• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.302-310
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• 2016

A study aimed at identifying putative drought responsive genes that confer tolerance to water stress deficit in tea plants was conducted in a 'rain-out shelter' using potted plants. Eighteen months old drought tolerant and susceptible tea cultivars were each separately exposed to water stress or control conditions of 18 or 34% soil moisture content, respectively, for three months. After the treatment period, leaves were harvested from each treatment for isolation of RNA and cDNA synthesis. The cDNA libraries were sequenced on Roche 454 high-throughput pyrosequencing platform to produce 232,853 reads. After quality control, the reads were assembled into 460 long transcripts (contigs). The annotated contigs showed similarity with proteins in the Arabidopsis thaliana proteome. Heat shock proteins (HSP70), superoxide dismutase (SOD), catalase (cat), peroxidase (PoX), calmodulinelike protein (Cam7) and galactinol synthase (Gols4) droughtrelated genes were shown to be regulated differently in tea plants exposed to water stress. HSP70 and SOD were highly expressed in the drought tolerant cultivar relative to the susceptible cultivar under drought conditions. The genes and pathways identified suggest efficient regulation leading to active adaptation as a basal defense response against water stress deficit by tea. The knowledge generated can be further utilized to better understand molecular mechanisms underlying stress tolerance in tea.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.1
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• pp.69-72
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• 2003

Development and life history of the Korean native firefly, Pyrocoelia rufa, were investigated throughout the indoor rearing. Average size of egg with an oval shape was 1.7 m and the hatchability of egg of P. rufa was approximately 88.5%. The larvae were pupated at the ${5^th}$ instar and the body length of the matured larvae was 27.1 mm. The total periods of larval stage to the end of the 5$^{th}$ instar were approximately 104.7 days. Average pupal period was 10.3 days and average number of eggs oviposited by a female was 87.3 eggs. The body size of female in the pupa and adult was larger than that of male. The wings of female adult were deteriorated.

• International Journal of Industrial Entomology and Biomaterials
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• v.13 no.2
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• pp.85-95
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• 2006

Seveval Chinese and Japanese varieties with good characters were used in the breeding. After 5 years (15 generations), a pair of robust and high quality silkworm variety with NPV resistance was bred by means of a combination of crossing and pedigree selection complemented by the selection of NPV resistance. The variety was identified jointly nationwide in 2003 and 2004, and appraised by National Mulberry and Silkworm Appraising Committee. Results are as follows: its cocooning rate is over 93%, shell rate 23-25%, filament length 1200-1300 meters, reelability 75-88%, Length of non-broken cocoon filament 900-1100 meters, raw silk rate 17-19%, neatness 95-97 points, and cocoon crop, cocoon shell weight and raw silk weight per 10000 larvae is higher than those of the control variety by 7-10%, 14-19% and 14-18%, respectively. The variety is not only robust, resistant to high temperature and NPV, easy to rear, uniform in hatching, molting and maturing, but also lays more eggs, and its fecundity is high. It is suitable to rear in the Yangtze River Basin, the Yellow River basin and the Pearl River basin of China.

• Journal of Veterinary Science
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• v.21 no.5
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• pp.80.1-80.13
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• 2020

Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments. Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID₅₀). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 ㎍/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log₁₀TCID₅₀ of 62.5 ㎍/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 ㎍/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. Conclusions: APB-13 exhibited good antiviral effects on TGEV in vivo and in vitro.

• International Journal of Industrial Entomology and Biomaterials
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• v.5 no.1
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• pp.135-139
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• 2002

By means of correlation analysis and path coefficient analysis, 18 characteristics of 325 conserved silkworm races were studied for the selecting efficiency on main silk quality characteristics. The result showed that selecting lines with heavier cocoon weight are beneficial to raise the weight of have. Choosing strains with higher cocoon shell percentage, longer filament length and better reelability percentage is good for increasing non-broken filament length. Selecting lines with medium daily weight of have produced in the fifth instar, medium cocoon shell percentage and simultaneously selecting heavier cocoon shell weight are favorable to promote reelability percentage. Choosing strains with heavier cocoon weight, higher cocoon shell percentage, heavier daily weight of bave produced in the fifth instar and heavier weight of bave but shorter filament length may hopefully breed out a race with thick filament size. And selecting strains with heavier weight of have and fine filament size are beneficial to increase filament length. Selecting lines with higher cocoon shell percentage and heavier weight of bave are favorable to raise raw silk percentage of cocoon. There are no significant direct or indirect correlation coefficient between viability and major characteristics of silk quality. It is, therefore, feasible to breed a silkworm race with excellent vitality and good silk quality as well.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.1
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• pp.129-135
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• 2011

Cathepsins are well-characterized proteases that are ubiquitously expressed in lysosomes. Previous work revealed that $Bombyx$ $mori$ cathepsins B and D are expressed in the fat body and undergo decomposition during larval-pupal metamorphosis. Quantitative RT-PCR was performed to detect cathepsin gene expression at the transcription level when challenged by $B.$ $mori$ nuclear polyhedrosis virus (BmNPV), temperature and hormones (20-hydroxyecdysone (20E) and juvenile hormone analogue (JHA)). mRNAs encoding cathepsins B and D were significantly enhanced after the larvae were infected with BmNPV, and the peak of the induction appeared at 1 day before spinning. This attenuated the inducing effect on cathepsin expression caused by infection. Temperature shock induced cathepsin expression at the later stage of the $5^{th}$ instar, and transcription levels varied with development stage and temperature. Cathepsin B and D mRNA expression in the fat body were significantly induced by JHA at the day before spinning, and with 20E, the expression reached a peak at the last day of the $5^{th}$ instar. Cathepsin B and D mRNA expression exhibited detectable changes post-treatment, without significant differences between or among the hormone concentrations.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.2
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• pp.197-201
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• 2003

The recombinant plasmids harboring a heterologous gene coding mouse endostatin were transfected and expressed stably in Trichoplusia ni Tn 5B1-4 cells and Bombyx mori BmN cells, respectively. Recombinant endostatin expressed in the stably transformed Tn 5B1-4 and BmN cells was secreted into the medium. BmN cells are relatively lower in maximum cell growth and recombinant endostatin production than Tn 5B 1-4 cells. Recombinant endostatin was also purified to homogeneity using a simple one-step ${Ni^2+}$ affinity fractionation method. Purified recombinant endostatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition $({ED_50})$ for recombinant endostatin was approximately 0.35 ${\mu}g$/ml.

• Food Science of Animal Resources
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• v.43 no.5
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• pp.723-750
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• 2023

The gut microbiome is critical in human health, and various dietary factors influence its composition and function. Among these factors, animal products, such as meat, dairy, and eggs, represent crucial sources of essential nutrients for the gut microbiome. However, the correlation and characteristics of livestock consumption with the gut microbiome remain poorly understood. This review aimed to delineate the distinct effects of meat, dairy, and egg products on gut microbiome composition and function. Based on the previous reports, the impact of red meat, white meat, and processed meat consumption on the gut microbiome differs from that of milk, yogurt, cheese, or egg products. In particular, we have focused on animal-originated proteins, a significant nutrient in each livestock product, and revealed that the major proteins in each food elicit diverse effects on the gut microbiome. Collectively, this review highlights the need for further insights into the interactions and mechanisms underlying the impact of animal products on the gut microbiome. A deeper understanding of these interactions would be beneficial in elucidating the development of dietary interventions to prevent and treat diseases linked to the gut microbiome.

• Journal of the Korean Institute of Gas
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• v.28 no.1
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• pp.1-10
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• 2024

In this study, we constructed a process simulation model for an agricultural by-products based Solid Oxide Fuel Cell (SOFC) combined heat and power generation system as part of the introduction of technology for energy self-sufficiency in the agricultural sector. The aim was to reduce the burden of increasing fuel and electricity consumption due to rapid fluctuations in international oil prices and the expansion of smart farming in domestic farms, while contributing to the national greenhouse gas reduction goals. Based on the experimental results of 0.3 ton/day torrefied agricultural by-product gasification experiment, a model for an agricultural by-product-based SOFC cogeneration system was constructed, and optimization of the heat exchange network was conducted for SOFC capacities ranging from 4 to 20 kW. The results indicated that an 8 kW agricultural by-product-based SOFC cogeneration system was optimal under the current system conditions. It is anticipated that these research findings can serve as foundational data for future commercial facility design.

• Journal of Species Research
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• v.12 no.1
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• pp.68-89
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• 2023

This is the second catalog listing unrecorded invertebrates discovered during the research project 'Discovery of Korean Indigenous Species'. The data to compile the catalog were primarily gathered from the final reports of the project, between 2013 and 2021. We present 38 previously undocumented species, belonging to four phyla (Platyhelminthes, Annelida, Rotifera and Arthropoda). Samples were collected from intertidal coastal waters, soil, freshwater ponds, reservoirs and hosts in South Korea. In this study, we provide brief taxonomic information, including collection site (GPS), diagnosis, specimen vouchers, figures of representative individuals and the Korean name newly assigned, for each species. All data were reviewed and updated by experts working on the respective taxonomic group. The aim of the present study is to publish species that have been previously reported through the project. Upon publication, these species will be added to the 'National Species List of Korea', curated by the National Institute of Biological Resources(NIBR).