• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.457-466
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• 2024

Cellobiose dehydrogenases (CDHs) are a group of enzymes belonging to the hemoflavoenzyme group, which are mostly found in fungi. They play an important role in the production of acid sugar. In this research, CDH annotated from the actinobacterium Cellulomonas palmilytica EW123 (CpCDH) was cloned and characterized. The CpCDH exhibited a domain architecture resembling class-I CDH found in Basidiomycota. The cytochrome c and flavin-containing dehydrogenase domains in CpCDH showed an extra-long evolutionary distance compared to fungal CDH. The amino acid sequence of CpCDH revealed conservative catalytic amino acids and a distinct flavin adenine dinucleotide region specific to CDH, setting it apart from closely related sequences. The physicochemical properties of CpCDH displayed optimal pH conditions similar to those of CDHs but differed in terms of optimal temperature. The CpCDH displayed excellent enzymatic activity at low temperatures (below 30℃), unlike other CDHs. Moreover, CpCDH showed the highest substrate specificity for disaccharides such as cellobiose and lactose, which contain a glucose molecule at the non-reducing end. The catalytic efficiency of CpCDH for cellobiose and lactose were 2.05 × 10⁵ and 9.06 × 10⁴ (M^-1 s^-1), respectively. The result from the Fourier-transform infrared spectroscopy (FT-IR) spectra confirmed the presence of cellobionic and lactobionic acids as the oxidative products of CpCDH. This study establishes CpCDH as a novel and attractive bacterial CDH, representing the first report of its kind in the Cellulomonas genus.

• Journal of Korea Water Resources Association
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• v.53 no.spc1
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• pp.765-772
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• 2020

The amount of water resources identified by water balance analysis are usually used to formulate water resources plans. However, this does not consider the trade in goods between countries. It is possible to use virtual water to come up with a supply and demand plan by looking at the export and import of products. This is because it looks comprehensively at the direct water use (water resources within the region) and indirect water use (water resources of other regions from imported products). Yet South Korea does not actively use the concept of virtual water in the national water resources plan. There is difficulty with calculating and identifying the appropriate virtual water amount as many of the research papers present only few of the cases or omit the calculation process. This paper introduces detailed water footprint values for calculating the virtual water trade for South Korea. The international movement, water footprint, and virtual water trade of agricultural and livestock products are presented and compared to existing research. The water footprint and virtual water research in this paper can be utilized as baseline data for future researchers.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.27 no.3
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• pp.193-201
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• 2021

Patents for postharvest technology of fresh fruits, vegetables and mushrooms fluctuated in their filing number until the 2000s, but then there has been a steady increase in the 2010s, indicating that the technology has high growth potential in the international agricultural market. Using 551 valid patents for fresh fruits and 337 for fresh vegetables of 5 countries, patent trend of postharvest technology was analyzed in this study with two categorized technologies as packaging and storage. In Korea, patents from national research institutes such as the Korea Food Research Institute and Korea (Rural Development Administration) are dominated, and a number of patents for the postharvest storage of fresh vegetables by Mitsui Chemicals, a Japanese chemical company, have been registered. Agrofresh, a multinational company in the USA, holds a number of patents for postharvest storage of fresh fruit not only in its own country, but also in Japan and Europe. Postharvest technology of fresh fruits and vegetables was found to has a number of patents, mainly from national research institutes and multinational corporations, and this suggests that R&D and commercialization of the technologies are actively taking place.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.3
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• pp.175-178
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• 2005

The research was conducted to evaluate a different seedling establishment of rice germplasms which were obtained from the IRRI (International Rice Research Institute) under the direct seeded condition. There was highly significant difference in terms of statistical analysis among cultivars. The seedling establishment of cultivars tested was the highest with ASD1 > IR50 > IR72 > Taothabi > Uplri5 > CO25 > Dula > Moroberakan. The difference of seedling establishment among cultivars particular in dry condition was high between ASD1 and Moroberakan and other cultivars.

• International Journal of Industrial Entomology and Biomaterials
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• v.13 no.2
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• pp.51-55
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• 2006

Since 1970, China has become the biggest cocoon producer in the world, and made the highest historical record of cocoon output for 759,800 tons in 1995. However, in 1996 cocoon production reduced sharply to 470,900 tons. After a ten-year adjustment and reform, sericultural areas have shifted from developed regions to developing regions and from the east to the west. From 2000, the cocoon output has started to increase restoringly. By 2004 it recovered to 547,091 tons. With the development of market economy, sericulture management has been changed, including mulberry fields concentrated to the specializated households and cooperatives, cocoons produced in larger scale instead of individuals, Silkworm egg producing enterprises gradually changed into non-governmental joint-stock ones. The mechanism of market cocoon price has been gradually established. The management model of combination of trade, industry and agriculture is pushing and improving. It is the fruit of modern science and technology, especially sericultural basic research, that provides China's sericulture with the opportunity and vital force. China's sericulture, therefore, will continue to develop steadily in future.

• Journal of Climate Change Research
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• v.5 no.4
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• pp.313-321
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• 2014

Agriculture is a vulnerable sector to climate change because it is a primary industry directly exposed to climate. At the same time, it is a sector emitting greenhouse gases during agricultural activities, thereby affecting climate change. However, agriculture is a nascent subject under the UNFCCC. The agriculture sector needs both adaptation and mitigation. Currently, co-benefit of adaptation and mitigation is emphasized during climate change negotiation. Developing country parties are in a position to focus on adaptation rather than mitigation. As a result, mitigation is not being addressed enough during climate negotiation. Agriculture has been addressed through Nairobi Work Programme and NAPA. Since current efforts for greenhouse gas reduction are not sufficient for stabilizing the atmospheric system of the Earth to prevent climate change, the agriculture sector should also be considered for greenhouse gas reduction. For this, MRV for small farmers in developing countries and incentives for their mitigation efforts should be developed in agriculture sector. In addition, it is necessary to strengthen international cooperation for developing capacities for vulnerable countries and people to climate change.

• Advances in nano research
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• v.13 no.1
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• pp.29-45
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• 2022

The optimization for dynamic response associated with a cylindrical shell which is made of laminated composites embedded in a piezoelectric layer which is subjected to temperature rises and is resting on an elastic foundation is investigated for the first time. The first shear order theory (FSDT) is utilized in order to obtain the strain relations of the shell. Then, using the energy method, the equations of motions as well as boundary condition of the problem are attained. The formulation of this study together with the solution procedure which is a numerical solution method, differential quadrature method (DQM) is validated using other researches. This paper presents a thorough study on the parameters which impacts the vibration frequency of the laminated shell. The results of this paper shows that any type of laminated composite shell can reduce the vibration frequency providing that the angle related to layer are higher than 85 degrees. Also, in order to reduce the effect of temperature rises, the laminated composites instead of orthotropic one can be used.

• Food Science and Preservation
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• v.22 no.3
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• pp.369-376
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• 2015

This study was performed to develop the maufacturing processes of Makgeolli using red ginseng starch (RGS). After the fermentation of RGS with koji, nuruk, and yeast, the different temperature effects on the number of the yeast cells, the content of organic acid, free sugars, and total acid, and pH were investigated. There were no changes in the composition of the yeast cell number and content of organic acid amd during 20 days at $4^{\circ}C$. The content of free sugars (sucrose, glucose and mannose) and the pH value of red ginseng Makgeolli decreased during storage at $4^{\circ}C$. This meant that the total acid content and pH value increased after organic acid was produced from fermentation. Therefore, red ginseng Makgeolli is highly acidic and sour. Since high acidity helps improve storage conditions, so this developed red ginseng Makgeolli is considered safe for consumption. Furthermore, the total content of ginsenoside was 2.47 mg/mL, which was differentiate Makgeolli using red ginseng starch, with others. Therefore, new red ginseng Makgeolli is rich in organic acid, free sugars, and ginsenoside. As a result, its storage, taste, and flavor improved.

• Korean Journal of Breeding Science
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• v.40 no.1
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• pp.31-38
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• 2008

This experiment was carried out to improve the anther culture efficiency of barley (Hordeum vulgare L.). Callus induction rates from anther cultures of the five domestic naked barley and four unhulled varieties ranged from 0 to 5.6%, and plant regeneration rate to callus was 30.4% in the donor plants grown in a greenhouse during winter, among which the green plant regeneration rates ranged from 0 to 4.4%. Plant regeneration rate was 30.4% in the donor plants grown in a greenhouse during winter, whereas 21.3% in the normal field condition in spring. In addition, callus induction rates were 19.2% in plants grown in a normal field and 7.2% in drought-stressed condition, respectively. Being Considered the anther culture efficiency affected by the sampling time, the optimum sampling stage of anthers was 3~4 days before heading when the length between the 1st and 2nd auricles reaches 5 to 10 cm and at the uninucleate of pollen which the tip of the 2nd auricle aligns with the middle of panicle in the leaf sheath. Best callus induction rates came from the anthers stored at $4^{\circ}C$ for 3 weeks in a 10 to 15 cm diameter polyethylene bag with 5 to 10 panicles and Duwonchapssalbori and Saessalbori showed the higher induction rate of 4.8% and 1.7%, respectively.

• Korean Journal of Agricultural Science
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• v.38 no.2
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• pp.285-294
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• 2011

Glucosinolate (GSL) contents were investigated (i) at 1~7 days after sowing (DAS) in seed sprouts and (ii) at 3-7 weeks after sowing for the time-course. Moreover, (iii) They were compared with five different cultivars of rocket salad (Eruca sativa). Seventeen GSLs were separated by HPLC analysis, and 10 GSLs among them were identified as glucoraphanin, sinigrin, glucoalyssin, diglucothiobeinin, glucobrassicanapin, glucoerucin, glucobrassicin, dimeric, 4-mercaptobutyl GSL, 4-methoxy glucobrassicin, gluconasturttin by using LC-APCI-MS analysis, but 7 compounds were not identified. (i) The total GSL content in seed sprouts initially increased up to 3 DAS and then decreased according to their seedling growth. In particular, glucoraphanin known as a strong anti-cancer reagent was found the highest level (5.05 ${\mu}mol/g$ dry wt.) at 3 DAS. The most abundant GSL was glucoerucin ranged from 26.0~49.6 ${\mu}mol/g$ dry wt. (ii) In the time-course, the total GSL contents increased dramatically from 3-week (5.91 ${\mu}mol/g$ dry wt.) to 7-week after sowing (32.2 ${\mu}mol/g$ dry wt.). The major GSLs were glucoraphanin, glucoerucin and 4-methoxy glucobrassicin. (iii) By comparing GSL contents with five different cultivars, the total GSL contents increased from 4-week to 6-week after sowing, regardless of cultivar. In 4-week-old, the order with the total GSL content was "Rucola" > "Rocket Herbs" ${\geqq}$ "Odyssey" > "Takii" > "Herb", but in 6-week-old it is changed as "Takii" > "Herb" > "Odyssey" > "Rucola" > "Rocket Herbs" even there was almost no significantly difference between them.