Browse > Article

Construction of Transformation Method for Streptomyces scabiei ATCC 49173 Producing Phytotoxin  

Jang, Bo-Youn (Department of Food Science and Biotechnology, Kyungnam University)
Ha, Heon-Su (Department of Food Science and Biotechnology, Kyungnam University)
Choi, Sun-Uk (Department of Food Science and Biotechnology, Kyungnam University)
Publication Information
KSBB Journal / v.25, no.2, 2010 , pp. 167-172 More about this Journal
Abstract
Streptomyces scabiei producing phytotoxin called thaxtomin, which cause scab disease on economically important crops such as potato. For molecular genetics study of S. scabiei an effective transformation method was established based on conjugal transfer from Escherichia coli ET12567 (pUZ8002) using a phiC31-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 50 mM $MgCl_2$. In addition, the sequence and location of the chromosomal integration attB site of S. scabiei was identified for the first time in the strains producing thaxtomin by the southern blot analysis of exconjugants and the sequencing of plasmid containing DNA flanking the insertion sites from exconjugant chromosome. Similar to the case of Streptomyces species, a single phiC31 attB site of S. scabiei is present within an ORF encoding a pirin-homolog.
Keywords
Streptomyces scabiei ATCC 49173; transformation; attB site; phytotoxin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Paranthaman, S. and K. Dharmalingam (2003) Intergeneric conjugation in Streptomyces peucetius and Streptomyces sp. strain C5: Chromosomal integration and expression of recombinant plasmids carrying the chiC gene. Appl. Environ. Microbiol. 69: 84-91.   DOI   ScienceOn
2 Matsushima, P., M. C. Broughton, J. R. Turner, and R. H. Baltz (1994) Conjugal transfer of cosmid DNA from Escherichia coli to Saccharopolyspora spinosa: effects of chromosomal insertions on macrolide A83543 production. Gene 146: 39-45.   DOI   ScienceOn
3 Choi, S. U., C. K. Lee, Y. I. Hwang, H. Kinoshita, and T. Nihira (2004) Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Kitasatospora setae, a bafilomycin B1 producer. Arch. Microbiol. 181: 294-298.   DOI   ScienceOn
4 Engel, P. (1987) Plasmid transformation of Streptomyces tendae after heat attenuation of restriction. Appl Environ Microbiol. 53: 1-3.
5 Bailey, C. R. and D. J. Winstanley (1986) Inhibition of restriction in Streptomyces clavuligerus by heat treatment. J. Gen. Microbiol. 132: 2945-2947.
6 Smokvina, T., P. Mazodier, F. Boccard, C. J. Thompson, and M. Guerineau (1990) Construction of a series of pSAM2-based integrative vectors for use in actinomycetes. Gene 94: 53-59.   DOI   ScienceOn
7 Kieser, T., M. J. Bibb, M. J. Buttner, K. F. Chater, and D. A. Hopwood (2000) Practical Streptomyces Genetics. The John Innes Foundation, Norwich, U.K.
8 Rao, R. N., M. A. Richardson, and S. Kuhstoss (1987) Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA. Methods Enzymol. 153: 166-198.   DOI
9 Bierman, M., R. Logan, K. O'Brien, E. T. Seno, R. N. Rao, and B. E. Schoner (1992) Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116: 43-49.   DOI   ScienceOn
10 Motamedi, H., A. Shafiee, and S. J. Cai (1995) Integrative vectors for heterologous gene expression in Streptomyces spp. Gene 160: 25-31.   DOI   ScienceOn
11 King, R. R., C. H. Lawrence, and M. C. Clark (1991) Correlation of phytotoxin production with pathogenicity of Streptomyces scabies isolates from scab infected tubers. J. Am. Potato Assoc. 68: 675-680.   DOI   ScienceOn
12 Stegmann, E., S. Pelzer, K. Wilken, and W. Wohlleben (2001) Development of three different gene cloning systems for genetic investigation of the new species Amycolatopsis japonicum MG417-CF17, the ethylenediaminedisuccinic acid producer. J. Biotechnol. 92: 195-204.   DOI   ScienceOn
13 Sambrook, J. and D. W. Russell (2001) Molecular Cloning: A Laboratory Manual. 3rd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
14 Kitani, S., M. J. Bibb, T. Nihira, and Y. Yamada (2000) Conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces lavendulae FRI-5. J. Microbiol. Biotechnol. 10: 535-538.
15 Mazodier, P., R. Petter, and C. Thompson (1989) Intergeneric conjugation between Escherichia coli and Streptomyces species. J. Bacteriol. 171: 3583-3585.   DOI
16 King, R. R., C. H. Lawrence, and L. A. Calhoun (1992) Chemistry of phytotoxins associated with Streptomyces scabies, the causal organism of potato common scab. J. Agric. Food Chem. 40: 834-837.   DOI
17 Baltz, R. H. (1998) Genetic manipulation of antibioticproducing Streptomyces. Trends Microbiol. 6: 76-82.   DOI   ScienceOn
18 MacNeil, D. J. (1988) Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J. Bacteriol. 170: 5607-5612.   DOI
19 Flett, F., V. Mersinias, and C. P. Smith (1997) High efficiency intergeneric conjugal transfer of plasmid DNA from Escherichia coli to methyl DNA-restricting streptomycetes. FEMS Microbiol. Lett. 155: 223-229.   DOI   ScienceOn