• Animal Bioscience
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• v.35 no.11
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• pp.1635-1648
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• 2022

Thermal stress due to extreme changes in the thermal environment is a critical issue in cattle production. Many previous findings have shown a decrease in feed intake, milk yield, growth rate, and reproductive efficiency of cattle when subjected to thermal stress. Therefore, selecting thermo-tolerant animals is the primary goal of the efficiency of breeding programs to reduce those adverse impacts. The recent advances in molecular genetics have provided significant breeding advantages that allow the identification of molecular markers in both beef and dairy cattle breeding, including marker-assisted selection (MAS) as a tool in selecting superior thermo-tolerant animals. Single-nucleotide polymorphisms (SNPs), which can be detected by DNA sequencing, are desirable DNA markers for MAS due to their abundance in the genome's coding and non-coding regions. Many SNPs in some genes (e.g., HSP70, HSP90, HSF1, EIF2AK4, HSBP1, HSPB8, HSPB7, MYO1A, and ATP1A1) in various breeds of cattle have been analyzed to play key roles in many cellular activities during thermal stress and protecting cells against stress, making them potential candidate genes for molecular markers of thermotolerance. This review highlights the associations of SNPs within these genes with thermotolerance traits (e.g., blood biochemistry and physiological responses) and suggests their potential use as MAS in thermotolerant cattle breeding.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.155-155
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• 2023

국내 이상기후로 인해 여름철 하천과 호소에서 빈번하게 발생되는 조류의 과대성장이 매년 문제가 되고 있다. 하천 조류는 일차생산자로서 매우 중요한 역할을 하지만, 하천조류 중 유해남조류가 생장하면서 발생하는 악취 유발 물질과 독성물질의 배출로 문제를 야기하고 있다. 국내에서는 조류경보제와 수질예보제를 시행하여 국민의 안전을 도모하기 위해 최선을 다하고 있으며, 발령 기준은 유해남조류세포수에 따라 발령이 되기 때문에 유해남조류 측정은 매우 중요하다. 현재 조류의 분석방법은 현장에서 조류샘플을 채취하고 실험실에서 현미경을 통해 조류샘플을 검경하고 녹조류, 남조류, 규조류의 세포수 또는 우점종을 산정한다. 조류검경은 개인의 역량에 따라 오차가 생길수 있고 시간이 많이 소요된다. 최근 많은 연구자들이 이런 문제를 해결하기 위해 인공위성, 광학영상, 초분광영상 등을 통해 녹조류와 남조류 대체 인자인 Chlorophyll, Phycocyanin을 통해 조류농도를 추정하고 있으나, 조류세포수 분석에 대한 연구는 미비한 실정이다. 본 연구에서는 매년 조류 발생으로 문제가 되는 물금취수장 인근 하천에서 발생한 조류를 채취하고 조류검경을 통해 얻은 남조류세포수와, LISST-HAB를 통해 얻은 Phycocyanin농도, 초분광영상을 활용한 조류스펙트럼 데이터를 통해 남조류세포수 추정하고 남조류세포수와 비교분석을 진행하였다. 본 연구를 통해 조류측정 원격탐사 연구의 기초자료로 제공하고자 한다.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.344-344
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• 2021

세계적으로 기후변화 현상으로 인해 극심한 이상 가뭄이 빈번하게 발생하고 있다. 물 공급 소외지역의 경우 가뭄으로 인해 주민들이 불편을 겪고 있으며, 특히 마을상수도 및 소규모수도시설에 의존하는 최상류부 유역의 취수원은 상대적으로 가뭄에 취약하다. 본 연구의 대상 지역은 강원도 춘천시 물로천의 제1지류 유역으로 주민들이 하천 계곡수를 생활용수의 주 공급원으로 사용하고 있다. 해당지역은 갈수기에 생활용수 부족 문제를 겪고 있는 지역으로 가뭄 대응을 위한 구조적 대책으로 다단식 Sand댐을 설치할 예정이다. 현장조사 결과 물로천의 물은 취수조로 일부 유입되며, 자갈 등을 이용하여 부유물 등이 필터링되어 물탱크로 흘러내리며 중력에 의해 각 가정으로 자연 유하하는 것으로 나타났다. 본 연구에서는 대상지역의 하천, 취수조, 물탱크 3개 지점에 압력식 자동 수위 관측기를 설치하였으며, 2시간 간격의 수위를 측정할 수 있도록 설정하여 매달 유속 측정을 통해 수위-유량 관계 곡선식을 바탕으로 유량을 산정하였다. 도출된 일별 유량 자료와 SWAT 모형의 매개변수 검·보정한 후 이를 장기간으로 확장 적용하여 약 10년간의 장기 유출량을 산정하였다. 일별 장기 유출량 자료를 통해 평균 유량 감수곡선과 감수곡선식을 도출하였고, 무강우에 따른 최대 공급가능일수를 산정하였다. 본 연구에서는 하천-취수원 연계 모니터링 및 하천 유량에 따른 공급가능일수 등을 제시하여 가뭄 취약지역의 주민 불편을 최소화하고 가뭄에 대응할 수 있을 것으로 판단된다. 최종적으로 추후 Sand댐 건설 전후 비교 자료로 사용될 수 있으며, 소규모수도시설의 가뭄 관리를 위한 모니터링 체계 및 대응 방안을 구축하였다.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.61-70
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• 2008

The object of this experiment was to investigate the food habits of sika deer fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources; 5 species(Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass), legume sources; 5 species(Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources; 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources; 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea(Roxb.) Wight and Rumex crispus), and native grass sources; 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Five sika deer were selected which had nearly the same body weight(average 92.5kg) as experimental animal. Experimental roughages were prepared by 0.5kg(DM basis) per 1 species fed to sika deer, and the experiment was carried out from 08:00 to the next day 06:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. The sika deer ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species ranking by sika deer was observed like this: Quercus aliena Blume, Prunus jamasakura Sieb, Quercus serrata Thunb. and Trifolium pratense, respectively, and the lower intake species was Phragmites comunis Trin. On the other hand, compared to each roughage source, total intake amount by sika deer was showed as browse(36.5%), Legumes(34.2%), grasses(14.0%), native grasses(13.5%) and weeds(1.8%), respectively. Based on the result, the food habits of sika deer seems to be closer to typically browser type.

• Journal of Animal Science and Technology
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• v.48 no.6
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• pp.847-860
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• 2006

This study investigated the effects of feeding Charolais steers on diets rich in either n-6 or n-3 polyunsaturated fatty acids (PUFA) and time on feed (TOF) on muscle fatty acid composition and content. Twenty eight steers were fed on ad libitum forage and one of two concentrates varying in the source of fat; soya (high in C18:2 n-6) or whole linseed (high in C18:3 n-3) for either 60 or 90 days in IGER (Institute of Grassland and Environmental Research, UK). The concentrates were fed at approximately 0.73 of total DM intake. TOF influenced carcass weight, conformation and fatness scores, which were higher at 90 v. 60 days (P<0.05). Diet did not affect total fatty acid content of neutral lipid in m. longissimus thoracis but feeding linseed increased total phospholipid fatty acid by approx- imately 15%(P<0.05). Linseed increased the amount and proportion of C18:3 n-3 (P<0.001) and the proportion of CLA (cis-9, trans-11 conjugated linoleic acid), while soya increased the content (P<0.05) and proportion (P<0.001) of C18:2 n-6 in muscle neutral lipid. In muscle phospholipid, linseed significantly increased the amount of CLA, C18:3 n-3 and its longer chain derivatives as well as C14:0, C16:0, C18:0. C18:1 trans and C18:2 n-6. The amount and proportion of C18:2 n-6 and its longer chain C20 derivatives were higher on feeding soya. TOF (90 v. 60 day) increased the content of C14:0, C16:0, C16:1, CLA, C18:1 n-9, C18:2 n-6 and C18:3 n-3 in muscle neutral lipid. The P:S was not affected by diet or TOF. The ratio of C18:2 n-6 : C18:3 n-3 and sum of n-6 : n-3 fatty acids were higher in muscle from animals fed on linseed v. soya (P<0.001). The study indicates that the PUFA composition of beef muscle may be significantly modified by feeding contrasting dietary lipids, soya vs. linseed. Feeding linseed produced a better balance of muscle fatty acids, more in line with current nutritional recommendations with a lower C18:2 n-6:C18:3 n-3 ratio associated with higher muscle content of C18:3 n-3 and C20:5 n-3 and CLA and lower C20:4 n-6.

• Journal of Embryo Transfer
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• v.21 no.4
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• pp.331-338
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• 2006

This survey was carried out to investigate the occurrence of reproductive disorders in Korean native cattle (Hanwoo). The general management status such as barn type, feed intake, parity and type of reproductive disorders were examined in the Hanwoo farms. In this survey, the total incidence of reproductive disorders that was assessed from 54 Hanwoo farms was 11.6% (73/ 631). We first examined the incidence of reproductive disorders following herd size per farm. The rates of reproductive disorders in under 10 heads, 10 to 20 heads and over 20 heads were 25.2%, 9.4% and 8.3%, respectively (P<0.05). The rate of reproductive disorders in tie stall barn was significantly (P<0.05) higher than that in free stall barn (14.9% vs. 8.1%). The major types of reproductive disorders were follicular cysts (21.8%), fat necrosis (21.8%), and repeat breeding (17.8%). The rate of reproductive disorders assessed by the parity tended to decrease as the parity increased. The relationship between incidence of reproductive disorders and body condition score (BCS) was investigated in 203 Hanwoo. The incidence of reproductive disorder was 80.0%, 8.8%, 9.4%, 10.0%, 33.3% and 57.1% at 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0 of BCS, respectively. These data show that the increase of herd size, barn type and BCS affect the incidence of reproductive disorders such as follicular cysts, fat necrosis and repeat breeding in Hanwoo.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.6
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• pp.812-820
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• 2002

An experiment was conducted on 190 progeny (winter -74; summer -59; rainy -57) of 12 Grey Giant rabbits (10 female +2 males), to assess the effect of different seasons in a year, on their reproductive, growth and productive performances along with feed efficiency, under sub-temperate Himalayan conditions. The daily meteorological attributes recorded during winter (October to March), summer (April to June) and rainy (July to September) seasons, and analysed were minimum and maximum temperature, relative humidity and rainfall. Various biological parameters recorded were doe weights at mating and kindling, litter size at birth, litter weight at birth, kit mortality, litter size at weaning, litter weight at weaning, weekly body weight up to 98 d and weaner mortality. Individual weight gains, dressing percentages, meat weights, liver weights, raw-pelt weights, processed pelt weights and processed pelt areas at slaughter on d 84 and 98, respectively were also recorded. The feed and fodder compositions and their nutritive values during different seasons were also analysed. Average ambient temperature during winter, summer and rainy seasons were $13.2{\pm}2.8$, $22.4{\pm}3.7$ and $24.8{\pm}2.3^{\circ}C$, respectively. The average relative humidity and total rainfall for winter, summer and rainy seasons were $68.9{\pm}1.5$% and $48{\pm}26.6$mm, $66.3{\pm}4.8$% and $125.6{\pm}56.8$ mm, and $77.3{\pm}1.3$% and $116.3{\pm}90.4$ mm, respectively. The weight of doe at mating and kindling, litter size at birth, litter weight at birth and litter size at weaning were comparatively higher whereas litter weight at weaning was significantly (p<0.05) higher during winter as compared to summer and rainy seasons. The kit mortality was significantly (p<0.05) higher during winter while the weaner mortality was significantly (p<0.05) higher during rainy season. At 84 d, the live weight per doe, slaughter weight, dressing percentage and liver weight were significantly (p<0.05) higher during winter than summer and rainy. Similarly, the gain in weight and meat weight at 84 and 98 d were significantly (p<0.05) higher during winter. The weight of raw pelt and processed pelt were recorded significantly (p<0.05) higher during winter while no difference in the area of processed pelts during different seasons could be observed. No difference in the biological performance could be observed between sexes in any of the seasons. Roughage analysis revealed comparatively higher crude protein percent and lower crude fibre percent during summer and rainy seasons than in winter. The roughage dry matter intake was comparatively higher during summer and rainy seasons vis-a-vis constant amount of concentrate supplied during all the three seasons. The digestibilities of dry matter was significantly (p<0.05) lower, whereas that of crude fiber, acid detergent fibre and cellulose were negative during winter. Interestingly, the feed:gain was exceedingly well during winter than in other seasons and it is concluded that it was the best season for production of rabbits under sub-temperate Himalayan conditions.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.7
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• pp.1011-1017
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• 2002

Conjugated linoleic acids (CLA) have been shown to decrease body fat content of individually-housed pigs but little is known about the responses under commercial conditions. Two studies were conducted to evaluate the effect of CLA under commercial conditions using contemporary genotypes. The experimental designs were similar between the two sites. Briefly, the studies were 2${\times}$2 factorial designs with the respective factors being sex (boar and gilt) and supplemental dietary CLA (0 and 4 g/kg). The studies involved 16-20 pens of pigs with 4-5 pens of each sex${\times}$CLA group. The first study was conducted with 144 pigs in 16 pens consuming a pelleted feed for 6 weeks at Bunge Meat Industries, Corowa, NSW. In the second study, 160 pigs were obtained from a commercial source and put into 20 pens in simulated commercial conditions and fed a mash diet for 7 weeks at Medina Research Station, WA. In Study 2 some aspects of meat quality were also investigated. Data from Study 1 showed that, although CLA had no significant effect upon feed intake and daily gain, the small changes in both resulted in a reduction in (-0.10 g/g, p=0.10) feed conversion ratio (FCR). While there was no significant effect of CLA on ultrasonic backfat depths, there was a significant decrease in carcass P2 (-1.0 mm, p=0.014) and estimated carcass fat (-7 g/kg, p=0.049). In the study conducted at Medina CLA had no significant effect upon feed intake, feed:gain or most measures of back fat. The exception was that dietary CLA decreased the rate of accumulation of fat at the shoulder, particularly in gilts, resulting in a significantly lower amount of shoulder fat at slaughter (-1.3 mm, p=0.044). CLA tended to increase dressing percentage although this was not significant (+0.5%, p=0.14). Meat from CLA treated pigs tended to be darker (p=0.12) and had a higher ultimate pH (p=0.06). These data suggest that under commercial conditions dietary CLA can improve growth performance and decrease P2 in pigs of an improved genotype, particularly gilts.

• Analytical Science and Technology
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• v.29 no.6
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• pp.249-254
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• 2016

Looking at the current status of beverages sold from vending machines in subway stations in Seoul, it was found that carbonated beverages accounted for the highest percentage of beverages on sale in vending machines, representing 36 % of all beverages sold. Survey respondents purchased carbonated drinks or sports drinks containing high levels of sugar to quench their thirst. Investigation of the sugar content of beverages frequently sold in vending machines showed that a serving of carbonated beverage contained an average of 30.4 g of sugar, or as much as 50.1 g of sugar (equivalent 10 to 17 cubes; 1 cube = 3 g of sugar). This amount corresponded, on average, with 60 % of the WHO recommended daily sugar intake (50 g). Surprisingly, with certain carbonated beverages, drinking a single can is equivalent to consuming the total recommended daily sugar intake of 50 g.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.2
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• pp.119-128
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• 2008

The object of this experiment was to investigate the food habits of Korean native goats fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources: 5 species (Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass, legume sources: 5 species (Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources: 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources: 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea (Roxb.) Wight and Rumex crispus), and native grass sources: 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Ten Korean native goats were selected which had nearly the same body weight(average 30.6kg, 남) as experimental animals. Experimental roughages were prepared by 0.5kg per 1 species fed to Korean native goats, and the experiment was carried out from 08:00 to 20:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period (p<0.05). The Korean native goats ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species order by Korean native goats was observed like this: Quercus serrata Thunb., Quercus aliena Blume, Trifolium repens, and Pinus densiflora and Artemisia princeps Pampanini which was lower intake compared to other domestic herbivores. On the other hand, compared to each roughage source, total intake amount by Korean native goats was showed as browse(29.9%), legumes(23.0%), weeds(21.6%), grasses(21.5%) and native grasses(4.0%), respectively. Based on the result, the food habit of Korean native goats seems to be closer to browser type.