• YAKHAK HOEJI
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• v.52 no.2
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• pp.117-124
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• 2008

The leaves of Perilla frutescens Britt. var. japonica Hara (Labiatae) are often used in gourmet food in several Asian countries. Two kinds of perilla cultivars, Namcheon (NC) and Bora (BR), have been respectively developed in Korea by the pure line of 'deulkkae' from the local variety and by the cross of 'deulkkae' and 'chajogi'. The present study evaluated and compared antioxidant and neuroprotective effects of the fractions prepared from the leaves of the two cultivars using cell-free bioassay systems and primary cultured rat cortical cells. We found that the spirit, chloroform, hexane and butanol fractions from NC and BR leaves inhibited lipid peroxidation initiated in rat brain homogenates by $Fe^{2+}$ and L-ascorbic acid. In contrast, only the spirit and butanol fractions from both cultivars exhibited 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Among the fractions tested, the butanol fractions from NC and BR leaves exhibited the most potent antioxidant properties, and the butanol fraction from BR was more potent than the NC fraction. In consistence with these findings, the butanol fractions from both cultivars protected primary cultured cortical cells from the oxidative damage induced by $H_2O_2$ or xanthine and xanthine oxidase, with the BR butanol fraction being more active. The butanol fractions from NC and BR did not produce cytotoxicity in our cultures treated for 24 h at the concentrations of up to $100\;{\mu}g/ml$. Taken together, these results indicate that the leaves of the two cultivars of Perilla frutescens exert antioxidant and neuroprotective effects, and that the butanol fraction from BR leaves exhibits the most potent antioxidative neuroprotection among the fractions tested in this study.

• KSBB Journal
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• v.22 no.2
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• pp.78-83
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• 2007

In this study, we investigated the biological activity of antioxidant and antibacterial activity of Indigenous Plants, Jeju-Island., which, using methanol were extracted. The reducing activity on the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical and $O^{2-}$ and OH radical scavenging potential, in search for antioxidation activities of Indigenous Plants, were sequentially screened. Among the ten plant parts, Prunella vulgaris var. aleutica Fernald. flower had the highest antioxidative activity. 80% Methanol extracts of ten indigenous plants were screened for antibacterial activity 13 fish pathogenic bacteria by agar diffusion method. Among the various 80% Methanol extracts, the Prunella vulgaris var. aleutica Fernald, Gleichenia japonica Spreng, Microlepia marginata (panzer) Christ., Perilla frutescens var. japonica Hara. showed relatively strong antibacterial activities in the order.

• KSBB Journal
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• v.21 no.2
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• pp.164-169
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• 2006

In this study, we investigated the biological activity of antioxidant and antibacterial activity of Indigenous Plants, Jeju-Island., which, using water were extracted. The reducing activity on the 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical and $O^{2-}$ and OH radical scavenging potential, in search for antioxidation activities of Indigenous Plants, were sequentially screened. Among the ten plant parts, Prunella vulgaris var. aleutica Fernald. flower had the highest antioxidative activity. Hot water extracts of ten indigenous plants were screened for antibacterial activity 13 fish pathogenic bacteria by agar diffusion method. Among the various Hot water extracts, the Prunella vulgaris var. aleutica Fernald, Gleichenia japonica Spreng, Microlepia marginata(panzer) Christ., Perilla frutescens var. japonica Hara. showed relatively strong antibacterial activities in the order.

• Journal of Korean Society of Forest Science
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• v.84 no.4
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• pp.489-494
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• 1995

Seedlings and grafts of Betula pendula and Betula platyphylla var. japonica were grown under partially controlled environments in a greenhouse and in a plastic-greenhouse installed inside a laboratory. Plant growth conditions such as photoperiod, temperature, nutrient supply were partially controlled to enhance the vegetative and reproductive growth of the birch seedlings and grafts. By the treatments twenty and seventy one percents of the seedlings, respectively, for the Betula pendula and Betula platyphylla var. japonica developed visible floral organs between 250 to 508 days after seeding. By the same treatments eighty and fifty three percents of the grafts, respectively, for Betula pendula and Betula platyphylla var. japonica developed visible male catkins between 51 to 497 days and female catkins between 365 to 396 days after grafting. Breeding cycle of birch species can be reduced to a great extent by the induction of precocious flowering at early stages of seedling and graft development.

• Biomedical Science Letters
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• v.23 no.2
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• pp.64-72
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• 2017

The formation of brown colonies due to phenol oxidase activity on classic agar media containing natural material extracts of Helianthus annuus or on medium containing L-3,4-dihydroxyphenylalanine has been used to identify Cryptococcus species complex. In this study, various natural materials were used to develop a modified medium and to identify five major serotypes of Cryptococcus species complex. Serotypes A, D, and A/D were pigmented on medium using Perilla frutescens var. japonica Hara (PerJ agar) after a three-day incubation. Serotypes B and C were pigmented on PerJ agar after four- and five-day incubations, respectively. Growth time and pigmentation of the five serotypes occurred more rapidly on PerJ agar than on the other media. In addition, colony morphology, size, and pigmentation were specific by serotype. In conclusion, PerJ agar should be used in clinic settings to identify Cryptococcus species complex and its serotypes rapidly.

• Journal of the Korean Applied Science and Technology
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• v.34 no.2
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• pp.410-417
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• 2017

The physical properties of perilla leaves cultivated in Geumsan province were analyzed according storage conditions. The a/b values of perilla leaves increased with increasing storage period. Electronic nose composed of 12 different metal oxide sensors was used to differentiate flavors of perilla leaves. Sensitivities(delta $R_{gas}/R_{air}$) of sensors from electronic nose were obtained by principal compound analysis(PCA). Proportion of the first principal component was 93.07% at $25^{\circ}C$ and 97.81% at $4^{\circ}C$, respectively. In our result, flavor patterns of perilla leaves can be differentiated according to the storage temperature.

• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.19-22
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• 2008

This experiment was conducted at Sangji university in Wonju, Korea from 2005 to 2006. The results obtained were summarized as follow; The Chung-ju local variety was tested to find out the possibility of transplanting of seedling as a after-barley crop. When transplanting of seedling for a after-barley crop was test, this short-day plant flowered from september 1 to september 3, showing no connection with sowing time nor seedling periods. The yield of seeds per 10a was affected by both the sowing time and the seedling periods and its peak in the plots sowed on May 15 and transplanted on June 24(40 days seedling).

• Korean Journal of Medicinal Crop Science
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• v.12 no.2
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• pp.163-170
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• 2004

Secondary phenolic metabolites play an important role in plant defense mechanisms, and increasing evidence indicates that many phenolic compounds are important in human health. To date, few studies have investigated the impact of various medicinal plants on levels of secondary plant metabolites. To address this issue, 82 species of Korean medicinal plants were screened to determine their resveratrol contents. Among 82 medicinal plants, 5 species such as Gardenia jasmonoides, Phlomis umbrosa, Rheum palmatum L., Polygala tenuifolia, Rubus chingii HU contained relatively high concentrations of resveratrol $(179.75{\sim}42.71\;{\mu}g/g)$. But, 40 species including Adenophora triphylla var. japonica HARA were only observed low concentrations or trace of resveratrol, and 20 species including Alpinia officinarum HANCE did not contain a resveratrol.

• The Korean Journal of Community Living Science
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• v.26 no.4
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• pp.675-681
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• 2015

This study investigates traditional knowledge of plants used for river fishing in local communities of North Jeolla Province, Korea. Data were collected using the participatory rural appraisal method based on interviews, informal meetings, open and group discussions, and overt observations with semi-structured questionnaires. Field investigations were conducted at 19 sites from July 2008 to April 2010. Interviews included 37 key informants (16 men and 21 women) who lived more than 50 years in the area. The average age of the informants was 83 (range = 60 to 96). The analysis recorded seven species, namely Albizia julibrissin Durazz., Juglans mandshurica Maxim., Perilla frutescens var. japonica (Hassk.) H. Hara, Nelumbo nucifera Gaertn., Persicaria hydropiper (L.) Spach, Zanthoxylum piperitum (L.) DC., and Styrax japonicus Siebold and Zucc..

• The Journal of Natural Sciences
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• v.13 no.1
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• pp.83-96
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• 2003

Effect of Nutrient Concentrations, fertigation frequency, and learching percentage on crop growth and nutrient concentrations in root media were evaluated. The treatment of each irrigation with $50 mg.L^{-1}$ of nitrogen in stage 2 and increase to $80 mg.L^{-1}$ nitrogen in stage 3 had the highest crop growth at 34 days after sowing among treatments tested. Feeding with low nutrient concentrations and elevated frequency decreased crop growth. In treatments of each leaching percentage, feeding with low nutrient concentrations and elevated frequency resulted in increased tissue nutrient contents. The less tissue potassium content and higher calcium and magnesium contents were observed in treatment of 50% leach than those in 0% leach. All treatment tested had soil solution pH higher than 6.8. Electrical conductivity in treatments of 50% leach were lower than those of 0% leach. Feeding with low nutrient concentrations and elevated feeding frequency in each leaching percentage resulted in increased electrical conductivity in soil solution of root media. Trends of medium nutrient concentrations were similar to those of electrical conductivity.