• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.10a
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• pp.916-919
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• 2002

Cam mechanism is one of the common devices used in lots of automatic machinery. This paper introduces to an inverse cam mechanism. The inverse cam mechanism has a reverse structure as compared with common cam mechanism. For shape design of the inverse cam the approach used in this paper is an instant velocity center method that find the contact point between cam and roller at any contact time. And a computer program is developed for shape design and simulation by visual $C^{++}$ language. As the results, this paper presents two examples for the shape design of the inverse cam mechanism in order to prove the accuracy of the design procedures.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.1299-1302
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• 2005

Current mechanical devices are trending toward being a small size, high speedy, automation. For performing these functions, machinery elements organizing a machine should be designed exactly. Cams have high confidence and economics in ablility to transmit a motion. Accordingly, A cam mechanism is very important for processing the machine automatically. This paper introduce an inverse cam mechanism. A square shaped cam which cannot be commonly analyzed is designed and manufactured by using the NURBS interpolation algorithm. The objective of this paper is to develop a computer-aided design program. In this paper, a displacement curve of oscillating motion inverse cam mechanism with square shaped follower is analyzed. The data is redistibuted by the NURBS algorithm. A cam shape is designed by the instant velocity center method, and simulated to verify the validity of the operation state.

• 한국기계연구소 소보
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• s.17
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• pp.83-91
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• 1987

This paper is concerned on kinematic Analysis and Simulation of an automatic feeding mechanism and a curved inverse cam. The main objective is the development of an algorithm of the cam-feeding mechanism and a CAD program. The computer program CACAFS (Computer-Aided Cam and Automatic Feeding System is developed by using the state-of-the-art for CAD. Thus, the program CACAFS is independent of computer hardwares and also interactive. As the first part of paper, this paper introduces the algorithm to analyze the motion of the inverse cam and the automatic feeding mechanism. The second part of paper presents the technique for the mechanism simulation and graphics-oriented CAD programming.

• 제어로봇시스템학회:학술대회논문집
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• 1987.10b
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• pp.264-268
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• 1987

This paper is concerned on kinematic analysis and simulation of an automatic feeding mechanism subjected by the motion of a curvilinear inverse can. The curvilinear cam is rotated by positioning a translating roller and the automatic feeding mechanism is moved to the sliding position by the motion of a campin fixed on the curvilinear cam. The curvilinear cam consists of two arcs of circles and two straight lines. The modular approach is used for the kinematic analysis of the feeding mechanism. As the first part of the paper for the motion simulation of the cam-feeding system, this paper discusses the algorithm to simulate the motion of the cam-feeding mechanism. The second part of the paper presents the state-of-art for the graphics-oriented CAD technique,

• 한국기계연구소 소보
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• s.17
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• pp.93-97
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• 1987

This paper is concerned on kinematic analysis and simulation of an automatic feeding mechanism and a curved inverse earn. The main objective is the development 0 fan algorithm of the earn-feeding mechanism and a CAD program. The computer program CACAFS (Computer-Aided Cam and Automatic Feeding System) is developed by using the state-of-the-art for CAD. Thus, the program CACAFS is independent of computer hardwares and also interactive. As the second part of paper, this paper introduces the technique for the mechanism simulation and graphics-oriented CAD programming. The first part of r paper presents the algorithm to analyze the motion of the inverse earn and the automatic feeding mechanism.

• 제어로봇시스템학회:학술대회논문집
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• 1987.10b
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• pp.269-272
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• 1987

This paper is concerned on kinematic analysis and simulation of an automatic feeding mechanism subjected by the motion of a curvilinear inverse cam. The main objection is the development of computer-aided design (CAD) program for simulating the motion of the cam-feeding mechanism using computer-graphics. A computer program CACAFS (Computer-Aided Cam and Automatic Feeding System) is independent of computer hardware used. The program is also interactive using a menu-selection technique. As the second part of the paper for the motion simulation of the cam-feeding system, this paper discusses the state-of-art for CAD. The first part of the paper presents the algorithm to simulate the notion of the cam-feeding mechanism.

• Journal of Life Science
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• v.22 no.7
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• pp.863-870
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• 2012

Rhodopsin, a dim light photoreceptor, has been regarded as one of the model systems for the structural and functional study of G protein-coupled receptors (GPCRs). Constitutively active mutant GPCRs leading to the activation of heterotrimeric GDP/GTP-binding protein signaling in the absence of ligand binding are of interest for the study of the activation mechanism in GPCRs. The present study focused on the opsin mutant E134Q/M257Y, which showed a moderate level of constitutive activity and the formation of two distinct rhodopsin chromophores with absorption maxima of 500 nm and 380 nm, depending on the presence of an inverse agonist, 11-cis-retinal, and an agonist, all-trans-retinal, respectively. Reconstitution of the mutant rhodopsin upon incubation with different ratios of 11-cis-retinal and the all-trans-retinal, as well as upon sequential binding of the two retinals, indicated its preferential binding to 11-cis-retinal. The thermal stability of the 11-cis-retinal-bound form of the E134Q/M257Y mutant was lower than that of the mutants containing a single replacement but higher than that of the all-trans-retinal-bound forms. The mutant also showed a lower stability in its opsin state as compared with that of the wild-type opsin but had little effects on the binding affinity to 11-cis-retinal. Information obtained in this study will be helpful for analyzing the structural changes associated with the activation of rhodopsin and GPCRs.

• Journal of Life Science
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• v.17 no.6 s.86
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• pp.783-790
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• 2007

G protein coupled receptors (GPCRs) transmit various extracellular signals into the cells. Upon binding of the ligands, conformational changes in the extracellular and/or transmembrane (TM) domains of CPCRs were propagated into the cytoplasmic (CP) domain of the molecule leading to the activation of their cognate heterotrimeric C proteins and kinases. Constitutively active GPCR mutants causing the activation of C Protein signaling even in the absence of ligand binding are of interest for the study of activation mechanism of GPCRs. Two classes of constitutively active mutations, categorized by their effects on the salt bridge between Ell3 and K296, were found in the TM domain of rhodopsin. Opsin mutants containing combinations of the mutations were constructed to study the conformational changes required for the activation of rhodopsin. Rhodopsin chromophore regenerated with 11-cis-retinal showed a thermal stability inversely correlated with its constitutive activity. In contrast, rhodopsin mutants exhibited a binding affinity to an agonist, all-trans-retinal, in a constitutive activity-dependent manner. In order to test whether the conformational changes responsible for the activation of trans-ducin (Gt) are the same as the conformation required for the recognition of rhodopsin kinase, analysis of the mutants were carried out with phosphorylation by rhodopsin kinase. Rhodopsin mutants containing combinations of different classes of the mutations showed a strong synergistic effect on the phosphorylation of the mutants in the dark as similar to that of Gt activation. The results suggest that at least two or three kinds of segmental and independent conformational changes are required for the activation of rhodopsin and the conformational changes responsible for activating rhodopsin kinase and Gt are similar to each other.