• Preventive Nutrition and Food Science
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• v.8 no.4
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• pp.390-394
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• 2003

In the post-genome period, the technique for identifying gene expression has been progressed to high throughput screening. In the field of molecular nutrition, the use of screening techniques to clarify molecular function of specific nutrients would be very advantageous. In this study, we have evaluated Zn-regulated gene expression in Zn-deficient, homocystein-treated EA.hy926 cells, using cDNA microarray, which can be used to screen the expression of many genes simultaneously. The information obtained can be used for preliminary assessment of molecular and signaling events modulated by Zn under pro-atherogenic conditions. EA.hy926 cells derived from human umbilical vein endothelial cells were cultured in Zn-adequate (control, 15 $\mu$M Zn) or Zn-deficient (experimental, 0 $\mu$M Zn) Dulbecco's MEM media under high homocysteine level (100 $\mu$M) for 3 days of post-confluency. Cells were harvested and RNA was extracted. Total RNA was reverse-transcribed and the synthesized cDNA was labeled with Cy3 or Cy5. Fluorescent labeled cDNA probe was applied to microarray slides for hybridization, and the slide was then scanned using a fluorescence scanner. The expression of seven genes was found to be significantly decreased, and one significantly increased, in response to treatment of EA.hy926 cells with Zn-deficient medium, compared with Zn-supplemented medium. The upregulated genes were oncogenes and tumor suppressor genes, cell cycle-related genes and transporter genes. The down-regulated gene was RelB, a component of the NF-kappaB complex of transcription factors. The results of this study imply the effectiveness of cDNA microarray for expression profiling of a singly nutrient deficiency, namely Zn. Furthur study, using tailored-cDNA array and vascular endothelial cell lines, would be beneficial to clarify the molecular function of Zn in atherosclerosis, more in detail.

• The Journal of the Korea Contents Association
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• v.18 no.6
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• pp.434-442
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• 2018

Recently, the threat to the security and damage of important data leaked by devices of intranet infected by malicious code through the Internet have been increasing. Therefore, the partitioned intranet model that blocks access to the server for business use by implementing authentication of devices connected to the intranet is required. For this, logical net partition with the VDI(Virtual Desktop Infrastructure) method is no information exchange between physical devices connected to the intranet and the virtual device so that it could prevent data leakage and improve security but it is vulnerable to the attack to expose internal data, which has access to the server for business connecting a nonregistered device into the intranet. In order to protect the server for business, we suggest a blockchain based network partition model applying blockchain technology to VDI. It contributes to decrease in threat to expose internal data by improving not only capability to verify forgery of devices, which is the vulnerability of the VDI based logical net partition, but also the integrity of the devices.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.10 no.3
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• pp.145-152
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• 2010

With the recent development of the ubiquitous computing technology, there are increasing interest and research in technologies such as sensors and RFID related to information recognition and location positioning in various ubiquitous fields. Especially, RTLS(Real-Time Locating Services) dealing with spatio-temporal data is emerging as a promising technology. For these reasons, the ISO/IEC published the RTLS standard specification for compatibility and interoperability in RTLS. Therefore, in this paper, we designed and implemented Spatio-temporal Query Processing Systems for efficiently managing and searching the incoming Spatio-temporal data stream of moving objects. Spatio-temporal Query Processing Systems's spatio-temporal middleware maintains interoperability among heterogeneous devices and guarantees data integrity in query processing through real time processing of unceasing spatio-temporal data streams and two way synchronization of spatio-temporal DBMSs. Web Server uses the SOAP(Simple Object Access Protocol) message between client and server for interoperability and translates client's SOAP message into CQL(Continuous Query Language) of the spatio-temporal middleware. Finally, this thesis proved the utility of the system by applying the spatio-temporal Query Processing Systems to a real-time Locating Services.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.49 no.1
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• pp.81-87
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• 2012

The current defacing method for keeping an anonymity of brain images damages the integrity of a precise brain analysis due to over removal, although it maintains the patients' privacy. A novel method has been developed to create an anonymous face model while keeping the voxel values of an image exactly the same as that of the original one. The method contains two steps: construction of a mockup brain template from ten normalized brain images and a substitution of the mockup brain to the brain image. A level set segmentation algorithm is applied to segment a scalp-skull apart from the whole brain volume. The segmented mockup brain is coregistered and normalized to the subject brain image to create an anonymous face model. The validity of this modification is tested through comparing the intensity of voxels inside a brain area from the mockup brain with the original brain image. The result shows that the intensity of voxels inside from the mockup brain is same as ones from an original brain image, while its anonymity is guaranteed.

• JSTS:Journal of Semiconductor Technology and Science
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• v.14 no.4
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• pp.495-502
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• 2014

Fast recovery diodes (FRDs) were developed using the $p^{{+}{+}}/n^-/n^{{+}{+}}$ epitaxial layers grown by low temperature epitaxy technology. We investigated the effect of electrostatic discharge (ESD) stresses on their electrical and switching properties using current-voltage (I-V) and reverse recovery time analyses. The FRDs presented a high breakdown voltage, >450 V, and a low reverse leakage current, < $10^{-9}$ A. From the temperature dependence of thermal activation energy, the reverse leakage current was dominated by thermal generation-recombination and diffusion, respectively, at low and high temperature regions. By virtue of the abrupt junction and the Pt drive-in for the controlling of carrier lifetime, the soft reverse recovery behavior could be obtained along with a well-controlled reverse recovery time of 21.12 ns. The FRDs exhibited excellent ESD robustness with negligible degradations in the I-V and the reverse recovery characteristics up to ${\pm}5.5$ kV of HBM and ${\pm}3.5$ kV of IEC61000-4-2 shocks. Likewise, transmission line pulse (TLP) analysis reveals that the FRDs can handle the maximum peak pulse current, $I_{pp,max}$, up to 30 A in the forward mode and down to - 24 A in the reverse mode. The robust ESD property can improve the long term reliability of various power applications such as automobile and switching mode power supply.

• The KIPS Transactions:PartD
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• v.8D no.5
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• pp.473-482
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• 2001

Integrated heterogeneous database systems provide the unified interface for users and applications today in order to access the underlying diverse data sources located in different sites. The multiple heterogeneous data sources have the different and specialized data structures and transaction processing capabilities. Because of local autonomy, the local system does not have the capability of cooperation to control the global transaction. Hence designing the global transaction manager with supporting the global serializability is difficult task. To resolve the well-known indirect conflict, we define the global transaction model by using the characteristics of global integrity constraints. And then we propose the site-locking operation and its protocol to manage the global transaction. The correctness and analysis of our site-locking protocol is proved and performance gain over the related other methods is also estimated in this paper.

• KSCI Review
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• v.15 no.1
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• pp.209-217
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• 2007

Korea's seas have the potentials of dispute against China or Japan due to the overlap of the territorial waters and EEZ. In case of marine accidents, the nature of the sea tends to eliminate much of the track, making it another hardship in evidence adoption in case of an international dispute along with the false entries of fishing vessels' journals. Marine Digital Forensics protects the functions of computers and IT appliance on vessels and extracts evidence of voyage and accidents to resolve international dispute. The digital evidence, if tampered with its integrity, may lead to the rejection to a critical claim or may even fail to make a case. As a solution, this thesis suggests Marine Digital Forensics as a way to extract evidence and prove a claim. This may be utilized as means of scientific investigation on sea as overseas exchange increases and the vessels digitalize, leading to a solution in international disputes that may occur in the future.

• Nuclear Engineering and Technology
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• v.26 no.2
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• pp.168-178
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• 1994

Accident Management involves all measures to prevent core damage and retain the core within the reactor vessel, maintain containment integrity and minimize off-site releases. The accident management approach includes : (1) advanced evaluation of candidate strategies, (2) development of procedures to execute appropriate actions efficiently, and (3) identification and provision for materials, tools, and possible modifications to the plant system that may be needed for such execution. When assessing accident management strategies it effectiveness, adverse effect and its feasibility, including information needs and compatibility with existing procedures, must be considered. The objective of this paper is to introduce analytical tools of decision trees and influence diagrams to develop a framework for modeling and assessing severe accident management strategies. The characteristics associated with these took are presented. Based on decision trees and influence diagrams, the framework is applied to a simple example associated with a single decision.

• The KIPS Transactions:PartC
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• v.16C no.2
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• pp.257-266
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• 2009

Now a days, as increased the use of mobile units like a laptop computer and PDA, the demand for high speed internet service is increasing. On the other hand, PKMv2 which is provided from IEEE 802.16e cannot support fully on the security of high speed internet service. This paper proposes light-weight mutual authentication protocol which solved security problem of PKMv2 related to integrity of mobile node for transmission of safe high speed data of mobile node operating in mobile WiMAX environment. Proposed mutual authentication protocol increases the efficiency as the user in network can move in network safely without additional procedure of authentication between subscriber and base station after user's initial authentication. Also, the proposed mutual authentication protocol is safe from the security attack (the man-in-the-middle attack and reply attack) between subscriber and base station by generating a key adopt to PRF() function using random number and secret value in order to secure certification.

• KIPS Transactions on Computer and Communication Systems
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• v.7 no.9
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• pp.235-242
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• 2018

Various technologies have been developed to more efficiently share content such as P2P, CDN, and CCN. These technologies take a common approach that content request packets is responded by distributed network nodes or hosts, not by a single content distributor. Such approaches not only resolve network congestion around content distributors, but also make it possible to distribute content regardless of the system and network status of content distributors. However, when receiving content from distributed nodes/hosts, not from authenticated distributors, users cannot practically identify which node/host sent content to them. Due to this characteristic, various hacking caused by the malicious modification of content is possible. Therefore, to make such approaches more secure, a content authentication technique is required. In this paper, we propose a improved operation of MHT used in CCN for authenticating distributed content. Then we evaluate the proposed method by comparing its performance with the existing technology.