• Title/Summary/Keyword: Isolation resistance

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Prevalence of Staphylococcus hyicus subsp hyicus in pigs with reference to antibiotic susceptibility of isolates (돼지에 있어서 Staphylococcus hyicus subsp hyicus의 분포와 분리균의 항균제 감수성)

  • Lee, Dong-weon;Yeo, Sang-geon
    • Korean Journal of Veterinary Research
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    • v.30 no.1
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    • pp.51-57
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    • 1990
  • In order to know the prevalence of S. hyicus subsp hyicus in pigs, attempts were made to isolate the organism from 549 healthy adult pigs, 277 healthy suckling piglets and 17 piglets with exudative epidermitis from April 1988 to January 1989 in Chinju, Korea. Also determined was antibiotic susceptibility of the isolates. Isolation rates of S. hyicus subsp. hyicus from adult pigs and suekling piglets were 27.0% and 53.1%, respectively. The organism was isolated predominantly from abdomen (10.9%) of adult pigs and external ear (31.6%) of suckling piglets. Isolation rates of the organism from piglets of different age group were in order of prevalence of 1(82.1%), 3(74.0%), 2(54.7%), 4(52.9%), 5(15.2%) and 6(5.7%) post-natal weeks. All of 489 isolates were sensitive to gentamicin, kanamycin and cephalexin but 23.7~79.6% of the strains were resistant to erythromycin, penicillin G and tetracycline. The 49.2% of these resistant strains exhibited multiple drug resistance. The drug resistant patterns most frequently encountered were PG TC EM(10.7%) in triple pattern, PG TC(23.0%) in double pattern and TC(44.4%) in single pattern.

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Broadband Microwave SPDT Switch Using CPW Impedance Transform Network (CPW 임피던스 변환회로를 이용한 광대역 마이크로파 SPDT 스위치)

  • Lee Kang Ho;Park Hyung Moo;Rhee Jin Koo;Koo Kyung Heon
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.42 no.7 s.337
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    • pp.57-62
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    • 2005
  • This paper describes the design of a high performance microwave single pole double throw (SPDT) monolithic microwave integrated circuit switch using GaAs pHEMT process. The switch design proposes a novel coplanar waveguide (CPW) impedance transform network which results in the low insertion loss and high isolation by compensating for the FET parasitics to get the low on-resistance and low off-capacitance. The proposed switch has the measured isolation of better than 24 dB and insertion loss of less than 2.6 dB from 53 to 61 GHz. The chip is fabricated with the size of 2.2mm $\times$ 1.6 mm.

A Study on Testing of 1/4-scale and Full-size Seismic Isolation Bearings (축소모델과 실모델 면진베어링의 성능실험에 관한 연구)

  • 정민기;정지만;김두훈
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 1996.04a
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    • pp.194-202
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    • 1996
  • An approach to increase the seismic resistance of large structures is to reduce the seismic forces, to which structures are subjected by base isolation systems. The anti-seismic performance of base-isolated beatings has been verified experimentally by shaking table tests. However, it may be difficult to perform the tests for the full-scale beatings of base-isolated structures. Therefore, the test program was designed to evaluate the reliability and properties of the beatings under a range of loading conditions including axial stress, loading frequency and direction, and temperature. The effects of scale were also evaluated by comparing the results of the 1/4-scale beatings with those from the full-scale bearings, and the ultimate behavior of both types of bearings with evaluated through a series of roll-out tests. This report draws comparisons among the different tests and bearings to determine the importance of various factors including load history, axial stress, and frequency. Comparisons between the 1/4-scale bearings were difficult because of the scaling effects in manufacturing and thermal radiation, but qualitative results from the 1/4-scale bearings can certainly be extrapolated the full-scale bearings.

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Comparative study of the seismic response of RC framed buildings retrofitted using modern techniques

  • Mazza, Fabio
    • Earthquakes and Structures
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    • v.9 no.1
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    • pp.29-48
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    • 2015
  • The main purpose of this work is to compare different criteria for the seismic strengthening of RC framed buildings in order to find the optimal combinations of these retrofitting techniques. To this end, a numerical investigation is carried out with reference to the town hall of Spilinga (Italy), an RC framed structure with an L-shaped plan built at the beginning of the 1960s. Five structures are considered, derived from the first by incorporating: carbon fibre reinforced polymer (FRP)-wrapping of all columns; base-isolation, with high-damping-laminated-rubber bearings (HDLRBs); added damping, with hysteretic damped braces (HYDBs); FRP-wrapping of the first storey columns combined with base-isolation or added damping. A three-dimensional fibre model of the primary and retrofitted structures is considered; bilinear and trilinear laws idealize, respectively, the behaviour of the HYDB, providing that the buckling be prevented, and the FRP-wrapping, without resistance in compression, while the response of the HDLRB is simulated by using a viscoelastic linear model. The effectiveness of the retrofitting solutions is tested with nonlinear dynamic analyses based on biaxial accelerograms, whose response spectra match those in the Italian seismic code.

Study on the Evaluation Method of Electrical Isolation Property for Hydrogen Fuel Cell Vehicle in Post Crash (수소연료전지자동차의 충돌시 절연성능 평가방법에 관한 고찰)

  • Lee, Kiyeon;Gil, Hyoungjun;Kim, Dongook;Kim, Dongwoo;Kang, Daechul
    • Transactions of the Korean hydrogen and new energy society
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    • v.23 no.6
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    • pp.612-618
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    • 2012
  • In this paper, in order to prevent electric shock of high voltage system of HFCV after crash test, insulation performance measurement methods were studied. Under conditions of in-use, insulation performance tests can be divided into measurement method using the vehicle's own RESS as DC voltage source and measurement method using DC voltage from off-vehicle sources. However, these tests can not be applied after a post-crash because parts of high voltage system cover should be removed, and insulation performance can be influenced during these tests. Therefore, we proposed post-crash insulation performance test methods for preventing electric shock through problem analysis of previous post-crash insulation performance tests. Also, test equipment which can measure voltage absence and total energy was developed. We verified performance of the equipment through experiments with vehicle test.

Flowable oxide CVD Process for Shallow Trench Isolation in Silicon Semiconductor

  • Chung, Sung-Woong;Ahn, Sang-Tae;Sohn, Hyun-Chul;Lee, Sang-Don
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.4 no.1
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    • pp.45-51
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    • 2004
  • We have proposed a new shallow trench isolation (STI) process using flowable oxide (F-oxide) chemical vapor deposition (CVD) for DRAM application and it was successfully developed. The combination of F-oxide CVD and HDP CVD is thought to be the superior STI gap-filling process for next generation DRAM fabrication because F-oxide not only improves STI gap-filling capability, but also the reduced local stress by F-oxide in narrow trenches leads to decrease in junction leakage and gate induced drain leakage (GIDL) current. Finally, this process increased data retention time of DRAM compared to HDP STI. However, a serious failure occurred by symphonizing its structural dependency of deposited thickness with poor resistance against HF chemicals. It could be suppressed by reducing the flow time during F-oxide deposition. It was investigated collectively in terms of device yield. In conclusion, the combination of F-oxide and HDP oxide is the very promising technology for STI gap filling process of sub-100nm DRAM technology.

Isolation of Citrate-Utilizing Variants of Escherichia coli from Cattle, Pigs, Chickens, Pigeons and Wild Animals (동물유래(動物由來)의 Citrate이용대장균(利用大腸菌) 변이주(變異株)에 관하여)

  • Lee, Hun-Jun;Choi, Won-Pil
    • Korean Journal of Veterinary Research
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    • v.23 no.2
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    • pp.173-178
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    • 1983
  • This paper deals with the isolation of citrate-utilizing variants of Escherichia coli ($Cit^+$ E. coli) from the animals, their biochemical reactivity and antibiotic susceptibility, and whether the citrate utilizing ability is transmissible. One hundred arid twenty-three isolates of $Cit^+$ E. coli were obtained from cattle and pigs. but from other animals, no isolates were obtaied. Antibiotic susceptibility testing of $Cit^+$ E. coli was performed by the agar dilution method, using the following 9 antibiotics, chloramphenicol(CP), tetracycline(TC), streptomycine(SM), kanamycin(KM), colistin(CL), gentamicin(GM), cephaloridine(CR), aminobenzycillin and nalidixic acid(NA). All the variants tested were susceptible to KM, CL, GM, CR and NA. Of all the variants, 80(65%) were resistant to the drugs tested and resistance to TC and SM was most frequent. The transmission of the ability to utilize citrate on Simmons citrate agar at $37^{\circ}C$ was demonstrated in 78(67.8%) out of the 115 $Cit^+$ E. coli. There were no significant difference in the transfer rates of citrate utilizing ability between resistant and susceptible variants to above 9 drugs. Of 123 isolates, 8 were lost their citrate utilizing ability, spontaneously.

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Vertical distributions of lateral forces on base isolated structures considering higher mode effects

  • Tsai, C.S.;Chen, Wen-Shin;Chen, Bo-Jen;Pong, Wen-Shen
    • Structural Engineering and Mechanics
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    • v.23 no.5
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    • pp.543-562
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    • 2006
  • Base isolation technology has been accepted as a feasible and attractive way in improving seismic resistance of structures. The seismic design of new seismically isolated structures is mainly governed by the Uniform Building Code (UBC-97) published by the International Conference of Building Officials. In the UBC code, the distribution formula of the inertial (or lateral) forces leads to an inverted triangular shape in the vertical direction. It has been found to be too conservative for most isolated structures through experimental, computational and real earthquake examinations. In this paper, four simple and reasonable design formulae, based on the first mode of the base-isolated structures, for the lateral force distribution on isolated structures have been validated by a multiple-bay three-story base-isolated steel structure tested on the shaking table. Moreover, to obtain more accurate results for base-isolated structures in which higher mode contributions are more likely expected during earthquakes, another four inertial force distribution formulae are also proposed to include higher mode effects. Besides the experimental verification through shaking table tests, the vertical distributions of peak accelerations computed by the proposed design formulae are in good agreement with the recorded floor accelerations of the USC University Hospital during the Northridge earthquake.

Isolation and Identifition of DAP-Auxotrophs from E. coli pRDI (E. coli pRDI에서의 DAP-영양요구성 변이주 분리 및 동정)

  • 이호자
    • Korean Journal of Microbiology
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    • v.22 no.4
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    • pp.265-269
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    • 1984
  • For the utilization as donor cells of conjugation, DAP-Auxotrophs were isolated from C. coli cells, carrying plasmid $p^{RD1}$ with(a) drug resistance makers from Pseudononas $(Km^r, \;Carb^r, \;Tc^r)$ and (b) the nif-gene group from Klebsiela. E. coli $p^{RD1}$ cells were treated with nitrosoguanidine for the mutagenesis and cephalexin for the isolation of DAP-Auxotrophs. The nature of auxotrophs was verified by suitable biochemical test and checking with 6-cyanopurine as a color indicator for the presence of nif-gene.

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Current Methods of Circulating Tumor Cell Detection (순환종양세포 검출 기술)

  • Lim, Minji;Cho, Yoon-Kyoung
    • The Korean journal of helicobacter and upper gastrointestinal research
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    • v.18 no.3
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    • pp.157-161
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    • 2018
  • Liquid biopsy, the analysis of circulating biomarkers from peripheral blood, such as circulating tumor cells (CTCs) and circulating tumor DNA, and exosomes, offers a less invasive, new source of cancer-derived materials that may reflect the status of the disease better and thereby contribute to personalized treatment. Recent advances in microfluidics and molecular analysis technologies have resulted in greatly improved CTC enumeration and detection. In this article, we review commercially available technologies used to isolate CTCs from peripheral blood, including immunoaffinity and label-free, physical property-based isolation methods. Although enormous technological progress has been made, especially within the last decade, only a few CTC detection methods have been approved for routine clinical use. Here, we provide an overview of the current CTC isolation methods and examples of their potential application for early diagnosis, prognosis, treatment monitoring, and prediction of resistance to cancer therapy. Furthermore, the challenges that remain to be addressed before such tools are implemented for routine use in clinical settings are discussed.