detection of GIC, especially for gastric and colorectal cancers in the Sabah population.

• Science of Emotion and Sensibility
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• v.12 no.4
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• pp.481-488
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• 2009

The SNS(Social Network Service or Social Network Sites), the concept which came along with the Web2.0 is on-line service which focuses on building social network communities of people which used to be established off-line. Foreign SNS sites such as Facebook, MySpace and Twitter and domestic SNS sites such as Cyworld, Me2Day are getting popularity continuously and became one of the most important application in internet environments. Since these SNS sites have the purpose of providing infrastructure which enable all the people to share his/her interests with all the other people, it is necessary to appraise whether these sites provide convenient interfaces with proper accessibility to handicapped people and old people who are considered having difficulties in using internet. Web accessibility refers to the practice of providing equal access to people of all abilities and disabilities. If the web accessibility of a SNS site is so low that handicapped people and old people cannot use it properly, those gaps between user groups may become social problems. Therefore, the paper appraised web accessibility of Facebook and Cyworld using automatic tool, KADO-WAH 2.0 and Standard guideline, WCAG 2.0 and suggested the improvement opportunities based on the results of the appraisal.