Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Isolation of Photobacterium Damselae Subsp. Damselae from the Giant Grouper, Epinephelus Lanceolatus

Giant Grouper (Epinephelus Lanceolatus)에서 Photobacterium Damselae subsp. Damselae 분리 및 특성

  • Jun, Jin-Woo (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Ji-Hyung (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Han, Jee-Eun (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Shin, Sang-Phil (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Gomez, Dennis K. (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Casiano, Choresca Jr. (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Oh, Kyu-Seon (Basic Research Laboratory, Center for Cancer Research, National Cancer Institute) ;
  • Park, Se-Chang (Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • 전진우 (서울대학교 수의과대학 수생동물질병학연구실) ;
  • 김지형 (서울대학교 수의과대학 수생동물질병학연구실) ;
  • 한지은 (서울대학교 수의과대학 수생동물질병학연구실) ;
  • 신상필 (서울대학교 수의과대학 수생동물질병학연구실) ;
  • 데니스 고메즈 (서울대학교 수의과대학 수생동물질병학연구실) ;
  • 카시아노 허모피아 (서울대학교 수의과대학 수생동물질병학연구실) ;
  • 오규선 (미국 국립보건원 암연구센터) ;
  • 박세창 (서울대학교 수의과대학 수생동물질병학연구실)
  • Accepted : 2010.09.16
  • Published : 2010.10.30
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Abstract

A giant grouper (Epinephelus lanceolatus) that was reared for public exhibition in a private commercial aquarium in Seoul, Korea, was recently found dead. The fish had evidenced symptoms including anorexia, lethargy, and depression persisting for two weeks. A bacterial pathogen from fish organs (kidney, liver, spleen) was cultured, identified and confirmed as Photobacterium damselae subsp. damselae, using a Vitek System 2, API 20E test, multiplex PCR, and 16S rRNA gene sequencing. In this paper, we have described the isolation and identification of Photobacterium damselae subsp. damselae from a giant grouper reared in a private aquarium in Korea.

서울 소재 수족관에서 관상용으로 사육되었던 giant grouper (Epinephelus lanceolatus) 한 마리가 폐사된 채발견되었다. 폐사어는 2주간 식욕부진, 무기력 및 침울의 증상을 보였다. 폐사어의 장기 (신장, 간 및 비장)로부터 세균을 순수 분리하여 Vitek System 2, API 20E test, multiplex PCR 및 16S rRNA gene sequencing을 수행하여 Photobacterium damselae subsp. damselae로 확진하였다. 본 증례에서, 우리는 국내 수족관에서 사육되었던 giant grouper에서의 Photobacterium damselae subsp. damselae의 분리 및 특성을 보고하고자 한다.

Keywords

References

  1. Balebona MC, Andreu MJ, Bordas MA, Zorrilla I, Morinigo MA, Borrego JJ. Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.). Appl Environ Microbiol 1998; 64: 4269-4275.
  2. Bauer AW, Kirby WMM, Sherris JC, Truck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45: 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  3. Clinical and Laboratory Standards Institute. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria; Approved guideline M45-A. CLSI, Wayne, PA, USA 2006: 34-35.
  4. Fouz B, Barja JL, Amaro C, Rivas C, Toranzo AE. Toxicity of the extracellular products of Vibrio damsela isolated from diseased fish. Curr Microbiol 1993; 27: 341-347. https://doi.org/10.1007/BF01568958
  5. Fouz B, Toranzo AE, Marco-Noales E, Amaro C. Survival of fish-virulent strains of Photobacterium damselae subsp. damselae in seawater under starvation conditions. FEMS Microbiol Lett 1998; 168: 181-186. https://doi.org/10.1111/j.1574-6968.1998.tb13271.x
  6. Fouz B, Toranzo AE, Milan M, Amaro C. Evidence that water transmits the disease caused by the fish pathogen Photobacterium damselae subsp. damselae. J Appl Microbiol 2000; 88: 531-535. https://doi.org/10.1046/j.1365-2672.2000.00992.x
  7. Han JE, Gomez DK, Kim JH, Choresca Jr. CH, Shin SP, Baeck GW, Park SC. Isolation of Photobacterium damselae subsp. damselae from zebra shark Stegostoma fasciatum. Korean J Vet Res 2009; 49: 35-38.
  8. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Facultatively anaerobic gram-negative rods. In: Bergey's manual of determinative bacteriology, 9th ed. Baltimore: Williams and Wilkins Co. 1994: 177-204.
  9. 9. Hseu JR, Hwang PP, Ting YY. Morphometric model and laboratory analysis of intracohort cannibalism in giant grouper Epinephelus lanceolatus fry. Fish Sci 2004; 70: 482-486. https://doi.org/10.1111/j.1444-2906.2004.00829.x
  10. Morris JG JR, Wilson R, Hollis DG, Weaver RE, Miller HG, Tacket CO, Hickamn FW. Illness caused by Vibrio damsela and Vibrio hollisae. Lancet 1982; 319: 1294-1297. https://doi.org/10.1016/S0140-6736(82)92853-7
  11. Osorio CR, Romalde JL, Barja JL, Toranzo AE. Presence of phospholipase-D (dly) gene coding for damselysin production is not a pre-requisite for pathogenicity in Photobacterium damselae subsp. damselae. Microb Pathog 2000; 28: 119-126. https://doi.org/10.1006/mpat.1999.0330
  12. Osorio CR, Toranzo AE, Romalde JL, Barja JL. Multiplex PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae. Dis Aquat Org 2000; 40: 177-183. https://doi.org/10.3354/dao040177
  13. Pedersen K, Dalsgaard I, Larsen JL. Vibrio damsela associated with diseased fish in Denmark. Appl Environ Microbiol 1997; 63: 3711-3715.
  14. Takahashi H, Miya S, Kimura B, Yamane K, Arakawa Y, Fujii T. Difference of genotypic and phenotypic characteristics and pathogenicity potential of Photobacterium damselae subsp. damselae between clinical and environmental isolates from Japan. Microb Pathog 2008; 45: 150-158. https://doi.org/10.1016/j.micpath.2008.04.008