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Molecular Identification of Gyrodinium impudicum and Gymnodinium sanguineum by Comparing the Sequences of the Internal Transcribed Spacers 1, 2 and 5.8S Ribosomal DNA

  • Kim Gi Young;Ha Myoung-Gyu;Cho Eun Seob;Lee Tae-Ho;Lee Sang Jun;Lee Jae-Dong
    • Fisheries and Aquatic Sciences
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    • v.2 no.1
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    • pp.66-77
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    • 1999
  • The sequences coding for the 5.8S rDNA and the internal transcribed spacers (ITS1 and ITS 2) from the isolates of nine isolates of Gyrodinium impudicum and two isolates of Gymnodinium sanguineum species were amplified, sequenced and compared with the previously known Alexandrium species and Gymnodinium catenatum. The genetic distance analyses based on the sequence alignment indicated that Gymnodinium catenatum and Gyrodinium impudicum species were some related, Alexandrium species was distant. G. catenatum and G. sanguineum were quite separate, but these two species belonged to the same genus. G. impudicum and G. catenatum forming the closet cluster showed some variation in the alignment of ITS regions. The length of ITS1 varied more than that of ITS2 and the length of ITS1 and ITS2 was different for each G. impudicum, Gymnodinium and Alexandrium species. Also, the length of ITS1 was shorter than that of ITS2. However, on the sequences of G. sanguineum, the length of ITS1 was longer about 23 nucleotides than that of ITS2. The phylogenetic analysis and rDNA similarity of G. impudicum and G. catenatum $(59\%)$ is higher than the that of G. catenatum and G. sanguineum $(55\%)$. It was thought that the phylogenetic analysis and the genetic distance revealed that G. impudicum and G. catenatum were clearly different species and G. impudicum may belong to the genus of Gymnodinium.

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A Multi Medium Wireless Communication Vehicle Terminal Development and a Performance Test (다매체 무선통신 차량 단말 개발 및 성능평가)

  • Kim, Sang-Heon;Cho, Yung-Sung;Kim, Kyoung-Hwan;Bae, Myoung-Hwan;Lee, Kyeong-Im
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.10 no.5
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    • pp.135-145
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    • 2011
  • As communication technology, an enabling technology for ITS, becomes diversified with DSRC, CDMA, Wireless LAN, it is expected that type and function of ITS services will become gradually expanded. As a result, research and development of a standard platform that can provide support so that numerous communication technologies that are being used in various ITS services to receive various ITS services can be operated in one integrated terminal are being carried out. On Board Unit (OBU) for a recent standard platform was also built and tested recently. However, verification for standard suitability of the standard platform OBU that was developed must be carried out. Standard technology must be realized and sufficient functional and operational testing must be carried out in order to guarantee efficient operation in an actual ITS system and increase utilization of standard technology. Therefore, In this paper we had constructed test road and we had tested a standard platform OBU for a standard application.

A Design of Advanced Traveler Information System based on Component (컴포넌트에 기반한 여행자정보고급화 시스템의 설계)

  • Kim, Jin-Hwan;Chang, Jea-Young;Lee, Bong-Gyou
    • Journal of Korea Spatial Information System Society
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    • v.3 no.1 s.5
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    • pp.37-48
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    • 2001
  • ITS includes an Advanced Traveler Information System(ATIS) that provides travelers with service and facility data for the purpose of assisting prior to embarking on a trip or after the traveler is underway. ATIS consists of three major subsystems, which are a Pre-trip Traffic Information subsystem(PTIS), an En-route Traffic Information Subsystem(ETIS), and a Dynamic Route Guide Subsystem(DGIS). ATIS needs to be designed and implemented in accordance with the National ITS Architecture, a reference framework that spans all of standards activities. Recently, as software technology is rapidly improved and stabilized, there are some needs to reuse pre-developed and powerful ITS technology. ITS standardization based on components and open interfaces becomes a way to solve these reusability of current ITS technology. This paper focuses on how could we design and implement ATIS based on the component with the aid of UML(Unified Modeling Language). The UML methodology is expected to provide a standardized model for newly developed ITS components.

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우리나라 S/W 벤처기업의 경영현황

  • 한계섭;손성호
    • Proceedings of the Korea Association of Information Systems Conference
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    • 2000.11a
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    • pp.26-31
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    • 2000
  • It is said that the focus of managing venture business is currently moving from technology competition to management competition. By the way, the software venture business(SVB) has some weak points in its structural composition and itematization and no professional personnel in other several sections except technology development section. In addition, such basic functions as technology and R & D, finance and accounting, marketing required to the management of business are concentrated on only one man, its representative director. Therefore, this study aims to provide the basic data useful to the establishment of governmental policy in information and communication, to the rearing of the SVB by a local government related to the software, and to the administration of SVB by investigating the actual conditions. This study attempts to examine the literature on venture business and software industry, and its management with a questionnaire about the actual conditions of managing the SVB. The questionnaire is given to 527 local enterprises belonging to the Software Industry Association and to 171 enterprises in the Software Center. This study compromises the characteristics of the SVB, the actual conditions of its technology and R & D, finance and accounting, and marketing. The characteristics of the SVB are classified into categories such as the stage of its growth(the stage of its seed and start-up, the stage of tis development and growth, the stage of its stability and maturity) and the main business(the system integration, the software development for contract, the package software development service, the software-related service). Additionally, the study attempts to analyze positively the actual condition of its management after classified by the areas of business profile, its general management, its technology development, its finance and accounting, and its marketing The result of this study is found that the SVB has a lot of troubles in part of marketing and finance & accounting activity as well as general management. The SVB realizes the importance of the technology development rather than that of management activity including marketing activity. So we expect this study can assist the SVB to establish the business guidelines for own management plans.

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Study on the Conformance Testing of Data Exchange between Transport Information Center and Terminal Equipment (교통정보센터와 단말기간 데이터교환 기술기준 적합성 시험에 관한 연구)

  • Lee, Sang-Hyun;Kim, Gyeong-Seok
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.7 no.5
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    • pp.147-158
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    • 2008
  • Recently, Intelligent Transportation System (ITS) has been actively developed and built since the Transportation System Efficiency Promotion Act was enacted. However, since mutual connection among transportation information systems was not considered, the integration of transportation information services did not occur. Accordingly, the Ministry of Land Transport and Maritime Affairs established and announced the technical standard on ITS. In this study, the conformance testing of the transportation information and communication system interface standard on data exchange between the Transportation Information Center and terminals was researched The test items were categorized as data request tests and data providing tests by analyzing the communication procedures specified in the standard. A detail testing scenario was created for each item. The test assessment was established based on the conformance of data exchange procedures and the accuracy of data packet messages. Under the established technical standard, the number of times that tests should be performed was thought set to 30 and the success rate was set to 95%. The purpose of this study is to help the ITS of Korea perform the integrated management of transportation information by researching methods for conformance testing on the technical standard on ITS.

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A Study on the Effectiveness of Intelligent Transport Systems on National Highways (국도상의 지능형교통시스템의 효과성 분석에 관한 연구)

  • Lim, Sung-Han;Kim, Hyun-Suk;Heo, Tae-Young
    • Journal of Korea Society of Industrial Information Systems
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    • v.14 no.4
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    • pp.205-212
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    • 2009
  • Intelligent Transport Systems(ITS) has been currently growing attention in industry as hightech traffic system and ITS infrastructure has been built not only on expressway but also on national highways. Although the effect of ITS installation on national highways is not easy to measure with quantitative methodology, it is necessary to develop the quantitative method to verify the effect accurate analysis of ITS effect. In this study, the analysis of cost efficiency of ITS project carried out by Iksan Regional Constriction Management Administration(IRCMA) was conducted. Analysis period and discount rate were assumed as 10 years and 5.5%, respectively. Several measures of the effect including reduction of travel time, CO2 discharged and fuel and the value of Variable Message Sign(VMS) information were proposed. Concludingly, ITS project implemented by IRCMA appeared to be cost effective, indicating 1.20 of B/C ratio, 12.4% of IRR and W1.48 billion of NPV.

Phylogenetic Analysis of the Genus Phellinus by Comparing the Sequences of Internal Transcribed Spacers and 5.8S Ribosomal DNA (Ribosomal DNA의 Internal Transcribed Spacer(ITS) 부위의 염기서열분석에 의한 Phellinus속의 계통분석에 관한 연구)

  • Chung, Ji-Won;Kim, Gi-Young;Ha, Myung-Gui;Lee, Tae-Ho;Lee, Jae-Dong
    • The Korean Journal of Mycology
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    • v.27 no.2 s.89
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    • pp.124-131
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    • 1999
  • This study was carried out to identify the phylogenetic relationship among Phellinus species by comparing the DNA sequences of the 5.8S ribosomal DNA (rDNA) and the internal transcribed spacers (ITSs), ITS1 and ITS2 regions. Two primers from the 3' end of 18S rDNA and the 5' end of 28S rDNA sequences were chosen to amplify the specific ITS regions of Phellinus spp. Phellinus strains used in the study were divided into four clusters by the phylogenetic tree based on the amplified regions of ITS and 5.8S rDNA sequences. The first cluster consist of Phellinus hartigii IMSNU 32041 and Phellinus robustus IMSNU 32068, and the second cluster consists of Phellinus linteus strains and Phellinus weirianus IMSNU 32021. Phellinus laevigatus KCTC 6229, KCTC 6230 and Phellinus igniarius KCTC 6227, KCTC 6228 belong to the third cluster. Finally, Phellinus chrysoloma KCTC 6225 and Phellinus chrysoloma KCTC 6226 are the fourth cluster. In the second cluster the differentiation between Phellinus linteus strains and Phellinus weirianus species were not possible by the comparison of the ITS sequences. These results revealed that Phellinus linteus and Phellinus weirianus cannot be established the concept of species level only by the ITS sequences. Therefore, both physiological and molecular biological methods as well as the sequences of type strains are necessary to classify the strains of these two species accurately. The comparison of the ITS sequences of four Phellinus species indicated that the sequences of the ITS1 generally are more divergent than those of the ITS2. Although the ITS sequences are varied in some species, the conserved regions in both ITS1 and ITS2 are useful tool to differentiate the species. Phellinus linteus and related species have their specific sequences in the ITS1 compared to the other species.

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