• Title/Summary/Keyword: Ixeris dentata

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Effect on Antimutagenic and Cancer cell growth inhibition of Ixeris dentata Nakai (씀바귀의 항돌연변이성 및 암세포 성장억제효과)

  • Kim, Myong-Jo;Kim, Ju-Sung;Kang, Won-Hee;Jeong, Dong-Myong
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.2
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    • pp.139-143
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    • 2002
  • Ixeris dentate was used to extract the natural compounds with methanol and then the extracts were further fractionated using n-hexane, ethyl acetate, butanol and aqueous fraction. The methanol extract of Ixeris dentate had strong antimutagenic effect in Ames mutagenicity test. Among the extracts fractioned from the methanol extract, the butanol fraction exhibited the greatest antimutagenic effect suppressing the mutagenicity of Salmonella typhimurium TA100 with inhibition rate of 88.93%. Cancer cell lines include human lung carcinoma(A549), human breast adenocarcinoma(MCF-7) and human hepatocellular carcinoma(Hep3B). Hexane fraction showed the strongest effect against A549, MCF-7 and Hep3B at the same concentration compared to those of other fractions.

Polyphenol Analysis and Peroxynitrite Scavenging Effect of the Extracts from Eight Korean Mountainous Vegetable (한국 산채류 8종 추출물의 폴리페놀 함량분석 및 Peroxynitrite 소거효과)

  • Nugroho, Agung;Kim, Myung-Hoe;Lee, Jin-Ha;Kim, Jong-Dai;Lee, Kang-Ro;Choi, Jae-Sue;Yoo, Yeong-Min;Park, Hee-Juhn
    • Korean Journal of Pharmacognosy
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    • v.42 no.1
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    • pp.38-45
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    • 2011
  • Mountainous vegetables are usually used in the early stage of plant growth. We attempted to identify the quantity of polyphenols (caffoeoylquinic acids (CQs) and flavonoids) and peroxynitrite scavenging effect ($ONOO^-$) of eight Korean mountainous vegetables. The prominent characteristics were as follows: Only the roots of two plants Taraxacum platycarpum and Ixeris dentata contained chicoric acid. Five CQs were identified in the leaves of Cacalia firma whereas only 3-p-coumaroylquinic acid in the petioles of the plant was shown. The quantities of polyphenols such as quinic acid or tartaric acid derivatives in the mountainous vegetables were generally high, though those of flavonoids were very low. The $IC_{50}$ of chlorogenic acid, chicoric acid and kaempferol were 0.31, 0.12 and $0.25\;{\mu}g/ml$, respectively.

In Vitro Assessment on Biological Activities of Methanol Extracts from Several Compositae Edible Plants

  • Chon, Sang-Uk;Kim, Tae-Soon;Boo, Hee-Ock
    • Korean Journal of Plant Resources
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    • v.21 no.3
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    • pp.196-203
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    • 2008
  • Phytotoxicity, antioxidant activity, and cytotoxicity of the aqueous or methanol extracts from the young sprouts of the six Compositae medicinal plants were determined. Aqueous leachates at 40g dry tissue $L^{-1}$ (g $L^{-1}$) Cirsium japonicum and Aster yomena showed the highest inhibitory effect on alfalfa (Medicago sativa L.). Total phenolic content showed the highest amount in methanol extracts from Ixeris dentata, and followed by A. yomena, and Cephalonoplos segetum. Methanol extracts of C. segetum and I. dentata at 25${\mu}g$ m$L^{-1}$ exhibited the highest DPPH radical scavenging activity by 87.2, and 52.8%, respectively. By means of HPLC analysis, MeOH extracts of C. segetum had the highest amount of antioxidant chlorogenic acid. Based on MTT assay, the methanol extracts from Y. sonchifolia ($IC_{50}$ = 65.7${\mu}g$ $mL^{-1}$) showed the highest cytotoxicity against Calu-6. These results suggest that plant extracts had a dose-dependent biological potentials including phytotoxicity, antioxidant activity, and anticancer activity, and that their activities exhibited differently depending on plant species.

The Study on Native Plants and Planting Soil for Extensive Rooftop Greening (관리조방적 옥상녹화에 적합한 자생초화류와 식재토양에 관한 연구)

  • Kang, Kyu-Yi;Lee, Eun-Heui
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.8 no.4
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    • pp.23-31
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    • 2005
  • Green spaces in cities were insufficient and decreased as artificial structures increase. Accordingly, greening rooftop space contributed to urban ecosystems as green space. Especially, since 2002 Seoul supported rooftop greening and introduced 'Ecology-area rates'. Thus rooftop greening had the potentiality of extension. This prepared the extensive rooftop greenings within planting-base for extensive management rooftop greening system to be easily popularized. The extensive rooftop greening conducted from 1997-2005 reported that soil depth was 8cm and soil mixtures were 5 types. And plant were Crysanthemum zawaskii var. latilobum, Aster hayatae, Crysanthemum zawaskii herb. ssp. coreanum, Sedum sarmentosum, Sedum rotundifolium, Sedum oryzifolium, Sedum takemensis, Sedum middendorffianum, Sedum telerium var. purpureum, Sedum spectabile, Carypteris incana, Dianthus superbus, Hosta minor. As a result, for nine years after the modular type rooftop greening established, survival plants were Sedum takemensis, Sedum sarmentosum, Sedum spectabile, Sedum middendorffianum, Carypteris incana, Crysanthemum zawaskii var. latilobum, Sedum telerium var. purpureum, Sedum rotundifolium, Dianthus superbus. Also effect of greening was possibled soil depth 8cm. And growth of plants from 1997 to 2005 were fine on two soil mixtures of 'perlite+peatmoss+vermicompost+moisturizer' and 'perlite+moisturizer+bark(mulching)'. Invasive plants are Ixeris dentata Nakai, Ixeris chinensis var. strigosa, Youngia sonchifolia, Eragrostis ferruginea, Aster pilosus Willd., Ixeris japonica Nakai, Valpia myuros, and Setaria viridis. In conclusion, selection of suitable native plants was possible extensive management rooftop greening with effect of continuous greening. The extensive rooftop greening were lightweight and simple preparation without management and can popularize readily.

Screening of Biologically Active Compound from Edible Plant Sources-IX. Isolation and Identification of Sesquiterpene Lactons Isolated from the Root of Ixeris dentata forma albiflora; Inhibition Effects on ACAT, DGAT and FPTase Activity (식용식물자원으로부터 활성물질의 탐색-IX. 흰씀바귀(Ixeris dentata forma albiflora)뿌리에서 Sesquiterpene Lactone 화합물의 분리 및 구조 동정; ACAT, DGAT 및 FPTase 효소 활성의 저해)

  • Bang, Myun-Ho;Jang, Tae-O;Song, Myoung-Chong;Kim, Dong-Hyun;Kwon, Byoung-Mog;Kim, Young-Kuk;Lee, Hyun-Sun;Chung, In-Sik;Kim, Dae-Keun;Kim, Sung-Hoon;Park, Mi-Hyun;Baek, Nam-In
    • Applied Biological Chemistry
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    • v.47 no.2
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    • pp.251-257
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    • 2004
  • The root of lxeris dentata forma albiflora was extracted with 80% aqueous MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. From the EtOAc and n-BuOH fractions, four sesquiterpene compounds were isolated through the repeated silica gel and ODS column chromatographies. The chemical structures were determined as zaluzanin C (1), $9{\alpha}-hydroxyguaian-4(l5),10(14),11(13)-triene-6,12-olide$ (2), $3{\beta}-O-{\beta}-D-glucopyranosyl-8{\alpha}-hydroxyguaian-4(15),10(14 )-diene-6,12-olide$ (3), and $3{\beta}-O-{\beta}- D-glucopyranosyl-8{\beta}hydroxyguaian-10(14)-ene-6,12-olide$ (4) through the interpretation of several spectral data including 2D-NMR. Some showed the inhibitory effects on DGAT (Diacylglycerol acyltransferase), ($IC_{50}$ values of 1, 2: 0.13, 0.10 mM), the catalyzing enzymes of the intracellular esterification of diacylglycerol and FPTase (Famesyl-protein transferase), ($IC_{50}$ values of 1, 2: 0.15, 0.18 mM), the farnesylation enzyme for Ras protein charge of cancer promotion.

Effect of Aster scaber and Ixeris dentata on Contractility and Vasodilation of Cardiovascula and Endothelial Cell in Hyperlipidemic Rat (참취 및 씀바귀 첨가식이가 고지혈증 흰쥐의 심혈관 수축과 이완 및 혈관내피세포에 미치는 영향)

  • Lim, Sang-Sun;Lee, Jong-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.2
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    • pp.300-307
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    • 1997
  • The effects of Aster scaber and Ixeris dentata on cadiovascular system in hyperlipidemic rats were examined. Five groups of thirty Sprague Dawley rats were fed with the diet contained 1% cholesterol, 0.25% sodium cholate, 10% coconut oil and 5% lard(control group) for 4 weeks. Each experimental diet group was added with 5% plant powder or extract of the 5% plant powder by dry weight. Contractile or relaxation responses in the isolated artria and thoracic aortae were measured and the morphological changes of the aortic endotherium from the rats fed the experimental diet were inspected. In response to isoproterenol, the number of right atrial spontaneous beat was significantly lower in Cham chyi powder group$(PP_{1})$ and Sumbagui powder group$(PP_{2})$ than control at $10^(-8)M$ concentration. The contraction forces by injection of phenylephrine and calcium in isolated thoracic aorta were significantly low in each experimental groups compared with the control. The relaxation rates by acetylcholine represented comparatively higher value in $PP_{1}$ than control. The morphological changes of endothelial cell surface was a little in $PP_{1}$ and $PP_{2}$ compared with control, while the damages were considerably advanced in Cham chyi and Sumgbagui extract diet group$(PE_{1},\;PE_{2})$.

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Light Quality and Photoperiod Affect Growth of Sowthistle (Ixeris dentata Nakai) in a Closed-type Plant Production System (밀폐형 식물생산시스템에서 광질과 광주기에 따른 씀바귀의 생육)

  • Kim, Hye Min;Kang, Jeong Hwa;Jeong, Byoung Ryong;Hwang, Seung Jae
    • Horticultural Science & Technology
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    • v.34 no.1
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    • pp.67-76
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    • 2016
  • This study was conducted to examine the optimal environmental condition for promoting the growth of sowthistle as affected by light quality and photoperiod in a closed-type plant production system. Seeds were sown in 240-cell plug trays and then germinated for 3 days at a 24-hour photoperiod in a closed-type plant production system with LED lights (R:B:W = 8:1:1). Seedlings were transplanted and grown under 3 types of LED (R:B:W = 8:1:1, R:W = 3:7, or R:B = 8:2) and 4 photoperiods (24/0, 16/8, 8/16, or 4/20 hours) with $230{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ light intensity at a density of $20cm{\times}20 cm$. The experimental design was a randomized complete block design. Plants were cultured for 40 days un der the condition of $21{\pm}2^{\circ}C$ and $70{\pm}10%$ relative humidity after transplanting. Plants were fed with a recycling nutrient solution (pH 7.0 and EC $2.0dS{\cdot}m^{-1}$) contained in a deep floating tank. Fresh weight and dry weight of shoot or root, leaf length, and leaf area were the greatest in the photoperiod of 24/0 (light/dark) with RW LED. The highest number of leaves occurred in the photoperiod of 16/8 (light/dark) with RB LED, while the incidence of tip burn was higher in the photoperiod of 24/0 (light/dark) compared to the other treatments. Chlorophyll value was the highest in the 16/8 (light/dark) photoperiod and there was no significant difference by light quality. Chlorophyll fluorescence was the lowest in the photoperiod of 24/0 (light/dark) compared with other treatments. Therefore, in terms of economic feasibility and productivity for Ixeris dentata Nakai cultivation in a closed-type plant production system, the results obtained suggest that plants grew the best when kept in a photoperiod of 16/8 (light/dark) and light quality of combined LED RW (3:7).