• 벤처창업연구
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• 제10권1호
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• pp.95-110
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• 2015

기업가정신이론을 비롯하여 많은 혁신이론들이 경영자의 혁신능력을 혁신의 출발로 보고 있다. 본 연구는 경영자의 혁신DNA라는 역설적 은유를 통해 혁신에 미치는 영향과 환경의 적합성 관계를 규명함으로써 학습과 노력에 의해 후천적으로 혁신역량을 높일 수 있는 구체적인 방향을 모색하는데 목적을 두고 있다. 이를 위해서 대구경북 110개 제조기업을 대상으로 자료를 수집하여 실증분석을 하였다. 실증분석 결과 혁신DNA는 혁신에 전반적으로 긍정적인 영향을 미치는 것을 확인하였다. 특히 발견DNA는 실행DNA보다 제품전략에 더 강하게 작용하는 반면에, 실행DNA는 공정혁신에 더 강하게 작용하는 것으로 나타났다. 혁신DNA와 환경의 적합성에 따른 혁신의 영향의 경우, 발견DNA와 기술격변성은 상호적합성을, 시장격변성은 보완적합성을 통해 제품혁신을 강화하는 것으로 나타났다. 실행DNA와 시장격변성의 상호적합성을 통해 공정혁신을 강화시키는 것으로 나타났다.

• Fisheries and Aquatic Sciences
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• 제27권2호
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• pp.87-99
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• 2024

Global climate change, followed by an increase in anthropogenic activities in aquatic ecosystems, and species invasions, has resulted in a decline in aquatic organism biodiversity. The Batanghari River, Sumatra's longest river, is polluted by mercury-containing illegal gold mining waste (PETI), industrial pollution, and domestic waste. Several studies have provided evidence suggesting a decline in fish biodiversity within the Batanghari River. However, a comprehensive evaluation of the present status of biodiversity in this river is currently lacking. The species under investigation were identified through various molecular-based identification methods, as well as morphological identification, which involved the use of neighbor-joining (NJ) trees. All collected specimens were initially identified using morphological techniques and subsequently confirmed with molecular barcoding analysis. Morphological and DNA barcoding identification categorized all specimens (1,692) into 36 species, 30 genera and 16 families, representing five orders. A total of 36 DNA barcodes were generated from 30 genera using a 650-bp-long fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene. Based on the Kimura two-parameter model (K2P), The minimum and maximum genetic divergences based on K2P distance were 0.003 and 0.331, respectively, and the average genetic divergence within genera, families, and orders was 0.05, 0.12, 0.16 respectively. In addition, the average interspecific distance was approximately 2.17 times higher than the mean intraspecific distance. Our results showed that the COI barcode enabled accurate fish species identification in the Batanghari River. Furthermore, the present work will establish a comprehensive DNA barcode library for freshwater fishes along Batanghari River and be significantly useful in future efforts to monitor, conserve, and manage fisheries in Indonesia.

• 벤처창업연구
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• 제9권6호
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• pp.199-212
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• 2014

최근 글로벌 기업들의 성공 혁신사례들에 대한 관심이 혁신의 원천인 DNA관점에서 접근이 진행되고 있다. 본 연구는 이러한 글로벌 대기업의 사례가 중소기업의 경영자 측면에서 적용되는가를 규명하고자 한다. 특히 중소기업의 혁신전략 관점에서 혁신DNA에 어떻게 작용하는가를 실증적으로 밝히는데 목적을 두었다. 실증연구는 대구경북 110개 기업으로부터 수집된 전반에 더 강하게 작용하는 것을 확인하였다. 실행DNA 또한 시장차별화 전략에 긍정적인 영향을 미치지만, 발견DNA와 동시에 고려할 때 제품차별화에 부정적인 영향을 미치는 것으로 나타났다. 하위 구성요소 측면에서는 발견DNA의 질문하기와 연자료를 통해서 실시하였다. 본 연구의 결과 혁신DNA는 혁신전략에 영향을 미치며, 특히 발견DNA가 실행DNA보다 혁신전략결하기가 혁신전략 전반에, 실행DNA의 분석하기는 시장차별화 전략에 긍정적인 영향을 미치나, 세부 업무 추진하기는 제품차별화에 부정적으로 작용하였다. 본 연구의 결과는 글로벌 성공기업 사례에서와 같이 중소기업 경영자의 혁신DNA의 논리가 전반적으로 적용될 수 있음을 확인하였으며, 중소기업의 혁신전략에 있어서 발견DNA의 중요성과 더불어 이를 제고하기 위한 학습노력이 중요하다는 것을 시사한다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.361-366
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• 2005

Opium poppy (Papaver somniferum L.) is one of the most important medicinal plants, which is used as a sole commercial source of narcotic analgesic, morphine. The transformant of opium poppy we have established by infection of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) strain MAFF03-01724 showed aberrant morphology and altered opium alkaloid composition. The major alkaloid produced by this transformant was thebaine (16.3%, opium dry weight) instead of morphine. It is likely that this 'thebaine poppy' phenotype was caused by the integration of T-DNA(s) into the poppy genome DNA, and their inserted loci are of great interest. To gain an insight into the mechanism of nonnarcotic thebaine accumulation for the further approach toward the creation of 'codeine poppy' which produces codeine as a major alkaloid, the genetical and morphological analyses on the transformant was carried out. Here we report the results of the detailed analysis on the T-DNA inserted loci of T0 transfromant and the correlation between opium alkaloid composition and segregated T-DNA integration pattern in the self-pollinated T1 transformants.

• 한국기술혁신학회:학술대회논문집
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• 한국기술혁신학회 2013년도 추계 학술대회 초록집
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• pp.843-843
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• 2013

• 한국미생물·생명공학회지
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• 제50권3호
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• pp.328-336
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• 2022

Aptamers are short, chemically synthesized, single-stranded DNA or RNA oligonucleotides that fold into unique three-dimensional structures. In this study, we aim to determine the antibiofilm activity and binding specificity of the six polyclonal DNA aptamers (S15K3, S15K4, S15K6, S15K13, S15K15, and S15K20) on Staphylococcus aureus BPA-12 and Escherichia coli EPEC 4. Aptamer S15K6 showed the highest percentage of antibiofilm activity against S. aureus BPA-12 (37.4%) as shown by the lowest OD570 value of 0.313. Aptamer S15K20 showed the highest percentage of antibiofilm activity against E. coli EPEC 4 (15.4%) as shown by the lowest OD570 value of 0.515. Aptamers S15K13 and S15K20 showed antibiofilm activities against both S. aureus BPA-12 and E. coli EPEC4, and thus potentially have broad reactivity. Furthermore, based on the binding capacity and Kd values from our previous study, the binding specificity assay of selected polyclonal DNA aptamers (S15K3 and S15K15) against S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, S. agalactiae, E. coli MHA-6, and Listeria monocytogenes were performed using qPCR. Aptamers S15K3 and S15K15 showed specific binding to S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, and S. agalactiae, but could not bind to E. coli MHA-6 and L. monocytogenes. Therefore, this study showed that the polyclonal DNA aptamers have antibiofilm activity and were able to bind to S. aureus BPA-12 and E. coli EPEC 4 bacteria.

• Fisheries and Aquatic Sciences
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• 제27권3호
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• pp.159-170
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• 2024

Research to obtain molecular markers related to the major histocompatibility complex (MHC) gene in both strains of gourami is essential to increase the success of the selection program of disease resistance traits. Using a completely randomized design (CRD), the challenge test consists of four treatments and seven replications. The treatment was Jambi gourami injected with PBS (KJ), Kalimantan gourami injected with PBS (KK), Jambi strain injected with Aeromonas hydrophila (GJ), and Kalimantan strain injected with A. hydrophila (GK). The GJ population was more resistant to A. hydrophila than the GK population. The MHC II gene was detected in both test strains (GJ and GK), both resistant and susceptible fish. However, there were differences in the results of amplifying the MHC II gene in susceptible and resistant fish. Two DNA fragments approximately 400 and 585 bp were detected in the genome of susceptible fish, while in the genome of susceptible fish, only one DNA fragment was detected (400 bp). Therefore, the MHC II gene fragment with a size of about 585 bp can be used as a potential candidate for specific molecular markers to obtain resistance to A. hydrophila bacteria in the giant gourami.

• 한국식품영양과학회지
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• 제27권6호
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• pp.1166-1172
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• 1998

A method for the isolation and purification of DNA from a red algae, Porphyra was innovated. The innovation of the method consists mainly of three steps that include sodium acetate treatment, chloroform extraction, and 0.2 volume isopropanol precipitation step. The sodium acetate treatment was designed to remove polysaccharide contamination, and the isopropanol step to remove proteins and salts contaminents. Genomic DNA,s of several species(for example, P. tenera, P. yezoensis, P. seriata, and P. pseudolinearis) was successfully isolated by the innovated method. The amount of DNA purified from one g of sample material with the innovated method was 53 g in average. The resulting DNA was characterized to include high molecular weight and showed no nuclease activity. The DNA was pure enough to be digested directly by various restriction enzymes without any difficulties. Porphyra DNA was pure enough and adequate for amplification reaction through the polymerase chain reaction (small nuclear rDNA PCR amplification).

• 기술혁신연구
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• 제9권1호
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• pp.21-35
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• 2001

The main purpose of this study is to measure and compare the state of the art (SOA) of DNA chips of Korea, Japan and the United States using Gordon's scoring model. From the comparison, Korea's SOAs of DNA chip were estimated to be 70% and 62% of those of the United States in terms of functional and technical parameters, whereas Japan's SOAs were 79% and 77%, respectively. The results of this study could be applied to the strategic technology planning for narrowing the technology gap, and used as one of the key criteria for resource allocation in national R&D programs and the fundamental information to formulate the biotechnology policy for the Korean government.

• 기술혁신학회지
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• 제2권3호
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• pp.94-106
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• 1999

In Korea Basic Science Institute(KBSI) the remote data analysis system is developed for the joint use of advanced equipments. This system enables the researchers to access the datas which are produced at KBSI and analyse them by Java program on the Web,. Except Web browser such as Internet Explorer or Netscape Navigator no additional softwares are required for analysing data. We have developed remote data analysis systems for five major equipments which KBSI supports for the researchers, The systems which are developed are those for NMR spectrometer High Reso-lution Tandem mass Spectrometer Microscopic Imaging System DNA Sequencer and Natural Ra-dioactivity Measruement System, These programs work on any computer platform and any operat-ing system only if the internet is available. This remote data analysis system will be served as a part of Collaboratory the remote collaborative system.