• BMB Reports
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• 제38권2호
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• pp.128-150
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• 2005

Invertebrate animals, which lack adaptive immune systems, have developed other systems of biological host defense, so called innate immunity, that respond to common antigens on the cell surfaces of potential pathogens. During the past two decades, the molecular structures and functions of various defense components that participated in innate immune systems have been established in Arthropoda, such as, insects, the horseshoe crab, freshwater crayfish, and the protochordata ascidian. These defense molecules include phenoloxidases, clotting factors, complement factors, lectins, protease inhibitors, antimicrobial peptides, Toll receptors, and other humoral factors found mainly in hemolymph plasma and hemocytes. These components, which together compose the innate immune system, defend invertebrate from invading bacterial, fungal, and viral pathogens. This review describes the present status of our knowledge concerning such defensive molecules in invertebrates.

• 생명과학회지
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• 제28권3호
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• pp.300-306
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• 2018

라이소자임은 선천성 면역 물질로 개불을 포함하는 여러 무척추동물의 병원균에 대한 방어에 주요하게 작용한다. 본 논문은 개불(Urechis unicinctus)의 체벽 조직 추출물로부터 무척추형 라이소자임의 정제와 그 특성에 관한 분석을 기술하고 있다. 체벽 추출물은 우선적으로 Sep-Pak C18 cartridge를 사용하여 부분적으로 분리되었으며, 분리된 분획 중 60% 메탄올에 용출된 분획이 Bacillus subtilis KCTC 1021에서 강한 항균활성을 나타내었다. 그 후 여러 단계의 역상과 이온교환 고속액체크로마토그래피(High Performance Liquid Chromatography, HPLC)를 사용하여 항균성 물질이 정제되었으며, 분자량은 약 14 kDa이었다. 이 단백질의 일차서열은 LC-MS/MS를 통해 분석되었으며, 얻은 부분적 아미노산 서열을 NCBI BLAST를 통하여 분석해 본 결과, 이 항균성 물질은 다른 동물들로부터 동정된 무척추동물형 라이소자임(invertebrate-type lysozyme)의 서열과 유사도를 가지고 있어, 체벽으로부터 정제된 개불 라이소자임(Urechis unicinctus invertebrate-type lysozyme from body wall, Uu-iLysb)으로 명명하였다. 개불 라이소자임의 활성을 확인하기 위하여 항균활성 및 라이소자임 효소 활성실험을 진행한 결과, 개불 라이소자임이 항균활성과 라이소자임 효소활성 모두 강하게 가지고 있는 것을 확인하였다.

• 한국토양동물학회지
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• 제2권2호
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• pp.83-91
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• 1997

One year study with litter bags(mesh size - 0.4mm, 0.8mm, 1.7mm and 5.0mm) was carried out to investigate the soil invertebrate community in the process of leaf decomposition in Namsan and Kwangreung deciduous forests, which were considered to be under different degrees of environmental selective pressure. Soil animals collected from litter bags were classified into the class of order or higher taxa. Acari and Collembola were major groups: Acari and Collembola were about 60% and 30% of total soil animals in their numbers, respectively. Among minor groups, Dipteria, Araneae, Diplopoda, Coleoptera and Chilopoda were comparatively dominant. In Namsan forest which was considered to be under higher environmental selective pressure than Kwangreung, the densities of Acari and Collembola were somewhat higher than in Kwangreung, although there was no statistically significant difference between two sites. The densities of Chilopoda, Enchytraeidae and Nematoda were much higher in Namsan than in Kwangreung but Diplopoda and Symphyla were much more in Kwangreung. It was expected that those groups could be used as bioindicators. The densities of Acari and Collembola were very low until March and then showed the peak in May. But they decreased slowly until November. There was no significant difference among the mesh sized of litter bags in the densities of Acari and Collembola but other groups of soil invertebrates seemed to be prevented from immigrating into the litter bag of mesh size 0.4mm. Decomposition rate of litter in the litter bag was low in early stage of decomposition. The % residual mass over initial mass at 8 months after litter bag introduction in the field was over 80%. Thereafter, % residual mass decreased more fast and was about 60% at 1 year after bag introduction. There was little evidence for the effects of soil invertebrates upon the litter decomposition in the period of this study. And there was no significant difference between Namsan and Kwangreung or among mesh sizes of litter bags in the decomposition rate.

• Animal cells and systems
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• 제16권5호
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• pp.351-356
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• 2012

Food is important to any animal, and a large part of the behavioral repertoire is concerned with ensuring adequate nutrition. Two main nutritional sensations, hunger and satiety, produce opposite behaviors. Hungry animals seek food, increase exploratory behavior and continue feeding once they encounter food. Satiated animals decrease exploratory behavior, take rest, and stop feeding. The signals of hunger or satiety and their effects on physiology and behavior will depend not only on the animal's current nutritional status, but also on its experience and the environment in which the animal evolved. In our novel, nutritionally rich environment, improper control of appetite contributes to diseases from anorexia to the current epidemic of obesity. Despite extraordinary recent advances, genetic contribution to appetite control is still poorly understood partly due to lack of simple genetic model systems. In this review, we will discuss current understanding of molecular and cellular mechanisms by which animals regulate food intake depending on their nutritional status. Then, focusing on relatively less known muscarinic and cGMP signals, we will discuss how the molecular and behavioral aspects of hunger and satiety are conserved in a simple invertebrate model system, Caenorhabditis elegans so as for us to use it to understand the genetics of appetite control.

• 한국유기농업학회지
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• 제25권4호
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• pp.875-901
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• 2017

유기농업이 생물 다양성에 미치는 효과가 제한적이라는 일부 회의적인 시각이 있긴 하지만, 유기농업은 토양의 물리적 및 생태학적 특성과 생물 다양성에 영향을 미치는 것으로 받아들여지고 있다. 본 연구는 충청북도 괴산지역에서 유기농업과 관행농업을 시행하는 논과 밭을 대상으로 경작 기간 중 채집된 무척추동물의 종을 분류 동정하여 농법의 차이에 따른 생물 다양성의 변화를 비교 분석하였다. 조사 기간인 2016년 6월부터 10월까지 채집된 총 249종의 무척추동물 중에서, 유기농업을 시행하는 지역의 논과 밭에서는 각각 104종과 108종이 관찰된 반면, 관행농업을 시행하는 논과 밭에서는 각각 84종과 77종이 관찰되어, 산술적으로 관행농업 지역에 비해 유기농업 지역의 종 다양성이 약 20% 높은 것으로 비교되었다. 특히 유기농업의 시행에 따라 곤충류와 거미류의 분류군에서 긍정적인 종 다양성 변화가 관찰되었다. 한편, 유기농업 논에서는 노린재목, 파리목, 벌목, 거미목 등의 분류군이, 그리고 유기농업 밭에서는 노린재목, 딱정벌레목, 거미목 등이 관행농업 지역의 논과 밭에 비해 생물 다양성이 증가하는 것으로 분석되어, 유기농업 시행에 따른 생물 지표종의 선정을 위해서는 향후 이들 분류군에 대한 정밀한 조사가 필요할 것으로 생각된다.

• 한국자기공명학회논문지
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• 제24권4호
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• pp.104-116
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• 2020

Fipronil, the phenylpyrazole insecticide, is effective and used in various fields. Especially, fipronil was reliable because it was known to be specific on invertebrate animals than vertebrate animals including mammals. However, fipronil had potential risks that affect vertebrate animals as it blocks the gamma-aminobutyric acid (GABA) receptors that also exists in vertebrates as well as invertebrates. Therefore, it was necessary that harmful effects of fipronil on vertebrates are clarified. For this purpose, the zebrafish (Danio rerio) were used on behalf of vertebrate animals in present study. The zebrafish were exposed to 5 ㎍/L, 25 ㎍/L, and 50 ㎍/L of fipronil during 12, 24 and 72 hours. To closely observe toxic process, 12 hours and 24 hours of additional time point were set in the exposure test. Nuclear magnetic resonance (NMR)-based metabolomics is an approach to detect metabolic changes in organism resulted from external stimuli. In this study, NMR-based metabolomics showed the metabolic changes in zebrafish caused by fipronil exposure. Metabolic analysis revealed that fipronil interfered with energy metabolism and decreased the antioxidant ability in zebrafish. Antioxidant ability decline was remarkable at high exposure concentration. In addition, metabolic analysis results over time suggested that reactions for alleviating the excessive nerve excitation occurred in zebrafish after fipronil exposure. Through this study, it was elucidated that the adverse effects of fipronil on vertebrate animals are evident. The risk of fipronil on vertebrates can be no longer ignored. Moreover, this study has a meaning of practically necessary research for organism by examining the effects of fipronil at low concentrations existed in real environment.

• 한국수산과학회지
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• 제38권2호
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• pp.100-105
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• 2005

Two species of fish and five species of marine invertebrate showed different tolerances to the toxicity of composite slag and bituminaus coal. Especially, Hemicentrotus pulcherrimus and young Haliotis discus hannai displayed marked differences in tolerance from. H. pulcherrimus and young H. discus hannai showed lethal effects at higher concentrations than those concentrations of the composite slag in the 1.0 and $0.4\%$ range, respectively. H. pulcherrimus showed no lethal effects at a lower concentration of $1.0\%$ composite slag and some differences in the rate of oxygen consumption with this concentration of composite slag. The lethal effects of bituminous coal on marine and fisheries organisms, even with higher concentrations, were not observed. At a higher concentration than that of 500 mg/L (ppm) of bituminous coal, decrease effects appeared in the rate of oxygen consumption of the experimental organisms. Taking into consideration that the experimental concentration of composite slag and bituminous coal were impracticable in the ocean, the results of this experiment suggest that composite slag and bituminous coal pose no real threat to marine or fisheries organisms.

• 한국환경교육학회지:환경교육
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• 제11권1호
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• pp.275-290
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• 1998

Nowadays, mankind is suffering from serious pollutions. In proportion to industrial modernization, polltional problems have been increased and arised new one endlessly. Todays, we are cope with a newkind pollution materials threatening the life of mankind with root out. It is Environmental Hormon, that is, Endocrine Disrupting Chemical(EDs). EDs was made by dioxin, PCB, DDT, cadmium, lead, ether and even diethylstillbestrol(DES) that used to contraceptive pill etc. These EDs makes changing from normal human hormon to abnormal one and it kill off or fade out spermatozoa. Then the spermatozoa was infected with EDs makes man imposex, sexual malformations and infertility also. It is the more critical phenomenons that EDs invade into the field of wild-life; birds, invertebrate animals, fishes, mammalia especially. England TV(BBC) journalist Cadury, D. introduced a lot of case studies that many kind of male animals was changed feminization by EDs in her book (The Feminization of Nature;1997). Otherwise, Gibbs, P.E. and Bryan, G. W. reported about dog-whelk snail (a sort of sea shell) was changed gender from male to female by EDs in their study. Briefly speaking, Environmental Hormon (EDs) stem from polluted materials maloperate human and animal informational codes of hormon. It is a significant event to an individual ontogeny and ecological system. To overcome these problem WHO, OECD, UNEP held many work-shops, international seminars and researches but it is not enough yet. On May of 1997, the International Meeting of Environmental Administrators makes a $\ulcorner$Declaration on EDs Problems$\lrcorner$ for solving it fortunately and NHK(japan) reported Super Critical Fluid as a new materials for decrease toxicity of dioxin in recent. Key Words : Environmental Hormon, EDs, imposex feminization, gender, DES, spermatozoa. Super Critical Fluid.

• 한국지구과학회지
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• 제39권1호
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• pp.23-45
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• 2018

Moderately diverse, but very abundant trace fossils are found from the Late Pleistocene deposits of Jeju Island, Korea. Vertical I-, Y- and U-shaped domichnia of annelids or decapods are, over 2500, extremely abundant, 3D network domichnia of callianassids are, over 200, very abundant, and small sinuous trails of nematode repichnia are, over 50, abundant in number. Horizontal trails attributable to polychaete or worm-like animals are, less than 50, common, but horizontal spreiten burrows, fish traces and crab trackways are, less than 10, rare in occurrence. Of these trace fossils, Taenidium barretti, Undichna britannica and Undichna unisulca represent the first record from the Pleistocene in Asia. Psilonichnus upsilon is the second record in Asia. Crab trackways probably produced by underwater punting gait of sideway walking crabs may represent the first record in the world. In addition, diverse and very abundant footprints of more than 500 hominids, more than 200 birds and more than 1000 mammals are closely associated with these invertebrate trace fossils. Trace fossil assemblage integrated with sedimentary facies is interpreted to have been formed in the marginal marine foreshore to backshore environment corresponding to the Psilonichnus and Skolithos ichnofacies.

• Ocean and Polar Research
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• 제24권4호
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• pp.457-464
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• 2002

Distribution and stock assessment of mega-benthos living in the shallow hard bottom around Dokdo were studied in July,2000. Depth and topographical conditions have affected to the densities and biomass of benthic animals. In shallow area, less than 10 m depth, turbo shell Batillus cornutus, mussel, Mytilus corusucs were dominated and showed distinct patterns in vertical distribution. On the other hand, the area over 10m depth, it showed diverse pattern depending on topography. Turbo shell, mussel and sea cucumber Stichopus japonicus were dominated in terms of fishery resources, but abalones were rarely sampled. Stock assessment were estimated to be 6.54 M/T, 3.89 M/T and 8.92 M/T, respectively. Some parts of coastal hard bottom around Dokdo, such as the area between Dongdo and Seodo, seemed to play an Important role as nursery ground. Therefore, it is necessary to the environmental monitoring for coastal fishery managements aspects.