• Journal of Ginseng Research
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• 제45권4호
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• pp.465-472
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• 2021

Background: Ginseng can help regulate brain excitability, promote learning and memory, and resist cerebral ischemia in the central nervous system. Ginsenosides are the major effective compounds of Ginseng, but their protein targets in the brain have not been determined. Methods: We screened proteins that interact with the main components of ginseng (ginsenosides) by affinity chromatography and identified the 14-3-3 ζ protein as a potential target of ginsenosides in brain tissues. Results: Biolayer interferometry (BLI) analysis showed that 20(S)-protopanaxadiol (PPD), a ginseng saponin metabolite, exhibited the highest direct interaction to the 14-3-3 ζ protein. Subsequently, BLI kinetics analysis and isothermal titration calorimetry (ITC) assay showed that PPD specifically bound to the 14-3-3 ζ protein. The cocrystal structure of the 14-3-3 ζ protein-PPD complex showed that the main interactions occurred between the residues R56, R127, and Y128 of the 14-3-3 ζ protein and a portion of PPD. Moreover, mutating any of the above residues resulted in a significant decrease of affinity between PPD and the 14-3-3 ζ protein. Conclusion: Our results indicate the 14-3-3 ζ protein is the target of PPD, a ginsenoside metabolite. Crystallographic and mutagenesis studies suggest a direct interaction between PPD and the 14-3-3 ζ protein. This finding can help in the development of small-molecular compounds that bind to the 14-3-3 ζ protein on the basis of the structure of dammarane-type triterpenoid.

• 한국수자원학회논문집
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• 제55권12호
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• pp.1031-1039
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• 2022

강우는 산사태와 밀접한 연관성을 지니는 수문 인자 중 하나로, 외력을 발생시켜 지반 안정성에 간접적으로 관여한다. 최근에는 지반 안정성에 직접적인 영향을 미치는 인자인 지하수위의 변동에 대한 관심이 증가하고 있다. 이에 본 연구에서는 강우, 지하수위와 지반 변위의 변동을 통해 변수간 관계성을 분석하고자 하였다. PSInSAR 기법을 사용하여 도심과 산지에서의 지반 변위 모의 결과를 도출하였고, 위성 기반 강우와 지점 기반 지하 수위 자료와 연계하여 시계열 기반 분석을 실시하였다. 고강도 강우에 대해 도심과 산지에서 모두 급격한 지하수위의 상승이 나타났으며, 무강우 및 저강우 기간에 대해 도심에서는 지속적인 감소 경향이 나타났다. 지하수위의 증가에 대해 산지에서는 6 mm 수준의 지반 침하가 관측되었으며, 이는 표토층의 토사 유출로 인한 결과로 보인다. 한편 도심에서는 다양한 결과가 나타났는데 지하수위 상승에 대한 지반 변위의 증가와 토양 뼈대의 확장으로 인한 결과로 볼 수 있으나, 지반 변위의 감소는 인위적 영향에서 기인된 결과로 보인다. 전반적으로 지하수위와 지반 변위 간 관계성이 일관되게 나타나지 않았는데, 이는 수문인자 외의 다른 영향인자로 인한 결과로 보인다. 두 인자 간 관계성에 영향을 미치는 환경 인자들이 추가로 고려된다면 관계성을 보다 면밀히 파악하는 데 기여할 수 있을 것으로 보인다.

• 한국측량학회지
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• 제40권4호
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• pp.315-324
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• 2022

일반적인 조정계산에서는 독립변수의 오차는 없다고 가정하고 종속변수의 오차만을 고려하는 최소제곱해를 구한다. 그러나 지상측량에 의해 결정한 3차원 공간좌표나 GNSS (Global Navigation Satellite System) 기반 추정좌표는 성분별로 독립적으로 결정되지 않으므로 모든 성분에 오차가 있을 뿐만 아니라 공분산도 존재한다. 따라서 좌표쌍을 이용한 평면 추정이나 좌표계 변환에서는 모든 성분의 오차를 고려하는 전최소제곱을 적용해야 한다. 이를 위한 다양한 모델이 존재하며, 특별한 제약조건을 제외하면 동등한 해를 제공한다. 본 연구에서는 가우스-헬머트 모델(GHM: Gauss-Helmert Model) 기반 전최소제곱으로 VLBI 타겟이 형성하는 자취를 이용하여 평면의 법선벡터를 추정했으며, 지역좌표계를 세계측지계로 변환하는 계수 결정에도 적용했다. 평면방정식의 경우 기존 최소제곱 방법과 비교해서 법선벡터는 동일하지만 분산요소의 안정성과 타겟 위치에 따른 분산요소 특성을 명확히 확인할 수 있었다. 좌표계 변환계수는 가우스-헬머트 모델을 적용하면 변환 전후 두 좌표계에서 모두 잔차를 계산할 수 있으며, 기존 방식보다 잔차가 더 작아진다.

• Journal of Ginseng Research
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• 제46권5호
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• pp.666-674
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• 2022

Background: Ginsenosides and their metabolites have antidepressant-like effects, but the underlying mechanisms remain unclear. We previously identified 14-3-3 ζ as one of the target proteins of 20 (S)-protopanaxadiol (PPD), a fully deglycosylated ginsenoside metabolite. Methods: Corticosterone (CORT) was administered repeatedly to induce the depression model, and PPD was given concurrently. The tail suspension test (TST) and the forced swimming test (FST) were used for behavioral evaluation. All mice were sacrificed. Golgi-cox staining, GSK 3β activity assay, and Western blot analysis were performed. In vitro, the kinetic binding analysis with the Biolayer Interferometry (BLI) was used to determine the molecular interactions. Results: TST and FST both revealed that PPD reversed CORT-induced behavioral deficits. PPD also ameliorated the CORT-induced expression alterations of hippocampal Ser9 phosphorylated glycogen synthase kinase 3β (p-Ser9 GSK 3β), Ser133 phosphorylated cAMP response element-binding protein (p-Ser133 CREB), and brain-derived neurotrophic factor (BDNF). Moreover, PPD attenuated the CORT-induced increase in GSK 3β activity and decrease in dendritic spine density in the hippocampus. In vitro, 14-3-3 ζ protein specifically bound to p-Ser9 GSK 3β polypeptide. PPD promoted the binding and subsequently decreased GSK 3β activity. Conclusion: These findings demonstrated the antidepressant-like effects of PPD on the CORT-induced mouse depression model and indicated a possible target-based mechanism. The combination of PPD with the 14-3-3 ζ protein may promote the binding of 14-3-3 ζ to p-GSK 3β (Ser9) and enhance the inhibition of Ser9 phosphorylation on GSK 3β kinase activity, thereby activating the plasticity-related CREBeBDNF signaling pathway.

• 한국광학회지
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• 제34권3호
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• pp.117-123
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• 2023

반사경의 광학면이 넓어질수록 높은 공간 주파수 형상이 광학계의 성능에 큰 영향을 주므로, 대형 반사경 성능 평가에서 이를 측정할 수 있어야 한다. 따라서 높은 주파수의 형상을 샘플링할 수 있는 고해상도 형상 측정 시스템이 필요하다. 본 연구에서는 반사경의 고해상도 형상 측정을 위한 회전 프리즘 정합 간섭계(rotational prism stitching interferometer, RPSI)라는 새로운 방법을 제안한다. RPSI는 기존에 사용되고 있는 상용 간섭계에 빔 확장 렌즈, 이송 거울, 회전 프리즘 등을 추가하여 구성되었으며, 간섭계를 움직이지 않고 원통 좌표계를 기준으로 전체 구경을 아우르는 부분 구경 형상들을 측정한다. 측정된 부분 구경들은 최소자승법을 이용한 부분 구경 정합법을 이용하여 정합되며, 빔확장 렌즈 배율의 제곱만큼 높은 샘플링 밀도로 전체 구경 형상 정보를 복원 가능하다. 3배율을 가지는 RPSI를 이용하여 직경 40 mm의 구면 반사경 형상을 측정하였고, 간섭계 단독으로 측정한 전체 구경 형상과 비교 검증하였다. 약 1 nm RMS의 근소한 차이로 유사한 형상을 잘 복원할 수 있었으며, 간섭계 단독으로 사용한 형상 측정 결과보다 렌즈의 배율의 제곱배만큼 향상된 샘플링 밀도로 형상 측정이 가능함을 확인하였다.

• 천문학회지
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• 제56권2호
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• pp.231-252
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• 2023

We present the analysis results of the simultaneous multifrequency observations of the blazar 4C +28.07. The observations were conducted by the Interferometric Monitoring of Gamma-ray Bright Active Galactic Nuclei (iMOGABA) program, which is a key science program of the Korean Very Long Baseline Interferometry (VLBI) Network (KVN). Observations of the iMOGABA program for 4C +28.07 were conducted from 16 January 2013 (MJD 56308) to 13 March 2020 (MJD 58921). We also used γ-ray data from the Fermi Large Array Telescope (Fermi-LAT) Light Curve Repository, covering the energy range from 100 MeV to 100 GeV. We divided the iMOGABA data and the Fermi-LAT data into five periods from 0 to 4, according to the prosody of the 22 GHz data and the presence or absence of the data. In order to investigate the characteristics of each period, the light curves were plotted and compared. However, a peak that formed a hill was observed earlier than the period of a strong γ-ray flare at 43-86 GHz in period 3 (MJD 57400-58100). Therefore, we assumed that the minimum total CLEANed flux density for each frequency was quiescent flux (S_q) in which the core of 4C +28.07 emitted the minimum, with the variable flux (S_var) obtained by subtracting S_q from the values of the total CLEANed flux density. We then compared the variability of the spectral indices (α) between adjacent frequencies through a spectral analysis. Most notably, α_22-43 showed optically thick spectra in the absence of a strong γ-ray flare, and when the flare appeared, α_22-43 became optically thinner. In order to find out the characteristics of the magnetic field in the variable region, the magnetic field strength in the synchrotron self-absorption (B_SSA) and the equipartition magnetic field strength (B_eq) were obtained. We found that B_SSA is largely consistent with B_eq within the uncertainty, implying that the SSA region in the source is not significantly deviated from the equipartition condition in the γ-ray quiescent periods.

• Journal of Ginseng Research
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• 제48권2호
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• pp.202-210
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• 2024

Background: Panax ginseng Meyer polysaccharides exhibit various biological functions, like antagonizing galectin-3-mediated cell adhesion and migration. Galectin-8 (Gal-8), with its linker-joined N- and C-terminal carbohydrate recognition domains (CRDs), is also crucial to these biological processes, and thus plays a role in various pathological disorders. Yet the effect of ginseng-derived polysaccharides in modulating Gal-8 function has remained unclear. Methods: P. ginseng-derived pectin was chromatographically isolated and enzymatically digested to obtain a series of polysaccharides. Biolayer Interferometry (BLI) quantified their binding affinity to Gal-8, and their inhibitory effects on Gal-8 was assessed by hemagglutination, cell migration and T-cell apoptosis. Results: Our ginseng-derived pectin polysaccharides consist mostly of rhamnogalacturonan-I (RG-I) and homogalacturonan (HG). BLI shows that Gal-8 binding rests primarily in RG-I and its β-1,4-galactan side chains, with sub-micromolar K_D values. Both N- and C-terminal Gal-8 CRDs bind RG-I, with binding correlated with Gal-8-mediated function. Conclusion: P. ginseng RG-I pectin β-1,4-galactan side chains are crucial to binding Gal-8 and antagonizing its function. This study enhances our understanding of galectin-sugar interactions, information that may be used in the development of pharmaceutical agents targeting Gal-8.

• Journal of Ginseng Research
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• 제47권5호
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• pp.662-671
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• 2023

Background: 20(S)-protopanaxadiol (PPD), a ginsenoside metabolite, has prominent benefits for the central nervous system, especially in improving learning and memory. However, its transcriptional targets in brain tissue remain unknown. Methods: In this study, we first used mass spectrometry-based drug affinity responsive target stability (DARTS) to identify the potential proteins of ginsenosides and intersected them with the transcription factor library. Second, the transcription factor PURA was confirmed as a target of PPD by biolayer interferometry (BLI) and molecular docking. Next, the effect of PPD on the transcriptional levels of target genes of PURA in brain tissues was determined by qRT-PCR. Finally, bioinformatics analysis was used to analyze the potential biological features of these target proteins. Results: The results showed three overlapping transcription factors between the proteomics of DARTS and transcription factor library. BLI analysis further showed that PPD had a higher direct interaction with PURA than parent ginsenosides. Subsequently, BLI kinetic analysis, molecular docking, and mutations in key amino acids of PURA indicated that PPD specifically bound to PURA. The results of qRT-PCR showed that PPD could increase the transcription levels of PURA target genes in brain. Finally, bioinformatics analysis showed that these target proteins were involved in learning and memory function. Conclusion: The above-mentioned findings indicate that PURA is a transcription target of PPD in brain, and PPD upregulate the transcription levels of target genes related to cognitive dysfunction by binding PURA, which could provide a chemical and biological basis for the study of treating cognitive impairment by targeting PURA.

• 대한치과보철학회지
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• 제41권6호
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• pp.720-731
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• 2003

Statement of problem. The importance of fixture design and surface treatment. Purpose. The clinical success of dental in plants is affected by many factors such like as degree of osseointegration, the effective load dispersion for the prostheses, and a lot of attempts have been made to overcome the difficulties. In this study, efforts were made to find the possibility of clinical acceptance of the dental implants of newly designed surface and resorbable blast media surcace. Materials and methods. In this study, two groups of custom-made, screw-shaped implants were prepared. The first with the consisting of Branemark clone design and the other with the new design. These implants were divided into four groups according to the kinds of surface treatment. Four implants($AVANA^{(R)}$, Osstem, Busan, Korea)of each group were installed in twenty rabbits. Group A was consisted of Branemark done implant left as machined, Group B with Branemark clone implants with RBM(Resorbable blast media) surface, Group C with newly designed implants left as machined and Group D with newly designed implants with RBM surface. One of the twenty rabbits died from inflammation and the observation was made for six weeks. Specimens from four groups were observed using scanning electron microscopy with 40, 100, 1000 magnification power and microsurface structures were measured by white-light scanning interferometry for three dimensional surface roughness measurements(Accura $2000^{(R)}$, Intek-Plus, Korea.). Removal torque was measured in 17 rabbits using digital torque gauge(MGT 12R, Mark-10 corp., NY, U.S.A.) immediately after the sacrifice and two rabbits were used for the histologic preparation(EXAKT $310^{(R)}$, Heraeus Kulzer, wehrheim, Germany) of specimens and observed under light microscope. Resonance frequency measurement($Osstell^{(R)}$) was taken with the 19 rabbits at the beginning of the implant fixation and immediately after the sacrifice. Results. Following results were taken from the experiment. 1. The surface of the RBM implants as seen with SEM had rough and irregular pattern with reticular formation compared to that of fumed specimens showing different surface topographies. 2. The newly designed implant with RBM surface had high removal torque value among four groups with no statistical significance. The average removal torque was $49.95{\pm}6.70Ncm$ in Group A, $51.15{\pm}4.40Ncm$ in Group B, $50.78{\pm}9.37Ncm$ in Group C, $51.09{\pm}4.69Ncm$ in Group D. 3. The RFA values were $70.8{\pm}4.3Hz$ in Group A, $71.8{\pm}3.1Hz$ in Group B, $70.9{\pm}2.5Hz$, $72.7{\pm}2.5Hz$ in Group D. Higher values were noted in the groups which had surface treatment compared to the untreated groups with no statistical significance. 4. The results from the histomorphometric evaluation showed a mean percentage of bone-to-implant contact of $45{\pm}0.5%$ in Group A, $55{\pm}3%$ in Group B, $49.5{\pm}0.5%$ in Group C, and $55{\pm}3%$ in Group D. Quite amount of newly formed bone were observed at the surface RBM-treated implants in bone marrow space.

• 대한원격탐사학회지
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• 제34권6_4호
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• pp.1479-1488
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• 2018

2017년 9월 3일 북한에서 발생한 인공지진 신호가 기상청 지진관측망에 관측되었다. 진앙은 풍계리 핵실험 지역으로 지금까지의 실험 중 가장 강력한 실험이었다. 직접적 접근이 제한되는 상황에서 6차 핵실험에 의한 주변 지표변화 연구를 위해 고해상도 위성 자료를 활용하였다. 우선, 지표변위 관측을 위해 ALOS-2 위성 자료를 활용하여 레이더 간섭기법(InSAR: SAR Interferometry)을 적용하였다. 하지만 6차 핵실험 주변 지역의 대규모 지표변위와 강한 진동으로 인해 낮은 긴밀도(coherence) 값을 지니며 레이더 간섭도가 생성되지 않았다. 이는 강한 진동으로 인한 표면의 변화와 레이더 간섭도가 생성 가능한 최대 지표변위 관측 범위보다 큰 변위가 발생했기 때문으로 추정되며 이러한 한계를 극복하기 위해 오프셋 트래킹(Offset Tracking) 방법을 활용하였다. 오프셋 트래킹 방법은 6차 핵실험 전 후 위성 영상레이더의 강도 영상(Intensity)에 대한 교차상관기법(Cross-Correlation)을 이용하는 것으로 상관관계 추정을 위해 사용된 윈도우 크기에 따라 결과가 달라지는 단점이 존재한다. 본 연구에서는 32부터 224까지 16단계로 윈도우 크기를 변화시키고 그 결과의 통계적 처리 후 지표변위를 생성하였다. 그 결과, 6차 핵실험 장소를 기준으로 만탑산 서쪽 지역에서 최대 3 m의 지표변위를 관측하였다. 또한, 고해상도 광학 영상을 활용하여 6차 핵실험에 의한 산사태 및 함몰지역으로 추정되는 지역을 확인하였다. 이러한 현상은 매우 강력한 지하 핵실험에 의한 것으로 판단되며 기존 음파 및 지진파를 이용한 핵실험 분석뿐만 아니라 고해상도 위성영상을 활용하여 비접근 지역에 대한 보조 분석 자료로 활용이 가능 할 것이다.