• Journal of the Computational Structural Engineering Institute of Korea
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• v.33 no.2
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• pp.121-128
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• 2020

The purpose of this study is to investigate the distribution patterns of displacement and acceleration fields in a nonlinear soil ground based on the interaction of high-speed train, wheel, rail, and ground. For this purpose, a high-speed train in motion was modeled as the actual wheel, and the vertical contact of wheel and rail and the lateral contact, caused by meandering motion, were simulated; this simulation was based on the moving mass analysis. The soil ground part was given the nonlinear behavior of the upper ground part by using the modified the Drucker-Prager model, and the changes in displacement and acceleration were compared with the behavior of the elastic and inelastic grounds. Using this analysis, the displacement and acceleration ranges close to the actual ground behavior were addressed. Additionally, the von-Mises stress and equivalent plastic strain at the ground were examined. Further, the equivalent plastic and total volumetric strains at each failure surface were examined. The variation in stresses, such as vertical stress, transverse pressure, and longitudinal restraint pressure of wheel-rail contact, with the time history was investigated using moving mass. In the case of nonlinear ground model, the displacement difference obtained based on the train travel is not large when compared to that of the elastic ground model, while the acceleration is caused to generate a large decrease.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.37 no.1
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• pp.1-7
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• 2017

In general, the large loads which are applied from explosion, impact, earthquake and wind at a short time caused the materials of structures to large deformations, rotations and strains locally. If such phenomena will be analyzed, hydrocodes which can be considered fluid-structure interaction under computational continuum mechanics are inevitably needed. Also, the explosion mechanism is so complicated, it is reasonable that the behaviors of structure are predicted through explosion analyses and experiment at the same time. But, unfortunately, it is true that explosion experiments are limited to huge cost, large experiment facilities and safety problems. Therefore, in this study, it is shown that the results of explosion analyses using the AUTODYN are agreed with those of existing explosion experiments for reinforced concrete slabs within reasonable error limits. And the explosion damage of the same reinforced concrete slab are assessed for quite different reinforcement arrangement spacings, concrete cover depths, and vertical reinforcements. From the explosion analyses, it is known that the more the ratio of slab thickness to reinforcement arrangement spacing is increased, and small-diameter reinforcements are used than large-diameter reinforcements on the same reinforcement ratio, and vertical reinforcements are used, the more the anti-knock capacities are improved.

• Steel and Composite Structures
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• v.13 no.3
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• pp.239-258
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• 2012

In this paper, experimental investigations on high strength steel (HSS) stud connected steel-concrete composite (SCC) girders to understand the effect of shear connector density on their flexural behaviour is presented. SCC girder specimens were designed for three different shear capacities (100%, 85%, and 70%), by varying the number of stud connectors in the shear span. Three SCC girder specimens were tested under monotonic/quasi-static loading, while three similar girder specimens were subjected to non-reversal cyclic loading under simply supported end conditions. Details of casting the specimens, experimental set-up, and method of testing, instrumentation for the measurement of deflection, interface-slip and strain are discussed. It is found that SCC girder specimen designed for full shear capacity exhibits interface slip for loads beyond 25% of the ultimate load capacity. Specimens with lesser degree of shear connection show lower values of load at initiation of slip. Very good ductility is exhibited by all the HSS stud connected SCC girder specimens. It is observed that the ultimate moment of resistance as well as ductility gets reduced for HSS stud connected SCC girder with reduction in stud shear connector density. Efficiency factor indicating the effectiveness of high strength stud connectors in resisting interface forces is estimated to be 0.8 from the analysis. Failure mode is primarily flexure with fracturing of stud connectors and characterised by flexural cracking and crushing of concrete at top in the pure bending region. Local buckling in the top flange of steel beam was also observed at the loads near to failure, which is influenced by spacing of studs and top flange thickness of rolled steel section. One of the recommendations is that the ultimate load capacity can be limited to 1.5 times the plastic moment capacity of the section such that the post peak load reduction is kept within limits. Load-deflection behaviour for monotonic tests compared well with the envelope of load-deflection curves for cyclic tests. It is concluded from the experimental investigations that use of HSS studs will reduce their numbers for given loading, which is advantageous in case of long spans. Buckling of top flange of rolled section is observed at failure stage. Provision of lips in the top flange is suggested to avoid this buckling. This is possible in case of longer spans, where normally built-up sections are used.

• Journal of Mushroom
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• v.6 no.3_4
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• pp.131-137
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• 2008

These experiments were conducted to search for the optimum media size and composition for culture of Lentinula edodes (JNM3022). Considering the incubation period and the successful culturing rate, the optimum media size was length 25 $cm{\times}diameter$ 14 cm (weight 2.5 kg). The saturation moisture amount of Oak sawdust was 251 ml/100 g. It is a half of pine sawdust 495 ml/100 g. Considering the hypha growing speed and hypha density, the optimum media composition was Oak sawdust 80%+ wheat bran 20% and Oak sawdust 80%+ rice bran 20% In 10 treatments of media composition. The optimum moisture amount of media for growing Lentinula edodes (JNM3022) was 55%. Injection pipe with 20 holes in media was reduced the incubation periods to 11 days when 20cm length media and 32 days when 30cm length media compare to control. The best method for browning media surface was change temperature condition above $10^{\circ}C$ and punching the vinyl bag. This treat could shortened the browning periods to 54 days.

• Elastomers and Composites
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• v.36 no.2
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• pp.111-120
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• 2001

Mechanical properties and their adhesion behavior with zinc- and brass-plated steel cords of natural rubber/acrylonitrile-butadiene blend compounds were investigated as a function of blend ratio. The Mooney viscosity and stress relaxation time were found to be lowered with increasing NBR content. Tensile modulus generally increased with increasing NBR content. Tensile stress at break stayed constant up to about 40 phr and showed minimum at $50{\sim}60 phr$, and thereafter increased with increasing NBR content. Strain at break decreased linearly below 50 phr, and above the level it showed nearly constant value. Based on the abrupt drops in elastic modulus and tan ${\delta}$ peak, the glass transition temperature of NR and NBR were found to be -55 and $-10^{\circ}C$, respectively. In the case of NR/NBR blend compounds, two distinct transition points were observed and each transition position was not affected by NBR level indicating an incompatible nature of NR/NBR blend system. The pullout force and rubber coverage decreased to the level of about 40% to that of pure m compound, when the 50 phr of NR was replaced by NBR. However, the pure NBR compound showed the comparable adhesion performance with NR(${\sim}90%$). The sulfur concentration was found to become lower with the increased NBR content at the adhesion interface based on the Auger spectrometer results, representing a lack of adhesion layer formation, and this was explained for a possible cause of low adhesion performance with adding NBR.

• Clinical and Experimental Reproductive Medicine
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• v.31 no.3
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• pp.155-168
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• 2004

Objective: Melatonin, which is secreted by pineal gland play an important role in the regulation of ovarian function via seasonal rhythm and sleep in most mammals. It also has a role in the protection of cells by removing toxic oxygen free radicals brought about by metabolism. In the present study, effects of melatonin on the mouse oocyte maturation were examined using two different culture conditions provided with 5% or 21% oxygen concentration. Material and Method: Immature mouse oocytes were obtained from the ovarian follicles of $3{\sim}4$ weeks old ICR strain mice intraperitoneally injected with 5 I.U. PMSG 44 hour before. Under stereomicroscope, morphologically healthy oocytes with distinct germinal vesicle (GV) were liberated from the graafian follicles and collected using mouth-controlled micropipette. They were then cultured for 17 hour at $37^{circ}C$, 5% $CO_2$ and 21% $O_2$ (95% air) or 5% $CO_2$, 5% $O_2$ and 90% $N_2$. New modified Hank's balanced salt solution (New MHBS) was used as a culture medium throughout the experiments. Effects of melatonin were examined at a concentration of $0.0001{\mu}M$, $0.01{\mu}M$ or $1.0{\mu}M$. For the prevention of spontaneous maturation of immature oocytes during culture, dibutyryl cyclic AMP (dbcAMP) and/or hypoxanthine were included in the medium. Results: Under 21% oxygen condition, oocytes cultured in the presence of $0.01{\mu}M$ melatonin showed a significantly higher maturation rates, in terms of germinal vesicle breakdown (95.0% vs 89.0%) and polar body formation (88.1% vs 75.4%), compared to those cultured with $0.0001{\mu}M$ or $1.0{\mu}M$ melatonin. However, no difference was observed in oocytes cultured under 5% oxygen whether they were treated with melatonin or not. In the presence of $0.01{\mu}M$ melatonin, oocytes either cultured under 21% or 5% oxygen exhibited no difference in the polar body formation (85.6% vs 86.7%). However, in the absence of melatonin, oocytes cultured under 21% oxygen exhibited lower polar body formation (74.7%). When oocytes were cultured in the presence of dbcAMP alone or with varying concentrations of melatonin, those treated with both compounds always showed better maturation, i.e., germinal vesicle breakdown and polar body formation, compared to those cultured with dbcAMP alone. At the same concentration of melatonin, however, oocytes exposed to 21% oxygen showed poor maturation than those to 5% oxygen. Similar results were obtained from the experiments using hypoxanthine instead of dbcAMP. Conclusion: Based upon these results, it is suggested that melatonin could enhance the meiotic maturation of mouse oocytes under 21% oxygen concentration, and release oocytes from the meiotic arrest by dbcAMP or hypoxanthine regardless of the concentration of oxygen, probably via the removal of oxygen free radicals.

• Journal of Adhesion and Interface
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• v.18 no.1
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• pp.13-24
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• 2017

In this study, nanoparticles which encapsulated hydrophobic antimicrobial compounds with 50wt% of payload and 70%of solid content were prepared. These nanoparticles could be dispersed at water as well as various medium. Water dispersible organic-inorganic (O-I) hybrid nanoparticles were first prepared using alkoxysilane-functionalized amphiphilic polymer precursors through a conventional sol-gel process. Hydrophobic antimicrobial compound, Eugenol encapsulated nanoparticles were prepared using these O-I hybrid nanoparticles through a new nanoprecipitation process. The effect of various preparation on the size of nanoparticles, amount of payload, antimicrobial activity, and release rate of encapsulated compounds was investigated. All eugenol-encapsulated O-I nanoparticles regardless of preparation condition showed the same minimal inhibitory concentration (MIC) (50mg/ml) and 99% of antimicrobial activity for every strain. Their antimicrobial activity could maintain longer than two weeks. Especially, eugenol-encapsulated O-I nanoparticles prepared using tetraethoxysilane (TEOS) exhibited the highest payload (50wt%) and the lowest release rate which was owing to higher inorganic content in the O-I nanoparticles. And these O-I nanoparticles dispersed in hexanediol (HD) showed the highest antimicrobial activity and solid content (70wt%) because HD acted as a solvent as well as a antimicrobial agent.

• Korean Journal of Microbiology
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• v.52 no.2
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• pp.148-156
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• 2016

This study examined effects of Enterobacter ludwigii SJR3 showing a high 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, on growth of tomato plant and its expression of stress-related genes under drought and salt stress. SJR3 strain was inoculated at $10^6cell/g$ soil to 4-week grown tomato plants, and drought and salt stresses were treated. After additional incubation for 1 week, root length, stem length, fresh weight and dry weight of tomato plants treated with SJR3 increased by 37.8, 37.2, 96.8 and 146.6%, respectively compared to those of uninoculated plants in drought stress environment, and they increased by 19.2, 25.4, 19.5, and 105.8%, respectively in salt stress environment. Proline content in tomato leaves increased significantly under stress conditions as one of a protecting substance against stresses, but proline contents in tomato treated with SJR3 decreased by 62.1 and 54.1%, respectively. Relative expression of genes encoding ACC oxidase, ACO1 and ACO4, ethylene response factor genes ERF1 and ERF4, and some other stress-related genes were examined from tomato leaves. Compared to the non-stressed tomato, expressions of all stress-related genes increased significantly in the stressed tomato, but gene expressions in the inoculated tomato were similar to those of no-stressed control tomato. Therefore, E. ludwigii SJR3 may play an important role in mitigating drought and salt stress in plants, and can increase productivity of crops under various abiotic stresses.

• Korean Journal of Microbiology
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• v.50 no.4
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• pp.302-307
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• 2014

Many rhizobacteria can promote plant growth through various direct or indirect mechanisms, and their production of phytohormones such as indole-3-acetic acid (IAA) may have pronounced effects on growth and development of plants. Rhizobacterial strain isolated from rhizosphere of foxtail (Setaria viridis), Acinetobacter sp. SW5 produced 118.1 mg/L of IAA and 4.5 mg/L of gibberellin ($GA_3$) in brain heart broth medium at 2 and 1 day of incubation, respectively. In a pot test the lengths of stem and root and fresh weight of the germinated tomato seedlings treated with Acinetobacter sp. SW5 significantly increased by 26.3, 33.3, and 105.3%, respectively compared to those of the uninoculated control in 12 weeks of cultivation. When the root exudate secreted from tomato seedlings was analyzed by HPLC, 3.75 ng mg tomato $root^{-1}$ of tryptophan which is an IAA precursor was detected. Acinetobacter sp. SW5 could produce $4.06{\mu}M$ of IAA from root exudate from 8 tomato seedlings. Together with the capability of growth of Acinetobacter sp. SW5 in the tomato root exudates, this IAA secreted by bacteria might contribute to enhance the growth of tomato plants.

• Journal of Biomedical Engineering Research
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• v.18 no.3
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• pp.273-283
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• 1997

Electrogastrography(EGG), the cutaneous recording of the myoelectrical activity of the stomach using surface electrodes, is attactive due to its non-invasiveness. Despite many attempts made over the decades, analysis of surface EGG has not led to identification of contraction-related electrical response activity of the stomach that would help the clinician to diagnose motility disorders of the stomach. We propose feasible methods to detect the gastric contraction by spectrum analysis and vector analysis of the surface EGG signal. A running spectral analysis(RSA) based on the fast Fourier transform (FFT) was applied to the filtered EGG signal. The powers of dominant frequency and its harmonics were compared with gastric contraction signals such as the strain gauge signal from the gastric serosa in dog or the antropyloric pressure in human. And we also carried out vector analysis of the filtered EGG signals obtained from three paired electrodes. The amplitude and direction of the calculated EGG vector were analyzed and compared with the gastric contraction signals. From the spectrum analysis, we found that the increase of the power of the first harmonic of the dominant frequency was highly correlated with the gastric contraction. And from the vector analysis of the EGG signal, we found a typical change of the amplitude and direction of the EGG vector, which can indicate occurrences of the gastric contraction.