Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Isolation of Aeromonas sobria Containing Hemolysin Gene from Arowana (Scleropages formosus)

Arowana(Scleropages formosus)에서 Hemolysin Gene을 지닌 Aeromonas sobria 분리 및 특성

  • Jun, Jin-Woo (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Ji-Hyung (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Casiano, Choresca Jr. (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Dennis, K. Gomez (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Shin, Sang-Phil (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Han, Jee-Eun (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Park, Se-Chang (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University)
  • 전진우 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 김지형 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 카시아노 허모피아 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 데니스 고메즈 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 신상필 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 한지은 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 박세창 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단)
  • Published : 2010.02.28
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Abstract

Arowana (Scleropages formosus) is the most valuable group of ornamental fishes and very much in demand in the ornamental fish trade and commands high price ranging from hundreds to thousands of dollars per fish. In this paper, we described a case of mortality of arowana from a private aquarium in Korea. A bacterial pathogen from fish organs (brain, kidney, liver) was cultured, identified and confirmed using Vitek System 2, API 20E test, multiplex PCR and 16S rRNA gene sequencing. The morphological and biochemical properties of the bacterium isolated from the brain, kidney and liver of the fish were similar to Aeromonas sobria. Positive amplification products using the multiplex PCR assay for detection of A. sobria were obtained from these organs. The 16S rRNA gene of the isolates from fish was identical and exhibited 100% sequence similarity with A. sobria (AY987762.1) strain available from GenBank. This bacterium contained hemolysin gene, a virulence factor that plays an important role in outbreaks of disease and is pathogenic to humans as well as in fish. Although this opportunistic bacterium was isolated from a fish without any external symptoms, this pathogen may act as a reservoir and enhance chances of zoonosis to human such as during handling.

Keywords

References

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