• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.489-498
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• 2013

A new Bacillus strain degrading starch, named Bacillus sp. UEB-S, was isolated from a southern Tunisian area. Amylase production using solid-state fermentation on millet, an inexpensive and available agro-resource, was investigated. Response surface methodology was applied to establish the relationship between enzyme production and four variables: inoculum size, moisture-to-millet ratio, temperature, and fermentation duration. The maximum enzyme activity recovered was 680 U/g of dry substrate when using $1.38{\times}10^9$ CFU/g as inoculation level, 5.6:1 (ml/g) as moisture ratio (86%), for 4 days of cultivation at $37^{\circ}C$, which was in perfect agreement with the predicted model value. Amylase was purified by Q-Sepharose anion-exchange and Sephacryl S-200 gel filtration chromatography with a 14-fold increase in specific activity. Its molecular mass was estimated at 130 kDa. The enzyme showed maximal activity at pH 5 and $70^{\circ}C$, and efficiently hydrolyzed starch to yield glucose and maltose as end products. The enzyme proved its efficiency for digesting raw cereal below gelatinization temperature and, hence, its potentiality to be used in industrial processes.

• Journal of the Korean Institute of Telematics and Electronics
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• v.27 no.11
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• pp.1-8
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• 1990

We made a 12-Laser Diode Array consisting of 12 Graded Index Separate Confinement (GRINSCH) InGaAs/Inp Buried Heterostructure 4 Quantum Well Laser Diodes and examined the potential of controlling lasing operation of each laser diode by the voltage to its electroabsorption region. Using Si V-Groove with 12 V-grooves, a 12-Laser Diode Array, and 12 optical fibers, we investigated the various characteristics of each laser diode by changing the voltage to its electro-absorption region. Finally, we thought over the promising way of implementing optical local area communication between electric circuit boards or between subscribers and a central office using a 12-Laser Diode Array, Si V-groove, and optical fibers.

• Biomolecules & Therapeutics
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• v.22 no.6
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• pp.477-490
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• 2014

Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.

• International Journal of Computer Science & Network Security
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• v.21 no.1
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• pp.119-124
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• 2021

Elastic Optical Networks (EONs) allow to solve the high demand for bandwidth due to the increase in the number of internet users and the explosion of multicast applications. To support multicast applications, network operator computes a tree-shaped path, which is a set of optical channels. Generally, the demand for bandwidth on an optical channel is enormous so that, if there is a single fiber failure, it could cause a serious interruption in data transmission and a huge loss of data. To avoid serious interruption in data transmission, the tree-shaped path of a multicast connection may be protected. Several works have been proposed methods to do this. But these works may cause the duplication of some resources after recovery due to a link failure. Therefore, this duplication can lead to inefficient use of network resources. Our work consists to propose a method of protection that eliminates the link that causes duplication so that, the final backup path structure after link failure is a tree. Evaluations and analyses have shown that our method uses less backup resources than methods for protection of a multicast connection.

• Computers and Concrete
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• v.31 no.2
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• pp.163-171
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• 2023

Face gear transmission is widely used in aerospace shunt-confluence transmission system. Tooth wear is one of the main factors affecting its bearing transmission performance. Furthermore, the installation errors of face gear are inevitable. In order to study the wear mechanism of face gear tooth surface with installation errors, based on tooth contact analysis numerical method and Archard wear theory, the UMESHMOTION subroutine in ABAQUS is developed.Combining with Arbitrary Lagrangian-Eulerian adaptive mesh technology, the finite element mesh wear model of abraded face gear pair is established.The preprocessing conditions are set to generate the inp files.Then,the inp files for each corner are imported and batch processed in ABAQUS.The loading tooth contact problem at each rotation angle is solved and the load distribution coefficient among gear tooth, tooth root bending stress, tooth surface contact stress and loaded transmission error are obtained. Results show that the tooth root wear is the most serious and the wear at the pitch cone is close to 0.The wear law of tooth surface along tooth width direction is convex parabola and the wear law along tooth height direction is concave parabola.

• International conference on construction engineering and project management
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• 2011.02a
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• pp.74-83
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• 2011

This paper recognized a need in the architecture, engineering, and construction industry for new programs and methods of producing reliable energy simulations using BIM (Building Information Modeling) technology. Current methods and programs for running energy simulations are not very timely, difficult to understand, and lack high interoperability between the BIM software and energy simulation software. It is necessary to improve on these drawbacks as design decision are often made without the aid of energy modeling leading to the design and construction of non-optimized buildings with respect to energy efficiency. The goal of this research project is to develop a new methodology to produce energy estimates from a BIM model in a more timely fashion and to improve interoperability between the simulation engine and BIM software. In the proposed methodology, the extracted information from a BIM model is compiled into an INP file and run in a popular energy simulation program, DOE-2, on an hourly basis for a desired time period. Case study showed that the application of this methodology could be used to expediently provide energy simulations while at the same time reproducing the BIM in a more readably three dimensional modeling program. With the aid of an easy to run and easily understood energy simulation methodology, designers will be able to make more energy conscious decisions during the design phase and as changes in design requirements arise.

• ETRI Journal
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• v.4 no.1
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• pp.3-8
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• 1982

성장된 GaInAsP/InP DH wafer로부터 상온에서 duty 5%까지 Pulse 동작이 가능한 전면전극(broad contact) 및 stripe 구조 LD(711이저 다이오드)를 제작하였다. 또 이렇게 제작된 LD의 I-V특성, 1-L 특성 및 발진파장 등을 조사하기 위해 LD 구동회로 및 Ge 태양전지와 Ge-APD를 이용한 광 검출회로를 제작하였다. 이들을 이용해 제작한 LD의 특성을 조사한 결과 stripe 구조 LD인 경우 발진개시전류($I_th$)가 900mA, 발진파장이 $1.29\mum$, 파장반치폭(FWHM)이 $60\AA$였으며 $1.33I_th$까지 kink 없이 동작이 가능하였다.

• The Korean Journal of Physiology and Pharmacology
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• v.23 no.5
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• pp.357-366
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• 2019

$G{\alpha}_q$-coupled receptor stimulation was implied in the activation process of transient receptor potential canonical (TRPC)1/4 and TRPC1/5 heterotetrameric channels. The inactivation occurs due to phosphatidylinositol 4,5-biphosphate ($PI(4,5)P_2$) depletion. When $PI(4,5)P_2$ depletion was induced by muscarinic stimulation or inositol polyphosphate 5-phosphatase (Inp54p), however, the inactivation by muscarinic stimulation was greater compared to that by Inp54p. The aim of this study was to investigate the complete inactivation mechanism of the heteromeric channels upon $G{\alpha}_q$-phospholipase $C{\beta}$ ($G{\alpha}_q-PLC{\beta}$) activation. We evaluated the activity of heteromeric channels with electrophysiological recording in HEK293 cells expressing TRPC channels. TRPC1/4 and TRPC1/5 heteromers undergo further inhibition in $PLC{\beta}$ activation and calcium/protein kinase C (PKC) signaling. Nevertheless, the key factors differ. For TRPC1/4, the inactivation process was facilitated by $Ca^{2+}$ release from the endoplasmic reticulum, and for TRPC1/5, activation of PKC was concerned mostly. We conclude that the subsequent increase in cytoplasmic $Ca^{2+}$ due to $Ca^{2+}$ release from the endoplasmic reticulum and activation of PKC resulted in a second phase of channel inhibition following $PI(4,5)P_2$ depletion.

• Molecules and Cells
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• v.42 no.5
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• pp.397-405
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• 2019

The regulatory role of long noncoding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) in both cancerous and noncancerous cells have been widely reported. This study aimed to evaluate the role of lncRNA GAS5 in heart failure caused by myocardial infarction. We reported that silence of lncRNA GAS5 attenuated hypoxia-triggered cell death, as cell viability was increased and apoptosis rate was decreased. This phenomenon was coupled with the down-regulated expression of p53, Bax and cleaved caspase-3, as well as the up-regulated expression of CyclinD1, CDK4 and Bcl-2. At the meantime, the expression of four heart failure-related miR-NAs was altered when lncRNA GAS5 was silenced (miR-21 and miR-142-5p were up-regulated; miR-30b and miR-93 were down-regulated). RNA immunoprecipitation assay results showed that lncRNA GAS5 worked as a molecular sponge for miR-142-5p. More interestingly, the protective actions of lncRNA GAS5 silence on hypoxia-stimulated cells were attenuated by miR-142-5p suppression. Besides, TP53INP1 was a target gene for miR-142-5p. Silence of lncRNA GAS5 promoted the activation of PI3K/AKT and MEK/ERK signaling pathways in a miR-142-5p-dependent manner. Collectively, this study demonstrated that silence of lncRNA GAS5 protected H9c2 cells against hypoxia-induced injury possibly via sponging miR-142-5p, functionally releasing TP53INP1 mRNA transcripts that are normally targeted by miR-142-5p.

• Geomechanics and Engineering
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• v.29 no.2
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• pp.155-170
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• 2022

An accurate analysis of structures supported on soft soils and subjected to seismic loading requires the consideration of the soil-foundation-structure interaction. An important aspect of this interaction lies with the energy dissipation due to soil material damping. Unlike advanced constitutive models that can induce energy loss, the use of simple elastoplastic constitutive models requires additional damping. The frequency dependent Rayleigh damping is a formulation that is frequently used in dynamic analysis. The main concern of this formulation is the correct selection of the target damping ratio and the frequency range where the response is frequency independent. The objective of this study is to investigate the effects of the Rayleigh damping parameters in soil-pile-structure and soil-inclusion-platform-structure systems in the presence of soft soil under seismic loading. Three-dimensional analyses of both systems are carried out using the finite difference software Flac3D. Different values of target damping ratios and minimum frequencies are utilized. Several earthquakes are used to study the influence of different excitation frequencies in the systems. The soil response in terms of accelerations, displacements and strains is obtained. For the rigid elements, the results are presented in terms of bending moments and normal forces. The results show that when the frequency of the input motion is close to the minimum (central) frequency in the Rayleigh damping formulation, the overdamping amount is reduced, and the surface spectral acceleration of the analyzed pile and inclusion systems increases. Thus, the bending moments and normal forces throughout the piles and inclusions also increase.