Browse > Article

Comparative Study on Trichoplusia ni Tn 5B1-4 Cells and Bombyx mori BmN Cells for Recombinant Endostatin Production  

Sohn, Bong-Hee (Department of Sericultue and Entomology, The National Institute of Agricultural Science and Technology)
Lee, Jong-Min (Department of Genetic Engineering and Plant Metabolism Research Center, Kyung Hee University)
Kang, Pil-Don (Department of Sericultue and Entomology, The National Institute of Agricultural Science and Technology)
Lee, Sang-Uk (Department of Sericultue and Entomology, The National Institute of Agricultural Science and Technology)
Kim, Yong-Soon (Department of Sericultue and Entomology, The National Institute of Agricultural Science and Technology)
Chung, In-Sik (Department of Genetic Engineering and Plant Metabolism Research Center, Kyung Hee University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.7, no.2, 2003 , pp. 197-201 More about this Journal
Abstract
The recombinant plasmids harboring a heterologous gene coding mouse endostatin were transfected and expressed stably in Trichoplusia ni Tn 5B1-4 cells and Bombyx mori BmN cells, respectively. Recombinant endostatin expressed in the stably transformed Tn 5B1-4 and BmN cells was secreted into the medium. BmN cells are relatively lower in maximum cell growth and recombinant endostatin production than Tn 5B 1-4 cells. Recombinant endostatin was also purified to homogeneity using a simple one-step ${Ni^2+}$ affinity fractionation method. Purified recombinant endostatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition $({ED_50})$ for recombinant endostatin was approximately 0.35 ${\mu}g$/ml.
Keywords
Mouse endostatin; Trichoplusia ni Tn 5B1-4 cells; Bombyx mori BmN cells; In vitro activity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Boehm, T., S. Pirie-Shepherd, L. B. Trinh, J. Shiloach and J. Folkman (1999) Disruption of the KEXl gene in Pichiapas-toris allows expression of full-length murine and human endostatin. Yeast 15, 563-567   DOI   ScienceOn
2 Keith, M. B., P. J. Farrell, K. latrou and L. A. Behie (1999) Screening of transformed insect cell lines for recombinant protein production. Biotechnol. Prog. 15, 1046-1052   DOI   ScienceOn
3 Diaz-Flores, L., R. Gutierrez and H. Varela (1994) Angiogene-sis: an update. Histol. Histopath. 9, 807-843
4 Park, J. H., J. M. Lee and I. S. Chung (1999) Production of recombinant endostatin from stably transformed Drosophita melanosaster S2 cells. Biotechnol. Lett. 21, 729-733   DOI   ScienceOn
5 Finney, D. J. (1971) Probit analysis. 3rd edition, Cambridge, Cambridge University Press. U. K
6 Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of head ofbacteriophage. Nature 227, 680-685   DOI   PUBMED   ScienceOn
7 Kerbel, R. S. (1997) A cancer therapy resistant to resistance. Nature 390, 335-336   DOI   PUBMED   ScienceOn
8 Duan, J. B., X. Cai, B. L. Zhang, Y. Z. Li, M. J. Zou and J. X. Wang (1999) Efficient secretion of human endostatin in the yeast, Pichia pastoris. Biotechnol. Lett. 21, 1095-1099   DOI   ScienceOn
9 Park, J. H., K. H. Chang, J. M. Lee, Y. H. Lee and I. S. Chung (2001) Optimal production and in vitro activity of recombi-nant endostatin from stably transformed Drosophila melano-gaster S2 cells, In Vitro Cell. Dev. Biol. Animal 37, 5-9   DOI   ScienceOn
10 Blezinger, R, J. Wang, M. Gondo, A. Quezada, D. Mehrens, M. French, A. Singhal, S. Sullivan, A. Rolland, R. Ralston andW. Min (1999) Systemic inhibition of tumor growth and tumor metastases by intramuscular administration of the endostatin gene. Nature Biotechnol. 17, 343-348   DOI   ScienceOn
11 Farrell, P. J., M. Lu, J. Prevost, C. Brown, L. Behie and K. latrou (1998) High-level expression of secreted glycopro-teins in transformed lepidopteran insect cells using a novel expression vector. Biotechnol. Bioeng. 60, 654-663
12 O'Reilly, M. S., T. Boehm, Y. Shing, N. Fukai, G. Vasios, W. S. Lane, E. Flynn, J. R. Birkhead, B. R. Olsen and J. Folkman (1997) Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell 88, 277-285   DOI   ScienceOn
13 Boehm, T., J. Folkman, T. Browder and M. S. O'Reilly (1997) Antiangiogenic therapy of experimental cancer does not induce acquired drug resistance. Nature 390, 404-407   DOI   ScienceOn