• 한국안전학회지
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• 제21권3호
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• pp.87-93
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• 2006

When a structure is constructed at the site composed of soil, the behavior of a structure is much affected by the characteristics of soil. Therefore, the effect of soil-structure interaction is an important consideration in the design of a structure at the site composed of soil. Precise analysis of soil-structure interaction requires a proper description of soil profile. However, most of approaches are nearly unpractical for soil exhibiting material discontinuity and complex geometry since those cannot consider precisely complicated soil profiles. To overcome these difficulties, an improved integration method is adopted and enables to integrate easily over an element with material discontinuity. As a result the mesh can be generated rapidly and highly structured, leading to regular and precise stiffness matrix. The influence of soil profile on the response is examined by the presented method. It is seen that the presented method can be easily used on soil-structure interaction problems with complicated soil profile and produce reliable results regardless of material discontinuities.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
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• pp.250-256
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• 2004

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.535-538
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• 2000

Streptomyces lavendulae FRI-5 produces the ${\gamma}$-butyrolactone autoregulator IM-2, which is required for nucleoside antibiotic producetion. We have developed a system for introducing DNA into S. lavendule FRI-5 via conjugal transfer from Esherichia cole. Conditions were established for conjugation of the oriT-and attP-containing plasmid pSET152 from E. coli ET12567 (pUZ8002) to FRI-5. Conjugation resulted in integration of the plasmid at the chromosomal C31 attB site. The frequency of intergeneric conjugation varied with the medium used. The highest frequency ($1.6\times10-5$ per recipient) was obtained on ISP medium 2 containing 10mM MgCl2. Southern blot and phenotypic analyses of exconjugants revealed that S. lavendulae FRI-5 contains a unique C31 attB site, and that integration of heterologous DNA into the attB site did not interfere with morphological differentiation or IM-2-dependent signal transduction, including the production of a blue pigment. This system will now enable detailed genetic analysis of the regulation of antibiotic production in S. lavendulae FRI-5.

• 대한원격탐사학회지
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• 제10권2호
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• pp.49-62
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• 1994

Look direction bias in a single look SAR image can often be misinterpreted in the geological application of radar data. This paper investigates digital processing techniques for SAR image data integration and compensation of the SAR data look direction bias. The two important approaches for reducing look direction bias and integration of multiple SAR data sets are (1) principal component analysis (PCA), and (2) wavelet transform(WT) integration techniques. These two methods were investigated and tested with the ERS-1 (VV-polarization) and CCRS*s airborne (HH-polarization) C-SAR image data sets recorded over the Sudbury test site, Canada. The PCA technique has been very effective for integration of more than two layers of digital image data. When there only two sets of SAR data are available, the PCA thchnique requires at least one more set of auxiliary data for proper rendition of the fine surface features. The WT processing approach of SAR data integration utilizes the property which decomposes images into approximated image ( low frequencies) characterizing the spatially large and relatively distinct structures, and detailed image (high frequencies) in which the information on detailed fine structures are preserved. The test results with the ERS-1and CCRS*s C-SAR data indicate that the new WT approach is more efficient and robust in enhancibng the fine details of the multiple SAR images than the PCA approach.

• 미생물학회지
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• 제41권2호
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• pp.140-145
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• 2005

상업적으로 중요한 macrolide계 항진균 학생물질인 natamycin을 생산하는 Streptomyces natalensis ATCC27448의 환자 유전학적인 연구를 위해 대장균으로부터 S. natalensis로 plasmid DNA를 직접 도입하는 형질전환법을 확립하였다. 이러한 S. natalensis의 형질전환은 oriT와 attP 단편을 가지고 있는, ${\Phi}C31$ 유래의 integration 벡터인 pSET152를 이용하여 Escherichia coli ET12567/pUZ28002을 DNA 공여체(donor)로 이용한 접합전달법(conjugal transfer)을 사용하여 확립하였다. 접합전달의 가장 높은 효율은 10 mM의 $MgCl_2$를 포함한 MS 배지에서, $6.25\times10^8$의 E. coli 공여체와 열처리를 하지 않은 S. natalensis의 포자를 사용하여 얻어졌다. 또 얻어진 접합전달체 (exconjugant)에 대하여 southern blot hybridization과 벡터가 삽입된 염색체부분의 염기서열분석을 통해 attB site와 pseudo-attB site를 확인하다. attB site의 경우에는 다른 방선균들처럼 S. natalensis 염색체의 pirin 상동체를 코드하는 ORF내에 존재하였으나 pseudo-attB site는 염색체내 다른 site (GenBank accession no. $YP\_117731$)에 존재하였고 그 염기서열은 attB 염기서열과 차이를 나타내었다.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1221-1227
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• 2008

The application of the cellulase gene (celA) as a selection marker of food-grade integration system was investigated in Lactobacillus (Lb.) casei, Lactococcus lactis, and Leuconostoc (Leu.) mesenteroides. The 6.0-kb vector pOC13 containing celA from Clostridium thermocellum with an integrase gene and a phage attachment site originating from bacteriophage A2 was used for site-specific recombination into chromosomal DNA of lactic acid bacteria (LAB). pOC13 was also equipped with a broad host range plus replication origin from the lactococcal plasmid pWV01, and a controllable promoter of nisA ($P_{nisA}$) for the production of foreign proteins. pOC13 was integrated successfully into Lb. casei EM116, and pOC13 integrants were easily detectable by the formation of halo zone on plates containing cellulose. Recombinant Lb. casei EM 116::pOC13 maintained these traits in the absence of selection pressure during 100 generations. pOC13 was integrated into the chromosome of L. lactis and Leu. mesenteroides, and celA acted as an efficient selection marker. These results show that celA can be used as a food-grade selection marker, and that the new integrative vector could be used for the production of foreign proteins in LAB.

• Journal of Positioning, Navigation, and Timing
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• 제12권1호
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• pp.83-89
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• 2023

The Korea Augmentation Satellite System (KASS) system is a Satellite Based Augmentation System (SBAS) under development to provide APV-I SBAS service in the Republic of Korea. The KASS ground segment generates correction and integrity information for GPS measurements of KASS users using the accurate positions of KASS Reference Station (KRS) antenna phase centers. For this reason, the accuracy of KRS reference points through geodetic survey campaigns is one of the important factors for providing the KASS service in compliance with the required navigation performance. In order to obtain accurate positions, two geodetic survey campaigns were performed at several reference points, such as Mark, Center of Mast at Ground Level (CMGL), and Center of Hole in Top Plate (CHTP), of each KRS site using three different survey methods, the Virtual Reference Station (VRS), Flächen Korrektur Parameter (FKP), and raw data post-processing methods. By comparing and analyzing the results, the computed coordinates of the reference points were verified and Antenna Phase Center (APC) positions were calculated using KRS Antenna Reference Point (ARP) data, and the first KASS Site Acceptance Test (SAT#1) was performed successfully using the verified APC coordinates. After the first site survey activities, the KASS operators should maintain the coordinates with the required performance such that the overall KASS navigation performance commitment is guaranteed during the lifetime of 15 years. Therefore, the maintenance plan for the KRS antenna coordinates should be developed before the commissioning of KASS operation planned after 2023. Therefore, this paper presents a geodetic survey method selected for the maintenance activities and provides the rationale for using this method.

• 한국BIM학회 논문집
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• 제5권4호
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• pp.47-52
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• 2015

This paper presents a case study using Building Information Modeling (BIM) for planning, simulating, and implementing complex site logistics in a headquarter office building construction project in Silver Spring, MD. As part of the project a prefabricated 92ft structural tube steel pedestrian connector bridge was installed between two adjacent buildings in the city of Silver Spring, MD. There were multiple significant challenges to deliver, offload, prepare, and install the connector bridge safely, on time, and with the minimum disturbances to the neighbors. BIM was of the foremost importance to visualize, simulate, analyze, improve, and communicate the site logistics plan from delivery to installation of the connector bridge. As a result of the effort, GC of the project was able to prepare a highly detailed plan, communicate it effectively to all stakeholders, and flawlessly execute the work as planned. This case study would provide a useful reference for contractors who are seeking a better planning method that enables generation of more accurate, implementable, optimized plans for complex site logistics.

• KSBB Journal
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• 제25권2호
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• pp.167-172
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• 2010

농작물에 심각한 피해를 주는 phytotoxin을 생산하는 S. scabiei ATCC 49173의 분자 유전학적인 연구를 위해 대장균으로부터 S. scabiei로 plasmid DNA를 도입하는 접합 전달법을 이용한 형질전환법을 확립하였다. 본 연구를 통해 확인된 S. scabiei의 접합전달용 최적배지는 50 mM의 $MgCl_2$를 첨가한 MS배지이며 접합전달에 사용되는 DNA 수용체인 포자는 $45^{\circ}C$의 열처리와 $5{\times}10^7$이상의 plasmid DNA 공여체가 필요하다는 것을 확인하였다. 또 얻어진 접합전달체에 대하여 Southern blot hybridization과 벡터가 삽입된 염색체부분의 염기서열분석을 통해 attB site의 특성을 분석한 결과 S. scabiei 염색체의 pirin 상동체를 코드하는 ORF내에 단일위치로 존재하고 있으며 이미 밝혀진 다른 방선균유래 attB site의 염기서열에 대해 86.3%~96.1%의 상동성을 보였다.

• Smart Structures and Systems
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• 제15권4호
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• pp.1019-1039
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• 2015

Shanghai Tower is a composite structure building with a height of 632 m. In order to verify the structural properties and behaviors in construction and operation, a structural health monitoring project was conducted by Tongji University. The monitoring system includes sensor system, data acquisition system and a monitoring software system. Focusing on the health monitoring in construction, this paper introduced the monitoring parameters in construction, the data acquisition strategy and an integration structural health monitoring (SHM) software. The integration software - Structural Monitoring/ Analysis/ Evaluation System (SMAE) is designed based on integration and modular design idea, which includes on-line data acquisition, finite elements and dynamic property analysis functions. With the integration and modular design idea, this SHM system can realize the data exchange and results comparison from on-site monitoring and FEM effectively. The analysis of the monitoring data collected during the process of construction shows that the system works stably, realize data acquirement and analysis effectively, and also provides measured basis for understanding the structural state of the construction. Meanwhile, references are provided for the future automates construction monitoring and implementation of high-rise building structures.