[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.48022/mbl.2206.06001

Antibiofilm Activity and Binding Specificity of Polyclonal DNA Aptamers on Staphylococcus aureus and Escherichia coli

Arizah Kusumawati (Study Program of Veterinary Public Health, IPB Graduate School, IPB University)
Apon Zaenal Mustopa (Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN))
Rifqiyah Nur Umami (Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN))
Adi Santoso (Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN))
I Wayan Teguh Wibawan (Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University)
Agus Setiyono ( Department of Veterinary Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, IPB University)
Mirnawati Bachrum Sudarwanto (Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University)

Publication Information

Microbiology and Biotechnology Letters / v.50, no.3, 2022 , pp. 328-336 More about this Journal

Abstract

Aptamers are short, chemically synthesized, single-stranded DNA or RNA oligonucleotides that fold into unique three-dimensional structures. In this study, we aim to determine the antibiofilm activity and binding specificity of the six polyclonal DNA aptamers (S15K3, S15K4, S15K6, S15K13, S15K15, and S15K20) on Staphylococcus aureus BPA-12 and Escherichia coli EPEC 4. Aptamer S15K6 showed the highest percentage of antibiofilm activity against S. aureus BPA-12 (37.4%) as shown by the lowest OD570 value of 0.313. Aptamer S15K20 showed the highest percentage of antibiofilm activity against E. coli EPEC 4 (15.4%) as shown by the lowest OD570 value of 0.515. Aptamers S15K13 and S15K20 showed antibiofilm activities against both S. aureus BPA-12 and E. coli EPEC4, and thus potentially have broad reactivity. Furthermore, based on the binding capacity and Kd values from our previous study, the binding specificity assay of selected polyclonal DNA aptamers (S15K3 and S15K15) against S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, S. agalactiae, E. coli MHA-6, and Listeria monocytogenes were performed using qPCR. Aptamers S15K3 and S15K15 showed specific binding to S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, and S. agalactiae, but could not bind to E. coli MHA-6 and L. monocytogenes. Therefore, this study showed that the polyclonal DNA aptamers have antibiofilm activity and were able to bind to S. aureus BPA-12 and E. coli EPEC 4 bacteria.

Keywords

DNA aptamer; antibiofilm; qPCR; 16S rRNA analysis;

Citations & Related Records

Times Cited By KSCI : 11 (Citation Analysis)

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