• Title/Summary/Keyword: Integration method

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Improved plastid transformation efficiency in Scoparia dulcis L.

  • Kota, Srinivas;Hao, Qiang;Narra, Muralikrishna;Anumula, Vaishnavi;Rao, A.V;Hu, Zanmin;Abbagani, Sadanandam
    • Journal of Plant Biotechnology
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    • v.46 no.4
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    • pp.323-330
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    • 2019
  • The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum-specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the transformation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.

Undamped Dynamic Response of Anisotropic Laminated Composite Plates and Shell Structures using a Higher-order Shear Deformation Theory (비등방성 복합적층판 및 쉘의 고차전단변형을 고려한 비감쇄 동적응답)

  • Yoon, Seok Ho;Han, Seong Cheon;Chang, Suk Yoon
    • Journal of Korean Society of Steel Construction
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    • v.9 no.3 s.32
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    • pp.333-340
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    • 1997
  • This paper will expand the third-order shear deformation theory by the double-Fourier series and reduce to the solution of a system of ordinary differential equations in time, which are integrated numerically using Newmark's direct integration method and clarify the undamped dynamic responses for the cross-ply and antisymmetric angle-ply laminated composite plates and shells with simply supported boundary condition. Numerical results for deflections are presented showing the effect of side-to-thickness ratio, aspect ratio, material anisotropy, and lamination scheme.

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Undergraduate Nursing Students' Perceived Knowledge and Attitudes Toward Genetics and Nursing Competencies for Genetics (간호대학생이 인지하는 유전학에 대한 지식과 태도 및 유전간호 역량)

  • Choi, Hyunkyung
    • Journal of Korean Biological Nursing Science
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    • v.16 no.2
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    • pp.69-79
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    • 2014
  • Purpose: The purpose of this descriptive, cross-sectional study was to examine Korean undergraduate nursing students' perceived knowledge and attitudes toward genetics. Nursing competencies for genetics among nursing students were also assessed. Methods: Two hundred eighty-six Korean junior and senior undergraduate nursing students who went through their clinical practicum completed a packet of self-report questionnaires exploring opinions about the integration of genetics into the nursing curriculum, as well as their perceived knowledge, attitudes, and nursing competencies toward genetics. Collected data were analyzed using descriptive statistics, t-test, one-way ANOVA. Results: Overall, Korean undergraduate nursing students reported limited genetic knowledge, but most of them were comfortable with drawing pedigree. In general, nursing students were competent in providing genetic nursing. Their genetic nursing competencies were significantly different depending on the level of general understanding of genetics. There was no significant difference between juniors and seniors regarding knowledge, attitudes, and competencies toward genetics. More than 60% of nursing students reported the need for genetic nursing, and the majority considered lectures to be the most effective method to learn genetic knowledge. Conclusion: It is imperative to integrate genetics into nursing curriculum in order to improve nursing students' genetic nursing competencies as well as genetic knowledge.

A Study on the Formal Generation Characteristics in the Digital Design Process (디지털 디자인프로세스에 나타난 형태생성방식의 특성에 관한 연구)

  • Lee, Su-Yong;Lee, Sang-Joon
    • Journal of The Korean Digital Architecture Interior Association
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    • v.6 no.2
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    • pp.9-16
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    • 2006
  • The research want to know about form creation method's special quality that found in digital construction design process that using digital media, and they comparison analysis that who positive using digital media, Frank O. Gehry, Peter Eisenman, Greg Lynn, UN studio's work. First, In digital design process using digital media is make full use to design unfolding step and application way can change by architect's recognition difference of using digital media and each architect's design general idea unfolding process, and it means form creation process can change. Second, In digital design process a building form doesn't design adaptation to construction system, they directly design the third dimension form. So, they deduction a new form that unification and integration. Third, the architect that appear's in digital design process's part is existing, data from position of the master which it selects the coding selects the outcome goods which appears in computer operation with coordinator role of the originality and construction sense which it is visible.

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Prediction of Etch Profile Uniformity Using Wavelet and Neural Network

  • Park, Won-Sun;Lim, Myo-Taeg;Kim, Byungwhan
    • International Journal of Control, Automation, and Systems
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    • v.2 no.2
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    • pp.256-262
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    • 2004
  • Conventionally, profile non-uniformity has been characterized by relying on approximated profile with angle or anisotropy. In this study, a new non-uniformity model for etch profile is presented by applying a discrete wavelet to the image obtained from a scanning electron microscopy (SEM). Prediction models for wavelet-transformed data are then constructed using a back-propagation neural network. The proposed method was applied to the data collected from the etching of tungsten material. Additionally, 7 experiments were conducted to obtain test data. Model performance was evaluated in terms of the average prediction accuracy (APA) and the best prediction accuracy (BPA). To take into account randomness in initial weights, two hundred models were generated for a given set of training factors. Behaviors of the APA and BPA were investigated as a function of training factors, including training tolerance, hidden neuron, initial weight distribution, and two slopes for bipolar sig-moid and linear function. For all variations in training factors, the APA was not consistent with the BPA. The prediction accuracy was optimized using three approaches, the best model based approach, the average model based approach and the combined model based approach. Despite the largest APA of the first approach, its BPA was smallest compared to the other two approaches.

Design and Construction of Analysis System using R (R 을 이용한 분석 시스템 구축 방안 및 설계)

  • Kwon, Youngoh;Jin, Byoungsam;Bae, Youngchul
    • Journal of Platform Technology
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    • v.6 no.1
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    • pp.9-16
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    • 2018
  • In recent years, many analysts have been using Python and R to analyze data using efficient analysis and statistics. However, it is difficult to solve the requirement to express various analyzes in real time through the web system using only two languages. In addition, it is possible to confirm that the extracted P-Value is different from the value in R because there is no corresponding algorithm in Java or the corresponding source is not verified. Therefore, in this paper, we propose a method to apply analyst 's analysis algorithm contents directly to his system using R in other languages.

Demand and Requirement Analysis on the Flexible Method of Long-life Housing (장수명주택 가변 방식에 대한 수요 및 요구사항 분석)

  • Park, Ji-Young;Song, Sang-Hoon;Park, Hyo-Eun
    • Journal of the Korean housing association
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    • v.27 no.3
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    • pp.1-9
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    • 2016
  • It is essential to maximize the advantages of long-life housing in consideration of the occupants' demands in order to promote the construction of long-life housing. The diverse plan types with flexibility should be developed based on those demands. Therefore, this study aims to review the level of awareness of long-life housing, and identify the customer needs by conducting the survey for the preference to pre-defined plan types. The results of survey are as follows: 1) Despite the enforcement of the Long-life Housing Certification System in 2014, the recognition among the customers about the long-life housing is still on the low level as shown in that 79.9% of respondents were not aware of the long-life housing; 2) For three alternative plan types, 78.5% on the integration type in front, 63.1% on the moving kitchen type, 47.0% on the Divided Housing type had intention to change; 3) 68.9% of the total are willingly ready to purchase the long-life housing under the condition of no difference in price between normal apartment and long-life housing. The less the number of family members is, the bigger this ratio of positive purchase intention is. Reflecting these results into the designs is expected to contribute to the enhancement of customer satisfaction as well as the diffusion of long-life housing.

Collaborative Process Modeling for Embodying e-Manufacturing (이메뉴팩처링을 위한 협업 프로세스 모델링)

  • Ryu, Kwang-Yeol;Cho, Yong-Ju;Choi, Hon-Zong;Lee, Seok-Woo
    • IE interfaces
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    • v.18 no.3
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    • pp.221-233
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    • 2005
  • e-Manufacturing can be referred to as a system methodology enabling the integration of manufacturing operations and IT technologies to achieve objectives of an enterprise. It is recently regarded as a powerful solution to survive in a chaotic marketplace. While conducting an e-Manufacturing project, we first needed to capture collaborative processes conducted by multiple participants in order to define functions of a system supporting them. However, pervasive process modeling techniques including IDEF3, Petri nets, and UML are not sufficient for modeling collaborative processes. Therefore, we first briefly investigate several process modeling methods including aforementioned modeling methods and ARIS focusing on the collaboration point of view. Then, we propose a new modeling method referred to as Collaborative Process Modeling (CPM) to clearly describe collaborative processes. Also, we develop and illustrate a rule for transforming collaborative process models into Marked Graph models to use the analysis power of the Petri nets. Using CPM empowers us to develop collaborative process models with simple notations, understand and facilitate the realization of the collaboration, and verify models before rushing into the development.

A System Development of Generating Ubiquitous Service Scenarios and System Analysis (유비쿼터스 서비스 시나리오 생성 및 시스템 분석 시스템 개발)

  • Lee, Yon-Nim;Jung, Dong-Young;Kwon, Oh-Byung
    • Journal of Intelligence and Information Systems
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    • v.14 no.4
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    • pp.161-178
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    • 2008
  • Development and realization of ubiquitous services in physical space is now in progress to develop ubiquitous smart space (USS). As the development methodologies for USS, scenario development is performed before requirement analysis and design, or vice versa. However, even though lots of redundant elements could be found between scenarios and requirement analysis results, a sort of structural approaches to join them together has been still very rare. Hence, the aim of this paper is to propose a system which generates ubiquitous service senarios and system analysis specifications according to a novel integration methodology. To do so, scenario and requirement analysis are integrated in a structured manner.

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The 'Good Nursing' Experienced by Hemodialysis Patients (혈액투석 환자가 경험한 '좋은 간호')

  • Yeom, Eun Yi;Kim, Miyoung
    • Korean Journal of Adult Nursing
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    • v.26 no.3
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    • pp.275-286
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    • 2014
  • Purpose: The purpose of this study was to explore the meaning and essence of good nursing from the perspective of patients undergoing hemodialysis. Methods: The data were collected through in-depth interviews of five hemodialysis patients who have been dialyzed for one year or longer. The data analysis was conducted using the Giorgi's phenomenological method. Results: The findings included six constituents and 13 sub-constituents. The six constituents were 'prompt and skillful response to mitigate distress', 'careful attention to provide relief', 'individualized education to promote self-management', 'an intimate relationship to regain isolated ego', 'warmhearted support to encourage strength to live in a exhausting life', and 'providing of comfortable environment to maintain stability'. Conclusion: From the perspective of the hemodialysis patients, the concept of good nursing should stem from the integration of nurses' scientific knowledge, expert skills, and moral sensitivity. The finding suggests that strategies to develop nursing interventions for hemodialysis patients will be required on the basis of understanding of their sociocultural contexts and familism.