• 제목/요약/키워드: Intra-Variability

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Analysis of Visual Sensibility Evaluation of Naturally Colored Organic Cotton: Identification of Reliability and Proper Scouring Method

  • Park, Jang-Woon;Chang, Yoon;Hong, Won-Gi;Lee, Myung-Eun;Han, Ah-Reum;Chae, Young-Joo;Cho, Gil-Soo;You, Hee-Cheon
    • 대한인간공학회지
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    • 제30권2호
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    • pp.311-317
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    • 2011
  • Objective: The present study was intended to identify (1) the intra- and inter-rater reliabilities of a visual sensibility evaluation protocol and (2) the effects of NaCOC color and scouring method on the visual sensibility of NaCOC. Thirty female participants(20s & 30s) were participated in the visual sensibility evaluation of NaCOC. Background: Interests in naturally colored organic cotton(NaCOC) increase rapidly in parallel with the social trend of eco-friendly living and wellbeing. Method: Three color sets (ivory, green, and coyote-brown) of NaCOC specimens including one untreated and four treated specimens($Na_2CO_3$; NaOH; enzyme; boiling water) were examined in the study. The visual sensibility evaluation was conducted by the test-retest method using nine pairs of bipolar visual sensibility adjectives(bright-dark; clear-murky; heavy-light; vivid-subdued; warm-cool; fresh-stale; strong-weak; showy-plain; and luxurious-cheap). Results: As a result of reliability of a visual sensibility evaluation protocol, inter-rater variability(average SD=1.06) of visual sensibility evaluation was more than 1.4 times the intra-rater variability(average SD=0.74). However, both the sensibility evaluation reliabilities did not show any systematic pattern of changes. Lastly, ANOVA and post-hoc analysis showed that preferred scouring methods for a visual sensibility adjective pair significantly vary depending on NaCOC color. Application: Both the reliability of visual sensibility evaluation protocol and the analysis of proper scoring method of NaCOC in the study would be useful information to design the affective textile.

A Critical Evaluation of DNA Adducts as Biological Markers for Human Exposure to Polycyclic Aromatic Compounds

  • Godschalk, Roger W.L.;Van Schooten, Frederik-Jan;Bartsch, Helmut
    • BMB Reports
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    • 제36권1호
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    • pp.1-11
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    • 2003
  • The causative role of polycyclic aromatic hydrocarbons (PAH) in human carcinogenesis is undisputed. Measurements of PAH-DNA adduct levels in easily accessible white blood cells therefore represent useful early endpoints in exposure intervention of chemoprevention studies. The successful applicability of DNA adducts as early endpoints depends on several criteria:i.adduct levels in easily accessible surrogate tissues should reflect adduct levels in target-tissues, ii. toxicokinetics and the temporal relevance should be properly defined.iii. sources of inter- and intra-individual variability must be known and controllable, and finally iv. adduct analyses must have advantages as compared to other markers of PAH-exposure. In general, higher DNA adduct levels or a higher proportion of subjects with detectable DNA adduct levels were found in exposed individuals as compared with non-exposed subjects, but saturation may occur at high exposures. Furthermore, DNA adduct levels varied according to changes in exposure, for example smoking cessation resulted in lower DNA adduct levels and adduct levels paralleled seasonal variations of air-pollution. Intra-individual variation during continuous exposure was low over a short period of time (weeks), but varied significantly when longer time periods (months) were investigated. Inter-individual variation is currently only partly explained by genetic polymorphisms in genes involved in PAH-metabolism and deserves further investigation. DNA adduct measurement may have three advantages over traditional exposure assessment: i. they can smooth the extreme variability in exposure which is typical for environmental toxicants and may integrate exposure over a longer period of time. Therefore, DNA adduct assessment may reduce the monitoring effort. ii. Biological monitoring of DNA adducts accounts for all exposure routes. iii. DNA adducts may account for inter-individual differences in uptake, elimination, distribution, metabolism and repair amongst exposed individuals. In conclusion, there is now a sufficiently large scientific basis to justify the application of DNA adduct measurement as biomarkers in exposure assessment and intervention studies. Their use in risk-assessment, however, requires further investigation.

Development of Computerized Densitometry for the Quantitative Analysis of Diffuse Retinal Nerve Fiber Layer Atrophy

  • 이재성;박광석;이가영;김동명
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1997년도 추계학술대회
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    • pp.146-149
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    • 1997
  • Computerized densitometry was developed or the quantitative measurement of diffuse retinal nerve fiber layer (RNFL) atrophy and intra- and inter-operator reliability and clinical validity of this system were evaluated. Vertical diameter, center of the optic disc, and peripapillary circles which had radii of 1.5 and 2.5 times that of the optic disc were user-interactively determined in digitized RNFL photograph and density profile along each circle was measured and normalized. The areas under the normalized density profiles of the superior and the inferior segments in both circle were used or the study of RNFL. To determine the variability and correspondence in the measurements of density variations, 21 RNFL photographs of glaucoma patients which showed varying degrees of atrophy underwent computerized densitometry by two operators on two separate occasions. Coefficient of variation in the densitometric measurements was $1.2{\sim}5.4%$. Intra- and inter-operator reliabilities were excellent. The correlations between the densitometric values and mean deviations of Humphrey C30-2 visual field showed statistical significance. Computerized densitometry of RNFL photographs was useful in the objective and quantitative assessment of diffuse RNFL atrophy.

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비빈곤가정과 빈곤가정 유아의 문제행동 발달궤적과 학습준비도 및 학교적응 (Children's Problem Behaviors Trajectories of Poor- and Non Poor-Households on the Path to Learning Readiness and School Adjustment)

  • 이완정;김미나
    • Human Ecology Research
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    • 제56권2호
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    • pp.157-165
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    • 2018
  • Using data from the Panel Study on Korean Children, this study investigated whether children with high levels of problem behaviors adjusted more poorly on the $1^{st}-grade$ than children with low levels of problem behaviors, and whether there was evidence of intra-individual stability in behavior problems over time. Data were analyzed by use of the Latent Growth Model and group differences analyses. Three findings were noteworthy. First, there was evidence of intra-individual and inter-individual variability in behavior problems between poor- and non-poor household children. Second, children with higher initial levels of internalizing and externalizing behaviors at 4 years had lower school readiness scores at 6 years. Finally, children with lower levels of school readiness at 6 years had lower school adjustment scores in $1^{st}$ grade. The results discuss implications for future research and policies for preschool children. With mediating effect of school readiness, developmental trajectories of child's problem behavior have been found to be predictors of delayed achievements in school. The results show that intervention programs are necessary for children with high levels of problem behavior. This study also showed that children who experienced poverty at home could have more difficulties in school readiness and school adjustment.

파노라마방사선사진과 다면상재구성 CT상의 치조골 높이 계측에 대한 비교 연구 (Comparative study on alveolar bone height of pantomography and multi planar reformatted computed tomography)

  • 지정현;이상래;이병도
    • Imaging Science in Dentistry
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    • 제34권3호
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    • pp.159-164
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    • 2004
  • Purpose: To compare alveolar bony height of pantomograph with bony height of thin slice, multiplanar reformatted (MPR) Computed Tomograph. Materials and Methods : Panoramic radiograms of 12 young adult patients had been taken by one radologic technitian and the measurements were corrected by magnification ratio (1.20). The slice thickness of Multi-detector Computed Tomography (CT) was at least 1mm for the accuracy. The raw CT datas were imported into the V-works 4.0 (CyberMed Corp., Seoul, Korea) and transformed to MPR images. Pantomographic measurements of alveolar bone were compared to CT values by average mean bony height measurements for the accuracy. Inter-, and Intra-observer variability was evaluated. Results : There was no significant differences between height measurement of pantomography and that of CT (P>0.05). There were no significant differences in either inter-or intra-observer measurements (P>0.05). Conclusion : Pantomography showed relatively high accuracy and precision in measuring alveolar bony height.

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수입 축산식품에서의 잔류농약 동시분석법 개발에 관한 연구 (Study on the development of simultaneous-rapid screening methods for residues pesticides in livestock products)

  • 김종수;김곤섭;하대식
    • 대한수의학회지
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    • 제37권1호
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    • pp.213-220
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    • 1997
  • A simple and rapid method known as matrix solid phase dispersion(MSPD) for simultaneous determination of 11 pesticide(2,4,5,6-tetrachlor m-xylene, ${\alpha}$-BHC, ${\gamma}$-BHC, ${\delta}$-BHC, aldrin, chlorfulazuron, heptachloroepoxide, dieldrine, endrin, endosulfan sulfate, and tetradifon) in beef fat was estabilished. Beef fat(0.5g) was fortified by adding the 11 pesticides and dibutylchlorendate as internal standard, and blended with 2g bulk $C_{18}$ in pestle and motar. Pesticides were eluted from an extraction column composed of $C_{18}$/ fat matrix blend and 2g activated Florisil by addition of 8ml acetonitrile. Then $2{\mu}l$ portion of the acetonitrile elute was directly analyzed by gaschromatography with electron capture detection. Unfortified blank control were treated similarly. Recovery rate were ranged from $83{\pm}5.4%$ to $94.2{\pm}7.6%$, intra-assay variability and inter-assay variability were ranged from 2.3% to 7.4%(n=5 for each insecticides) and from $6{\pm}1%$ to $12{\pm}3%$(n=10 for each insecticides), respectively. These results indicated that the MSPD methodology is aceptable for the extraction, determination and screening of residues 11 chroniated pesticides in beef fat.

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Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP

  • Kamran, Muhammad;Javed, Nazir;Ullah, Ihsan;Nazir, Shahid;Jamil, Shakra;Iqbal, Muhammad Zafar;Abbas, Huma;Khan, Sajid Aleem;Haq, Muhammad Ehetisham ul
    • The Plant Pathology Journal
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    • 제35권1호
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    • pp.51-62
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    • 2019
  • A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures ($25{\pm}2^{\circ}C$ and $30{\pm}2^{\circ}C$) and concentrations ($10^4$ and $10^5$ spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.

Variability of Surface Water Properties in the Japan/East Sea on Different Time Scales

  • Ponomarev, Vladimir;Rudykh, Natalya;Dmitrieva, Elena;Ishida, Hajime
    • Ocean and Polar Research
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    • 제31권2호
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    • pp.177-187
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    • 2009
  • This study examined the multi-scale variabilities of sea surface temperature (SST) and salinity in the Japan/East Sea (JES) based on statistical analyses of observational data, with a focus on the northwestern part of the sea. The regionality of JES SST variability was estimated for different frequency ranges on semimonthly (11-17 days), monthly to seasonal (30-90 days), quasi-semiannual (157-220 days), and quasi-biennial (1.5-3 years) time scales using cluster analyses of daily gridded SST data for 1996 to 2007 from the Japan Meteorological Agency (JMA). Several significant peaks and regional cores were found in each frequency range of the SST anomaly (SSTA) oscillations. Quasi-semiannual SSTA oscillations with high amplitude were found in the south-southwestern part of the Japan Basin ($41-43^{\circ}N$) and were amplified in the area adjacent to Peter the Great Bay. Oscillations with periods of 79 and 55 days also prevailed over the southwest Japan Basin between the Yamato Rise and the continental slope. A similar method was applied to classify SST and the annual cycle of surface salinity using Generalized Digital Environmental Model (GDEM) gridded data. The Tatarskii Strait and adjacent area showed the most specific annual cycles and variability in salinity on interannual to interdecadal time scales. The most significant inverse relationship between surface salinity in the Tatarskii Strait and southern JES areas was found on the interdecadal time scale. Linkages of sea water salinity in the Tatarskii Strait with Amur River discharge and wind velocity over Amurskii Liman were also revealed.

Robust Pelvic Coordinate System Determination for Pose Changes in Multidetector-row Computed Tomography Images

  • Kobashi, Syoji;Fujimoto, Satoshi;Nishiyama, Takayuki;Kanzaki, Noriyuki;Fujishiro, Takaaki;Shibanuma, Nao;Kuramoto, Kei;Kurosaka, Masahiro;Hata, Yutaka
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • 제10권1호
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    • pp.65-72
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    • 2010
  • For developing navigation system of total hip arthroplasty (THA) and evaluating hip joint kinematics, 3-D pose position of the femur and acetabulum in the pelvic coordinate system has been quantified. The pelvic coordinate system is determined by manually indicating pelvic landmarks in multidetector-row computed tomography (MDCT) images. It includes intra- and inter-observer variability, and may result in a variability of THA operation or diagnosis. To reduce the variability of pelvic coordinate system determination, this paper proposes an automated method in MDCT images. The proposed method determines pelvic coordinate system automatically by detecting pelvic landmarks on anterior pelvic plane (APP) from MDCT images. The method calibrates pelvic pose by using silhouette images to suppress the affect of pelvic pose change. As a result of comparing with manual determination, the proposed method determined the coordinate system with a mean displacement of $2.6\;{\pm}\;1.6$ mm and a mean angle error of $0.78\;{\pm}\;0.34$ deg on 5 THA subjects. For changes of pelvic pose position within 10 deg, standard deviation of displacement was 3.7 mm, and of pose was 1.28 deg. We confirmed the proposed method was robust for pelvic pose changes.

Update on Distribution and Genetic Variability of Plum pox virus Strains in Bulgaria

  • Kamenova, Ivanka;Borisova, Anelija
    • The Plant Pathology Journal
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    • 제35권3호
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    • pp.243-256
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    • 2019
  • Field surveys for Plum pox virus (PPV) infection were conducted in stone fruit orchards all over Bulgaria. In total, 1168 out of 3020 leaf samples from cultivated Prunus spp. and wildly growing P. cerasifera trees reacted positive for PPV in DASI-ELISA with the universal monoclonal antibody (MAb) 5B. Further ELISA analyses showed that 987 and 127 isolates belonged to PPV-M and PPV-D serotypes, respectively. The plum and P. cerasifera showed 82.0% and 50.5% levels of infection, respectively followed by the peach (40.0%) and the apricot (32.0%). Five hundred fifty one PPV isolates were further typed by IC-RT-PCR with PPV-Rec, -M and -D-specific primers, targeting (Cter)NIb-(Nter) CP genome region, as 125 isolates were sequenced. The results revealed the presence of PPV-Rec, PPV-M and PPV-D and mixed infections of these strains. PPV-Rec was the most prevalent strain (49.0%), followed by PPV-M (40.1%), while PPV-D was the less spread strain (8.2%). PPV-Rec was the most common strain in plums, including the eight "old-aged" trees from the region of the first Sharka discovery. PPV-M was the most prevalent strain in peach and apricot. Phylogenetic analyses on (Cter)NIb-(Nter)CP of the isolates were performed. PPV-Rec isolates formed a homogeneous group, while PPV-M isolates split into PPV-Ma and PPV-Mb subgroups. Five separated clades were formed by the analyzed PPV-D isolates. Nucleotide sequences of the partial CP coding region of the analyzed isolates revealed a slightly higher intra-strain genetic variability in PPV-Rec and PPV-M isolates, while that of PPV-D strain isolates was higher from the reported for these strains.