• Journal of the Korean Society of Safety
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• v.21 no.3 s.75
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• pp.87-93
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• 2006

When a structure is constructed at the site composed of soil, the behavior of a structure is much affected by the characteristics of soil. Therefore, the effect of soil-structure interaction is an important consideration in the design of a structure at the site composed of soil. Precise analysis of soil-structure interaction requires a proper description of soil profile. However, most of approaches are nearly unpractical for soil exhibiting material discontinuity and complex geometry since those cannot consider precisely complicated soil profiles. To overcome these difficulties, an improved integration method is adopted and enables to integrate easily over an element with material discontinuity. As a result the mesh can be generated rapidly and highly structured, leading to regular and precise stiffness matrix. The influence of soil profile on the response is examined by the presented method. It is seen that the presented method can be easily used on soil-structure interaction problems with complicated soil profile and produce reliable results regardless of material discontinuities.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2004.06a
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• pp.250-256
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• 2004

• Journal of Microbiology and Biotechnology
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• v.10 no.4
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• pp.535-538
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• 2000

Streptomyces lavendulae FRI-5 produces the ${\gamma}$-butyrolactone autoregulator IM-2, which is required for nucleoside antibiotic producetion. We have developed a system for introducing DNA into S. lavendule FRI-5 via conjugal transfer from Esherichia cole. Conditions were established for conjugation of the oriT-and attP-containing plasmid pSET152 from E. coli ET12567 (pUZ8002) to FRI-5. Conjugation resulted in integration of the plasmid at the chromosomal C31 attB site. The frequency of intergeneric conjugation varied with the medium used. The highest frequency ($1.6\times10-5$ per recipient) was obtained on ISP medium 2 containing 10mM MgCl2. Southern blot and phenotypic analyses of exconjugants revealed that S. lavendulae FRI-5 contains a unique C31 attB site, and that integration of heterologous DNA into the attB site did not interfere with morphological differentiation or IM-2-dependent signal transduction, including the production of a blue pigment. This system will now enable detailed genetic analysis of the regulation of antibiotic production in S. lavendulae FRI-5.

• Korean Journal of Remote Sensing
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• v.10 no.2
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• pp.49-62
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• 1994

Look direction bias in a single look SAR image can often be misinterpreted in the geological application of radar data. This paper investigates digital processing techniques for SAR image data integration and compensation of the SAR data look direction bias. The two important approaches for reducing look direction bias and integration of multiple SAR data sets are (1) principal component analysis (PCA), and (2) wavelet transform(WT) integration techniques. These two methods were investigated and tested with the ERS-1 (VV-polarization) and CCRS*s airborne (HH-polarization) C-SAR image data sets recorded over the Sudbury test site, Canada. The PCA technique has been very effective for integration of more than two layers of digital image data. When there only two sets of SAR data are available, the PCA thchnique requires at least one more set of auxiliary data for proper rendition of the fine surface features. The WT processing approach of SAR data integration utilizes the property which decomposes images into approximated image ( low frequencies) characterizing the spatially large and relatively distinct structures, and detailed image (high frequencies) in which the information on detailed fine structures are preserved. The test results with the ERS-1and CCRS*s C-SAR data indicate that the new WT approach is more efficient and robust in enhancibng the fine details of the multiple SAR images than the PCA approach.

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.140-145
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• 2005

Streptomyces natalensis ATCC27448 produces natamycin, a commercially important macrolide antifungal antibiotic. For molecular genetic study of S. natalensis, we have developed a system for introducing DNA into S. natalensis via conjugal transfer from Escherichia coli. An effective transformation procedure for S. natalensis was established based on transconjugation from E, coli ET12567/pUZ8002 using a ${\Phi}C31$-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 10 mM $MgCl_2$ using $6.25\times10^8$ of E.coli donor cells without heat treatment of spores. In addition, southern blot analysis of exconjugants and the sequence of plasmids containing DNA flanking the insertion sites from the chromosome revealed that S. natalensis contains a single ${\Phi}C31$ attB site and at least a secondary or pseudo attB site. Similar to the case of various Streptomyces species, a single ${\Phi}C31$ attB site of S. natalensis is present within an ORF encoding a pirin-homolog, but a pseudo-attB site is present within a distinct site (GenBank accession no. $YP\_117731$) and also its sequence deviates from the consensus sequences of attB sequence.

• Food Science and Biotechnology
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• v.17 no.6
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• pp.1221-1227
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• 2008

The application of the cellulase gene (celA) as a selection marker of food-grade integration system was investigated in Lactobacillus (Lb.) casei, Lactococcus lactis, and Leuconostoc (Leu.) mesenteroides. The 6.0-kb vector pOC13 containing celA from Clostridium thermocellum with an integrase gene and a phage attachment site originating from bacteriophage A2 was used for site-specific recombination into chromosomal DNA of lactic acid bacteria (LAB). pOC13 was also equipped with a broad host range plus replication origin from the lactococcal plasmid pWV01, and a controllable promoter of nisA ($P_{nisA}$) for the production of foreign proteins. pOC13 was integrated successfully into Lb. casei EM116, and pOC13 integrants were easily detectable by the formation of halo zone on plates containing cellulose. Recombinant Lb. casei EM 116::pOC13 maintained these traits in the absence of selection pressure during 100 generations. pOC13 was integrated into the chromosome of L. lactis and Leu. mesenteroides, and celA acted as an efficient selection marker. These results show that celA can be used as a food-grade selection marker, and that the new integrative vector could be used for the production of foreign proteins in LAB.

• Journal of Positioning, Navigation, and Timing
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• v.12 no.1
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• pp.83-89
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• 2023

The Korea Augmentation Satellite System (KASS) system is a Satellite Based Augmentation System (SBAS) under development to provide APV-I SBAS service in the Republic of Korea. The KASS ground segment generates correction and integrity information for GPS measurements of KASS users using the accurate positions of KASS Reference Station (KRS) antenna phase centers. For this reason, the accuracy of KRS reference points through geodetic survey campaigns is one of the important factors for providing the KASS service in compliance with the required navigation performance. In order to obtain accurate positions, two geodetic survey campaigns were performed at several reference points, such as Mark, Center of Mast at Ground Level (CMGL), and Center of Hole in Top Plate (CHTP), of each KRS site using three different survey methods, the Virtual Reference Station (VRS), Flächen Korrektur Parameter (FKP), and raw data post-processing methods. By comparing and analyzing the results, the computed coordinates of the reference points were verified and Antenna Phase Center (APC) positions were calculated using KRS Antenna Reference Point (ARP) data, and the first KASS Site Acceptance Test (SAT#1) was performed successfully using the verified APC coordinates. After the first site survey activities, the KASS operators should maintain the coordinates with the required performance such that the overall KASS navigation performance commitment is guaranteed during the lifetime of 15 years. Therefore, the maintenance plan for the KRS antenna coordinates should be developed before the commissioning of KASS operation planned after 2023. Therefore, this paper presents a geodetic survey method selected for the maintenance activities and provides the rationale for using this method.

• Journal of KIBIM
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• v.5 no.4
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• pp.47-52
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• 2015

This paper presents a case study using Building Information Modeling (BIM) for planning, simulating, and implementing complex site logistics in a headquarter office building construction project in Silver Spring, MD. As part of the project a prefabricated 92ft structural tube steel pedestrian connector bridge was installed between two adjacent buildings in the city of Silver Spring, MD. There were multiple significant challenges to deliver, offload, prepare, and install the connector bridge safely, on time, and with the minimum disturbances to the neighbors. BIM was of the foremost importance to visualize, simulate, analyze, improve, and communicate the site logistics plan from delivery to installation of the connector bridge. As a result of the effort, GC of the project was able to prepare a highly detailed plan, communicate it effectively to all stakeholders, and flawlessly execute the work as planned. This case study would provide a useful reference for contractors who are seeking a better planning method that enables generation of more accurate, implementable, optimized plans for complex site logistics.

• KSBB Journal
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• v.25 no.2
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• pp.167-172
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• 2010

Streptomyces scabiei producing phytotoxin called thaxtomin, which cause scab disease on economically important crops such as potato. For molecular genetics study of S. scabiei an effective transformation method was established based on conjugal transfer from Escherichia coli ET12567 (pUZ8002) using a phiC31-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 50 mM $MgCl_2$. In addition, the sequence and location of the chromosomal integration attB site of S. scabiei was identified for the first time in the strains producing thaxtomin by the southern blot analysis of exconjugants and the sequencing of plasmid containing DNA flanking the insertion sites from exconjugant chromosome. Similar to the case of Streptomyces species, a single phiC31 attB site of S. scabiei is present within an ORF encoding a pirin-homolog.

• Smart Structures and Systems
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• v.15 no.4
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• pp.1019-1039
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• 2015

Shanghai Tower is a composite structure building with a height of 632 m. In order to verify the structural properties and behaviors in construction and operation, a structural health monitoring project was conducted by Tongji University. The monitoring system includes sensor system, data acquisition system and a monitoring software system. Focusing on the health monitoring in construction, this paper introduced the monitoring parameters in construction, the data acquisition strategy and an integration structural health monitoring (SHM) software. The integration software - Structural Monitoring/ Analysis/ Evaluation System (SMAE) is designed based on integration and modular design idea, which includes on-line data acquisition, finite elements and dynamic property analysis functions. With the integration and modular design idea, this SHM system can realize the data exchange and results comparison from on-site monitoring and FEM effectively. The analysis of the monitoring data collected during the process of construction shows that the system works stably, realize data acquirement and analysis effectively, and also provides measured basis for understanding the structural state of the construction. Meanwhile, references are provided for the future automates construction monitoring and implementation of high-rise building structures.