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Molecular markers based on chloroplast and nuclear ribosomal DNA regions which distinguish Korean-specific ecotypes of the medicinal plant Cudrania tricuspidata Bureau

  • Lee, Soo Jin;Shin, Yong-Wook;Kim, Yun-Hee;Lee, Shin-Woo
    • Journal of Plant Biotechnology
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    • 제44권3호
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    • pp.235-242
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    • 2017
  • Cudrania tricuspidata Bureau is a widely-used, medicinal, perennial and woody plant. Obtaining information about the genetic diversity of plant populations is highly important with regard toconservation and germplasm utilization. Although C. tricuspidata is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish Korean-specific ecotypes from other ecotypes from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from the chloroplast and nuclear genomic sequences, which serve to to identify distinct Korean-specific ecotypes of C. tricuspidata via amplification refractory mutation system (ARMS)-PCR and high resolution melting (HRM) curve analyses. We performed molecular authentication of twelve C. tricuspidata ecotypes from different regions using DNA sequences in the maturaseK (MatK) chloroplast intergenic region and nuclear ribosomal DNA internal transcribed spacer (ITS) regions. The SNP markers developed in this study are useful for rapidly identifying specific C. tricuspidata ecotypes from different regions.

Podosphaera xanthii에 의한 좀돌팥 흰가루병 발생 (Occurence of Powdery Mildew Caused by Podosphaera xanthii on Vigna nakashimae in Korea)

  • 민경구;박태민;박윤진;장명준
    • 식물병연구
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    • 제28권4호
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    • pp.248-251
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    • 2022
  • 2022년 10월 충청남도 예산군(36°40'12.7"N, 126°51'36.4"E)에서 좀돌팥 흰가루병을 조사하였다. 병징은 주로 잎의 앞면에 발생하였으며 뒷면에는 흰가루병이 약하게 발병하였다. 흰가루병의 자낭구는 잎의 뒷면에서 확인하였다. 흰가루병균 무성세대 및 유성세대의 형태적 특징과 더불어 internal transcribed spacer rDNA, 28S large subunit 염기서열을 계통분석한 결과, 흰가루병균은 Podosphaera xanthii로 동정되었다. 이는 한국에서 Podosphaera xanthii에 의한 좀돌팥 흰가루병을 처음으로 보고한다.

치과위생사의 내적·외적만족과 조직몰입도의 관련성 (The correlation between the internal·external satisfaction and organizational commitment of dental hygienist)

  • 류혜겸
    • 한국치위생학회지
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    • 제17권5호
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    • pp.735-745
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    • 2017
  • Objectives: This study was conducted to provide a basic set of data on the correlation relationship between the internal external satisfaction and organizational commitment of dental hygienist. Methods: The research subjects were a total 264 numbers of dental hygienists in Busan and Gyeongnam. The study was conducted from July 1 to August 30, 2016. Structured questionnaires were used for analysis. The collected data was analyzed using IBM SPSS. Statistics 20.0. Results: Job satisfaction according to general characteristics showed high internal satisfaction and external satisfaction in age and prime duty, and internal satisfaction in career. The factors affecting organizational commitment were sub-factors of job satisfaction, external satisfaction and internal satisfaction. Conclusions: The atmosphere composition must be to improve that in the dental clinic, the dental hygienist's scope of work, autonomy, and sense of accomplishment through job the organizational commitment of the dental hygienists. This should be supported by the effective management of human resources, salary structure and the improvement of the system.

DEM analysis of the anisotropy effects on the failure mechanism of the layered concretes' specimens with internal notches

  • Jinwei Fu;Vahab Sarfarazi;Hadi Haeri;Mohammad Fatehi Marji
    • Computers and Concrete
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    • 제33권6호
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    • pp.659-670
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    • 2024
  • The mechanical behaviour of layered concrete samples containing an internal crack was numerically studied by modelling the geo-mechanical specimens in the particle flow code in two dimensions (PFC2D). The numerical modelling software was calibrated with the experimental results of the Brazilian tensile strengths gained from the laboratory disc-type specimens. Then, the samples with the bedding layers and internal notch were numerically simulated with PFC2D under uniaxial compressive loading. In each specimen, the layers' thickness was 10 mm but the layer's inclination angle was changed to 0°, 30°, 60°, 90°, 120° and 150°. Of course, the layers'interfaces are considered to have very low strengths. The internal notch was kept at 3 cm in length however, its inclination angle was changed to 0°, 40°, 60° and 90°. Therefore, a total, of 24 numerical models were made to study the failure mechanism of the layered concrete samples. Considering these results, it has been concluded that the inclination angles of both internal crack and bedding layers affect the failure mechanism and uniaxial compressive strength of the concrete.

전통지식 자원의 활용실태 연구 (A Study on the Actual Utilization of Traditional Knowledge Resources)

  • 김행란;최배영;유명님;김미희;강경하
    • 한국지역사회생활과학회지
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    • 제14권2호
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    • pp.93-106
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    • 2003
  • The main purpose of this research was to study and analyze the actual utilization of traditional knowledge resources and to search for methods to activate local communities through utilization of traditional knowledge resources best suited for us. For this study, data listed on the internal web sites during August 2002 to October 2002 were searched and analyzed. In terms of statistical analysis, frequency, percentage, and x$^2$-test were operated using the SPSS 10.0 program. The major results of this study are as follows: 1) Traditional knowledge resources utilized throughout the nation totaled to 8,906 cases. These utilized resources composed of 48.0% of tangible resources, 32.3% of environmental resources, and 19.8% of intangible resources and such utilized resources were in order of life-skill, scenery, ruins and relics, community activity, exhibition, and folklores. 2) Tourism, merchandising, and festival were the major types of utilization of traditional knowledge resources, while education was the relatively minor portion in utilization type. 3) Compound linking of traditional knowledge resources, utilization type, and utilizing body showed links such as life skill-merchandising-civilian, ruins and relics-tourism-government, folklore-festival-civilian, scenery-tourism-government, and exhibition-education-civilian.

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내부마케팅이 직무만족과 인지적 기업성과에 미치는 영향 : 한국수자원공사를 중심으로 (Internal Marketing Approach to Internal Satisfaction and Cognitive Organization Performance : The Case of Korean Water Resources Corporation)

  • 석영기
    • 한국콘텐츠학회논문지
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    • 제14권1호
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    • pp.374-385
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    • 2014
  • 본 연구의 목적은 한국수자원공사의 종업원 직무만족에 영향을 미치는 내부마케팅 요인 중 교육훈련, 보상제도, 의사소통, 권한위임의 선행변수가 직무만족과 인지적 기업성과에 미치는 영향을 파악하고, 내부 마케팅, 직무만족, 인지적 기업성과 등에 대한 문헌적 고찰과 한국수자원공사 내부고객을 대상으로 설문조사를 통한 실증분석을 실시하여 한국수자원공사 고객만족도 향상을 위한 시사점을 제시하는 것이다. 한국수자원공사의 직무만족을 설명하는 4개의 요인에 대한 로지스틱 회귀분석 결과, 교육훈련, 보상제도, 의사소통요인이 유의한 것으로, 권한위임은 유의하지 않은 것으로 나타났고, 인지적 기업성과의 경우, 교육훈련과 의사소통요인이 유의한 것으로 보상제도와 권한위임요인은 유의하지 않은 것으로 나타났다. 내부마케팅요인이 한국수자원공사의 성공에 적용될 수 있음을 알 수 있었다.

프란시스 수차 모델의 러너 간극에 따른 내부유동 및 성능 특성 (Internal Flow and Performance Characteristics According to the Runner Gap of a Francis Turbine Model)

  • 김승준;최영석;조용;최종웅;현정재;주원구;김진혁
    • 한국수소및신에너지학회논문집
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    • 제31권3호
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    • pp.328-336
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    • 2020
  • In the Francis turbine, the leakage flow through the runner gaps which are between the runner and the stator structure influences the internal flow and hydraulic performance. Thus, the investigation for the flow characteristics induced by the runner gaps is important. However, the runner gaps are often disregarded by considering the time and cost of the numerical analysis. Therefore, in this study, the flow characteristics according to runner gaps of the Francis turbine model were investigated including the leakage flow of the runner cone. The three-dimensional unsteady Reynolds-averaged Navier-Stokes analyses were conducted using a scale-adaptive simulation shear stress transport as a turbulence model for observing the influence of the leakage flow on the internal flow and hydraulic performance. The efficiencies were decreased slightly with runner gaps; and the complicated flows were captured in the gaps.

nrDNA ITS 및 엽록체 DNA 염기서열 분석에 의한 유통 한약재 오가피 판별 (Authentication of Traded Traditional Medicine Ogapi Based on Nuclear Ribosomal DNA Internal Transcribed Spacers and Chloroplast DNA Sequences)

  • 김정훈;변지희;박효섭;이정훈;이상원;차선우;조준형
    • 한국약용작물학회지
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    • 제23권6호
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    • pp.489-499
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    • 2015
  • Background : Plants belonging to 5 species of the genus Eleutherococcus are currently distributed in the Korean peninsula. The traditional medicine 'Ogapi', derived from Eleutherococcus sessiliflorus and other related species, and 'Gasiogapi', derived from Eleutherococcus senticosus, are frequently mixed up and marketed. Therefore, accurated identification of their origins in urgently required. Methods and Results : Candidate genes from nuclear ribosomal DNA (nrDNA) and chloroplast DNA (cpDNA) of Eleutherococcus plants were analyzed. Whereas the nrDNA-internal transcribed spacer (ITS) regions were useful in elucidating the phylogenetic relationships among the plants, the cpDNA regions were not as effective. Therefore, a combined analysis with nrDNA-ITS was performed. Various combinations of nrDNA and matK were effective for discriminating among the plants. However, the matK and rpoC1 combination was ineffective for discriminating among some species. Based on these results, it was found that OG1, OG4, OG5, OG7, GS1, GS2, and GS3 were derived from E. sessiliflorus. In particular, it was confirmed that GS1, GS2, and GS3 were not derived from E. senticosus. However, more samples need to be analyzed because identification of the origins of OG2, OG3, OG6 and GS4 was not possible. Conclusion : The ITS2, ITS5a, and matK combination was the most effective in identifying the phylogenetic relationship among Eleutherococcus plants and traditional medicines based on Eleutherococcus.

콘크리트 표면 차수벽형 석괴댐의 축조 중 침하거동 특성 - 대곡댐을 중심으로 - (Settlement Behavior Characteristics of CFRD in Construction Period - Case of Daegok Dam -)

  • 박한규;김용성;서민우;임희대
    • 한국지반공학회논문집
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    • 제21권7호
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    • pp.91-105
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    • 2005
  • 본 연구에서는 대곡댐의 계측자료에 의한CFRD의 축조에 따른 거동분석을 통하여 댐체가 받는 하중의 변화에 따른 침하 변형 거동을 분석하였으며, 외국의 CFRD 계측 결과와 비교 분석하였다. 대곡댐의 깊이에 따른 침하량 분석 결과, 전체적인 거동양상이 일반적인 CFRD 거동과 잘 일치하는 것으로 평가되었다. 또한, 38개 CFRD의 계측자료 분석 결과 변형계수, 간극비, 형상계수 등은 댐체의 침하 거동을 예측하는데 중요한 인자임을 확인하였으며 최대 층별 침하량이 작을수록 간극비는 낮게 나타났다. 최대 층별 침하량과 댐 높이의 관계에서 0.001에서 0.01사이에 상관계수를 갖는 댐이 26개로서, 일반적인 CFRD는 대략적으로 평균하였을 경우 최대 층별 침하량과 댐 높이는 0.005의 상관계수를 갖는 것으로 나타났다. 간극비가 낮은 경우 변형계수가 높았으며 형상계수는 4 이하로 나타났고 간극비가 높은 경우 상대침하율이 상대적으로 컸으며 형상계수도 4 이상으로 나타났다.

ISO 9001:2008 품질경영시스템 인증동기에 따른 요구사항 실행이 경영성과에 미치는 영향에 관한 연구 : 중소기업 제조업체를 중심으로 (A Study on the Effects of ISO 9001:2008 Quality Management Systems's Requirement Implementation According to the Certification Motives on Management Performance : Focused on the Manufacturer in the Small Businesses)

  • 김용준;정영배
    • 산업경영시스템학회지
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    • 제38권4호
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    • pp.1-10
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    • 2015
  • ISO 9001, quality management system, is widely known as advancing internal efficiency and productivity in various industry. As a result, the number of companies that have adopted ISO 9001's certification have been increasing continuously year by year since ISO 9000' family standard was published in 1987s. This fact shows that a lot of businesses are trying to get the accreditation for improving the competitiveness of quality. The purpose of this study is to present that ISO 9001's certification motives affect to the requirement's implementation and show that the execution of requirements influences on management performance in the small manufacturer. In order to carry out this research, we obtained 96 survey sheets and inspected twenty assumptions based on the reliability, validity of the questionnaires. The results of regression analysis are summarized as follows; First, the factors of certification motive influence on the requirement's implementation except resources management. Second, the two elements of ISO 9001 requirement, resources management and product realization, have an important effect on the operational performance and financial performance considerably in the companies that choose the internal motive. Finally, the two requisites of ISO 9001 requirement, resources management and measurement, analysis and improvement, affect to the operational performance and financial performance significantly in the businesses that select the external motive. Based on the results, the companies that choose the internal motive have a good performance to execute the ISO 9001 requirements. Also, to implement the requirements of ISO 9001 is helpful for the companies to produce the operational and financial performance.