Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Authentication of Traded Traditional Medicine Ogapi Based on Nuclear Ribosomal DNA Internal Transcribed Spacers and Chloroplast DNA Sequences

nrDNA ITS 및 엽록체 DNA 염기서열 분석에 의한 유통 한약재 오가피 판별

  • Kim, Jeong Hun (Department of Biological and Environmental Science, Dongguk University) ;
  • Byeon, Ji Hui (Department of Biological and Environmental Science, Dongguk University) ;
  • Park, Hyo Seop (Department of Biological and Environmental Science, Dongguk University) ;
  • Lee, Jeong Hoon (Department of Herb Crop Resources, NIHHS, RDA) ;
  • Lee, Sang Won (Department of Herb Crop Resources, NIHHS, RDA) ;
  • Cha, Sun Woo (Department of Herb Crop Resources, NIHHS, RDA) ;
  • Cho, Joon Hyeong (Department of Biological and Environmental Science, Dongguk University)
  • 김정훈 (동국대학교 바이오환경과학과) ;
  • 변지희 (동국대학교 바이오환경과학과) ;
  • 박효섭 (동국대학교 바이오환경과학과) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 조준형 (동국대학교 바이오환경과학과)
  • Received : 2015.10.01
  • Accepted : 2015.11.05
  • Published : 2015.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background : Plants belonging to 5 species of the genus Eleutherococcus are currently distributed in the Korean peninsula. The traditional medicine 'Ogapi', derived from Eleutherococcus sessiliflorus and other related species, and 'Gasiogapi', derived from Eleutherococcus senticosus, are frequently mixed up and marketed. Therefore, accurated identification of their origins in urgently required. Methods and Results : Candidate genes from nuclear ribosomal DNA (nrDNA) and chloroplast DNA (cpDNA) of Eleutherococcus plants were analyzed. Whereas the nrDNA-internal transcribed spacer (ITS) regions were useful in elucidating the phylogenetic relationships among the plants, the cpDNA regions were not as effective. Therefore, a combined analysis with nrDNA-ITS was performed. Various combinations of nrDNA and matK were effective for discriminating among the plants. However, the matK and rpoC1 combination was ineffective for discriminating among some species. Based on these results, it was found that OG1, OG4, OG5, OG7, GS1, GS2, and GS3 were derived from E. sessiliflorus. In particular, it was confirmed that GS1, GS2, and GS3 were not derived from E. senticosus. However, more samples need to be analyzed because identification of the origins of OG2, OG3, OG6 and GS4 was not possible. Conclusion : The ITS2, ITS5a, and matK combination was the most effective in identifying the phylogenetic relationship among Eleutherococcus plants and traditional medicines based on Eleutherococcus.

Keywords

References

  1. CBOL Plant Working Group. (2009). A DNA barcode for land plants. Proceedings of the National Academy of Science of the United States of America. 106:12794-12797. https://doi.org/10.1073/pnas.0905845106
  2. Deyama T, Nishibe S and Nakazawa Y. (2001). Constituents and pharmacological effects of Eucommia and siberian ginseng. Acta Pharmacologica Sinica. 22:1057-1070.
  3. Felsenstein J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 39:783-791. https://doi.org/10.2307/2408678
  4. Han HS, Kim DY, Lee KY, Park WG, Cho IK and Jung JS. (2006). Comparative analysis of Acanthopanax senticosus Harms from Korea, China and Russia based on the ITS sequences of nuclear ribosomal DNA. Korean Journal of Plant Resources. 19:54-58.
  5. Heo SJ, Ahn HY, Kang MJ, Lee JH, Cha JY and Cho YS. (2011). Antioxidative activity and chemical characteristics of leaves, roots, stems and fruits extracts from Acanthopanax senticosus. Journal of Life Science. 21:1052-1059. https://doi.org/10.5352/JLS.2011.21.7.1052
  6. Hollingsworth PM, Graham SW and Little DP. (2011). Choosing and using a plant DNA barcode. PLoS ONE. 6:e19254. https://doi.org/10.1371/journal.pone.0019254
  7. Hong KN, Cho KJ, Park YH, Hur SD, Hong YP and Kang BY. (2000). Genetic variation of some patches of Eleutherococcus senticosus(Rupr. and Maxim.) Maxim. in Korea. Journal of Korean Forestry Society. 89:645-654.
  8. Huh MK, Choi YH, Choi BT and Kim GC. (2005). Discrimination and genetic diversity of Acanthopanax senticosus using RAPD markers. Korean Journal of Oriental Physiology and Pathology. 19:1046-1049.
  9. Kim CH. (1997). Systematics of Eleutherococcus and related genera(Araliaceae). Ph. D. Thesis. Chonbuk National University. p.1-263.
  10. Kim HS, Han HS and Lee YJ. (2008). A study on a morphological identification of Acanthopanacis cortex. Korea Journal of Herbology. 23:41-49.
  11. Kim JH, Seo JW, Byeon JH, Ahn YS, Cha SW and Cho JH. (2014). Morphological characteristics and phylogenetic analysis of Polygonatum species based on chloroplast DNA sequences. Korean Journal of Medicinal Crop Science. 22:489-496. https://doi.org/10.7783/KJMCS.2014.22.6.489
  12. Kim MS, Sung CK, Kim HC, Gal SW and Lee SW. (2009). Physicochemical composition of the Acanthopanax chilsanensis. Journal of Life Science. 19:1815-1820. https://doi.org/10.5352/JLS.2009.19.12.1815
  13. Kim NS, Kwon J, Koh HY, Choi DS and Oh CH. (2004). Immunoregulatory action of Ogapi. Korean Journal of Oriental Physiology and Pathology. 18:1337-1342.
  14. Kim S, Kim KY, Park MS, Choi SY and Yun SJ. (1998). Intraspecific relationship of Eleutherococcus senticosus Max. by RAPD markers. Korean Journal of Medicinal Crop Science. 6:165-169.
  15. Kim YJ, Park MS, Park HK, Kim S and Sung CK. (1996). Eleutheroside E content in Eleutherococcus spp. Korean Journal of Medicinal Crop Science. 4:333-339.
  16. Kim YJ, Park HK, Park MS, Kim S and Choi KG. (1997). Morphological and anatomical characteristics of five Eleutherococcus species. Korean Journal of Breeding Science. 29:56-63.
  17. Lee CB. (2006). Coloured Flora of Korea I. Hyangmunsa. Seoul, Korea. p.811-813.
  18. Li M, Cao H, But PPH and Shaw PC. (2011). Identification of herbal medicinal materials using DNA barcodes. Journal of Systematics and Evolution. 49:271-283. https://doi.org/10.1111/j.1759-6831.2011.00132.x
  19. Lim JD, Seong ES, Choi KJ, Kim SK, Kim MJ and Yu CY. (2000). Intraspecific relationship analysis of Eleutherococcus senticosus Max. by RAPD markers. Korean Journal of Plant Resources. 13:104-110.
  20. Lim JM, Ahn YS, Park CG, Park CB and Cho JH. (2012). Authentication of traded medicinal herb, Glycyrrhiza spp. (Licorice), based on nrDNA-ITS2 sequence analysis. Journal of the Korean Society of International Agriculture. 24:435-443.
  21. Lim JM, Kim MS, Byeon JH, Park HS, Ahn YS, Park CG and Cho JH. (2013). Classification and discrimination of geographical origin of Bang-Poong[Saposhnikovia divaricata (Turcz) Schischkin] medicinal plant and related species by using DNA sequence analysis. Journal of the Korean Society of International Agriculture. 25:395-405. https://doi.org/10.12719/KSIA.2013.25.4.395
  22. Lim SH, Park YH, Kwon CJ, Ham HJ, Jeong HN, Kim KH and Ahn YS. (2010). Anti-diabetic and hypoglycemic effect of Eleutherococcus spp. Journal of the Korean Society of Food Science and Nutrition. 39:1761-1768. https://doi.org/10.3746/jkfn.2010.39.12.1761
  23. Little DP. (2014). A DNA mini-barcode for land plants. Molecular Ecology Resources. 14:437-446. https://doi.org/10.1111/1755-0998.12194
  24. Ma HL, Zhu ZB, Zhang XM, Miao YY and Guo QS. (2014). Species identification of the medicinal plant Tulipa edulis (Liliaceae) by DNA barcode marker. Biochemical Systematics and Ecology. 55:362-368. https://doi.org/10.1016/j.bse.2014.03.038
  25. Ministry of Food and Drug Safety(MFDS). (2013). The Korean herbal pharmacopoeia(4th ed.). Ministry of Food and Drug Safety. Seoul, Korea. p.157-158.
  26. Ministry of Food and Drug Safety(MFDS). (2015). The Korean pharmacopoeia(10th ed.). Ministry of Food and Drug Safety. Seoul, Korea. p.1839.
  27. Saitou N and Nei M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 4:406-425.
  28. Tamura K, Nei M and Kumar S. (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences of the USA. 101:11030-11035. https://doi.org/10.1073/pnas.0404206101