• 대한의생명과학회지
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• 제18권2호
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• pp.96-103
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• 2012

Kv 4.2 ion channel protein has an ability to open at subthreshold membrane potentials and to recover quickly from inactivation. That is very important for neuronal signal transmission in vertebrate brain. In order to purify Kv 4.2 protein, the novel purification methods were experimented. The purification procedure utilized chromatography on DE-52 ion exchange column and affinity chromatography on a WGA-Sepharose 4B, and Kv 4.2 affinity column chromatography. It was found that 0.5% (wt./vol.) Triton X-100 detergent in lysis buffer worked well for Kv 4.2 protein solubilization from rat brain membrane. Protein quantitative determination was conducted by BCA method at 562 nm for each purification step to avoid determination interference of protein at 280 nm by detergent. The confirmation of Kv 4.2 existence and amount is performed using by SDS-PAGE/immunoblotting or 96-well dot blotting. The Kv 4.2 without interacting protein that contains carbohydrate, was purified from novel biochemical 3-steps purification method for further research.

• The Korean Journal of Physiology and Pharmacology
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• 제20권1호
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• pp.119-127
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• 2016

Dihydropyridine (DHP) calcium channel blockers (CCBs) have been widely used to treat of several cardiovascular diseases. An excessive shortening of action potential duration (APD) due to the reduction of $Ca^{2+}$ channel current ($I_{Ca}$) might increase the risk of arrhythmia. In this study we investigated the electrophysiological effects of nicardipine (NIC), isradipine (ISR), and amlodipine (AML) on the cardiac APD in rabbit Purkinje fibers, voltage-gated $K^+$ channel currents ($I_{Kr}$, $I_{Ks}$) and voltage-gated $Na^+$ channel current ($I_{Na}$). The concentration-dependent inhibition of $Ca^{2+}$ channel currents ($I_{Ca}$) was examined in rat cardiomyocytes; these CCBs have similar potency on $I_{Ca}$ channel blocking with $IC_{50}$ (the half-maximum inhibiting concentration) values of 0.142, 0.229, and 0.227 nM on NIC, ISR, and AML, respectively. However, ISR shortened both $APD_{50}$ and $APD_{90}$ already at $1{\mu}M$ whereas NIC and AML shortened $APD_{50}$ but not $APD_{90}$ up to $30{\mu}M$. According to ion channel studies, NIC and AML concentration-dependently inhibited $I_{Kr}$ and $I_{Ks}$ while ISR had only partial inhibitory effects (<50% at $30{\mu}M$). Inhibition of $I_{Na}$ was similarly observed in the three CCBs. Since the $I_{Kr}$ and $I_{Ks}$ mainly contribute to cardiac repolarization, their inhibition by NIC and AML could compensate for the AP shortening effects due to the block of $I_{Ca}$.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2010년도 추계학술대회 논문집
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• pp.439-440
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• 2010

• Bulletin of the Korean Chemical Society
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• 제20권10호
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• pp.1181-1185
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• 1999

The reaction of FeC5H5+ ion with ferrocene molecule is investigated using FT-ICR mass spectrometry. FeC5H5+ ions are generated by dissociative ionization of ferrocenes using an electron beam. The reaction gives rise to the formation of the adduct ion, Fe2(C5H5)3+, in competition with charge transfer reaction leading to the formation of ferrocene molecular ion, Fe(C5H5)2+·. The branching ratio of the adduct ion increases as the internal energy of the reactant ion decreases and correspondingly the branching ratio for the charge transfer reaction product decreases. The observed rate of the addition reaction channel is slower than that of the charge transfer reaction. The observation of the stable adduct ions in the low-pressure ICR cell is attributed to the radiative cooling of the activated ion-molecule complex. The mechanism of the reaction is presented to account for the observed experimental results.

• Progress in Superconductivity
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• 제14권2호
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• pp.82-86
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• 2012

A sensitive eight-channel high-Tc Superconducting Interference Device (SQUID) detection system for magnetic contaminant in a lithium ion battery anode was developed. Finding ultra-small metallic foreign matter is an important issue for a manufacturer because metallic contaminants carry the risk of an internal short. When contamination occurs, the manufacturer of the product suffers a great loss from recalling the tainted product. Metallic particles with outer dimensions smaller than 100 microns cannot be detected using a conventional X-ray imaging system. Therefore, a highly sensitive detection system for small foreign matter is required. We have already developed a detection system based on a single-channel SQUID gradiometer and horizontal magnetization. For practical use, the detection width of the system should be increased to at least 65 mm by employing multiple sensors. In this paper, we present an 8-ch high-Tc SQUID roll-to-roll system for inspecting a lithium-ion battery anode with a width of 65 mm. A special microscopic type of a cryostat was developed upon which eight SQUID gradiometers were mounted. As a result, small iron particles of 35 microns on a real lithium-ion battery anode with a width of 70 mm were successfully detected. This system is practical for the detection of contaminants in a lithium ion battery anode sheet.

• 대한전자공학회논문지
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• 제21권4호
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• pp.58-64
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• 1984

이온주입형 E-IGFET에서 이온주입층내 불순물 profile을 임의의 형태로 가정하였으며, 가정한 불순물 profile을 이용하여 threshold 전압에 대한 간단한 model을 유도하였다. 유도한 model을 이용하여 Gaus-sian-profile일 때의 threshold 전압치를 구하였고, 실제의 측정 data와 비교하였을 때 일치함을 확인할 수 있었다. 더불어, box-profile일 때의 threshold 전압치의 오차를 계산해 보았다. 또한, substrate-bias에 의한 threshold 전압의 변화를 simulation하였으며. 계산과정에서 이온주입층의 깊이 D를 구하는 새로운 방법을 제시하였다.

• Journal of Ginseng Research
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• 제32권2호
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• pp.99-106
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• 2008

A ligand - whether an endogenous hormone, neurotransmitter, exogenous toxin or synthetic drug - binds to plasma membrane proteins (e.g., ion channels, receptors or other functional proteins) to exert its physiological or pharmacological effects. Ligands can also have functional groups, showing stereospecificity for interaction sites on their counterpart plasma membrane proteins. Previous reports have shown that the ginsenoside Rg$_3$, a bioactive ginsenoside, meets these criteria in that: 1) an aliphatic side chain of $Rg_3$ plays a role as a functional group, 2) Rg$_3$ regulates voltage- and ligand-gated ion channels in a stereospecific manner with respect to carbon-20, and 3) $Rg_3$ regulates subsets of ligand-gated and voltage-gated ion channels through specific interactions with identified amino acid residues inside the channel pore, in the outer pore entryway, or in toxin binding sites. Rg$_3$, therefore, could be a candidate for a novel ginseng-derived glycosidic ligand regulating ion channels and receptors. This review will examine how Rg$_3$ regulates voltage-gated and ligand-gated ion channels through interactions with its target proteins in the plasma membrane. Hopefully, this review will advance understanding of ginseng pharmacology at the cellular and molecular levels.

• The Korean Journal of Physiology and Pharmacology
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• 제7권5호
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• pp.267-273
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• 2003

We have investigated the effect on inducing substate(s) of positively charged residues replaced in position 172 of the second transmembrane domain in murine inward rectifier potassium channels, formed by stable or transient transfection of Kir2.1 gene in MEL or CHO cells. Single channel recordings were obtained from either cell-attached patches or inside-out patches excised into solution containing 10 mM EDTA to rule out the effect of $Mg^{2+}$ on the channel gating. The substate(s) could be recorded with all mutants D172H, D172K and D172R. The unitary current-voltage (I-V) relation was not linear with D172H at $pH_i$ 6.3, whereas the unitary I-V relation was linear at $pH_i$ 8.0. The relative occupancy at $S_{LC}$ was increased from 0.018 at $pH_i$ 8.0 to 0.45 at $pH_i$ 5.5. In H-N dimer, that was increased from 0.016 at $pH_i$ 8.0 to 0.23 at $pH_i$ 5.5. The larger the size of the side chain or $pK_a$ with mutants (D172H, D172K and D172R), the more frequent the transitions between the fully open state and substate within an opening. The conductance of the substate also depended upon the pKa or the size of the side chain. The relative occupancy at substate $S_{LC}$ with monomer D172K (0.50) was less than that in K-H dimer (0.83). However, the relative occupancy at substate with D172R (0.79) was similar to that with R-N dimer (0.82). In the contrary to ROMK1, positive charge as well as negative charge in position 172 can induce the substate rather than block the pore in murine Kir2.1. The single channel properties of the mutant, that is, unitary I-V relation, the voltage dependence of the mean open time and relative occupancy of the substates and the increased latency to the first opening, explain the intrinsic gating observed in whole cell recordings.

• 한국농공학회논문집
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• 제56권4호
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• pp.21-28
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• 2014

In this study, evaluated ware the strength and durability of the vegetated water purification channel concrete to which recycled aggregates, hawang-toh and jute were applied. Box-Behnken method of response surface analysis in statistics was applied to the experimental design. Experimental variables are as follows, recycled coarse aggregates, hawang-toh, blast-furnace slag and jute fiber. In the experiment, conducted were the tests of compressive strength, chloride ion penetration, abrasion resistance and impact resistance the replacement rate effects of the recycled aggregates, blast-furnace slag and hwang-toh on the performance of vegetated water purification channel concrete were analyzed by using the response surface analysis method on the basis of the experimental results. In addition, an optimum mixing ratio of vegetated water purification channel concrete was determined by using the experimental results. The optimum mixing ratio was determined to be in 10.0% recycled coarse aggregates, 60.0% blast-furnace slag, 10.1% hwang-toh and 0.16% jute fiber. The compressive strength, chloride ion penetration, abrasion rate, and impact number of fracture test results of the optimum mixing ratio were 24.1 MPa, 999 coulombs, 10.30 g/mm3, and 20 number, respectively.

• 대한전자공학회논문지
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• 제21권5호
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• pp.67-78
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• 1984

프로팅 게이트 위에 컨트롤 게이트를 갖는 n-채널 이중 다결정 실리콘게이트 EAROM을 제작하였다. 채널 길이는 4-8μm, 채널 폭은 5-14μm로 하여 5μm design rule에 따라 설계하였으며 서로 다른 4가지 컨트롤게이트 구조를 갖는 채널 주입형 기억소자를 얻었다. 그리고 소자의 Punch through 전압과 게이트에 의해 조절되는 채널파괴 전압을 증가시키기 위해 이중 이온주입 (double ion implantation)과 active 영역에 보론이온을 주입 하였다. 프로그래밍을 위해 드레인 전압 및 게이트 전압이 각각 13-l7V 및 20-25V 정도 필요하였다. 그리고 제조된 기억소자의 소거는 광학적 방법뿐 아니라 전기적 방법으로도 가능하였으며 125℃에서 200시간 유지하였을 때 축적된 전자가 약 4 %정도 감소함을 알 수 있었다.