• Korean Journal of Veterinary Research
• /
• v.39 no.2
• /
• pp.247-256
• /
• 1999

A disease of young Holstein calves characterized by recurrent pneumonia, ulcerative and granulomatous stomatitis, enteritis with bacterial overgrowth, periodontitis, delayed wound healing, persistent neutrophilia and death at an early age had been originally described in 1983 and again in 1987. Most of these calves had stunted growth and a persistent, progressive neutrophilia (often exceeding 100,000/ml). By investigation of pedigrees, all of the affected calves have now been traced to a common sire and confirmed by polymerase chain reaction (PCR) diagnostic DNA testing to be homozygous carriers of a defective allele for bovine CD18. Neutrophils from these calves have several functional deficits and, most importantly, fail to adhere in a ${\beta}_2$-integrin dependent manner. The ${\beta}_2$-integrins represent a family of glycoproteins which participate in various leukocyte adhesion reactions during host defense. The presence or absence of ${\beta}_2$-integrin molecules can be demonstrated on the surface of neutrophils, monocytes and lymphocytes from normal or affected calves using specific monoclonal antibodies and flow cytometry, or by colloidal gold immunolabeling and scanning electron microscopy in backscatter mode. Deficiency of the ${\beta}_2$-integrins on all leukocyte types in Holstein calves is analogous to leukocyte adhesion deficiency (LAD) seen in humans. Neutrophils in bovine (BLAD) and human LAD patients are unable to adhere to the endothelial lining of the cardiovascular system thus interrupting egression of neutrophils into infected tissues. Other leukocytes, while still deficient in expression of the ${\beta}_2$-integrins, are still able to efficiently egress from the blood stream due to interactions of other adhesion molecules that are not as highly expressed on neutrophils. Both BLAD cattle and LAD children (who do not receive bone marrow transplants) often die at an early age as a result of the failure of neutrophils to extravasate into infected tissues. In 1991, Shuster, et $al^{27}$, identified two point mutations within the alleles encoding bovine CD18 in a Holstein calf afflicted with leukocyte adhesion deficiency. One mutation causes an aspartic acid to glycine substitution at amino acid 128 (D128G) in an extracellular region of this adhesion glycoprotein that is highly conserved (> 95% identity) between humans, cattle and mice. The other mutation is silent. Numerous calves with clinical symptoms of leukocyte adhesion deficiency have since been tested and all have been found homozygous for the D128G allele. In addition, calves homozygous far the D128G allele have been identified during widespread DNA testing in the United States. All cattle with the mutant allele are related to one bull, who through artificial insemination (A.I.), sired many calves in the 1950's and 1960's. The carrier frequency of the D128G CD18 allele among U.S. Holstein cattle had reached approximately 15% among active A.I. bulls and 8% among cows. By 1993, the organization of the dairy industry and the diagnostic test developed to genotype cattle, enabled virtually complete eradication of bovine leukocyte adhesion deficiency among current and future A.I. bulls.

• Food Science of Animal Resources
• /
• v.37 no.1
• /
• pp.1-9
• /
• 2017

The rotavirus-induced diarrhea of human and animal neonates is a major public health concern worldwide. Until recently, no effective therapy is available to specifically inactivate the rotavirion particles within the gut. Passive immunotherapy by oral administration of chicken egg yolk antibody (IgY) has emerged of late as a fresh alternative strategy to control infectious diseases of the alimentary tract and has been applied in the treatment of diarrhea due to rotavirus infection. The purpose of this concise review is to evaluate evidence on the properties and performance of anti-rotavirus immunoglobulin Y (IgY) for prevention and treatment of rotavirus diarrhea in human and animal neonates. A survey of relevant anti-rotavirus IgY basic studies and clinical trials among neonatal animals (since 1994-2015) and humans (since 1982-2015) have been reviewed and briefly summarized. Our analysis of a number of rotavirus investigations involving animal and human clinical trials revealed that anti-rotavirus IgY significantly reduced the severity of clinical manifestation of diarrhea among IgY-treated subjects relative to a corresponding control or placebo group. The accumulated information as a whole depicts oral IgY to be a safe and efficacious option for treatment of rotavirus diarrhea in neonates. There is however a clear need for more randomized, placebo controlled and double-blind trials with bigger sample size to further solidify and confirm claims of efficacy and safety in controlling diarrhea caused by rotavirus infection especially among human infants with health issues such as low birth weights or compromised immunity in whom it is most needed.

• Korean Journal of Social Welfare
• /
• v.35
• /
• pp.85-105
• /
• 1998

This article examined the trend of 125 empirical researches which were published in Jr. of Korean Social Welfare from the first issue to no. 33. in terms of theoretical and methodological orientations. The content analysis was employed for the purpose of the study. Since 1979, the number of empirical researches was in the trend of increasing. The findings from this research were as follows. 1) Among 166 authors, 96.4% were majored in social welfare. Also 6.0% were practitioners and the rest of them were in the position of professors or researchers. The outcome of lack of interdisciplinary co-work and researcher-practitioner co-work led the article to conclude that the nature of applied social science of social welfare was not so actively pursued in Korea. 2) It was almost impossible to find researches which studied same theme or employed same analytical framework. This meant that the work of re-verifying and proving the contray could not be done although it was essential for theory-building. In other words, the disciplinary of social welfare was far behind in the process of theory-building. 3) The methodology upon which most of researches were relied was quantitative methodology(92.8%). The article concluded 'paradigm shift' was not begun in the disciplinary of social welfare yet. 4) The study concluded that the particularity of empirical researches of social welfare in Korea was descriptive-configurative study. Whereas 65.5% of 125 empirical studies were descriptive-configurative, 25% were hypothesis - model test and only 6% causal analysis. 5) The most applied statistic models through the period from 1979 to 1997 were descriptive statistics such as frequency, chi square test, Pearson's r. More advanced statistics such as logit regression, probit regression, path analysis, covariance structure analysis were shown since 1990.

• Journal of Veterinary Clinics
• /
• v.27 no.1
• /
• pp.62-65
• /
• 2010

Arowana (Scleropages formosus) is the most valuable group of ornamental fishes and very much in demand in the ornamental fish trade and commands high price ranging from hundreds to thousands of dollars per fish. In this paper, we described a case of mortality of arowana from a private aquarium in Korea. A bacterial pathogen from fish organs (brain, kidney, liver) was cultured, identified and confirmed using Vitek System 2, API 20E test, multiplex PCR and 16S rRNA gene sequencing. The morphological and biochemical properties of the bacterium isolated from the brain, kidney and liver of the fish were similar to Aeromonas sobria. Positive amplification products using the multiplex PCR assay for detection of A. sobria were obtained from these organs. The 16S rRNA gene of the isolates from fish was identical and exhibited 100% sequence similarity with A. sobria (AY987762.1) strain available from GenBank. This bacterium contained hemolysin gene, a virulence factor that plays an important role in outbreaks of disease and is pathogenic to humans as well as in fish. Although this opportunistic bacterium was isolated from a fish without any external symptoms, this pathogen may act as a reservoir and enhance chances of zoonosis to human such as during handling.

• Journal of Veterinary Clinics
• /
• v.27 no.5
• /
• pp.618-621
• /
• 2010

A giant grouper (Epinephelus lanceolatus) that was reared for public exhibition in a private commercial aquarium in Seoul, Korea, was recently found dead. The fish had evidenced symptoms including anorexia, lethargy, and depression persisting for two weeks. A bacterial pathogen from fish organs (kidney, liver, spleen) was cultured, identified and confirmed as Photobacterium damselae subsp. damselae, using a Vitek System 2, API 20E test, multiplex PCR, and 16S rRNA gene sequencing. In this paper, we have described the isolation and identification of Photobacterium damselae subsp. damselae from a giant grouper reared in a private aquarium in Korea.

• Korean Journal of Food Science and Technology
• /
• v.33 no.6
• /
• pp.802-807
• /
• 2001

Effects of ${\beta}-glucan$ from Lentinus edodes and hordeum vulgare on blood glucose and lipid composition were investigated. Diabetes mellitus was induced in male ICR mice by the injection of alloxan into the tail vein at a dose of 75 mg/kg. The ${\beta}-glucan$ were administered orally for 10 days and the normal and alloxan-control group were orally administered with saline. The body weight gain and food intake were monitored every day and plasma levels of glucose, triglyceride, total cholesterol, LDL-cholesterol were determined at last day. Also the weight of liver, heart, spleen and kidney were determined. The ${\beta}-glucan$ from Lentinus edodes and hordeum vulgure lowered significantly body weight gain in alloxan-induced diatetic mice (p<0.05) and plasma glucose levels compared to that of alloxan-control group. Plasma triglyceride level in B500 was lowered in alloxan-induced diabetic mice. The ${\beta}-glucan$ of hordeum vulgare lowered weight of liver significantly (p<0.05). In conclusion, it was assumed that ${\beta}-glucan$ from hordeum vulgare have anti-hyperglycemic and anti-obese effects by reducing body weight gain and decreasing serum glucose and triglyceride level.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.6 no.2
• /
• pp.63-71
• /
• 2002

This paper examines the ASCE first generation benchmark problem for a seismically excited cable-stayed bridge, and proposes a new semi-active control strategy focusing on inclusion of effects of control-structure interaction. This benchmark problem focuses on a cable-stayed bridge in Cope Girardeau, Missouri, USA, for which construction is expected to be completed in 2003. Seismic considerations were strongly considered in the design of this bridge due to the location of the bridge in the New Madrid seismic zone and its critical role as a principal crossing of the Mississippi River. In this paper, magnetorheological(MR) fluid dampers are proposed as the supplemental damping devices, and a clipped-optimal control algorithm is employed. Several types of dynamic models for MR fluid dampers, such as a Bingham model, a Bouc-Wen model, and a modified Bouc-Wen model, are considered, which are obtained from data based on experimental results for full-scale dampers. Because the MR fluid damper is a controllable energy-dissipation device that cannot add mechanical energy to the structural system, the proposed control strategy is fail-safe in that bounded-input, bounded-output stability of the controlled structure is guaranteed. Numerical simulation results show that the performance of the proposed semi-active control strategy using MR fluid dampers is quite effective.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.8 no.3
• /
• pp.63-76
• /
• 2004

This paper presents LRB-based hybrid base isolation systems employing additional active/semiactive control devices for mitigating earthquake-induced vibration of a cable-stayed 29 bridge. Hybrid base isolation systems could improve the control performance compared with the passive type-base isolation system such as LRB-installed bridge system due to multiple control devices are operating. In this paper, the additional response reduction by the two typical additional control devices, such as active type hydraulic actuators controlled by LQG algorithm and semiactive-type magnetorheological dampers controlled by clipped-optimal algorithm, have been evaluated bypreliminarily investigating the slightly modified version of the ASCE phase I benchmark cable-stayed bridge problem (i.e., the installation of LRBs to the nominal cable-stayed bridge model of the problem). It shows from the numerical simulation results that all the LRB based hybrid seismic isolation systems considered are quite effective to mitigate the structural responses. In addition, the numerical results demonstrate that the LRB based hybrid seismic isolation systems employing MR dampers have the robustness to some degree of the stiffness uncertainty of in the structure, whereas the hybrid system employing hydraulic actuators does not. Therefore, the feasibility of the hybrid base isolation systems employing semiactive additional control devices could be more appropriate in realfor full-scale civil infrastructure applications is clearly verified due to their efficacy and robustness.

• Proceedings of the KSR Conference
• /
• 2006.11b
• /
• pp.1017-1028
• /
• 2006

Japanese railroad companies continued growing by developing diversification based on a railroad enterprise. However, after entering in the 1990s, the diversification model of a railroad company reached the management limit. Under economic depression, A decrease in the birthrate and aging progressed and passenger transport changed to the downward tendency. Nevertheless, since railroad investment was expanded, railroad achievements got worse and price competitiveness with JR East Japan became weak. But the achievements of a diversification section got worse compared with the railroad enterprise. Therefore, group management was thought as important and enterprise reorganization was developed.

• Journal of Pharmacopuncture
• /
• v.19 no.3
• /
• pp.207-212
• /
• 2016

Objectives: Acupuncture treatments are safe and effective for a wide variety of diseases involving autonomic dysregulation. Heart rate variability (HRV) is a noninvasive method for assessing sympathovagal balance. The low frequency/high frequency (LF/HF) spectral power ratio is an index of sympathovagal influence on heart rate and of cardiovascular health. This study tests the hypothesis that from rest to 30% to 50% of peak oxygen consumption, the nonlinear Conte-Zbilut-Federici (CZF) method of computing the LF/HF ratio is a more reliable index of changes in the HRV than linear methods are. Methods: The subjects of this study were 10 healthy young adults. Electrocardiogram RR intervals were measured during 6-minute periods of rest and aerobic exercise on a cycle ergometer at 30% and 50% of peak oxygen consumption ($VO_{2peak}$). Results: The frequency domain CZF computations of the LF/HF ratio and the time domain computations of the standard deviation of normal-to-normal intervals (SDNN) decreased sequentially from rest to 30% $VO_{2peak}$ (P < 0.001) to 50% $VO_{2peak}$ (P < 0.05). The SDNN and the CZF computations of the LF/HF ratio were positively correlated (Pearson's r = 0.75, P < 0.001). fast Fourier transform (FFT), autoregressive (AR) and Lomb periodogram computations of the LF/HF ratio increased only from rest to 50% $VO_{2peak}$. Conclusion: Computations of the LF/HF ratio by using the nonlinear CZF method appear to be more sensitive to changes in physical activity than computations of the LF/HF ratio by using linear methods. Future studies should determine whether the CZF computation of the LF/HF ratio improves evaluations of pharmacopuncture and other treatment modalities.