• Journal of agriculture & life science
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• v.46 no.1
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• pp.163-176
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• 2012

TTo increase productivity of a strong extracellular alkaline protease (BCAP), stable strains of Bacillus clausii I-52 carrying another copy of BCAP gene in the chromosome were developed. Integrative vector, pHPS9-fuBCAP carrying BCAP promoter, ribosome binding site, signal sequence and active protease gene was constructed and transferred into B. clausii I-52, and integration of the constructed plasmid into chromosome was identified by PCR. An investigation was carried out on BCAP production by B. clausii I-52 and transformant C5 showing the highest relative activity of alkaline protease using submerged fermentation. Maximum enzyme activity was produced when cells were grown under the submerged fermentation conditions at $37^{\circ}C$ for 48 h with an aeration rate of 1 vvm and agitation rate of 650 rpm in a optimized medium (soybean meal 2%, wheat flour 1%, sodium citrate 0.5%, $K_2HPO_4$ 0.4%, $Na_2HPO_4$ 0.1%, NaCl 0.4%, $MgSO_47H_2O$ 0.01%, $FeSO_47H_2O$ 0.05%, liquid maltose 2.5%, $Na_2CO_3$ 0.6%). A protease yield of approximately 134,670U/ml was achieved using an optimized media, which show an increase of approximately 1.6-fold compared to that of non-transformant (83,960 U/ml). When the stability of transformant C5 was examined, the integrated plasmid pHPS9-fuBCAP was detected in the transformant after cultivation for 8 days, suggesting that it maintained stably in the chromosomal DNA of transformant C5.

• Ecology and Resilient Infrastructure
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• v.8 no.4
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• pp.194-203
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• 2021

With the recent increase in maintenance works on water and sewer pipes as well as district heating supply pipes, pavement cutting work using pavement cutter is on the rise. The pavement cutting operation generates considerable dust (cutting sludge) as well as noise; therefore, it is necessary to apply eco-friendly technologies that have low noise and dust recovery capability. Thus far, various equipment for recovering dust have been developed; however, there is a limitation in that the environmental friendliness is not quantified. Therefore, in this study, we developed a low-noise, dust-recovery type pavement cutter that can fundamentally remove the causes of environmental hazards such as noise and dust and evaluated the eco-friendliness of the pavement cutting process performed by this cutter. To this end, an integrated water cooling-sludge recovery system composed of a vacuum device and a sludge suction unit was developed, and the developed system was applied to a pavement cutter. Subsequently, the developed equipment was applied to the test bed, and data related to its eco-friendliness were collected and evaluated. The results showed that the cutting sludge recovery rate of the developed equipment was greater than 83%, the noise level was approximately 82 - 83 dB, and the sound power level was 115 dB. The results of this study will be used as basic data to develop improved pavement cutters in the future with improved cutting sludge recovery performance and lower noise.

• Journal of Trauma and Injury
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• v.32 no.3
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• pp.143-149
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• 2019

Purpose: No previous study has assessed elderly pedestrian traffic accidents based on a nationwide database. This study aimed to help primary physicians who examine patients in emergency departments to determine and make prompt and accurate treatment decisions. Methods: This study used data from the Emergency Department-based Injury Indepth Surveillance from 2013 to 2017, managed by the Korea Centers for Disease Control and Prevention. Pedestrians aged ${\geq}65years$ were included, and using multivariate logistic regression multiple factors were analyzed to determine their relationship with injury severity. Results: Of 227,695 subjects, 6,498 were included, of whom 2,065 (31.8%) were severely injured. There were more female than male patients in all severity groups. Most accidents occurred in the afternoon and on general roads. In the multivariate analysis, the odds ratio (OR) of injury severity for male pedestrians was 1.165 (95% confidence interval: 1.034-1.313, p=0.012). Older age of patients and the use of ambulances were associated with greater injury severity. The accident time affected the degree of injury severity; i.e., compared to dawn, injury severity increased in the morning (OR: 1.246, p=0.047) and decreased at night (OR: 0.678, p<0.001). A significant difference was noted in the correlation between the type of vehicle causing the accident and the accident severity; i.e., motorcycle accidents had lower severity than bicycle accidents (OR: 0.582, p=0.047). Conclusions: Injury severity was correlated with sex, age, transportation to the ED, TA onset time, and type of vehicle. The study results suggest that injury severity may be positively reflected in initial assessments and overall integrated treatments by physicians and in the related policies.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.38 no.6
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• pp.967-977
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• 2018

The problems of lack of skilled worker and poor productivity at the construction site continue to be raised, and the introduction of construction automation as one of the solutions to this has been considered. The development of various types of equipment and systems has been carried out, especially for earthwork operations with multiple construction equipment. However, the level of commercialization of developed equipment or systems is very limited. Although the single-product type of earthwork equipment has been applied to the site, the integrated type of earthwork system is still in the field testing stage. Considering these constraints, the limited budget and research period, a strategy is needed to identify which technology areas and core technologies should be developed first. In this study, the technology areas and detailed core technologies that are essential for the development of earthwork systems at the level of commercialization are set and the priority of development is determined. In addition, the earthwork system that has been developed so far is analyzed and the detailed development direction is presented based on it. The findings can be used for a decision making to set the priority for core technologies that should be developed first in the limited budget and period.

• Mycobiology
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• v.49 no.4
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• pp.406-420
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• 2021

Gloeostereum incarnatum has edible and medicinal value and was first cultivated and domesticated in China. We sequenced the G. incarnatum monokaryotic strain GiC-126 on an Illumina HiSeq X Ten system and obtained a 34.52-Mb genome assembly sequence that encoded 16,895 predicted genes. We combined the GiC-126 genome with the published genome of G. incarnatum strain CCMJ2665 to construct a genetic linkage map (GiC-126 genome) that had 10 linkage groups (LGs), and the 15 assembly sequences of CCMJ2665 were integrated into 8 LGs. We identified 1912 simple sequence repeat (SSR) loci and detected 700 genes containing 768 SSRs in the genome; 65 and 100 of them were annotated with gene ontology (GO) terms and KEGG pathways, respectively. Carbohydrate-active enzymes (CAZymes) were identified in 20 fungal genomes and annotated; among them, 144 CAZymes were annotated in the GiC-126 genome. The A mating-type locus (MAT-A) of G. incarnatum was located on scaffold885 at 38.9 cM of LG1 and was flanked by two homeodomain (HD1) genes, mip and beta-fg. Fourteen segregation distortion markers were detected in the genetic linkage map, all of which were skewed toward the parent GiC-126. They formed three segregation distortion regions (SDR1-SDR3), and 22 predictive genes were found in scaffold1920 where three segregation distortion markers were located in SDR1. In this study, we corrected and updated the genomic information of G. incarnatum. Our results will provide a theoretical basis for fine gene mapping, functional gene cloning, and genetic breeding the follow-up of G. incarnatum.

• Journal of Industrial Convergence
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• v.20 no.3
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• pp.33-40
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• 2022

Recently, IoT-linked services have been used in various environments, and IoT and artificial intelligence technologies are being fused. However, since technologies that process IoT data stably are not fully supported, research is needed for this. In this paper, we propose a processing technique that can optimize IoT data after generating embedded vectors based on machine learning for IoT data. In the proposed technique, for processing efficiency, embedded vectorization is performed based on QR such as index of IoT data, collection location (binary values of X and Y axis coordinates), group index, type, and type. In addition, data generated by various IoT devices are integrated and managed so that load balancing can be performed in the IoT data collection process to asymmetrically link IoT data. The proposed technique processes IoT data to be orthogonalized based on hash so that IoT data can be asymmetrically grouped. In addition, interference between IoT data may be minimized because it is periodically generated and grouped according to IoT data types and characteristics. Future research plans to compare and evaluate proposed techniques in various environments that provide IoT services.

• Smart Structures and Systems
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• v.29 no.4
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• pp.625-640
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• 2022

Maglev rail joints are vital components serving as connections between the adjacent F-type rail sections in maglev guideway. Damage to maglev rail joints such as bolt looseness may result in rough suspension gap fluctuation, failure of suspension control, and even sudden clash between the electromagnets and F-type rail. The condition monitoring of maglev rail joints is therefore highly desirable to maintain safe operation of maglev. In this connection, an online damage detection approach based on three-dimensional (3D) convolutional neural network (CNN) and time-frequency characterization is developed for simultaneous detection of multiple damage of maglev rail joints in this paper. The training and testing data used for condition evaluation of maglev rail joints consist of two months of acceleration recordings, which were acquired in-situ from different rail joints by an integrated online monitoring system during a maglev train running on a test line. Short-time Fourier transform (STFT) method is applied to transform the raw monitoring data into time-frequency spectrograms (TFS). Three CNN architectures, i.e., small-sized CNN (S-CNN), middle-sized CNN (M-CNN), and large-sized CNN (L-CNN), are configured for trial calculation and the M-CNN model with excellent prediction accuracy and high computational efficiency is finally optioned for multiple damage detection of maglev rail joints. Results show that the rail joints in three different conditions (bolt-looseness-caused rail step, misalignment-caused lateral dislocation, and normal condition) are successfully identified by the proposed approach, even when using data collected from rail joints from which no data were used in the CNN training. The capability of the proposed method is further examined by using the data collected after the loosed bolts have been replaced. In addition, by comparison with the results of CNN using frequency spectrum and traditional neural network using TFS, the proposed TFS-CNN framework is proven more accurate and robust for multiple damage detection of maglev rail joints.

• Journal of the Korea institute for structural maintenance and inspection
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• v.26 no.6
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• pp.128-138
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• 2022

In this study, in order to apply ultra-high-strength concrete beams of 100 MPa or more manufactured by centrifugal molding as the superstructure of the avalanche tunnel, the purpose is to verify the structural safety of the corner rigid joint in which the centrifugal molded beam is integrated with the substructure, which is the negative moment area. A full-size specimen was manufactured, and loading tests and analysis studies were performed. In order to expect the same effect that the maximum moment occurs in the corner joint part of the upper slab end when the standard model of the avalanche tunnel is designed with a load combination according to the specification, a modified cantilever type structural model specimen was manufactured and the corner rigid joint was fixedly connected. A study was performed to determine the performance of the method and the optimal connection construction method. The test results demonstrated that the proposed connection system outperforms others. Despite having differences in joint connection construction type, stable flexural behavior was shown in all the tested specimens. The proposed method also outperformed the behavior of centrifugally formed beams and upper slabs. The behavior of the corner rigid joint analysis model according to the F.E. analysis showed slightly greater stiffness compared to the results of the experiment, but the overall behavior was almost similar. Therefore, there is no structural problem in the construction of the corner rigid joint between the centrifugally formed beam and the wall developed in this study.

• Journal of Korean Tunnelling and Underground Space Association
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• v.12 no.2
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• pp.193-200
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• 2010

Due to the characteristic of culvert structure, the standard section of the culvert has been established and applied in field. However, this becomes a limitation in selecting a section design corresponding to various field conditions although it can improve the design and applicability of culvert structure. In order to overcome this limitation, we have developed the design and application technology of culvert structure corresponding to the field conditions that various shapes of culvert structure can be covered by assembly of precast segments. Because the structural characteristics of assembling-type waterway culvert structure, the thickness of structure and amount of reinforcing rods can vary according to the fixation or internal hinge status in the connection part of precast segments. This has a strong influence on the applicability and economic efficiency of culvert structure. Accordingly, in order to suggest a reasonable modeling technique of segment connection parts, this study has conducted the field experiment and numerical analysis. According to the results of field experiment and numerical analysis, the slab, wall and base slab with mortar splice sleeves have shown that the assembling-type of waterway culvert structure behaves like an integrated structure.

• Informatization Policy
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• v.30 no.4
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• pp.113-137
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• 2023

This study aims to conduct the classification of Government as a Platform (GaaP) in a situation where the concept of GaaP can be diversely recognized. This is because inclusiveness and ambiguity in the concept of GaaP can hinder policy enforcement by working-level officials in the public sector. It drew the criteria for classification for GaaP based on literature and cases for GaaP. In the technical aspect, considering data as an overarching factor, the integrated system platform integrating the information system or websites of the public sector and the data platform as a single portal for open data to external stakeholders were sorted. In the governance aspect considering stakeholder as an overarching factor, the communication platform utilized for interaction between public and private sectors and the co-creation platform that encourages public-private partnership to create innovative outcomes were sorted. It suggested an actual implementation case and the policy implication according to each type of GaaP. Additionally, according to the classification of GaaP, it conducted contents analysis as to which type of GaaP the domestic Digital Platform Government belongs to based on its detailed assignment. Based on the classification of GaaP, it drew balanced implementation for various types of GaaP, plan for promoting the participation and collaboration of stakeholders, and necessity of restructuring and reinventing of the public sector as policy implications for the domestic digital platform government.