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http://dx.doi.org/10.5423/PPJ.2003.19.5.257

Standardization of a Graft Inoculation Method for the Screening of Mungbean Germplasm against Mungbean yellow mosaic virus (MYMV)  

Akhtar, Khalid Pervaiz (Plant Health Group, Nuclear Institute for Agriculture and Biology)
Ahsanul Haq, M. (Plant Health Group, Nuclear Institute for Agriculture and Biology)
Publication Information
The Plant Pathology Journal / v.19, no.5, 2003 , pp. 257-259 More about this Journal
Abstract
This report described a simple, inexpensive, faster, and effective graft inoculation method for the artificial transmission of Mungbean yellow mosaic virus (MYMV). Success of grafting and disease transmission was 100% in this method. Screening of mungbean germplasm using this method will prevent the chance of escape infection, probably as a consequence of non-preference mechanism and loss of vector infectivity. The grafting method described here is applicable to both screenhouse and field trials.
Keywords
Germplasm; graft inoculation; mungbean; MYMV; screening; Vigna radiata;
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1 Ali, M. 2003. Mungbeans: Taiwan, China sharing innovative experiences (vol. 7) Mungbeans: Taiwan, China
2 Nair, N. G. and Nene, Y. L. 1973. Studies on the yellow mosaic of urdbean (Phaseolus mungo L.) caused by mungbean yellow mosaic virus. 2. virus-vector relationships. Indian J. Farm Science 1:62-70
3 Rachie, N. K. and Roberts, L. M. 1974. Grain legumes of low land tropics. Adv. Agron. 26:1-118   DOI
4 Matthews, R. E. F 1970. Methods of transmission and infection in 'Plant Virology'. Academic Press, New York and London. 778p.
5 Jones, D. R. 2003. Plant viruses transmitted by whiteflies. Eur. J. Plant Pathol. 109:195-219   DOI   ScienceOn
6 Ahmad, M. and Harwood, R. F. 1973. Studies on whitefly transmitted yellow mosaic disease of cowpea (Vigna unguiculata). Plant Dis. Rep. 62:224-226
7 Malik, I. A., Sarwar, G. and Ali, Y. 1986. Inheritance of tolerance to mungbean yellow mosaic virus and some morphological characters. Pak. J. Bot. 18:189-198
8 Nene, Y. L. 1973. Viral diseases of some warm weather pulse crops in India. Plant Dis. Rep. 57:463-467
9 Chenulu, V. V. and Verma, A. 1988. VIfUS and virus-like diseases of pulse crops commonly grown in India, pp. 338-370. In: Baldev B, Ramajunam S and Jain H K (eds.) Pulse Crops, New Delhi, Oxford and IBH
10 Malik, B. A. and Bashir, M. 1992. Major diseases of food legume crops of Islamic countries. In: Proceedings of COMSTECH-NIAB International Workshop on agroclimatology pests and diseases and their control by: Jamil, F.F. and Naqvi, S.H.M. pp.25-38
11 Honda, Y., Iwaki, M. and Saito, Y. 1983. Mechanical transmission, purification and some properties of whitefly-borne mungbean yellow mosaic virus in Thailand. Plant Dis. 67: 801-804   DOI
12 Akhtar, K. P., Khan, A. I. and Khan, M. S. I. 2001. Response of some cotton varieties to leaf curl virus through grafting. Pak. J. Phytopathol. 13:91-95