• Molecules and Cells
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• 제43권12호
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• pp.1011-1022
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• 2020

Cell type specification is a delicate biological event in which every step is under tight regulation. From a molecular point of view, cell fate commitment begins with chromatin alteration, which kickstarts lineage-determining factors to initiate a series of genes required for cell specification. Several important neuronal differentiation factors have been identified from ectopic over-expression studies. However, there is scarce information on which DNA regions are modified during induced pluripotent stem cell (iPSC) to neuronal progenitor cell (NPC) differentiation, the cis regulatory factors that attach to these accessible regions, or the genes that are initially expressed. In this study, we identified the DNA accessible regions of iPSCs and NPCs via the Assay for Transposase-Accessible Chromatin sequencing (ATAC-seq). We identified which chromatin regions were modified after neuronal differentiation and found that the enhancer regions had more active histone modification changes than the promoters. Through motif enrichment analysis, we found that NEUROD1 controls iPSC differentiation to NPC by binding to the accessible regions of enhancers in cooperation with other factors such as the Hox proteins. Finally, by using Hi-C data, we categorized the genes that directly interacted with the enhancers under the control of NEUROD1 during iPSC to NPC differentiation.

• The Plant Pathology Journal
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• 제32권6호
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• pp.528-536
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• 2016

Although many fungicides are registered for use to control powdery mildew on cucurbits, management of resistance to fungicides in pathogen populations still remains a major challenge. Two biopesticides Regalia SC and HMO 736 were evaluated in the greenhouse and field for their efficacy against powdery mildew in squash. In greenhouses, Regalia SC alone significantly (P < 0.05) reduced powdery mildew compared to the nontreated control, and was as effective as the chemical standard Procure 480SC (triflumizole). In alternation with Procure 480SC, Regalia SC demonstrated greater or equivalent effects on reducing the disease. HMO 736 alone showed varying levels of disease control, but alternating with Procure 480SC significantly improved control efficacy. In addition, application of Regalia SC or HMO 736 each in alternation with Procure 480SC significantly increased the chlorophyll content in leaves and the total fresh weight of squash plants, when compared with the water control, Regalia SC and HMO 736 alone. In field trials, application of Regalia SC and HMO 736 each alone significantly reduced disease severity in one of two field trials during the early stage of disease development, but not during later stages when disease pressure became high. Both Regalia SC and HMO 736 each applied in alternation with Procure 480SC significantly improved the control efficacy compared to Procure 480SC alone. Results from this study demonstrated that an integrated management program can be developed for powdery mildew in squash by integrating the biopesticides Regalia SC, HMO 736 with the chemical fungicide Procure 480SC.

• 대한의용생체공학회:의공학회지
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• 제26권5호
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• pp.265-270
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• 2005

A new Computer Integrated Surgical Robot system is composed of a surgical robot, a surgical planning system, and an optical tracking system. The system plays roles of an assisting surgeon and taking the place of surgeons for inserting a pedicle screw in spinal fusion. Compared to pure surgical navigation systems as well as conventional methods for spinal fusion, it is able to achieve better accuracy through compensating for the portending movement of the surgical target area. Furthermore, the robot can position and guide needles, drills, and other surgical instruments or conducts drilling/screwing directly. Preoperatively, the desired entry point, orientation, and depth of surgical tools for pedicle screw insertion are determined by the surgical planning system based on CT/MR images. Intra-operatively, position information on surgical instruments and targeted surgical areas is obtained from the navigation system. Two exemplary experiments employing the developed image-guided surgical robot system are conducted.

• The Plant Pathology Journal
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• 제24권4호
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• pp.407-416
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• 2008

Studies were conducted to determine the effects of temperature, solute potential and carbon source on the mycelial growth, sclerotia development, and apothecium production of an isolate of Sclerotinia sclerotiorum. Mycelial growth rate was greatest at $25^{\circ}C$ on potato dextrose agar (PDA) medium amended with up to 2% NaCl (${\psi}s{\leq}1.91\;MPa$) and thereafter, growth rate declined. The least number of sclerotia were produced at $20^{\circ}C$on both PDA and malt extract agar (MEA) amended with 8% NaCl (${\psi}s=6.62\;MPa$). With increasing temperature and decreasing solute potential the number and size of sclerotia were significantly reduced. The combined effect of temperature, solute potential and carbon source on sclerotia production were highly significant and had an impact on the development of the rind layer cells of sclerotia. These cells lacked a transparent cell wall which was surrounded by a compact melanized layer, and some of these cells appeared to be devoid of cell contents or were totally vacuolated. The survival of the sclerotia with increase in salinity and temperature appeared to affect melanization and the nature of the rind cells. The observations of this study re-enforces the need for an integrated disease management to control S. sclerotiorum.

• 보존과학연구
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• 통권23호
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• pp.77-93
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• 2002

There are several methods of pest control for the prevention of wooden cultural properties from damages caused by insects, especially termites. Those are as follows; physical control, chemical control, biological control, and integrated pest management. In Korea, we primarily use mvestigation of monitoring, fumigation, treatment of insecticidal and antiseptic chemicals, soil termiticide treatment, and termite colony elimination system. As damages by termites were detected at Eunghyanggak and Saundang of Haeinsa temple in June, 1998, we carried out monitoring with wooden device, in order to investigate damages of wooden building by termite and paths of termite invasion. According to the result of investigation of monitoring from1999 to 2002, we found that termites were inhabiting continually at forests around Haeinsa and that wooden buildings such as Saundang, Cheonghwadang were damaged by termites. So we thought that control methods such as soil termiticide treatment and termite colony elimination system would be effective. Especially, to investigate efficacy of termite colony elimination system, we carried out efficacy test of termite colony elimination system in Jongmyo during 17 months(2000.7.~2001.11.). The result of efficacy test showed that termite colony elimination system eliminated termite colonies gradually(over 12 months from installation of colony elimination system to elimination of termite colony).

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.175-179
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• 2004

Trichoderma harzianum-THN1 parasitising the egg masses of root knot nematode Meloidogyne incognita was isolated from galled mulberry roots and evaluated for its potential to control root knot disease. In pot experiments root galling was reduced and leaf yield increased significantly following soil treatment with T. harzianum-THN1. The extracts obtained from the soils inoculated with T. harzianum-THN1 drastically inhibited the hatching of nematode eggs and the effect was irreversible even after the eggs were transferred to fresh water. The fungus was equally effective in controlling the disease in nematode infested mulberry garden under field conditions which was significant over the most commonly used egg parasitic fungus Paecilomyces lilacinus. The disease reduction recorded with T. harzianum was on par with the plants treated with the nematicide Carbofuran. The results suggest that T. harzianum- THN1 could be used as a potent ecofriendly biocontrol agent against M. incognita in mulberry without any residual toxicity to silkworms. T. harzianum- THN1 can form an important component of integrated disease management package in mulberry cultivation.

• 미생물학회지
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• 제41권2호
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• pp.146-151
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• 2005

전분 이용이 가능한 산업용 Saccharomyces cerevisiae균주를 개발하기 위해 alcohol dehydrogenase 유전자 프로모터(ADClp)의 조절하에 발현되는 Aspergillus awamori glucoamylase cDNA 유전자(GA1)를 산업용 S. cerevisiae의 염색체에 도입하였다. 산업적 이용에 적합한 효모균주를 얻기 위해 세균 ampicillin 저항성 유전자가 제거되고 GA1 유전자와 선별 표지유전자로 S. cerevisiae aureobasidin A 저항성 유전자(AUR1-C)와 재조합 부위로 Tyretrotransposon $\delta$-서열이 포함된 integrative cassette를제조하였다. 이 $\delta-integrative$ cassette로 형질전환된 산업용 S. cerevisiae는 배지상에 glucoamylase를 생산 분비하였고 전환을 유일한 탄소원으로 하여 생장하였다. 형질전환체를 비선택배지에서 배양했을 매 삽입된 GA1유전자가 100세대까지 안정되게 유지되었다.

• BMB Reports
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• 제34권4호
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• pp.322-328
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• 2001

Excision and amplification of pre-determined, large genomic segments (taken directly from the genome of a natural host, which provides an alternative to conventional cloning in foreign vectors and hosts) was explored in human cells. In this approach, we devised a procedure for excising a large segment of human genomic DNA, the iNOS gene, by using the Cre/loxP system of bacteriophage P1 and amplifying the excised circles with the EBNA-1/oriP system of the Epstein-Barr virus. Two loxP sequences, each of which serves as a recognition site for recombinase Cre, were integrated unidirectionally into the 5'-UTR and 3'-UTR regions of the iNOS gene, together with an oriP sequence for conditional replication. The traps-acting genes cre and EBNA-1, which were under the control of a tetracycline responsive $P_{hcmv^*-1}$ promoter, were also inserted into the 5'-UTR and 3'-UTR regions of the iNOS gene, respectively, by homologous recombination. The strain carrying the inserted elements was stably maintained until the excision and amplification functions were triggered by the induction of cre and EBNA-1. Upon induction by doxycycline, Cre excised the iNOS gene that was flanked by two ZoxP sites and circularized it. The circularized iNOS gene was then amplified by the EBNA-1/oriP-system. With this procedure, approximately a 45.8-kb iNOS genomic fragment of human chromosome 17 was excised and successfully amplified in human cells. Our procedure can be used effectively for the sequencing of unclonable genes, the functional analysis of unknown genes, and gene therapy.

• 한국농공학회지
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• 제45권5호
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• pp.85-96
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• 2003

The Revised Universal Soil Loss Equation (RUSLE) has been used in over 100 countries to estimate potential long-term soil erosion from the field. However, the RUSLE estimated soil erosion cannot be used to estimate the sediment delivered to the stream networks. For an effective erosion control, it is necessary to compute sediment delivery ratio (SDR) for watershed and sediment yield at watershed outlet. Thus, the Sediment Assessment Tool for Effective Erosion Control (SATEEC) was developed in this study to compute the sediment yield at any point in the watershed. To compute spatially distributed sediment yield map, the RUSLE was first integrated with the ArcView GIS and three area based sediment delivery ratio methods were incorporated in the SATEEC. The SATEEC was applied to the Bangdong watershed, Chuncheon, Gangwon Province to demonstrate how it can be used to estimate soil loss and sediment yield for a watershed. The sediment yield using USDA SDR method is 8,544 ton/year and 4,949 ton/year with the method by Boyce. Thus, use of watershed specific SDR is highly recommended when comparing the estimated sediment yield with the measured sediment data. The SATEEC was applied with hypothetical cropping scenario and it was found that the SATEEC can be used to assess the impacts of different management on the sediment delivered to the stream networks and to find the sediment source areas for a reach of interest. The SATEEC is an efficient tool to find the best erosion control practices with its easy-to-use interface.

• 한국물환경학회지
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• 제26권4호
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• pp.637-647
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• 2010

The Universal Soil Loss Equation (USLE)-based modeling systems have been widely used to simulate soil erosion studies. However the GIS-based USLE modeling systems have limitation in gully erosion evaluation which is one of the most important factor in soil erosion estimation. In this study, the integrated soil erosion evaluation system using with Sediment Assessment Tool for Effective Erosion Control (SATEEC) system, nLS and Unit Stream Power-based Erosion/Deposition (USPED) model was developed to simulate gully erosion. Gully head location using nLS model, USPED for gully erosion, and the SATEEC estimated sheet and rill erosion were evaluated and combined together with the integrated soil erosion evaluation system. This system was applied to the Haean-myeon watershed, annual average sediment-yield considering sheet, rill and gully erosion was simulated as 101,933 ton/year at the study watershed. if the integrated soil erosion evaluation system is calibrated and validated with the measured data, this system could be efficiently used in developing site-specific soil erosion best management system to reduce soil erosion and muddy water inflow into the receiving waterbody.