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http://dx.doi.org/10.7852/ijie.2011.23.1.129

The Influence of Challenge on Cathepsin B and D Expression Patterns in the Silkworm Bombyx mori L.  

Wu, Feng-Yao (Jiangsu University of Science and Technology)
Zou, Feng-Ming (Jiangsu University of Science and Technology)
Jia, Jun-Qiang (Sericultural Research Institute, Chinese Academy of Agricultural Sciences)
Wang, Sheng-Peng (Sericultural Research Institute, Chinese Academy of Agricultural Sciences)
Zhang, Guo-Zheng (Sericultural Research Institute, Chinese Academy of Agricultural Sciences)
Guo, Xi-Jie (Sericultural Research Institute, Chinese Academy of Agricultural Sciences)
Gui, Zhong-Zheng (Sericultural Research Institute, Chinese Academy of Agricultural Sciences)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.23, no.1, 2011 , pp. 129-135 More about this Journal
Abstract
Cathepsins are well-characterized proteases that are ubiquitously expressed in lysosomes. Previous work revealed that $Bombyx$ $mori$ cathepsins B and D are expressed in the fat body and undergo decomposition during larval-pupal metamorphosis. Quantitative RT-PCR was performed to detect cathepsin gene expression at the transcription level when challenged by $B.$ $mori$ nuclear polyhedrosis virus (BmNPV), temperature and hormones (20-hydroxyecdysone (20E) and juvenile hormone analogue (JHA)). mRNAs encoding cathepsins B and D were significantly enhanced after the larvae were infected with BmNPV, and the peak of the induction appeared at 1 day before spinning. This attenuated the inducing effect on cathepsin expression caused by infection. Temperature shock induced cathepsin expression at the later stage of the $5^{th}$ instar, and transcription levels varied with development stage and temperature. Cathepsin B and D mRNA expression in the fat body were significantly induced by JHA at the day before spinning, and with 20E, the expression reached a peak at the last day of the $5^{th}$ instar. Cathepsin B and D mRNA expression exhibited detectable changes post-treatment, without significant differences between or among the hormone concentrations.
Keywords
$Bombyx$ $mori$; Cathepsin B and D; Expression; BmNPV; Hormones; Temperature;
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