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http://dx.doi.org/10.7745/KJSSF.2013.46.5.373

Effect of Dispersed and Proximate Inoculation Methods of Glomus etunicatum on Root Colonization of Sorghum-Sudangrass Hybrid  

Lee, Seonmi (Department of Agricultural Chemistry, Chungbuk National University)
Selvakumar, Gopal (Department of Agricultural Chemistry, Chungbuk National University)
Krishnamoorthy, Ramasamy (Department of Agricultural Chemistry, Chungbuk National University)
Kim, Kiyoon (Department of Agricultural Chemistry, Chungbuk National University)
Choi, Joonho (Department of Food Science and Biotechnology, College of Life Science and Natural Resources, Wonkwang University)
Sa, Tongmin (Department of Agricultural Chemistry, Chungbuk National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.46, no.5, 2013 , pp. 373-378 More about this Journal
Abstract
Information on the effective application method of arbuscular mycorrhizal fungi (AMF) inoculum is still inadequate. This work was performed to assess two AMF inoculation methods (dispersed and proximate) on root colonization of sorghum-sudangrass hybrid (Sorghum bicolor L.). In dispersed inoculation method, spores were inoculated in 2 kg pots of soil in which 5 day-old seedlings were transplanted and maintained for 50 days. In the proximate inoculation method, spores were first introduced in 500 mL pots where seeds were sown. After 10 days, the seedlings with the 500 mL soil were transferred to 2 kg pots without disturbing the contents. After 50 days of growth, root colonization and arbuscule abundance significantly increased (over 100%) in proximate method of inoculation. Moreover, sorghum-sudangrass hybrid had higher shoot growth (182.5 cm) and Glomalin related soil protein (GRSP) production in proximate method. Nutrient accumulation, particularly total nitrogen (82.61 mg $plant^{-1}$), was also found to be higher in proximate method of inoculation. Our results demonstrate that the proximate method of inoculation may improve the early stage mycorrhizal symbiosis and inoculum performance in Saemangeum reclaimed soil.
Keywords
Arbuscular mycorrhizal fungi; Glomalin; Inoculation methods; Plant growth; Saemangum reclaimed land;
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1 Adriano-Anaya, M.L., M. Salvador-Figueroa, J.A. Ocampo, and I. Garciya-Romera. 2006. Hydrolytic enzyme activities in maize (Zea mays) and sorghum (Sorghum bicolor) roots inoculated with Gluconacetobacter diazotrophicus and Glomus intraradices. Soil Biol. Biochem. 38:879-886.   DOI   ScienceOn
2 Al-Karaki, G.N. 2006. Nursery inoculation of tomato with arbuscular mycorrhizal fungi and subsequent performance under irrigation with saline water. Sci. Hortic. 109:1-7.   DOI   ScienceOn
3 Baird J.M., F.L. Walley, and S.J. Shirtliffe. 2010. Arbuscular mycorrhizal fungi colonization and phosphorus nutrition in organic field pea and lentil. Mycorrhiza 20:541-549.   DOI
4 Caravaca, F., M.M. Alguacil, J.M. Barea, and A. Roldan. 2005. Survival of inocula and native AM fungi species associated with shrubs in a degraded Mediterranean ecosystem. Soil Biol. Biochem. 37:227-233.   DOI   ScienceOn
5 Daei, G., M.R. Ardekani, F. Rejali, S. Teimuri, and M. Miransari. 2009. Alleviation of salinity stress on wheat yield, yield components, and nutrient uptake using arbuscular mycorrhizal fungi under field conditions. J. Plant Physiol. 166:617-625.   DOI   ScienceOn
6 Dubsky, M., F. Sramck, and M. Vosatka. 2002. Inoculation of cyclamen (Cyclamen persicum) and poinsettia (Euphorbia pulcherrima) with arbuscular mycorrhizal fungi and Trichoderma harzianum. Rost vyroba 48:63-68.
7 Estrada, B., R. Aroca, J. M. Barea, and J. M. Ruiz-Lozano. 2013. Native arbuscular mycorrhizal fungi isolated from a saline habitat improved maize antioxidant systems and plant tolerance to salinity. Plant Sci. 201-202:42-51.
8 Gadkar, V., R. David-Schwartz, T. Kunik, and Y. Kapulnik. 2001. Arbuscular mycorrhizal fungal colonization. Factors involved in host recognition. Plant Physiol. 127:1493-1499.   DOI
9 Gamalero, E., G. Berta, N. Massa, B.R. Glick, and G. Lingua. 2008. Synergistic interactions between the ACC deaminaseproducing bacterium Pseudomonas putida UW4 and the AM fungus Gigaspora rosea positively affect cucumber plant growth. FEMS Microbiol. Ecol. 64:459-467.   DOI   ScienceOn
10 Giananazzi, S., H. Schuepp, J.M. Barea, and K. Haselwandter. 2002. Mycorrhizal technology in agriculture: from genes to bioproducts. Birkhauser, Basel, Switzerland.
11 Hammer, E.C., and M.C. Rillig. 2011. The influence of different stresses on glomalin levels in an arbuscular mycorrhizal fungus-salinity increases glomalin content. PLoS One 6(12):1-5.
12 Lovato, P.E., V. Gininazzi-Pearoon, A. Trouvelot, and S. Gininazzi. 1996. The state of art of mycorrhizas and micropropagation. Adv. Hortic. Sci. 10:46-52.
13 Helgason, T., and A.H. Fitter. 2009. Natural selection and the evolutionary ecology of the arbuscular mycorrhizal fungi (Phylum Glomeromycota). J. Exp. Bot. 60:2465-2480.   DOI   ScienceOn
14 Jahromi, F., R. Aroca, R. Porcel, and J.M. Ruiz-Lozano. 2008. Influence of salinity on the in vitro development of Glomus intraradices and on the in vivo physiological and molecular responses of mycorrhizal lettuce plants. Microb. Ecol. 55: 45-53.   DOI
15 Khan, A.G. 2006. Mycorrhizoremediation-an enhanced form of phytoremediation. J. Zhejiang Univ. Sci. 7:503-514.   DOI   ScienceOn
16 Marschner, P. 2007. Plant-microbe interactions in the rhizosphere and nutrient cycling, p. 159-182. In: P. Marschner, and Z. Rengel (eds.), Nutrient cycling in terrestrial ecosystems. Part I. Book series: Soil biology, Berlin/Heidelberg, Germany.
17 Martin, F., S. Perotto, and P. Bonfante. 2007. Mycorrhizal fungi: A fungal community at the interface between soil and roots. p. 201-236. In: R. Pinton, Z. Varanini, and P. Nannipieri (eds.), The rhizosphere: Biochemistry and organic substances at the soil-plant interface. Marcel Dekker, New York.
18 Phillips, J.M., and D.S. Hayman. 1970. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Br. Mycol. Soc. 55:158-161.   DOI
19 Singh, H. 2006. Mycorrhizal fungi in rhizosophere bioremediation, p. 533-572. In: H. Singh (ed.), Mycoremediation: Fungal bioremediation. John, New York.
20 Smith, S.E., and D.J. Read. 1997. Mycorrhizal Symbiosis. Academic Press, London.
21 Smith, S.E., and D.J. Read. 2008. Mycorrhizal Symbiosis, third ed. Academic Press, San Diego, CA.
22 Solaiman, M.Z., and H. Hirata. 1997. Effect of arbuscular mycorrhizal fungi inoculation of rice seedlings at the nursery stage upon performance in the paddy field and greenhouse. Plant Soil 191:1-12.   DOI   ScienceOn
23 Temperini, O., Y. Rouphael, L. Parrano, E. Biagiola, G. Colla, R. Mariotti, E. Rea, and C.M. Rivera. 2009. Nursery inoculation of pepper with arbuscular mycorrhizal fungi: an effective tool to enhance transplant performance. Acta Hort. (ISHS) 807: 591-596.
24 Trouvelot, A., J.L. Kough, and V. Gianinazzi-Pearson. 1986. Mesure du taux de mycorhization VA dun systeme radiculaire. Recherche de methodes destimation ayant une signification fonctionnelle, p. 217-221. In: V. Gianinazzi-Pearson, and S. Gianinazzi, (eds.), Physiological and Genetical Aspects of Mycorrhizae. INRA Press, Paris.
25 Wang, X., Q. Pan, F. Chen, X. Yan, and H. Liao. 2011. Effects of co-inoculation with arbuscular mycorrhizal fungi and rhizobia on soybean growth as related to root architecture and availability of N and P. Mycorrhiza 21(3):173-181.   DOI   ScienceOn
26 Waterer, D.R., and R.R. Coltman 1988. Phosphorus concentration and application interval influence growth and mycorrhizal infection of tomato and onion transplants. J. Am. Soc. Hortic. Sci. 113:704-798.
27 Wright, S., and A. Upadhyaya. 1998. A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant Soil. 198: 97-107.   DOI   ScienceOn
28 Zubek, S., J. Blaszkowski, and P. Mleczko. 2011. Arbuscular mycorrhizal and dark septate endophyte associations of medicinal plants. Acta Soc. Bot. Pol. 80(4):285-292.   DOI
29 Zhu, X., F. Song, and H. Xu. 2010. Influence of arbuscular mycorrhiza on lipid peroxidation and antioxidant enzyme activity of maize plants under temperature stress. Mycorrhiza 20:325-332.   DOI
30 Gerdemann, J.W., and T.H. Nicolson. 1963. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc. 46(2):235-244.   DOI