• Parasites, Hosts and Diseases
• /
• v.51 no.5
• /
• pp.599-602
• /
• 2013

A male patient with neurocysticercosis was identified in Montai Village, Xay District, Oudomxay Province, Lao PDR in February 2004. He had a history of diagnosis for neurocysticercosis by a CT scan in Thailand after an onset of epileptic seizure in 1993. A pig in the same district was found to contain Taenia solium metacestodes (=cysticerci); the slaughtered pig body contained more than 2,000 cysticerci. In addition to morphological identification, molecular identification was also performed on the cysticerci by DNA sequencing analysis of the mitochondrial cox1 gene; they were confirmed as T. solium metacestodes. The patient is regarded as an indigenous case of neurocysticercosis infected in an endemic focus of T. solium taeniasis/cysticercosis in Oudomxay Province, Lao PDR.

• Journal of Microbiology
• /
• v.43 no.2
• /
• pp.191-195
• /
• 2005

In this study, we attempted to characterize the Synechococcus sp. pee 7942 mutant resultant from a disruption in the gene encoding UDP-glucose: tetrahydrobiopterin a-glucosyltransferase (BGluT). 2D­PAGE followed by MALDI-TOF mass spectrometry revealed that phycocyanin rod linker protein 33K was one of the proteins expressed at lower level in the BGluT mutant. BGluT mutant cells were also determined to be more sensitive to high light stress. This is because photosynthetic O$_2$ exchange rates were significantly decreased, due to the reduced number of functional PSIs relative to the wild type cells. These results suggested that, in Synechococcus sp. pee 7942, BH4-glucoside might be involved in photosynthetic photoprotection.

• Journal of Environmental Impact Assessment
• /
• v.9 no.2
• /
• pp.119-126
• /
• 2000

Environmental monitoring system has been adopted and supplemented as inspection measures for the quantitative and qualitative changes of environmental impact assessment (EIA). Meanwhile it has been continuously pointed out that there is insufficient connection between environmental monitoring system and EIA. Often the agreed environmental impact assessment has not been fulfilled due to the argument of the cost, timing and situations of construction sites. Thus the purpose of this study is in search of the rationalized environmental monitoring system in order to harmonize the development and environmental conservation through the improvement of unreasonable aspects of the current EIA execution process. As to comply with the purpose, this research was carried out with three different but complimentary sources: environmental laws and regulations, foreign case studies of Japan, U.S.A. and Canada, and interviews with 73 experts. Finally, improved environmental monitoring system has been introduced reflecting the present process of EIA.

• Journal of the Korean Society of Earth Science Education
• /
• v.1 no.1
• /
• pp.99-110
• /
• 2008

The purpose of this study was to develop an instructional module for creativity(lMC) which was mainly based on a unit ('Sun's family' in the 2nd semester of the 5th grade in the textbook), to verify the effect of this module on Elementary student's creativity and attitudes related to science. The subject in this study was two 5th grade classes from J elementary school located in Busan. One of the group took the lessons which were designed for the IMC, and the other group took the normal classes. The effect of this subject for the experimental group and controlled group was verified by post-test: creativity and attitude related to science. The results of this study are as follows. First, the experimental group showed more creativity than controlled group on the post-test. The IMC was more effective to enhance student's creativity than general class. Second, the IMC was effective to enhance student's creativity without the difference in academic ability level. Third, the IMC was effective to enhance student's creativity without the difference in the gender. Fourth, the IMC was more effective to change student's attitude related to science than general class in the module application.

• Korean Journal Plant Pathology
• /
• v.13 no.4
• /
• pp.219-224
• /
• 1997

A PT-PCR (reverse transcription-polymerase chain reaction) diagnostic method for potato virus Y (PVY) was developed using primer pair derived from conserved region of coat protein genes of several PVY strains, A 764 bp PCR product was detected from several lines of potato cv. Atlantic. We could prove that the 764 bp DNA fragment was indeed the PVY gene by sequencing analysis. PVY detection method using RT-PCR technique was about tuber tissue.

• Journal of Applied Biological Chemistry
• /
• v.49 no.3
• /
• pp.75-81
• /
• 2006

A full-length cDNA encoding dihydroflavonol 4-reductase (st-dfr) of potato was isolated by rapid amplification of cDNA ends, and their expression was investigated from purple-fleshed potato (Solanum tuberosum L. cv. Jashim). The st-dfr exists as a member of a small gene family and its transcripts was abundant in the order of tuber flesh, stem, leaf, and root. The expressions of st-dfr gene were light inducible and cultivar dependant. Transgenic potato plants harboring antisense st-dfr (AS-DFR) sequences were analyzed. The accumulation of mRNA was nearly completely inhibited as a result of introducing an AS-DFR gene under the control of the 35S CaMV promoter into the red tuber skin Solanum tuberosum L. cv. Desiree. The anthocyanin content of the tuber peels of the transgenic lines was dramatically decreased by up to 70%. The possible production of flavonols in the peels of AS-DFR transgenic potatoes was discussed.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.6
• /
• pp.1604-1609
• /
• 2005

The purpose of this research was to investigate the effects of Bopaewon-tang (BT) on allergic reaction. In the present study, we examined the effect of BT on type 1 and type tV allergic reaction. BT (500 mg/kg) did not affect the systemic anaphylaxis induced by compound 48180 and the passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin in vivo. Also, BT did not affect the release of histamine from peritoneal mast cells in rats. In addition, BT did not affect the permeability of evans blue into peritoneal cavity, but inhibited the writhing syndrome induced by acetic acid. BT inhibited the delayed type hypersensitivity induced by SRBC and the contact dermatitis induced by dinitrofluorobenzene. These results indicate that BT may be useful for the prevention and treatment of type IV allergy related disease.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.3
• /
• pp.705-709
• /
• 2005

The purpose of this research was to investigate the effects of Coix Lachryma-Jobi L. var. Ma-yuen Stapf. on the proliferation of L1210 cells and immune cells. The hexane fraction of Coix Lachryma-Jobi decreased the proliferation of L1210 cells and induced DNA fragmentation of L1210 cells. Also, the fraction decreased the cell viability of murine thymocytes and splenocytes, but increased the phagocytic activity of murine peritoneal macrophages. In addition, fatty acids and fatty acid methyl esters decreased the proliferation of L1210 cells and induced DNA fragmentation of L1210 cells. The main components of the fraction are fatty acid and their derivatives. These results indicate that Coix Lachryma-Jobi has a cytotoxicity on tumor cells and immune cells.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.4
• /
• pp.960-964
• /
• 2005

We studied effects of Samreungjeon water extract (SE) on the proliferation of transplanted-L1210 cells to mice. Samreungjeon is composed of Scirpi Tuber, Zedoariae Rhizoma, Aurantii immaturi Pericarpium, Pinelliae Tuber and Hordei Fructus Germinatus. When SE (500 mg/kg) was administered orally once a day for 7 days after transplantation of L1210 cells to mice, the proliferation of transplanted-L1210 cells was decreased and DNA fragmentation of transplanted-L1210 cells was induced. Also, DNA fragmentation of L1210 cells was enhanced by co-culture with the peritoneal macrophages obtained from SE-administered mice and was partly inhibited by L-NMMA in vitro. SE enhanced the production of nitric oxide from murine peritoneal macrophages in vitro and in vivo. These results suggest that SE partly induces apoptosis of transplanted-L1210 cells via production of nitric oxide from macrophages.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2006.05a
• /
• pp.332-339
• /
• 2006

A leaf spring suspension has been widely used since it can carry big load and simplicity. But one major drawback is the poor ride performance because of the friction in the system and the high stiffness coefficient. To overcome these, air spring suspension can be used. The air spring suspension system can improve the ride of the heavy vehicle significantly and also it can adjust the height to the loading and unloading. A truck with the leaf suspension system is modified with the air suspension system and the performance of the vehicle is compared using the suggested method. The existing leaf suspension can be replaced with the air suspension system to improve the performance.