Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Antiviral effects of Bovine antimicrobial peptide against TGEV in vivo and in vitro

  • Liang, Xiuli (College of Animal Science and Technology, Shihezi University) ;
  • Zhang, Xiaojun (Henan Joint International Research Laboratory of Veterinary Biologics Research and Application, Henan Provincial Animal Disease Prevention and Control and Nutrition Immunization Academician workstation, Anyang Institute of Technology) ;
  • Lian, Kaiqi (Henan Joint International Research Laboratory of Veterinary Biologics Research and Application, Henan Provincial Animal Disease Prevention and Control and Nutrition Immunization Academician workstation, Anyang Institute of Technology) ;
  • Tian, Xiuhua (Anyang County Agricultural and Rural Bureau) ;
  • Zhang, Mingliang (Henan Joint International Research Laboratory of Veterinary Biologics Research and Application, Henan Provincial Animal Disease Prevention and Control and Nutrition Immunization Academician workstation, Anyang Institute of Technology) ;
  • Wang, Shiqiong (College of Animal Husbandry, Henan Agricultural University) ;
  • Chen, Cheng (College of Animal Science and Technology, Shihezi University) ;
  • Nie, Cunxi (College of Animal Science and Technology, Shihezi University) ;
  • Pan, Yun (Henan Yihongshancheng Bio-Tech Co. Ltd) ;
  • Han, Fangfang (College of Animal Husbandry, Henan Agricultural University) ;
  • Wei, Zhanyong (College of Animal Husbandry, Henan Agricultural University) ;
  • Zhang, Wenju (College of Animal Science and Technology, Shihezi University)
  • Received : 2020.03.12
  • Accepted : 2020.08.11
  • Published : 2020.09.30
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Abstract

Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments. Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID50). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 ㎍/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log10TCID50 of 62.5 ㎍/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 ㎍/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. Conclusions: APB-13 exhibited good antiviral effects on TGEV in vivo and in vitro.

Keywords

Acknowledgement

This study was supported by funds from the National Key Research and Development Program of China (2017YFD0501003 and 2016YFD0500102).

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