• Korean Journal of Plant Resources
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• v.11 no.3
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• pp.296-300
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• 1998

When medicinal mushroom, Poria cocos, is cultured , inoculation method of spawn is cross slice inoculation of which the both sides of pine tree were peeled and spawn of P.cocos was inoculated. However, this method required lots of inoculation amount. This study was carried out to improve the culturing method of P. cocos. A good growth of P.cocos was observed in MCM(mushroom complete medium), showing proper mycelia growth and density. In inoculation amount, conventional method(cross slice inoculation) requires 20 bottles of spawn. In contrast, short log method required 8 bottles of spawn and drilling inoculation method 2~3 bottles, which could save by 60% and 85-90% respectively. In the selectrion of tree species, pine and larch had better condition for spawn culture and sclerotia formation condition.In terms of yield , pine was 33.7kg/3.3$m^2$. In the yield of pine, conventional method was 23.4kg/3.3$m^2$, drilling inoculation 29.4kg/3.3$m^2$, short log inoculation 31.7kg/3.3$m^2$, therefore drilling inoculation could increase by 25% and short log inoculation 35%, In addition, management cost was also saved.

• Plant Disease and Agriculture
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• v.5 no.2
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• pp.90-94
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• 1999

Effect of inoculum concentration inoculation method and plant age on development of clubroot disease of Chinese cabbage seedling were examined in growth chambers. Root galls were developed at the concentration of 105 resting spore or above per ml of incoulum and as the inoculum concentration became higher rate of development of root galls was faster. In the plants with root gall development fresh weight of above ground parts was reduced to 30-44% of that of healthy plants but root weight increased by 4-10 times. Growth of diseased plants was greatly reduced as compared to healthy plants. Planting in the diseased soil as a inoculation method was most effective for disease development showing uniform infections but time of initial root gall development was delayed by root soaking inoculation. Some plants inoculated by soil drenching method did not develop root galls. However root gall enlargement after its initial formation did not differ greatly among inoculation methods. Nine-day-old seedlings showed poor development of root gall but 16-days-old seedlings was found to be most adequate for inoculation for gall development.

• Journal of the Korean Society of Tobacco Science
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• v.24 no.1
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• pp.27-31
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• 2002

Three kinds of inoculation methods, capillary, root cutting and dipping were compared for an efficient way to screening the resistant tobacco variety against bacterial wilt, Ralstonia solanacearum. The pricking a capillary tube contained the pathogenic bacterial suspension(10$^{7}$ cfu/$m\ell$) to an axillary bud of each tobacco plant showed different resistance well between varieties. The less period was required in inoculating work and in disease development for the inoculation method used with capillary tube than for two other inoculation methods tested also.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.5
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• pp.373-378
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• 2013

Information on the effective application method of arbuscular mycorrhizal fungi (AMF) inoculum is still inadequate. This work was performed to assess two AMF inoculation methods (dispersed and proximate) on root colonization of sorghum-sudangrass hybrid (Sorghum bicolor L.). In dispersed inoculation method, spores were inoculated in 2 kg pots of soil in which 5 day-old seedlings were transplanted and maintained for 50 days. In the proximate inoculation method, spores were first introduced in 500 mL pots where seeds were sown. After 10 days, the seedlings with the 500 mL soil were transferred to 2 kg pots without disturbing the contents. After 50 days of growth, root colonization and arbuscule abundance significantly increased (over 100%) in proximate method of inoculation. Moreover, sorghum-sudangrass hybrid had higher shoot growth (182.5 cm) and Glomalin related soil protein (GRSP) production in proximate method. Nutrient accumulation, particularly total nitrogen (82.61 mg $plant^{-1}$), was also found to be higher in proximate method of inoculation. Our results demonstrate that the proximate method of inoculation may improve the early stage mycorrhizal symbiosis and inoculum performance in Saemangeum reclaimed soil.

• Korean Journal Plant Pathology
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• v.1 no.2
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• pp.115-121
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• 1985

To improve methods of screening rice cultivars with field resistance to bacterial leaf blight, testing plant inoculation and neighbor plant inoculation were compared by using 33 rice cultivars. In the testing plant inoculation method, field resistance was evaluated by measuring the leaf areas diseased on the new leaves expanded after the inoculation. Varietal differences in field resistance were recognized more clearly by the testing plant inoculation method than by the neighbor plant inoculation method. Highly significant correlation was observed between the results of the two methods. Some rice cultivars such as, Seomjin, Hangangchal, Taebaeg, Samgang, Milyang 42, Asominori, Java 14, Chugoku 45 and 70X-46 showed remarkable field resistance to bacterial leaf blight. The testing plant inoculation method appeared desirable for screening rice cultivars for the qualitative and field resistance to bacterial leaf blight because of using less labor and less field area than neighbor plant inoculation.

• The Plant Pathology Journal
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• v.19 no.5
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• pp.257-259
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• 2003

This report described a simple, inexpensive, faster, and effective graft inoculation method for the artificial transmission of Mungbean yellow mosaic virus (MYMV). Success of grafting and disease transmission was 100％ in this method. Screening of mungbean germplasm using this method will prevent the chance of escape infection, probably as a consequence of non-preference mechanism and loss of vector infectivity. The grafting method described here is applicable to both screenhouse and field trials.

• Mycobiology
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• v.30 no.4
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• pp.208-212
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• 2002

For comparison of mycelial colonization of Phellinus linteus on logs, several techniques of inoculation were tested; sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich inoculation. The initial mycelial growth and the full mycelial colonization of P. linteus in logs were the best in case of 20 cm logs under the condition of 42% moisture content. Also, the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after $5{\sim}6$ months of incubation was the best for formation of basidiocarps of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at $31{\sim}35^{\circ}C$ and over 96% of relative humidity.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.3
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• pp.291-300
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• 2018

The purpose of the present work is to establish an ultra-minimal invasive percutaneous puncture inoculation method for a VX2 orthotopic lung cancer rabbit model with fewer technical difficulties, lower mortality of rabbits, a higher success rate and a shorter operation time, to evaluate the growth, metastasis and apoptosis of tumor by CT scans, necropsy, histological examination, flow cytometry and immunohistochemistry. The average inoculation time was 10-15 min per rabbit. The tumorbearing rate was 100%. More than 90% of the tumor-bearing rabbits showed local solitary tumor with 2-10 mm diameters after two weeks post-inoculation, and the rate of chest seeding was only 8.3% (2/24). The tumors diameters increased to 4-16 mm, and irregularly short thorns were observed 3 weeks after inoculation. Five weeks post-inoculation, the liquefaction necrosis and a cavity developed, and the size of tumor grew further. Before natural death, the CT images showed that the tumors spread to the chest. The flow cytometry and immunohistochemistry indicated that there was less apoptosis in VX2 orthotopic lung cancer rabbit model compared to chemotherapy drug treatment group. Minimal invasive percutaneous puncture inoculation is an easy, fast and accurate method to establish the VX2 orthotopic lung cancer rabbit model, an ideal in situ tumor model similar to human malignant tumor growth.

• Korean Journal Plant Pathology
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• v.14 no.4
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• pp.339-344
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• 1998

Since the leaf inoculation procedures are time-consuming and require considerable growth chamber space, a rapid dioassay method for screening of pathogenicity of Didymella bryoniae, a casual agent of gummy stem blight in watermelon, was established in this paper. The method produced reliable results within 8 days ( 5 days for growing seedlings and 3 days for rapid disease response in the seedlings). After contaminants in the root of 4~5 day-old seedlings had been washed using sterilized water, 5 seedlings were dipped into a vial containing 12 ml of conidial suspension (106 cells/ml). After the vials were placed in a growth chamber (22$^{\circ}C$, RH 50%, 14hr light/10hr darkness) for 3 days, susceptibility and resistance of cultivars were determined by the degree of disease response on cotyledon. The result of obtained by the dip-inoculation method was well coincided with the results by the leaf inoculation procedures and the result that had been observed for several years in the field. Screening of collected watermelon cultivars by the dip-inoculation method revealed that all the 21 domestic cultivars collected were susceptible and only 3 foreign cultivars (PI 189225, PI 482322 and IT 188207) were resistant among 18 cultivars A cucumber cultivar (Marketer) and bitter cucumber were proven to be resistant against the D. bryoniae among 8 other different cucurbits tested. The SDS-PAGE patterns of total proteins from a susceptible (Keumcheon) and a resistant (PI 189225) watermelon cultivars were compared 0, 12, 24 and 36 hrs after inoculation. The amounts of two distinct protein bands (24 kDa and 70 kDa) were gradually increased after inoculation in both cultivars.

• Research in Plant Disease
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• v.23 no.2
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• pp.168-176
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• 2017

Root-dipping inoculation method has been widely used to determine the resistance of watermelon to Fusarium oxysporum f. sp. niveum causing Fusarium wilt. Although this method leads to the precise results of plant disease responses, more rapid and efficient assay methods have been still required because the root-dipping inoculation method is labor-intensive and time-consuming. In this study, we established a simple and effective bioassay method based on the comparison of various inoculation methods and growth conditions. To develop the system, the occurrence of Fusarium wilt on four resistant and susceptible cultivars was investigated by four different inoculation methods, root-dipping, scalpel, tip and soil-drenching methods. Of these inoculation methods, scalpel method resulted in clear plant disease resistance responses with the simplicity. With the use of scalpel method, we also explored the disease development of the cultivars depending on inoculum concentration, growth stage of seedlings, and incubation temperature after inoculation. Furthermore, we found that the resistance degrees of 23 cultivars derived by scalpel inoculation method were similar to the results by root-dipping method established previously.