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http://dx.doi.org/10.5423/PPJ.OA.11.2017.0253

Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP  

Kamran, Muhammad (Plant Pathology Research Institute, Ayub Agricultural Research Institute Faisalabad)
Javed, Nazir (Department of Plant Pathology, University of Agriculture Faisalabad)
Ullah, Ihsan (School of Agriculture, Policy and Development, University of Reading)
Nazir, Shahid (Agricultural Biotechnology Research Institute)
Jamil, Shakra (Agricultural Biotechnology Research Institute)
Iqbal, Muhammad Zafar (Agricultural Biotechnology Research Institute)
Abbas, Huma (Department of Plant Pathology, University of Agriculture Faisalabad)
Khan, Sajid Aleem (Department of Plant Pathology, University of Agriculture Faisalabad)
Haq, Muhammad Ehetisham ul (Plant Pathology Research Institute, Ayub Agricultural Research Institute Faisalabad)
Publication Information
The Plant Pathology Journal / v.35, no.1, 2019 , pp. 51-62 More about this Journal
Abstract
A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures ($25{\pm}2^{\circ}C$ and $30{\pm}2^{\circ}C$) and concentrations ($10^4$ and $10^5$ spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.
Keywords
attachment; bacterial parasite; genetic diversity; root knot nematode;
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